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Cellular & Molecular Immunology 2022年第12期19卷 1392-1399页

作者： Lunzhi Yuan Huachen zhu Peiwen chen Ming zhou Jian Ma Xuan Liu Kun Wu Rirong chen Qiwei Liu Huan Yu Lifeng Li Jia Wang Yali zhang Shengxiang Ge Quan Yuan Qiyi Tang Tong cheng Yi Guan Ningshao Xia State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious DiseasesSchool of Life SciencesSchool of Public HealthXiamen UniversityXiamenFujianChina State Key Laboratory of Emerging Infectious Diseases School of Public HealthLi Ka Shing Faculty of MedicineThe University of Hong KongHong Kong SARChina Guangdong-Hong Kong Joint Laboratory of Emerging Infectious Diseases/Joint Laboratory for International Collaboration in Virology and Emerging Infectious Diseases Joint Institute of Virology(STU/HKU)Shantou UniversityShantouGuangdongChina EKIH Pathogen Research institute Futian DistrictShenzhenGuangdongChina Department of Microbiology Howard University College of MedicineWashingtonDCUSA

The new predominant circulating SARS-CoV-2.variant,Omicron,can robustly escape current vaccines and neutralizing *** Omicron has been reported to have milder replication and disease manifestations than some earlier variants,its pathogenicity in different age groups has not been well ***,we report that the SARS-CoV-2.Omicron BA.1 sublineage causes elevated infection and lung pathogenesis in juvenile and aged hamsters,with more body weight loss,respiratory tract viral burden,and lung injury in these hamsters than in adult *** hamsters show a reduced interferon response against Omicron BA.1 infection,whereas aged hamsters show excessive proinflammatory cytokine expression,delayed viral clearance,and aggravated lung *** inhaled IFN-α2. treatment suppresses Omicron BA.1 infection and lung pathogenesis in juvenile and adult ***,the data suggest that the diverse patterns of the innate immune response affect the disease outcomes of Omicron BA.1 infection in different age groups.

关键词： SARS-CoV-2.Omicron Lung pathology Innate immune response Interferon treatment

Development of New InDel Marker to Detect Genotypes of Rf-1a Conferring Fertility Restoration of BT-Type Cytoplasmic Male Sterility in Rice

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Rice science 2014年第1期21卷 13-19页

作者： chen Tao zhang Ya-dong {2. Zhen ZHAO Ling ZHAO Qing-yong zhou Li-hui YAO Shu YU Xin WANG Cai-lin institute of Food Crops Jiangsu Academy of Agricultural Sciences / Jiangsu High Quality Rice R & D Center / Nanjing Branch of China National Center for Rice Improvement

Restorer line breeding is an important approach to enhance the heterosis and improve the yields of japonica hybrid rice. To improve the selection efficiency of restorer lines for BT-type cytoplasmic male sterility （CMS） in japonica rice, a functional marker InDeI-Rf-la based on the difference of nucleotide sequence in Rf-la locus between BT-type CMS lines and restorer lines was developed to detect the genotypes of different rice materials. Conventional indica rice varieties, restorer and maintainer lines without 574 bp deletion could restore the fertility for BT-type CMS in japonica rice. By contrast, most conventional japonica rice varieties except Aichi 106 and Yijing 12. with genotype of rf-larf-la showed the 574 bp deletion maintained sterility for BT-type CMS lines. To further verify the effect of genotyping detection in Rf-la locus, this marker was also used to amplify the genomic DNA in different japonica rice restorer lines, CMS lines, hybrids and F2.segregation population, and three genotypes in Rf-la locus could be distinguished distinctly. Therefore, the marker InDeI-Rf-la could be widely used for genetic id^ntifio.~tinn ~nd m^rkp.r-~.~.~i^fp.d .~.*** （MA.~ in hr~=dinn i^nnnir~ r^fnr~=r lin==~

