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Apple stem grooving virus is associated with leaf yellow mottle mosaic disease on Citrus grandis cv. Huangjinmiyou in China

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Journal of Integrative Agriculture 2022年第7期21卷 2031-2041页

作者： XUAN Zhi-you ZHANG Song LI Ping YANG Fang-yun chen hong-ming LIU Ke-hong ZHOU Yan LI Zhong-an ZHOU Chang-yong CAO Meng-ji National Citrus Engineering and Technology Research Center Citrus Research InstituteSouthwest UniversityChongqing 400712P.R.China State Cultivation Base of Crop Stress Biology for Southern Mountainous Land Academy of Agricultural SciencesSouthwest UniversityChongqing 400715P.R.China

Although it is usually latent on citrus, apple, and pear, apple stem grooving virus(ASGV) poses a great risk to many sensitive cultivars. Since severe leaf yellow mottle mosaic(LYMM) symptoms have been observed on Huangjinmiyou(HJY) pummelos(Citrus grandis cv. Huangjinmiyou), a commercial variety that is widely cultivated in South China, high throughput sequencing(HTS) was used to find potential pathogens and only three divergent ASGV variants were identified. The three ASGV variants shared 81.03–82.34% genome-wide pairwise identities with each other, and were separately closest to other ASGV variants from different hosts and/or geographical regions, as indicated by viral phylogenies. However, these new variants may have developed from viral interstrain interactions, based on the results of recombination analysis. A large-scale survey using reverse transcription-PCR(RT-PCR) protocols designed for the three ASGV variants revealed a high incidence(92.7–100%) of ASGV in symptomatic HJY trees from 11 major citrusproducing regions in China. None of ASGV were detected in asymptomatic trees. Temperature treatments applied to the symptomatic HJY plants showed that ASGV is sensitive to high temperatures(30–35°C), at which not only the plants recovered, but also the viruses were not detected by RT-PCR, while at low temperatures(20–24°C), both the symptoms and viruses remained detectable. These data show that ASGV is associated with the LYMM disease prevalent on HJY in China, and this is the significant basis especially of taking appropriate measures timely to manage the disease.

关键词： citrus pummelo apple stem grooving virus genome sequence thermotherapy

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权衡生态和发电目标的梯级水库强化学习模型及其应用

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水电能源科学 2024年第2期42卷 188-191,206页

作者：王一聪陈明洪中国农业大学水利与土木工程学院北京100083

为更好地发挥经济及生态效益,建立了基于强化学习算法的梯级水库优化调度模型,以周尺度小浪底天然入库流量过程为基础,探讨梯级水库发电与生态目标之间的权衡,并将该模型应用于小浪底-西霞院梯级水库中,分别探讨了不同调度方案下发电最... 详细信息

为更好地发挥经济及生态效益,建立了基于强化学习算法的梯级水库优化调度模型,以周尺度小浪底天然入库流量过程为基础,探讨梯级水库发电与生态目标之间的权衡,并将该模型应用于小浪底-西霞院梯级水库中,分别探讨了不同调度方案下发电最优、生态最优和发电-生态权衡的优化调度策略。结果表明,以发电最优为目标时,梯级水库多年平均发电量比常规调度增加了3.62%~7.92%;以生态最优为目标时,平均生态保证率比常规调度增加了31.68%~33.66%。结果为梯级水库多目标优化调度提供了一种可行方法。

关键词：多目标调度生态保证率发电量强化学习小浪底水库西霞院水库

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Survey and examination of the potential alternative hosts of Villosiclava virens, the pathogen of rice false smut, in China

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Journal of Integrative Agriculture 2015年第7期14卷 1332-1337页

作者： DENG Qi-de YONG ming-li LI Dan-yang LAI Chao-hui chen hong-ming FAN Jing HU Dong-wei The State Key Laboratory of Rice Biology Biotechnology Institute Zhejiang University Plant Protection Station of Xiangshan County Rice Research Institute Sichuan Agricultural University

Rice false smut is caused by ascomycete Villosiclava virens, whose potential alternative hosts have been assumed previ- ously. Here its potential alternative hosts were surveyed and identified from 2008 to 2013 in the main rice-growing regions in China. Two common weeds in paddy fields, Digitaria sanguinalis Scop. and Echinochloa crusgalli （L.） Beauv., were found to present the similar symptoms to smut diseases in a few individuals in 2012 and 2013 in Zhejiang and Sichuan provinces of China, respectively. After the examinations of the spore morphology, their infection and extension mode in hosts, pathogen cell wall components, and molecular identification, the two pathogens were identified to be the Basidiomycetes, Ustilago syntherismae and Ustilago trichophora, respectively. So far there has been no alternative host of V. virens to be identified in China. These suggest that the alternative hosts of V. virens, if they do exist, are not possible to play an important role in the pathogen life cycle and the disease epidemics.

