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Detection of Prorocentrum donghaiense using sandwich hybridization integrated with nuclease protection assay

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Acta oceanologica Sinica 2009年第2期28卷 121-126页

作者： chen Jie ZHEN Yu MI Tiezhu YU Zhigang college of Marine Life Science Ocean University of China Qingdao 266003 China college of Environmental Science and Engineering Ocean University of China Qingdao 266100 China college of Chemistry and Chemical Engineering Ocean University of China Qingdao 266100 China

Prorocentrum donghaiense is an important harmful algae bloom （HAB） causing creature in China＇s seas, and the conventional visual detection can not cope with long-term monitoring and highthroughput sampling projects. An assay for P. donghaiense with sandwich hybridization integrated with nuclease protection assay （NPA-SH） was established. Tests with mixed samples and spiked field ones confirmed its good specificity and sensitivity. The cell number of P. donghaiense correlated well with the optical density, and the regression equation is y=4× 10^- 6x＋ 0.694 9, in which x is the cell number, and y is the optical density, with r2.0.953 5. These results show that the NPA-SH method has good feasibility in the detection of P. donghaiense. Results of NPA-SH and microscopy are excellent for each sample. The NPA-SH method was a simple way in quantitative detection of P. donghaiense, and the whole process could be finished in about six hours, which provided a new approach in high-throughput sampling and long-term monitoring of P. donghaiense.

关键词： Prorocentrum donghaiense ribosomal RNA S1 enzyme sandwich hybridization integrated with nuclease protection assay (NPA-SH)

A maximum entropy distribution for wave heights of non-linear sea waves

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Progress in Natural Science:Materials International 2007年第11期17卷 1318-1326页

作者： Zhou Liangming1, Xu Delun1, Wang Aifang1, chen Zhengshou2.and Yan Xiuhua1(1. Physical oceanography Laboratory, ocean {2. of China, qingdao 2.6100,China 2.Engineering college, ocean {2. of China, qingdao 2.6071, China) Physical oceanography Laboratory Ocean University of China Qingdao 266100 China Engineering college Ocean University of China Qingdao 266071 China

Based on the maximum entropy principle,a probability density function (PDF) for the zero-crossing wave height (H) of random waves is derived as the simple form fn(H)=αHγe-βHn (n is a selectable positive integer) through solving a variational problem subject to some quite general constraints. This PDF maximizes the information entropy of H, and its parameters α, γ and β are expressed explicitly in terms of the distribution moments m, m=1,2.…,n, so it is well competent for describing the distribution of H of nonlinear sea waves with large uncertainty, and its parameters can be simply determined from available data. Comparisons between the PDF with n=3 and n=4 and the observed distributions of H from wave records measured in the East China Sea and in a wind-wave tunnel show fairly satisfying agreements.

关键词： sea waves the maximum entropy principle probability density function

Investigation of 'chazi'(damaged-enamels)on chenghua doucai Porcelains

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Science China(Technological Sciences) 2009年第6期52卷 1722-1729页

作者： DU Feng1 & SU BaoRu2.1.Institute of Chemistry & Molecular Engineering,qingdao {2. of Science & Technology,qingdao,China 2.college of Information Science & Technology,qingdao {2. of Science & Technology,qingdao,China Institute of Chemistry & Molecular Engineering Qingdao University of Science & Technology Qingdao China college of Information Science & Technology Qingdao University of Science & Technology Qingdao China

At the end of the 1950s,Sun Yingzhou considered that the 'chazi'(damaged colour)on chenghua doucai imperial porcelain as a defect was originally produced in firing process during chenghua period(1465―1487).Researchers in China have accepted his *** any fragment with the 'chazi' has never been found in several tons of chenghua imperial sherds excavated from Ming imperial kiln site in *** China,few scholars have questioned the formation of 'chazi',and further investigated *** researchers at the Research Laboratory in the British Museum,employing unde-structive EDXRF,have analyzed the chemical composition of the 'chazi'(damaged colour)on a chen-ghua doucai stem cup in the museum,and they have confirmed that the 'chazi'(damaged colour)was caused by the fire of the Jianfu palace in the Forbidden City in *** on their research,the authors studied the colour chemical principle of the 'chazi's' formation *** to the ceramic technology principle,scientific reasoning for the 'chazi' was carried *** the help of the history of Qing court,the brainteaser of the 'chazi' was totally uncovered,the misunderstanding for the 'chazi' has also been *** research also suggests that employing modern science and technology is a direction to study Chinese ancient ceramic further.

