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Polypeptide from Chlamys farreri attenuates murine thymocytes damage induced by ultraviolet B

Acta Pharmacologica Sinica 2007年第10期28卷 1665-1670页

作者： Hai-ying chen~(2.3),Xiao CHU~4,Chun-ling YAN~5,Xue-hong chen~5,Mi SUN~6,Yue-jun WANG~6,Chun-bo WANG~(3,7),Wen-gong YU~(2.7)~2.Department of Pharmacochemistry,{2. university of China,qingdao 2.6003,China ~3 Department of Pharmacology,Medical college,qingdaouniversity,qingdao 2.602.,China ~4 {2. Municipal Hospital,qingdao 2.6010,China ~5 Department of Pathophysiology,Medicalcollege,qingdao university,qingdao 2.602.,China ~6 Yellow Sea fishery Research Institute,qingdao 2.6071,China Department of Pharmacochemistry Ocean University of China Qingdao China Department of Pharmacology Medical College Qingdao University Qingdao China qingdao Municipal Hospital Qingdao China Department of Pathophysiology Medical College Qingdao University Qingdao China Yellow Sea fishery Research Institute Qingdao China

Aim:Polypeptide from Chlamys farreri (PCF,molecular mass is 879) is a newmarine polypeptide compound isolated from Chlamys *** study investi-gates the possible protective roles and the mechanism of PCF against ultravioletB (UVB)-induced apoptosis in murine ***:The rate of apoptosisand caspase-3 activation was measured by flow *** expression ofstress-response genes c-fos and c-jun was observed by *** blotanalysis was performed to determine the release of cytochrome ***:It wasfound that UVB induced murine thymocyte *** cells treated with UVBshowed an increase in cytochrome c release,caspase-3 activity,as well as in theexpression of c-fos and *** addition,all were involved in UVB-induced ***:Our present observations pointed to the ability of PCF toavert UVB-induced apoptosis in thymocytes by modulating c-fos and c-junexpression,cytochrome c release,and the consequent activation of caspase-3,which were essential components of the UV-induced cell apoptotic *** suggested that PCF is a promising protective substance against UVradiation.

关键词： polypeptide from Chlamys farreri (PCF) ultraviolet B apoptosis cytochrome c caspase-3

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Numerical study of current fields near the Changjiang estuary and impact of Quick-EnKF assimilation

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Acta oceanologica Sinica 2011年第5期30卷 33-44页

作者： chen Xueen ZHAN Peng chen Jinrui QIAN Hongbao college of Physical and Environmental oceanography Ocean University of China Qingdao 266100 China Administrative Center for China＇s Agenda 2. Beijing 100038 China

A 30-d current numerical simulation is running for the Yangshan Port,the Changjiang estuary,the Hangzhou Bay and their adjacent seas using a finite volume coastal ocean model （FVCOM）,with Changjiang River runoff and wind effect being *** the open boundary,this model is driven by the water level obtained from prediction including eight main partial *** the harmonic analysis,the cotidal chart and the iso-amplitude line as well as the current ellipse distribution map are displayed to illustrate the propagation property of a tidal *** velocity of both the U and V components coincides with the actual measurement,which shows that the model result is credible to describe the hydrodynamic pattern in this sea *** this basis,real-time current data from high-frequency radar is assimilated with the implementation of quick ensemble Kalman filter,which takes the variation tendency of the state vector to compute the analysis field,instead of integrating the field for N （the number of ensemble） times as it used to in the standard EnKF,aiming at raising the efficiency of computation,reducing the error of prediction and at the same time,improving the forecast effect.

关键词： Yangshan Port finite volume coastal ocean model current quick ensemble Kalman filter assimilation

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SENSITIVITY STUDY OF THE EFFECTS OF WAVE-INDUCED VERTICAL MIXING ON VERTICAL EXCHANGE PROCesSes

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Journal of Hydrodynamics 2010年第3期22卷 410-418页

作者： LIANG Bing-chen LEE Dong-yong LI Hua-jun ZHANG Jing Key Laboratory of ocean Engineering Shandong Province Ocean University of China Qingdao 266100 China Coastal and Harbour Engineering Research Center Korea Ocean Research and Development Institute AnsanKorea college of Engineering Ocean University of China Qingdao 266100 China