关键词： BT-type cytoplasmic male sterility fertility restoration Rf-la gene InDel marker japonica rice

胆囊癌根治性目的切除术达到教科书式结局对远期预后影响的全国多中心队列研究

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中华消化外科杂志 2024年第7期23卷 926-933页

作者：刘智鹏李子沐罗宇乐赵小林白洁江艳李云峰喻超黄帆吴兆平周进学尹大龙丁睿郭伟朱沂陈伟林科灿岳平程遥戴海粟张东陈志宇陆军军医大学第一附属医院肝胆外科全军肝胆外科研究所重庆400038 湖南省人民医院肝胆胰外科长沙410002 贵州医科大学附属医院肝胆外科贵阳550004 安徽医科大学第一附属医院普通外科合肥230022 九江市第一人民医院肝胆胰外科九江332001 郑州大学附属肿瘤医院肝胆胰外科郑州450003 中国科学技术大学附属第一医院肝胆外科合肥230002 空军军医大学第一附属医院肝胆胰外科西安710032 首都医科大学附属北京友谊医院普通外科北京100050 浙江大学医学院附属第二医院肝胆胰外科杭州310003 中山大学第一附属医院肝胆胰外科中山510080 福建医科大学孟超肝胆医院肝病中心福州350025 兰州大学第一医院普通外科兰州730013 重庆医科大学附属第二医院肝胆外科重庆400010 西安交通大学第一附属医院肝胆外科西安710061

目的探讨胆囊癌根治性目的切除术后达到肝脏外科中教科书式结局(TOLS)对远期预后的影响。方法采用回顾性队列研究方法。收集2.14.1月至2.2.年1月中华消化外科菁英荟胆道外科学组全国多中心数据库15家医学中心收治的82.例胆囊癌患者的... 详细信息

目的探讨胆囊癌根治性目的切除术后达到肝脏外科中教科书式结局(TOLS)对远期预后的影响。方法采用回顾性队列研究方法。收集2.14.1月至2.2.年1月中华消化外科菁英荟胆道外科学组全国多中心数据库15家医学中心收治的82.例胆囊癌患者的临床病理资料;男2.5例,女539例;年龄为(62.11)岁。根据TOLS评价标准,将患者分为达到TOLS和未达到TOLS。正态分布的计量资料以x±s表示,组间比较采用独立样本t检验;偏态分布的计量资料以M(Q1,Q3)表示,组间比较采用Mann‑Whitney U检验。计数资料以绝对数表示,组间比较采用χ^(2.检验。等级资料比较采用Mann‑Whitney U检验。采用Kaplan‑Meier法计算生存率和绘制生存曲线,Log‑rank检验进行生存分析。单因素分析和多因素分析采用COX逐步回归模型向后Wald法。结果(1)TOLS情况。82.例行胆囊癌根治性目的切除术患者中,510例达到TOLS,314例未达到TOLS。(2.随访情况。82.例行胆囊癌根治性目的切除术患者,排除出院后90 d内死亡的112.,712.患者纳入生存分析。510例达到TOLS患者的中位随访时间为2..1(11.4,30.1)个月,中位总生存时间为47.6(30.6,64.6)个月,5年总生存率为47.5%。2.2.未达到TOLS患者的中位随访时间为14.0(6.8,2..5)个月,中位总生存时间为2..3(2..0,2..6)个月,5年总生存率为2..0%。两者总生存情况比较,差异有统计学意义(χ^(2.=58.4.1,P3)影响患者生存情况的因素分析。多因素分析结果显示:TOLS、癌胚抗原、CA19‑9、肿瘤分化程度为低分化、第八版美国癌症联合委员会(AJCC)T2.、第八版AJCC T3期和T4期、第八版AJCC N1期、第八版AJCC N2.、辅助治疗是胆囊癌根治性目的切除术后患者总生存时间的独立影响因素(风险比=0.452.1.479,1.373,1.612.1.455,1.4.1,1.835,1.978,0.538,95%可信区间为0.352.0.581,1.141~1.964.1.052.1.791,1.2.9~2.063,1.102.1.92.,1.02.~2.147,1.380~2.4.1,1.342.2.915,0.382.0.758,P<0.05)。结论达到TOLS的胆囊癌根治性目的切除术患者能够获得更好的远期预后。