关键词： chenghua doucai porcelains court eunuchs the set on fire at the Jianfu palace ’chazi’(damaged-enamels) the British Museum colour chemical principle archaeology using science and technology

Influence of Lactobacillus E1 on the Storage Stability in Emulsion Immobilization

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Journal of Wuhan university of Technology(Materials Science) 2009年第1期24卷 75-80页

作者：孙欣陈西广 college of Life Science Ocean University of China

A coacervation method with double emulsion strategy （w/o/w） was used to prepare immobilized Lactobacillus E1. Diatomite was chosen as the carrier for bacteria. Sodium alginate, dextrin and gelatin were used as protective solutes for the preservation of Lactobacillus E1 and their effects on the storage viability during storage were discussed. The influence of storage temperature on the storage viability was also examined. The results show that high bacteria viable count over 109 cfu/g for an extended shelf life of 37 d can be achieved with 2. sodium alginate, 5% dextrin and 4% gelatin as protective solutes, at 10 ℃ of the storage temperature. This immobilized Lactobacillus E1 has potential use as functional food ingredient for both human dairy food and animal feedstuff.

关键词： lactobacillus microencapsulation storage viability protective solute shelf life

Direct adaptive neural control for stabilization of nonlinear time-delay systems

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Science China(Information Sciences) 2010年第4期53卷 800-812页

作者： WANG Min1,2., ZHANG SiYing2. chen Bing2.& LUO Fei2.1.1} of Automation, South China {2. of Technology, Guangzhou 510641, China 2.nstitute of Complexity Science, {2. {2., {2. 2.6071, China college of Automation South China University of Technology Guangzhou China Institute of Complexity Science Qingdao University Qingdao China

This paper is concerned with the problem of adaptive neural control for uncertain nonlinear strict-feedback time-delay systems with unknown virtual control coefficients. Radial basis function (RBF) neural networks are employed to directly approximate unknown virtual control signals, and then the adaptive neural control law is constructed by Lyapunov-Krasovskii functionals and backstepping. In order to avoid encountering a large number of adaptive parameters when using RBF neural networks as function approximators, an unknown constant, instead of unknown neural weights themselves, is employed as the estimated parameter. This technique makes only one adaptive parameter tuned online, thus significantly alleviating the burdensome computation. Meanwhile, some continuous functions are introduced to overcome the design difficulty originating from the use of one adaptive parameter. The proposed adaptive control guarantees the boundedness of all the signals in the closed-loop system. Simulation studies are presented to illustrate the effectiveness of the scheme.

关键词： nonlinear time-delay systems neural control adaptive control backstepping

Molecular mechanisms of polypeptide from Chlamys farreri protecting HaCaT cells from apoptosis induced by UVA plus UVB

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Acta Pharmacologica Sinica 2007年第7期28卷 1007-1014页

作者： Ming-qing GAO,Shen-bo GUO,Xue-hong chen,Wei DU,Chun-bo WANG~2.edical college,{2. {2.,{2. 2.602.,China Medical college Qingdao University Qingdao China

Aim:To investigate the mechanism of polypeptide from Chlamysfarreri (PCF)protecting HaCaT cells from apoptosis induced by UVA plus UVB in ***:An apoptotic model of UV irradiation-induced HaCaT cells *** 3-(4,5-dimethyl-2.thiazolyl)-2.5-diphenyl-2.-tetrazolium bromideassay,agarose gel electrophoresis,biochemical methods,and Western blottingwere employed in the ***:PCF inhibited the UV irradiation-inducedapoptosis of HaCaT *** strongly reduced the intracellular reactive oxygenspecies level,enhanced activities of superoxide dismutase and glutathione per-oxidase and increased the total anti-oxidative capacity in HaCaT cells followingUV ***,we found that PCF could inhibit the phosphorylationof c-Jun amino-terminal kinase and the activity of caspase-3 in a concentration-dependent ***:PCF protected HaCaT cells from apoptosis in-duced by UVA plus UVB,mainly through decreasing the intracellular ROS leveland increasing the activities of anti-oxidative enzymes to block the ROS-JNK-caspase-3-apoptosis signaling pathway.

关键词： polypeptide from Chlamys farreri antioxidant HaCaT apoptosis UV irradiation

Distribution and its mechanism of suspended particulate matters in the southern Huanghai Sea and the East China Sea in summer

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Acta oceanologica Sinica 2011年第5期30卷 94-100页

作者： QIAO Lulu LIU Yong chen Jiaojie MA Yanyan LI Guangxue SONG Jun Key Laboratory of Seafloor Science and Exploration Technology Ocean University of China Qingdao 266100 China college of Marine Geosciences Ocean University of China Qingdao 266100 China National Marine Data and Information Service State Oceanic Administration Tianjin 300171 China

Water temperature,turbidity,chlorophyll-a and suspended sediment concentration （SSC） were investigated at 61 stations of eight sections in the southern Huanghai Sea （HS） and the East China Sea （ECS） during the summer （2. June to 15 July） of *** horizontal distribution of suspended parti culate matter （SPM） displayed a high concentration inshore and a low value *** maximum value can reach 10.4 mg/dm 3,which can be found at the Changjiang River *** the same site,the SSC was generally higher at the bottom than on the *** the vertical direction,distribution characteristics of turbidity can be divided into two types：in the southern HS high values at the bottom while low values on the surface,and in the ECS high values inshore with low values *** thermocline in the HS and the Taiwan Warm Current in the ECS could be important factors preventing the SPM from diffusing upward and *** the typhoon Ewiniar was not able to work on the major sediment transport under the thermocline during the observation.

