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甲状腺激素受体相互作用物11基因变异所致软骨生成不全IA型家系遗传学分析

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生殖医学杂志 2024年第4期33卷 466-472页

作者：陈慧芳张钏周秉博陈雪惠玲甘肃省妇幼保健院医学遗传中心甘肃省出生缺陷与罕见病临床研究中心兰州730050 甘肃中医药大学公共卫生学院兰州730000

软骨生成不全IA型是一种罕见的致死性疾病,以常染色体隐性方式遗传,该疾病的发生与人14号染色体上甲状腺激素受体相互作用物11(TRIP11)基因变异有关。本研究采集了1例超声检查疑似致命性骨骼发育不良胎儿的流产皮肤组织及其父母的静脉血... 详细信息

软骨生成不全IA型是一种罕见的致死性疾病,以常染色体隐性方式遗传,该疾病的发生与人14号染色体上甲状腺激素受体相互作用物11(TRIP11)基因变异有关。本研究采集了1例超声检查疑似致命性骨骼发育不良胎儿的流产皮肤组织及其父母的静脉血,通过提取基因组DNA,应用全外显子组测序技术对胎儿组织及其父母进行平行测序,对疑似致病变异用Sanger测序进行验证;并对以往报道基因检测结果进行文献复习,结合文献复习探讨该病的临床特点。结果发现了胎儿TRIP11检测到复合杂合变异c.790C>T(p.R264*)/c.589-2A>G,两位点分别来自父亲和母亲,符合常染色体隐性遗传。本研究报道的TRIP11基因复合杂合变异尚未见报道,拓宽了软骨生成不全1A型的基因变异谱,为该家庭的遗传咨询及产前诊断提供了重要的分子基础。

关键词：软骨生成不全IA型牙软骨发育不良甲状腺激素受体相互作用物11基因高尔基体微管相关蛋白210

分阶段体位干预对椎管内分娩镇痛初产妇的影响

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四川解剖学杂志 2024年第4期32卷 140-142页

作者：陈惠芳廖杨芬聂招兰赣州市赣县区人民医院妇产科赣州341100

目的:探讨分阶段体位干预对椎管内分娩镇痛初产妇分娩信心、产程进展和母乳喂养率的影响.方法:选取2020年8月至2023年8月本院收治的70例椎管内分娩镇痛初产妇为研究对象.根据干预方式,将其分为观察组(n=38,采用分阶段体位干预)和常规组(... 详细信息

目的:探讨分阶段体位干预对椎管内分娩镇痛初产妇分娩信心、产程进展和母乳喂养率的影响.方法:选取2020年8月至2023年8月本院收治的70例椎管内分娩镇痛初产妇为研究对象.根据干预方式,将其分为观察组(n=38,采用分阶段体位干预)和常规组(n=32,采用常规分娩干预).比较两组初产妇的分娩信心、分娩疼痛程度、产程进展以及泌乳时间和母乳喂养率.结果:干预后,观察组初产妇的简化中文版分娩自我效能量表(CBSEI-C32)评分高于常规组,视觉模拟评分法(VAS)评分低于常规组,差异均有统计学意义(P0.05).结论:通过采用分阶段体位干预手段对分娩镇痛初产妇进行管理,可有效提升产妇的分娩信心,减轻产妇生产过程中的疼痛感,加快生产进程,提高产后的母乳喂养率.

关键词：分阶段体位干预椎管内分娩镇痛初产妇分娩信心产程进展母乳喂养率

Recombinant fibronectin polypeptide antagonizes hepatic failure induced by endotoxin in mice

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Acta Pharmacologica Sinica 2004年第6期25卷 81-86页

作者： Yong WU, Yuan-zhong chen2, hui-fang HUANG, Ping chen Fujian Institute of Hematology, Union Hospital, Fujian Medical University, Fuzhou 350001,China Fujian Institute of Hematology Union Hospital Fujian Medical University Fuzhou 350001

AIM: To study the preventive effect of recombinant human fibronectin (rhFN) polypeptide on hepatic failure in- duced by endotoxin in mice. METHODS: A cDNA fragment coding for Ile1363-Tyr1725 of human FN was inserted into the PinPoint Xa-3 plasmid, and the constructed plasmid was transformed into E coli BL21 (DE3) cells, and then the expression of rhFN polypeptide in DE3 cells was identified through SDS-PAGE. The target protein from the supernatant of bacteria lysate was purified through biotin-affinity chromatography. The bioactivity of the purified rhFN polypeptide was determined with cell adhesiveactivity. The survival rate was observed in endotoxemia mice injected with rhFN polypeptide. The tissue damage in hepatocyte was detected using histology, ultrastructure, and DNA fragmentation assay. RESULTS: The expression of rhFN polypeptide reached approximately 20 % of the total cellular protein. The adhesion ability of rhFN polypeptide was concentration-dependent. The value of EC50 was 0.8 nmol/L. The survival rate of endotoxemia mice sensitized by D-galactosamine (D-GalN) was 60 % in rhFN polypeptide treated group, while that of endotoxemia mice sensitized by D-GalN was 20 % in the control group (P<0.01). Histopathology showed that less necrosis occurred on the hepatocyte of endotoxemia mice in- jected with rhFN polypeptide compared with saline control. Ultrastructure and DNA fragmentation assay showed that no apoptotic hepatocyte was observed in the liver of rhFN-treated endotoxemia mice. CONCLUSION: Re- combinant fibronectin polypeptide antagonizes hepatic failure induced by endotoxin in mice.