The hydrodynamic model COHERENS-SED, developed by the present authors through introducing wave-enhanced bottom stress, wave dependent surface drag coefficient, wave-induced surface mixing, SWAN to COHERENS, is modified to account for wave-induced vertical mixing. The COHERENS-SED model can also be used for one-dimensional, two-dimensional, three-dimensional current and salinity calculations. One-dimensional model and three-dimensional model are used to study the effects of the wave-induced vertical mixing. The horizontal current velocity profiles obtained by the model are in good agreement with the analytical velocity profiles under the same input conditions. Numerical results show that higher wave height would generally generate larger vertical eddy viscosity and lower horizontal velocity. The results for fresh water in Yellow River Delta show that the wave-induced vertical mixing increases the momentum of fresh water transferring ability downwards to seabed and salt water＇s mixing with upper fresh water. Fresh water flume length is compressed considerably.

关键词： Yellow River Delta COHERENS SWAN COHERENS-SED wave-induced vertical mixing

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Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis)

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Acta oceanologica Sinica 2010年第2期29卷 81-87页

作者： SHA Zhenxia REN Guocheng WANG Xianli WANG Na chen Songlin Key Laboratory of Sustainable Development of Marine fisher.es Ministry of Agriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fisheries Sciences Qingdao 266071 China college of Biology Shandong Normal University Jinan 250014 China college of Marine Life Ocean University of China Qingdao 266003 China

A new cell line, CSEC, has been successfully established from embryos at gastrula stage of a cultured marine fish, half smooth tongue sole （Cynoglossus sernilaevis）. CSEC cells grow actively and stably more than 50 passages for over 2.0 d in DMEM medium supplemented with 15% FBS （fetal bovine serum）, 2.5 ng/cm^3 bFGF （basic fibroblast growth factor）, 1 ng/cm^3 LIF （leukemia inhibitory factor） and 50 mmol/dm^3 2.ME （2.mecaptoethanol）. The cells grew well in the temperature range of 2.-30 ℃ and the optimal growth temperature was 2. ℃. FBS and bFGF concentrations are the two key components for CSEC cells proliferation. Chromosome analysis reveals that CSEC cells have a normal diploid karyotype with 2.=42.. The significant fluorescent signals were observed in CSEC after transfection with the GFP reporter gene, suggesting that the CSEC cell line can be used as a useful tool for transgenic and genetic manipulation studies. CSEC cells showed the cytopathic effect （CPE） after infection with lymphosystis disease virus （LCDV） in 2.d. Moreover, the LCDV particles can be observed in the cytoplasm of virus-infected cells by electron microscopy. It suggests that CSEC could be potentially used for the study of aquatic virus.

关键词： embryonic cell line half smooth tongue sole karyotype GFP LCDV CSEC

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垃圾衍生燃料等温快速热解和燃烧反应特性

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燃料化学学报 2004年第4期32卷 440-445页

作者：王志奇陈勇青岛科技大学化学与分子工程学院山东青岛266042 中国科学院广州能源研究所广东广州510070

利用热天平和管式炉对RDF(RefuseDerivedFuel)等温快速热解和燃烧反应特性进行了研究。实验发现,在等温快速升温的条件下,RDF热解和燃烧的反应速率都非常快,从受热开始到反应结束需60s～80s;从开始失重到完成反应为2.s。RDF热解和燃烧... 详细信息

利用热天平和管式炉对RDF(RefuseDerivedFuel)等温快速热解和燃烧反应特性进行了研究。实验发现,在等温快速升温的条件下,RDF热解和燃烧的反应速率都非常快,从受热开始到反应结束需60s～80s;从开始失重到完成反应为2.s。RDF热解和燃烧热重反应曲线非常类似,都只有一个反应失重区;RDF组成对其燃烧和热解反应性有重要影响,含有橡胶的RDF的热解和燃烧反应速率较小。在650℃～800℃RDF快速热解产物中气、液产物的产率可达80%～90%,而固体产物的产率只有10%～2.%,热解气体的热值为2.kJ/m3,RDF较适合进行热解处理。

关键词：垃圾衍生燃料等温快速热解等温快速燃烧热解产物

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On four closely related hypotrichous ciliates(Protozoa,Ciliophora,Spirotrichea):molecular characters,interspecific relationships and phylogeny defined with multigene sequence information

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Acta oceanologica Sinica 2010年第5期29卷 90-96页