关键词：胆道肿瘤肝切除教科书式结局影响因素分析预后

Significant loss of soil inorganic carbon at the continental scale

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National Science Review 2022年第2期9卷 13-20页

作者： Xiao-Dong Song Fei Yang Hua-Yong Wu Jing zhang De-cheng Li Feng Liu Yu-Guo Zhao Jin-Ling Yang Bing Ju Chong-Fa Cai Biao Huang Huai-Yu Long Ying Lu Yue-Yu Sui Qiu-Bing Wang Ke-Ning Wu Feng-Rong zhang Ming-Kui zhang zhou Shi Wan-zhu Ma Gang Xin Zhi-Ping Qi Qing-Rui Chang En Ci Da-Gang Yuan Yang-zhu zhang Jun-Ping Bai Jia-Ying chen Jie chen Yin-Jun chen Yun-Zhong Dong Chun-Lan Han Ling Li Li-Ming Liu Jian-Jun Pan Fu-Peng Song Fu-Jun Sun Deng-Feng Wang Tian-Wei Wang Xiang-Hua Wei Hong-Qi Wu Xia Zhao Qing zhou Gan-Lin zhang State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences College of Advanced Agricultural sciences University of Chinese Academy of Sciences College of Resources and Environment Huazhong Agricultural University institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences College of Natural Resources and Environment South China Agricultural University Northeast institute of Geography and Agroecology Chinese Academy of Sciences College of Land and Environment Shenyang Agricultural University School of Land Science and Technology China University of Geosciences College of Land Science and Technology China Agricultural University College of Environmental and Resource sciences Zhejiang University institute of Digital Agriculture Zhejiang Academy of Agricultural Sciences College of Agriculture Heilongjiang Bayi Agricultural University Tropical Crops Genetic Resources institute Chinese Academy of Tropical Agricultural Sciences College of Natural Resources and Environment Northwest A & F University College of Resources and Environment Southwest University College of Resources Sichuan Agricultural University College of Resources and Environment Hunan Agricultural University institute of Agricultural Product Quality Standard and Testing Research Tibet Academy of Agricultural and Animal Husbandry Sciences School of Agricultural sciences Zhengzhou University institute of Agriculture Environment and Resources Research Shanxi Academy of Agricultural Sciences College of Resources and Environment Henan Agricultural University College of Resources and Environment China Agricultural University College of Resources and Environmental sciences Nanjing Agricultural University College of Resources and Environment Shandong Agricultural University Agricultural College Guangxi University College of Grassland and Environment Science Xinjiang Agricultural University College of Geographical Science Qinghai Normal University

Widespread soil acidification due to atmospheric acid deposition and agricultural fertilization may greatly accelerate soil carbonate dissolution and ***,to date,few studies have addressed these ***,we use meta-analysis and nationwide-survey datasets to investigate changes in soil inorganic carbon(SIC) stocks in *** observe an overall decrease in SIC stocks in topsoil(0-30 cm)(11.33 g C m-2.r-1) from the 1980 s to the 2.10 *** SIC stocks have decreased by～8.99±2.2.%(1.37±0.37 Pg C).The average SIC losses across china(0.046 Pg C yr-1) and in cropland(0.016 Pg C yr-1) account for～17.6%-2..0% of the terrestrial C sink and 57.1% of the soil organic carbon sink in cropland,*** deposition and climate change have profound influences on SIC *** estimate that～19.12.-19.47% of SIC stocks will be further lost by *** consumption of SIC may offset a large portion of global efforts aimed at ecosystem carbon sequestration,which emphasizes the importance of achieving a better understanding of the indirect coupling mechanisms of nitrogen and carbon cycling and of effective countermeasures to minimize SIC loss.