关键词： fibronectins recombinant fusion proteins lipopolysaccharides acetylgalactosamine

基于谱-效关系筛选菊茎叶总黄酮抗氧化物质基础及其机制探讨

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天然产物研究与开发 2024年第3期36卷 464-477页

作者：巫梦莹陈慧芳程冉冉丁杨飞熊俊伟夏成凯吴德玲张伟安徽中医药大学药学院合肥230012 安庆医药高等专科学校安庆246052 亳州职业技术学院安徽中药材种植联合研究中心亳州236800 安徽中医药大学国家中医药管理局中药炮制技术传承基地省部共建安徽道地中药材品质提升协同创新中心中药饮片制造新技术安徽省重点实验室合肥230012

为探讨菊茎叶总黄酮(total flavonoids from stems and leaves of Chrysanthemum morifolium,TFCSL)抗氧化应激的活性成分,阐明其药效物质基础和作用机制。采用HPLC建立不同批次TFCSL指纹图谱;以高浓度葡萄糖诱导人脐静脉内皮细胞建立... 详细信息

为探讨菊茎叶总黄酮(total flavonoids from stems and leaves of Chrysanthemum morifolium,TFCSL)抗氧化应激的活性成分,阐明其药效物质基础和作用机制。采用HPLC建立不同批次TFCSL指纹图谱;以高浓度葡萄糖诱导人脐静脉内皮细胞建立氧化损伤模型,将丙二醛含量、乳酸脱氢酶含量和超氧化物歧化酶活性作为药效指标;采用灰色关联度和偏最小二乘法分析其谱-效关系确定抗氧化药效物质基础;基于网络药理学结合分子对接探究核心靶点及作用通路。从12批次TFCSL指纹图谱中确定12个共有峰,指认其中9个化学成分;各批次总黄酮样品均可减少细胞凋亡、降低丙二醛及乳酸脱氢酶含量、提高超氧化物歧化酶活性;综合2种数学模型确定峰5(芹菜素-7-O-β-D-葡萄糖苷)、峰6(异绿原酸C)、峰7(香叶木素-7-O-β-D-葡萄糖苷)为抗氧化物质基础;筛选出的3个活性成分作用于抗氧化应激的33个靶点;关键靶点为TNF、CASP3、EDNRA、XDH、PTGS2、MMP2,主要涉及脂质和动脉粥样硬化信号通路、IL-17信号通路、糖尿病并发症AGE-RAGE信号通路、TNF通路、MPKA通路等信号通路;分子对接结果显示活性成分与关键靶点之间均有较好的结合力。表明TFCSL抗氧化应激的物质基础可能为芹菜素-7-O-β-D-葡萄糖苷、异绿原酸C、香叶木素-7-O-β-D-葡萄糖苷,推测通过TNF、CASP3等靶点作用动脉粥样硬化信号通路、IL17信号通路发挥作用,体现菊茎叶多成分、多靶点抗氧化的作用特点。

关键词：菊茎叶总黄酮氧化应激谱效关系

Overcoming the Na-ion conductivity bottleneck for the cost-competitive chloride solid electrolytes

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Journal of Energy Chemistry 2024年第8期95卷 1-8,I0001页

作者： Lv Hu hui Li fang chen Yating Liu Jinzhu Wang cheng Ma Hefei National Research Center for Physical Sciences at the Microscale CAS Key Laboratory of Materials for Energy ConversionDepartment of Materials Science and EngineeringUniversity of Science and Technology of ChinaHefei 230026AnhuiChina National Synchrotron Radiation Laboratory Hefei 230026AnhuiChina