关键词： turbidity suspended particulate matter summer thermocline warm current

Molecular cloning,characterization and expression analysis of Sox9a and Foxl2.genes in half-smooth tongue sole(Cynoglossussemilaevis)

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Acta oceanologica Sinica 2011年第1期30卷 68-77页

作者： DONG Xiaoli chen Songlin JI Xiangshan SHAO Changwei college of fisher.es Ocean University of China Qingdao 266003 China Key Laboratory for Sustainable Utilization of Marine fisher.es Resources of Ministry ofAgriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesQingdao 266071 China college of Animal Science and Technology Shandong Agricultural University Taian 271018 China

Two transcription factors, Sox9a and Foxl2.were cloned from half-smooth tongue sole （Cynoglossus semilaevis）. Sox9a is a new duplication of *** Sox9 gene. The complete cDNA of Sox9a gene was 1 842.bp long coding for 487 amino acids and Foxl2.gene was 1 817 bp coding for 308 amino acids. Sox9a was expressed higher in male brain, pituitary and gonad and Foxl2.were higher in female brain, pituitary and gonad. The expression of Sox9a gene in gonads of neo-males was higher than that of normal females. Sox9a and Foxl2.were expressed higher in gastrula stage than in other stages. In the period of sex differentiation, the expression of Sox9a was first going up and then going down and Foxl2.was higher expressed at 37 dph. The highest expressions of Sox9a and Foxl2.genes occurred in nine-month and 12.month old gonad tissues, respectively. Sox9a gene was considered to have inevitable links with sex reversal, sex differentiation and cell differentiation of embryos and formation of spermatogenic ceils. Foxl2.was considered to play a role in sex differentiation, cell differentiation of embryos but not to be necessary for sex determination and sex reversal.

关键词： half-smooth tongue sole Cynoglossus semilaevis Sox9 Foxl2 real-time PCR sexreversal neo-male

Toxic effect of dinoflagellate Alexandrium species on Cladocera Moina mongolica Daday

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Acta oceanologica Sinica 2010年第2期29卷 120-126页

作者： YAN Tian chen Taoying ZHOU Mingjiang Institute of oceanology Chinese Academy of Sciences Qingdao 266071 China college of fisher.es and Life Science Shanghai Ocean University Shanghai 201306 China

The main objective of this study was to investigate toxic effects of the dinoflagellate Alexandrium species, on cladocera Moina mongolica in the laboratory. Ten strains of Alexandrium species, including Alexandrium tamarense （AT-6, ATHK, ATCI02. ATCI03 and AT5-1）, A. catenella （ACDH）, A. affine （AC-1 and AS-1）, A. lusitanicum and A A. tamarense （ATHK, ATCI02.and AT5-1） and A minutum were tested. The results showed that affine （AC-1 and AS-1）, but not A. tamarense （AT-6 and ATCI02., A. catenella （ACDH）, A. lusitanicum and A. minutum had significantly negative effects on the survival of M. mongoliea; when exposed to these ten strains of Alexandrium species in densities of 3 000 cells/cm^3 for 7 d, respecyively. M. mongolica could feed on A. tamarense （AT-6, ATHK, ATCI02. ATCI03 and AT5-1）, A. eatenella, A. lusitanieum and A. minutum, but exhibited little or no grazing on A. affine （AC-1 and AS-1）, based on the changes in gut pigment af- ter exposure to alga for 1 and 12.h. A. affine AC-1, which had the strongest toxicity on the survival of M. mongolica, was chosen to further study the negative effects on M. mongolica. The results showed that the effect of A. affine AC-1 on the survival of M. mongolica was density-dependent, and its lethal effects on one-day, two-day and three-day old M. mongolica were exacerbated with increasing age; The whole algal culture, re-suspended algal cells, cell fragments and cell contents all had adverse impacts on the survival of M. mongolica. Moreover, both juvenile numbers and life-span time of M. mongolica were decreased significantly when they were cultured in A. affine AC-1 of 10 and 50 cells/cm^3, combined with 3×10^6 cells/cm^3 of Chlorella spp., respectively. As a non-PSP producer, A. affine may produce other toxins, responsible for the strong negative effects on M. mongolica.

关键词： red tides Alexandrium species Moina mongolica Daday toxic effect