作者： GAO Feng YI Zhenzhen chen Zigui AL-RASHEID Khaled A S SONG Weibo Laboratory of Protozoology Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education College of Life Science South China Normal University Guangzhou 510631 China Laboratory of Protozoology KLM Ocean University of China Qingdao 266003 China Zoology Department King Saud University Riyadh 11451 Saudi Arabia

In order to clarify the phylogeny and relationships of the most confused hypotrichous ciliates,Holosticha-complex,four closely related holostichids（five populations）,Holosticha bradburyae,***,Anteholosticha sp.,and ***,were compared and analyzed using ITS2.secondary structures,ITS1-5.8S-ITS2.region and SSrRNA gene *** ITS1-5.8S-ITS2.region sequences of these four species were first sequenced,and they shared sequence identities ranging from 68.0% to 90.1%,while two populations of Anteholosticha *** in three nucleotides（sequence identity 99.8%）.There were several minor differences among ITS2.secondary structures of these species,while two populations of Anteholosticha *** the identical secondary *** trees inferred from the ITS1-5.8S-ITS2.region sequences of stichotrichs using multiple algorithms（Neighbor-Joining,Maximum Parsimony and Bayesian） revealed similar *** results show that：（1） Holosticha bradburyae and *** firmly clustered together with strong bootstrap supports,forming a sister clade with Anteholosticha sp.,（2. Anteholosticha appeared to be a paraphyletic assemblage,in which the morphotype *** was more closely related to Diaxonella trimarginata than to its congener Anteholosticha *** analyses based on the SSrRNA gene and the combined sequences of SSrRNA gene and ITS1-5.8S-ITS2.region revealed the similar relationships between Holosticha and Anteholosticha,nevertheless their positions within the subclass Stichotrichia differed from each other inferred from different genes.

关键词： phylogeny gene sequencing marine ciliates SSrRNA ITS1-5.8S-ITS2

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BOTTOM SHEAR STResS UNDER WAVE-CURRENT INTERACTION

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Journal of Hydrodynamics 2008年第1期20卷 88-95页

作者： LIANG Bing-chen LI Hua-jun LEE Dong-yong college of Engineering Ocean University of China Qingdao 266100 China Coastal and Harbour Engineering Research Center Korea Ocean Research and Developmcnt Institute Ansan Korea

The present work adopts the COHERENS-SWAN model developed by the first author through coupling three-dimensional hydrodynamic model （COHERENS） and third-generation wave model （SWAN）. Inside the COHERENS-SWAN, the SWAN is regarded as a subroutine and the time- and space-varying current velocity and surface elevation are obtained from the COHERENS. Wave-enhanced bottom shear stress, wave induced surface mixing length and wave dependent surface drag coefficient have been introduced into the COHERENS. Secondly, as wave-enhanced bottom shear stress （＂bottom shear stress＂ described as BSS sometimes in this article） is concerned, a modified bottom shear stress Grant and Madsen model which introduces random wave field is given and introduced to COHERENS-SWAN. COHERENS-SWAN is also adopted to simulate three-dimensional flow in the Yellow River Delta with wave-current co-existing. Four numerical experiments were given to study the effects of wave-current interaction on enhancing bottom shear stress. The simulated current velocities, wave height and wave period match well with field measurement data. The simulated significant wave height and wave period for the case with considering the effects of current can give better agreement with measurement data than the case without involving the effects of current. The introduction of random wave generates lower the bottom shear stress than the case without introducing it. There are obvious differences between bottom shear stress of two way interaction and one way interaction. Velocity field obtained by the COHERENS-SWAN is reasonable according to previous studies and measurements.

关键词： Yellow River Delta COHERENS SWAN wave-current interaction bottom shear stress

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estimates of genetic and phenotypic parameters for weight and length in Paralichthys olivaceus(Temminck et Schlegel)

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Acta oceanologica Sinica 2011年第6期30卷 58-64页

作者： TIAN Yongsheng XU Tianjun LIANG You chen Songlin Key Laboratory for Sustainable Utilization of Marine fisher.es Resources Ministry of Agriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao 266071 China Laboratory for Marine Living Resources and Molecular Engineering College of Marine ScienceZhejiang Ocean University Zhoushan 316000 China