Chloride solid electrolytes possess multiple advantages for the construction of safe,energy-dense allsolid-state sodium batteries,but presently the chlorides with sufficiently high cost-competitiveness for commercialization almost all exhibit low Na-ion conductivities of around 10^(-5)S cm^(-1)or ***,we report a chloride solid electrolyte,Na_(2.7)ZFCl_(5.3)O_(0.7),which reaches a Na-ion conductivity of 2.29×10^(-4)S cm^(-1)at 25℃without involving overly expensive raw materials such as rare-earth chlorides or Na_(2)*** addition to the efficient ion transport,Na_(2.7)ZrCl_(5.3)O_(0.7)also shows an excellent deformability surpassing that of the widely studied Na_(3)PS_(4),Na_(3)SbS_(4),and Na_(2)ZrCl_(6)solid *** combination of these advantages allows the all-solid-state cell based on Na_(2.7)ZrCl_(5.3)O_(0.7)and NaCrO_(2)to realize stable room-temperature cycling at a much higher specific current than those based on other non-viscoelastic chloride solid electrolytes in literature(120 mA g^(-1)vs.12-55 mA g^(-1));after 100 cycles at such a high rate,the Na_(2.7)ZFCl_(5.3)O_(0.7)-based cell can still deliver a discharge capacity of 80 mAh g^(-1)at25℃.

关键词： All-solid-state sodium batteries Ionic conductivities Solid electrolytes Chlorides High voltage stability

Privacy-Preserving Healthcare and Medical Data Collaboration Service System Based on Blockchain and Federated Learning

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Computers, Materials & Continua 2024年第8期80卷 2897-2915页

作者： fang Hu Siyi Qiu Xiaolian Yang ChaoleiWu Miguel Baptista Nunes hui chen College of Information Engineering Hubei University of Chinese MedicineWuhan430065China College of Information Engineering Zhongnan University of Economics and LawWuhan430073China School of Advanced Technologies Xi’an Jiaotong-Liverpool UniversitySuzhou215400China School of Information Management Central China Normal UniversityWuhan430079China

As the volume of healthcare and medical data increases from diverse sources,real-world scenarios involving data sharing and collaboration have certain challenges,including the risk of privacy leakage,difficulty in data fusion,low reliability of data storage,low effectiveness of data sharing,*** guarantee the service quality of data collaboration,this paper presents a privacy-preserving Healthcare and Medical Data Collaboration Service System combining Blockchain with Federated Learning,termed *** system is composed of three layers:Data extraction and storage,data management,and data *** on healthcare and medical data,a healthcare and medical blockchain is constructed to realize data storage,transfer,processing,and access with security,real-time,reliability,and *** improved master node selection consensus mechanism is presented to detect and prevent dishonest behavior,ensuring the overall reliability and trustworthiness of the collaborative model training ***,healthcare and medical data collaboration services in real-world scenarios have been discussed and *** further validate the performance of FL-HMChain,a Convolutional Neural Network-based Federated Learning(FL-CNN-HMChain)model is investigated for medical image *** model achieves better performance compared to the baseline Convolutional Neural Network(CNN),having an average improvement of 4.7%on Area Under Curve(AUC)and 7%on Accuracy(ACC),***,the probability of privacy leakage can be effectively reduced by the blockchain-based parameter transfer mechanism in federated learning between local and global models.

关键词： Blockchain technique federated learning healthcare and medical data collaboration service privacy preservation

Prognostic Biomarkers for Hepatocellular Carcinoma Based on Serine and Glycine Metabolism-related Genes

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Journal of Clinical and Translational Hepatology 2024年第3期12卷 266-277页

作者： Xufan Cai fang Xu Zhaohong Wang hui chen Shengzhang Lin Department of Clinical Medicine School of MedicineHangzhou City UniversityHangzhouZhejiangChina Zhejiang Chinese Medical University HangzhouZhejiangChina The First Affiliated Hospital of Bengbu Medical College BengbuAnhuiChina Department of Hepatobiliary and Pancreatic Surgery The Second Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiangChina

Background and Aims:Targeted therapy and immunotherapy have emerged as treatment options for hepatocellular carcinoma(HCC)in recent *** significance of serine and glycine metabolism in various cancers is widely *** study aims to investigate their correlation with the prognosis and tumor immune microenvironment(TIME)of ***:Based on the public database,different subtypes were identified by cluster analysis,and the prognostic model was constructed through regression *** gene expression omnibus(GEO)data set was used as the validation set to verify the performance of the *** survival curve evaluated prognostic *** was used to evaluate the level of immune cell infiltration,and maftools analyzed the *** screened small molecule compounds related to prognostic ***:HCC was found to have two distinct ***,we constructed a risk score prognostic model through regression analysis based on serine and glycine metabolismrelated genes(SGMGs).A nomogram was constructed based on risk scores and other clinical *** patients with a higher risk score showed a poor prognosis,and there were significant differences in immune cell infiltration between the high-and low-risk *** addition,three potential drugs associated with prognostic genes,streptozocin,norfloxacin,and hydrocotarnine,were ***:This study investigated the expression patterns of SGMGs and their relationship with tumor characteristics,resulting in the development of a novel model for predicting the prognosis of HCC *** study provides a reference for clinical prognosis prediction and treatment of HCC patients.