关键词： alternative host Villosiclava virens Digitaria sanguinalis Scop. Echinochloa crusgalli （L.） Beauv. Ustilagospp. rice false smut

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The complete genome sequence of Citrus vein enation virus from China

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Journal of Integrative Agriculture 2015年第3期14卷 598-601页

作者： HUANG Ai-jun SONG Zhen CAO Meng-ji chen hong-ming LI Zhong-an ZHOU Chang-yong Citrus Research Institute Chinese Academy of Agricultural Sciences Southwest University College of Plant Protection Southwest University

The complete nucleotide sequence of an isolate of Citrus vein enation virus（CVEV-XZG） from China has been determined for the first time. The genome consisted of 5 983 nucleotides, coding for five open reading frames（ORFs）, had a similar genomic organization features with Pea enation mosaic virus（PEMV）. Nucleotide and deduced amino acid sequence identity of the five ORFs compared to isolate CVEV VE-1 range from 97.1 to 99.0% and 97.4 to 100.0%, these values compared to isolate PEMV-1 range from 45.2 to 51.6% and 31.1 to 45.2%. Phylogenetic analysis based on the complete genome sequence showed that the isolate CVEV-XZG had close relationship with Pea enation mosaic virus. The results supports CVEV may be a new member of genus Enamovirus. The full sequence of CVEV-XZG presented here may serve as a basis for future study of CVEV in China.

关键词： Citrus vein enation virus（CVEV）,genome sequence,Enamovirus

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Effect of temperature on the development of sclerotia in Villosiclava virens

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Journal of Integrative Agriculture 2016年第11期15卷 2550-2555页

作者： FAN Lin-lin YONG ming-li LI Dan-yang LIU Yi-jia LAI Chao-hui chen hong-ming chenG Fang-min HU Dong-wei The State Key Laboratory of Rice Biology Biotechnology InstituteZhejiang UniversityHangzhou 310058P.R.China Plant Protection Station of Xiangshan County Xiangshan 315700P.R.China Crop Science Institute Zhejiang UniversityHangzhou 310058P.R.China

The sclerotia of Villosiclava virens were found commonly in high altitude and the temperate regions, where the temperatures are relatively low in rice filling stage. To make sure if low temperature induce the sclerotial formation in V. virens, the inoculated rice panicles in laboratory and the diseased rice panicles cut from paddy fields were treated under different temperatures. The results showed that 3 days of night temperature at 15°C were enough to induce the sclerotial formation. The low temperature was much more effective for young balls with intact membranes. After appearance of chlamydospores on the ball surfaces, the sclerotium could not differentiate anymore. The sclerotia began to differentiate below the chlamydospore layer and gradually grew onto the ball surfaces. This suggests that low temperature in the early development stage of false smut balls is an important factor to induce the sclerotial differentiation, and rice cultivars with long growth periods are able to produce more sclerotium-bearing balls, which will produce mass of spores in paddy field in the coming year.

关键词： Villosiclava virens Ustilaginoidea virens rice false smut sclerotial formation temperature

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An Insect Imaging System to Automate Rice Light-Trap Pest Identification

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Journal of Integrative Agriculture 2012年第6期11卷 978-985页

作者： YAO Qing LV Jun LIU Qing-jie DIAO Guang-qiang YANG Bao-jun chen hong-ming TANGJian College ofInformatics and Electronics Zhejiang Sci-Tech University Hangzhou 310018 P.R.China State Key Laboratory of Rice Biology China National Rice Research Institute Hangzhou 310006 P.R.China Xiangshan Agriculture and Forestry Bureau Ningbo 315700 P.R. China

Identification and counting of rice light-trap pests are important to monitor rice pest population dynamics and make pest forecast. Identification and counting of rice light-trap pests manually is time-consuming, and leads to fatigue and an increase in the error rate. A rice light-trap insect imaging system is developed to automate rice pest identification. This system can capture the top and bottom images of each insect by two cameras to obtain more image features. A method is proposed for removing the background by color difference of two images with pests and non-pests. 156 features including color, shape and texture features of each pest are extracted into an support vector machine （SVM） classifier with radial basis kernel function. The seven-fold cross-validation is used to improve the accurate rate of pest identification. Four species of Lepidoptera rice pests are tested and achieved 97.5% average accurate rate.

关键词： automatic identification imaging system rice light-trap pests SVM cross-validate

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浅析动漫元素在商品包装中的应用

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绿色包装 2024年第2期 75-77页

作者：陈明红邹苇张滟妮张靖四川音乐学院成都美术学院四川成都610000

近年来,我国的经济文化发展速度和成就令世人瞩目,人们的生活水平持续提升,越来越注重生活品质,对商品包装从单纯满足实用性发展到追求观赏性和舒适性。当下,带有动漫元素的商品包装逐渐受到年轻一代的关注和喜爱。本文对动漫元素在包... 详细信息

近年来,我国的经济文化发展速度和成就令世人瞩目,人们的生活水平持续提升,越来越注重生活品质,对商品包装从单纯满足实用性发展到追求观赏性和舒适性。当下,带有动漫元素的商品包装逐渐受到年轻一代的关注和喜爱。本文对动漫元素在包装中的应用进行研究,分析其能产生的巨大社会价值及经济价值。