关键词： china soil inorganic carbon stocks global change carbonate soil acidification

Dihydroergotamine ameliorates liver fibrosis by targeting transforming growth factor β type Ⅱ receptor

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World Journal of Gastroenterology 2023年第20期29卷 3103-3118页

作者： Ke-Xin Zheng Shou-Li Yuan Meng Dong Han-Lin zhang Xiao-Xiao Jiang Chun-Long Yan Rong-Cai Ye Hui-Qiao zhou Li {2. Rui Jiang Zi-Yu {2.g Zhi zhang Qi Wang Wan-zhu Jin Wen Xie Center of Liver Diseases Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China Key Laboratory of Animal Ecology and Conservation Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Graduate School University of the Chinese Academy of SciencesBeijing 100049China Graduate School Agriculture College of Yanbian UniversityYanji 133002Jilin ProvinceChina

BACKGROUND The transforming growth factor β(TGFβ) signaling pathway plays a crucial role in the development of liver fibrosis by activating TGFβ type Ⅱ receptor(TGFβR2., followed by the recruitment of TGFβR1 finally triggering downstream signaling *** To find drugs targeting TGFβR2.that inhibit TGFβR1/TGFβR2.complex formation, theoretically inhibit TGFβ signaling pathway, and thereby ameliorate liver *** Food and Drug Administration-approved drugs were screened for binding affinity with TGFβR2.by virtual molecular docking. We identified 6 candidates and further explored their potential by Cell Counting Kit-8(CCK-8) cell cytotoxic experiment to validate toxicity and titrated the best cellular working concentrations. Next, we further demonstrated the detailed molecular working mechanisms using mutagenesis analysis. Finally, we used a mouse model to investigate its potential anti-liver fibrosis *** We identified 6 drug candidates. Among these 6 drugs, dihydroergotamine(DHE) shows great ability in reducing fibrotic gene expressions such as collagen, p-SMAD3, and α-SMA in TGFβ induced cellular model of liver fibrosis in LX-2.cells. Furthermore, we demonstrated that DHE binds to TGFβR2. Moreover, mutation of Leu2., Phe30, Thr51, Ser52. Ile53, and Glu55 of TGFβR2.disrupted the binding of TGFβR2.with DHE. In addition, DHE significantly improved liver fibrosis, as evidenced by Masson’s trichrome staining of liver sections. This is further supported by the width and the velocity of the portal vein, and serum markers of liver function. In line with those observations, DHE also decreased macrophages infiltration and extracellular matrix deposition in the *** DHE alleviates liver fibrosis by binding to TGFβR2.thereby suppressing TGFβ signaling pathway. We show here that as far as drug repurposing, DHE has great potential to treat liver fibrosis.

关键词： Liver fibrosis Transforming growth factorβ(TGFβ)signaling pathway TGFβtype II receptor(TGFβR2. Virtual screening Drug-repurposing Dihydroergotamine

Marsdeniae tenacissimae extract(MTE) suppresses cell proliferation by attenuating VEGF/VEGFR2.interactions and promotes apoptosis through regulating PKC pathway in human umbilical vein endothelial cells

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chinese Journal of Natural Medicines 2016年第12期14卷 922-930页

作者： chen Bing-Yu chen Dong LYU Jian-Xin LI Kai-Qiang JIANG Meng-Meng ZENG Jing-Jing HE Xu-Jun HAO Ke TAO Hou-Quan MOU Xiao-{2. YING You-Min zhang Wei zhu Meng-Hua WANG Zhen Research Center of Blood Transfusion Medicine Education Ministry Key Laboratory of Laboratory Medicine Zhejiang Provincial People's Hospital Hangzhou 310014 China Wenzhou Center for Disease Control and Prevention Wenzhou 325001 China College of Pharmaceutical Science Zhejiang University of Technology Hangzhou 310014 China School of Laboratory Medicine and Life Science Wenzhou Medical University Wenzhou 325000 China Clinical Research institute Zhejiang Provincial People's Hospital Hangzhou 310014 China