关键词： Serine and glycine metabolism-related genes Hepatocellular carcinoma Risk group Prognosis Tumor immune microenvironment

文冠果油神经酸乙酯对脱髓鞘小鼠脂肪酸、血脂的影响

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食品安全质量检测学报 2024年第2期15卷 235-242页

作者：陈方会杜俊民张芳荣贺文彬李钦青李春江胡美变山西中医药大学中药与食品工程学院晋中030619 山西中医药大学中医脑病山西省重点实验室晋中030619

目的探究文冠果油神经酸乙酯对双环己酮草酰二腙(cuprizone,CPZ)诱导的脱髓鞘小鼠脂肪酸、血脂的影响。方法SPF级雌性昆明小鼠50只,除空白组(KO)外,通过在饲料中加入0.25%CPZ饲喂雌性昆明小鼠造模并分为模型组(CPZ)、神经酸乙酯低剂量组... 详细信息

目的探究文冠果油神经酸乙酯对双环己酮草酰二腙(cuprizone,CPZ)诱导的脱髓鞘小鼠脂肪酸、血脂的影响。方法SPF级雌性昆明小鼠50只,除空白组(KO)外,通过在饲料中加入0.25%CPZ饲喂雌性昆明小鼠造模并分为模型组(CPZ)、神经酸乙酯低剂量组(NA-L,0.001912 mL/g)、神经酸乙酯中剂量组(NA-M,0.003824 mL/g)、神经酸乙酯高剂量组(NA-H,0.007648 mL/g),持续5周至实验结束。于造模2周后开始给药至第5周结束后取材,并通过气相色谱仪、血脂仪分别检测文冠果油神经酸乙酯对CPZ诱导的脱髓鞘小鼠脑组织、肝脏、心脏、血清、粪便里的脂肪酸含量及血液中血脂水平的影响。结果与KO组相比,CPZ组脱髓鞘小鼠脑组织、肝脏、心脏中神经酸、芥酸含量显著下降(P<0.05);与CPZ组相比,NA-L、NA-M、NA-H组小鼠脑组织、肝脏、心脏、血清、粪便中的神经酸含量均显著增加(P<0.05),其中KO、CPZ组小鼠血清、粪便中未检测到神经酸和芥酸,NA-L、NA-M、NA-H组小鼠血清、粪便中含有一定量的神经酸和芥酸;与KO组相比,CPZ组血脂升高;与CPZ组相比,NA-L、NA-M、NA-H组均可降低血液中的甘油三酯(triglyceride,TG)含量,升高高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)含量。结论在饲料中添加0.25%CPZ诱导小鼠脱髓鞘会导致小鼠血脂升高,降低小鼠体内神经酸、芥酸含量,脱髓鞘疾病与神经酸的积累有关,脱髓鞘疾病会导致神经酸的供应受损。在这种情况下,文冠果油神经酸乙酯提取物能够增加脱髓鞘小鼠体内神经酸及芥酸含量,提高脑组织不饱和脂肪酸含量,调节脱髓鞘小鼠血脂水平。此外,神经酸、芥酸参与脱髓鞘小鼠体内代谢,并在小鼠体内共存,文冠果油神经酸乙酯提取物可作为脱髓鞘小鼠补充神经酸的外源性物质。

关键词：文冠果油神经酸乙酯芥酸脂肪酸脱髓鞘

Uniqueness on Difference Operators of Meromorphic Functions of Infinite Order

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Acta Mathematica Sinica,English Series 2024年第2期40卷 511-527页

作者： hui LI Ming Liang fang Xiao YAO School of Science China University of Mining and TechnologyBeijing 100083P.R.China Institute of Mathematics Academy of Mathematics and System SciencesChinese Academy of SciencesBeijing 100190P.R.China School of Sciences Hangzhou Dianzi UniversityHangzhou 310012P.R.China School of Mathematical sciences and LPMC Nankai UniversityTianjin 300071P.R.China

We investigate the uniqueness problems of meromorphic functions and their difference operators by using a new *** is proved that if a non-constant meromorphic function f shares a non-zero constant and ∞ counting multiplicities with its difference operators Δcf(z) and Δ_(c)^(2)f(z), thenΔcf(z)≡Δ_(c)^(2)f(z).In particular,we give a difference analogue of a result of *** method has two distinct features:(ⅰ) It converts the relations between functions into the corresponding *** makes it possible to deal with the uniqueness problem by linear algebra and combinatorics.(ⅱ) It circumvents the obstacle of the difference logarithmic derivative lemma for meromorphic functions of infinite order,since this method does not depend on the growth of the ***,the idea in this paper can also be applied to the case for several variables.

关键词： Uniqueness meromorphic functions difference operators infinite order