关键词：动漫元素经济消费包装设计

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Engineered extracellular vesicles: A new approach for targeted therapy of tumors and overcoming drug resistance

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Cancer Communications 2024年第2期44卷 205-225页

作者： chen ming-Kun chen Zi-Xian Cai Mao-Ping chen hong chen Zhuang-Fei Zhao Shan-Chao Department of Urology The Third Affiliated Hospital of Southern Medical UniversityGuangzhouGuangdongP.R.China The Third Clinical College Southern Medical UniversityGuangzhouGuangdongP.R.China Luoyang Key Laboratory of Organic Functional Molecules College of Food and DrugLuoyang Normal UniversityLuoyangHenanP.R.China Department of Urology Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdongP.R.China

Targeted delivery of anti-tumor drugs and overcoming drug resistance in malig-nant tumor cells remain significant clinical challenges.However,there are only few effective methods to address these issues.Extracellular vesicles(EVs),actively secreted by cells,play a crucial role in intercellular information transmis-sion and cargo transportation.Recent studies have demonstrated that engineered EVs can serve as drug delivery carriers and showed promising application prospects.Nevertheless,there is an urgent need for further improvements in the isolation and purification of EVs,surface modification techniques,drug assem-bly processes,and precise recognition of tumor cells for targeted drug delivery purposes.In this review,we summarize the applications of engineered EVs in cancer treatment and overcoming drug resistance,and current challenges asso-ciated with engineered EVs are also discussed.This review aims to provide new insights and potential directions for utilizing engineered EVs as targeted delivery systems for anti-tumor drugs and overcoming drug resistance in the near future.

关键词： drug delivery drug resistance extracellular vesicles nanoparticles

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Near real-time gravitational wave data analysis of the massive black hole binary with TianQin

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Science China(Physics,Mechanics & Astronomy) 2024年第7期67卷 175-186页

作者： hong-Yu chen Xiang-Yu Lyu En-Kun Li Yi-ming Hu MOE Key Laboratory of TianQin Mission TianQin Research Center for Gravitational Physics&School of Physics and AstronomyFrontiers Science Center for TianQinGravitational Wave Research Center of CNSASun Yat-sen University(Zhuhai Campus)Zhuhai 519082China

Space-borne gravitational wave(GW)detectors can detect the merger of massive black holes.The early warning and localization of GW events before merging can be used to inform electromagnetic telescopes and conduct multimessenger observations.However,this requires real-time data transmission and analysis capabilities.The geocentric orbit of the space-borne GW detector Tian Qin makes it possible to conduct real-time data transmission.In this study,we develop a search and localization pipeline for massive black hole binaries(MBHBs)with Tian Qin under both regular and real-time data transmission modes.We demonstrate that,with real-time data transmission,MBHBs can be accurately localized on the fly.With the approaching merger,each analysis can be finished in only 40 min.For an MBHB system at a distance of 1 Gpc,if we receive data every hour,then we can pinpoint its location to within less than 1 deg^(2)on the final day before the merger.

关键词： gravitational wave detectors and experiments black holes data analysis algorithms and implementation data management

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A Review of Type 1 and Type 2 Intraductal Papillary Neoplasms of the Bile Duct

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Current Medical Science 2024年第3期44卷 485-493页

作者： Xia-hui HUANG Tian-xiang chen hong-liang LIU ming-wen HUANG Department of General Surgery The Second Affiliated Hospital of Nanchang UniversityNanchang330006China

Intraductal papillary neoplasm of the bile duct(IPNB)is a heterogeneous disease similar to intraductal papillary mucinous neoplasm of the pancreas.These lesions have been recognized as one of the three major precancerous lesions in the biliary tract since 2010.In 2018,Japanese and Korean pathologists reached a consensus,classifying IPNBs into type l and type 2 IPNBs.IPNBs are more prevalent in male patients in East Asia and are closely related to diseases such as cholelithiasis and schistosomiasis.From a molecular genetic perspective,IPNBs exhibit early genetic variations,and different molecular pathways may be involved in the tumorigenesis of type 1 and type 2 IPNBs.The histological subtypes of IPNBs include gastric,intestinal,pancreaticobiliary,or oncocytic subtypes,but type 1 IPNBs typically exhibit more regular and well-organized histological features than type 2 IPNBs and are more commonly found in the intrahepatic bile ducts with abundant mucin.Due to the rarity of these lesions and the absence of specific clinical and laboratory features,imaging is crucial for the preoperative diagnosis of IPNB,with local bile duct dilation and growth along the bile ducts being the main imaging features.Surgical resection remains the optimal treatment for IPNBs,but negative bile duct margins and the removal of lymph nodes in the hepatic hilum significantly improve the postoperative survival rates for patients with IPNBs.

关键词： intraductal papillary neoplasm of the bile duct subclassification neoplasms precancerous tissue prognosis

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