The complete diallel cross design was employed to estimate the genetic parameters of the growth of Japanese flounder （Paralichthys olivaceus）. A total of 60 full-sib families were cultivated and two growth-related traits, body weight （BW） and body length （BL）, were examined at average 2. 3 and 8 months of age respectively, with 1 800 individuals measured in each age group （30 per family）. Based on the additive-dominance-maternal-effect genetic analysis model, the restricted maximum likelihood approach was used to estimate various （co）variance components. The results showed that narrow-sense heritability estimates of BW and BL were respectively： 0.2. ± 0.01 and 0.2. ± 0.02.at 2.months of age, 0.32.± 0.02.and 0.30 ± 0.04 at 3 months of age, 0.48 ± 0.04 and 0.40 ± 0.05 at 8 months of age; broad-sense heritability estimates were respectively： 0.44 ± 0.02.and 0.54 ± 0.04 at 2.months of age, 0.35 ± 0.01 and 0.36 ± 0.03 at 3 months of age, 0.49 ± 0.03 and 0.45 ± 0.04. All heritabilities were statistically significant （P 0.05）. Additive genetic correlations between BW and BL at 2. 3 and 8 months of age were consistently positive and highly significant （P 0.01）： 0.93 ± 0.02. 0.95 ± 0.03 and 0.92.± 0.03 respectively. Maternal effect was significant （P 0.05） only at 2.months of age, and was not detected at 3 and 8 months of age. According to the heritability estimates, the mass selection strategy should be efficient for the breeding of Japanese flounder.

关键词： Paralichthys olivaceus heritability genetic correlation genetic mating design diallel cross maternal effect

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Numerical study of M_2.internal tide generation and propagation in the Luzon Strait

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Acta oceanologica Sinica 2011年第5期30卷 23-32页

作者： LI Huan SONG Dan chen Xueen QIAN Hongbao MU Lin SONG Jun National Marine Data and Information Service State Oceanic Administration Tianjin 300171 China Key Laboratory of ocean Circulation and Waves Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China college of Physical and Environmental oceanography Ocean University of China Qingdao 266100 China Administrative Center for China＇s Agenda 2. Beijing 100038 China

Based on the z-coordinate ocean model HAMSOM,we introduced the internal-tide viscosity term and applied the model to numerically investigate the M2.internal tide generation and propagation in the Luzon Strait （LS）.The results show that （1） in the upper 2.0 m depth,at the thermocline,the maximum amplitude of the generated internal tides in the LS can reach 40 m;（2. the major internal tides are generated to the northwest of Itbayat Island,the southwest of Batan Island and the northwest of the Babuyan Islands;（3） during the propagation the baroclinic energy scattering and reflection is obvious,which exists under the effect of the specific topography in the South China Sea （SCS）;（4） the westward-propagating internal tides are divided into two branches entering the *** passing through 118 E,the major branch is divided into two branches *** strongest internal tides in the LS are generated to the northwest of Itbayat Island and propagate northeastward to the ***,to the east of 12. E,most of the internal tides propagate southeastward to the Pacific as a beam.

关键词： M2.internal tides HAMSOM Internal tidal energy flux Luzon Strait

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A hybrid model for numerical wave forecasting and its implementation-Ⅰ.The wind wave model

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Acta oceanologica Sinica 1989年第1期8卷 1-14页

作者： Wen Shengchang (S.C. Wen)1, Zhang Dacuo, chen Bohai and Guo Peifang Institute of Physical oceanography, ocean {2. of qingdao (Formerly, Shandong college of oceanography), qingdao, China

The authors make an endeavor to explain why a new hybrid wave model is here proposed when several such models have already been in operation and the so- called third generation wave modej is proving attractive. This part of the paper is devoted to the wind wave model. Both deep and shallow water models have been developed, the former being actually a special case of the latter when water depth is great. The deep water model is exceptionally simple in form. Significant wave height is the only prognostic variable. In comparison with the usual methods to compute the energy input and dissipations empirically or by 'tuning', the proposed model has the merit that the effects of all source terms are combined into one term which is computed through empirical growth relations for significant waves, these relations being, relatively speaking, easier and more reliable to obtain than those for the source terms in the spectral energy balance equation. The discrete part of the model and the implementation of the model as a whole will be discussed in the second part of the present paper.

关键词： wave A hybrid model for numerical wave forecasting and its implementation The wind wave model

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