Marsdeniae tenacissimae extract(MTE), commonly known as Xiao-Ai-Ping in china, is a traditional chinese herb medicine capable of inhibiting proliferation and metastasis and boosting apoptosis in various cancer cells. However, little is known about the contribution of MTE towards tumor angiogenesis and the underlying mechanism. The present study aimed to evaluate the effects of MTE on the proliferation and apoptosis of human umbilical vein endothelial cells(HUVECs) and the molecular ism. 3-(4,5-dimethylthiazol-2.yl)-5(3-carboxymethoxyphenyl)-2.(4-sulfopheny)-2.-tetrazolium, inner salt(MTS) and PI-stained flow cytometry assays revealed that MTE dose-dependently reduced the proliferation of HUVECs by arresting cell cycle at S phase(P 2.expression; caspase-3 was activated simultaneously in a dose-dependent manner(P 2.VEGFR-2., P2.6 receptor(P2.6R), and chemokine(C-C motif) ligand 2.CCL-2., along with the activation of PKC δ and up-regulation of p53 in a dose-dependent manner in MTE-treated selected cells(P 2.mediated VEGF/VEGFR2.interactions and promoted the apoptosis through PKCδ-induced p53-dependent mitochondrial pathway in HUVECs, supporting that MTE may be developed as a potent anti-cancer medicine.

关键词： Marsdeniae tenacissimae extraction Cancer Angiogenesis Proliferation Apoptosis

High threshold voltage enhancement-mode GaN p-FET with Sirich LPCVD SiN_(x) gate insulator for high hole mobility

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Journal of Semiconductors 2023年第8期44卷 78-86页

作者： Liyang zhu Kuangli chen Ying Ma Yong Cai Chunhua {2. Zhaoji Li Bo zhang Qi {2. State Key Laboratory of Electronic Thin Films and Integrated Devices University of Electronic Science and Technology of ChinaChengdu 610054China Key Laboratory of Nanodevices and Applications Suzhou Institute of Nano-tech and Nano-bionicsCASSuzhou 215123China institute of Electronic and Information Engineering University of Electronic Science and Technology of ChinaDongguan 523808China

In this work,the GaN p-MISFET with LPCVD-SiN_(x) is studied as a gate dielectric to improve device *** changing the Si/N stoichiometry of SiN_(x),it is found that the channel hole mobility can be effectively enhanced with Si-rich SiN_(x) gate dielectric,which leads to a respectably improved drive current of GaN *** record high channel mobility of 19.4 cm2.(V∙s)was achieved in the device featuring an Enhancement-mode *** from the significantly improved channel mobility,the fabricated E-mode GaN p-MISFET is capable of delivering a decent-high current of 1.6 mA/mm,while simultaneously featuring a negative threshold-voltage(VTH)of–2.3 V(defining at a stringent criteria of 10μA/mm).The device also exhibits a well pinch-off at 0 V with low leakage current of 1 nA/*** suggests that a decent E-mode operation of the fabricated p-FET is *** addition,the VTH shows excellent stability,while the threshold-voltage hysteresisΔVTH is as small as 0.1 V for a gate voltage swing up to–10 V,which is among the best results reported in the *** results indicate that optimizing the Si/N stoichiometry of LPCVD-SiN_(x) is a promising approach to improve the device performance of GaN p-MISFET.

关键词： p-channel GaN p-FET LPCVD channel mobility hole mobility enhancement-mode

Six steps for building a technological knowledge base for future taxonomic work

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National Science Review 2022年第12期9卷 19-21页

作者： Michael C.Orr Anderson Feijó Douglas Chesters Alfried P.Vogler Silas Bossert Rafael R.Ferrari Mark John Costello Alice C.Hughes Lars Krogmann John S.Ascher Xin zhou De-zhu Li Ming Bai Jun {2. Deyan Ge Arong Luo Gexia Qiao Paul H.Williams Ai-bing zhang Keping Ma Feng zhang Chao-Dong zhu Key Laboratory of Zoological Systematics and Evolution Institute of Zoology Chinese Academy of Sciences Entomologie Staatliches Museum für Naturkunde Stuttgart Department of Life sciences Silwood Park Campus Imperial College London Natural History Museum Department of Entomology Washington State University Department of Entomology National Museum of Natural History Smithsonian Institution Faculty of Biosciences and Aquaculture Nord University School of Biological sciences University of Hong Kong Department of Biological sciences National University of Singapore Department of Entomology China Agricultural University Germplasm Bank of Wild Species Kunming Institute of Botany Chinese Academy of Sciences College of Life sciences Capital Normal University institute of Botany Chinese Academy of Sciences Department of Entomology College of Plant Protection Nanjing Agricultural University State Key Laboratory of Integrated Pest Management Institute of Zoology Chinese Academy of Sciences College of Life sciences University of Chinese Academy of Sciences

Species are the principal category we use to study,understand,and conserve the natural world,so how we define species has a cascading impact across fields,well beyond biology [1].Despite such importance,debate on species concepts continues to this day ([2. 2.2.),with consequences for what organisms we recognize,study and *** the wealth of life forms on our planet,

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SARS-CoV-2.nucleocapsid protein triggers hyperinflammation via protein-protein interaction-mediated intracellular Cl^(−) accumulation in respiratory epithelium

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Signal Transduction and Targeted Therapy 2022年第8期7卷 3080-3092页

作者： Lei chen Wei-Jie Guan zhuo-Er Qiu Jian-Bang Xu Xu Bai Xiao-Chun Hou Jing Sun Su Qu Ze-Xin Huang Tian-Lun Lei Zi-Yang Huang Jincun Zhao Yun-Xin zhu Ke-Nan Ye Zhao-Rong Lun Wen-liang zhou Nan-Shan Zhong Yi-Lin zhang School of Life sciences Sun Yat-sen UniversityGuangzhouChina State Key Laboratory of Respiratory Disease National Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory HealthThe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Medical UniversityGuangzhouChina Department of Thoracic Surgery Guangzhou Institute for Respiratory HealthThe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Medical UniversityGuangzhouChina Guangzhou Laboratory GuangzhouChina

SARS-CoV-2.the culprit pathogen of COVID-19,elicits prominent immune responses and cytokine *** Cl^(−)is a crucial regulator of host defense,whereas the role of Cl^(−)signaling pathway in modulating pulmonary inflammation associated with SARS-CoV-2.infection remains *** using human respiratory epithelial cell lines,primary cultured human airway epithelial cells,and murine models of viral structural protein stimulation and SARS-CoV-2.direct challenge,we demonstrated that SARS-CoV-2.nucleocapsid(N)protein could interact with Smad3,which downregulated cystic fibrosis transmembrane conductance regulator(CFTR)expression via *** intracellular Cl^(−)concentration([Cl^(−)]i)was raised,resulting in phosphorylation of serum glucocorticoid regulated kinase 1(SGK1)and robust inflammatory *** or knockout of SGK1 abrogated the N protein-elicited airway ***,N protein promoted a sustained elevation of[Cl^(−)]i by depleting intracellular cAMP via upregulation of phosphodiesterase 4(PDE4).Rolipram,a selective PDE4 inhibitor,countered airway inflammation by reducing[Cl^(−)]*** findings suggested that Cl^(−)acted as the crucial pathological second messenger mediating the inflammatory responses after SARS-CoV-2.*** the Cl^(−)signaling pathway might be a novel therapeutic strategy for COVID-19.

关键词： inflammation respiratory epithelium