检索结果-南通市图书馆

Chinese Journal of Natural Medicines 2018年第12期16卷 951-960页

作者： DING Pei-Lan HE Chang-Ming chenG Zhi-Hong chen dao-feng Department of Pharmacognosy School of Pharmacy Fudan University

Sophorae Flavescentis Radix(Sophora flavescens Ait., SFR) and Sophorae Tonkinensis Radix et Rhizoma(S. tonkinensis Gapnep., STR) are two commonly used traditional Chinese medicines from Sophora(Leguminosae) plants, which are believed to possess similar bioactive components with entirely different clinical applications. In order to find out the characteristic chemical constituents potentially leading to the unique medicinal properties claimed for each of the two closely related TCMs, an HPLC fingerprint method was developed for analyses of the alkaloid and flavonoid constituents of SFR and STR, respectively, which were further evaluated and compared through similarity calculation and hierarchical clustering analysis(HCA). The results from the present study showed that the alkaloid fingerprints of the two herbs were similar, with many components co-existing in both drugs and various batches of samples from different species being mixed together in the HCA dendrogram. However, their flavonoid constituents were totally different with specific fingerprints being yielded for each herb, and further HCA analysis showed that the tested samples could almost be clearly divided into two groups based on their origins of species. The results from the present study indicated that the flavonoid constituents could serve as the differentially diagnostic constituents of SFR and STR and might potentially attributed to their distinct therapeutic effects.

关键词： Sophoraflavescens Sophora tonkinensis Alkaloids Flavonoids HPLC fingerprint Hierarchical clustering analysis

Preparative separation and quantitative determination of two kaurenoic acid isomers in root barks ofAcanthopanax gracilistylus

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2017年第8期15卷 625-630页

作者： XIE Xin-Xin JIANG Ze-Jing chenG Zhi-Hong chen dao-feng Department of Pharmacognosy School of PharmacyFudan UniversityShanghai 201203China

The kaurenoic acid-type diterpenoids in Acanthopanacis Cortex have been reported to be the major active components. However, the diterpenoids are present as position isomers that exacerbate the challenges in obtaining standards compounds. Little work has been done on the quantitative analysis of the diterpenoids in the herb. In the present study, two diterpenoid isomers ent-16βH,17-isovalerate-kauran-19-oic acid(1) and ent-16βH,17-methyl butanoate-kauran-19-oic acid(2) with high purity were separated by analytical HPLC, followed by recrystallization in acetone. Furthermore, an HPLC-ELSD method was developed and validated for simultaneous determination of 1 and 2 in 9 batches of Acanthopanacis Cortex samples. The HPLC separation and quantification was achieved in 40 min using an Agela Promosil C18 column eluted with a gradient of water and acetonitrile. The calibration curves showed good linearity(r^2 ≥ 0.999 9) within the test ranges. The LOD ranged from 0.407 2 to 0.518 0 mg and LOQ ranged from 1.018 0 to 1.295 0 mg. The precisions(%RSD) were within 1.47% for the two isomers. The recovery of the assay was in the range of 98.78%-99.11% with RSD values less than 2.76%. It is the first time to establish a quantitative HPLC method for the analysis of the bioactive kaurenoic acid isomers in the herb.

关键词： Acanthopanax gracilistylus Kaurenoic Acid Quantification HPLC-ELSD

Heteroclitins R-S: new dibenzocylooctadiene lignans from Kadsura heteroclita

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2014年第9期12卷 689-692页

作者： chen Min LUO You-Ping ZOU Yan-Lin LANG Ling-Hu chen dao-feng Key Laboratory on Luminescence and Real-Time Analysis (Ministry of Education) College of Pharmaceutical Sciences Southwest University College of Chemistry and Chemical Engineering HaiNan Normal University Department of Pharmacognosy School of Pharmacy Fudan University

AIM: To study the dibenzocylooctadiene lignans from the stems of Kadsura heteroclita. METHOD: Chromatographic separations of silica gel and semi-preparative HPLC were used. All of the structures were elucidated on the basis of spectroscopic analysis,including 2D-NMR and HR-MS techniques. RESULTS: Four dibenzocylooctadiene lignans were isolated from K. heteroclita. Their structures were identified as heteroclitin R(1), heteroclitin S(2), gonisin O(3), and schisanlignone A(4). CONCLUSION: Heteroclitin R(1) and heteroclitin S(2) are new natural lignans.

关键词： lignans spectroscopic elucidated silica chromato methoxy amorphous dried Cotton purified

Protective effects of Rabdosia japonica var. glaucocalyx extract on lipopolysaccharide-induced acute lung injury in mice

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2015年第10期13卷 767-775页

作者： XU Nai-Yu CHU Chun-Jun XIA Long ZHANG Jian chen dao-feng College of Pharmaceutical Science Soochow University Department of Pharmacognosy School of Pharmacy Fudan University

The present study was designed to evaluate the protective effects of ethanol extracts of Rabdosia japonica var. glaucocalyx(Maxim.) Hara(RJ) on lipopolysaccharide(LPS)-induced acute lung injury(ALI) in mice and the possible underlying mechanisms of action. The mice were orally administrated with RJ extract(16, 32 or 64 mg?kg–1) daily for consecutive7 days before LPS challenge. The ung specimens and the bronchoalveolar lavage fluid(BALF) were collected for histopathological examinations and biochemical analyses. Pretreatment with RJ significantly enhanced superoxide dismutase(SOD) activity and reduced the wet-to-dry weight(W/D) ratio, the levels of nitric oxide(NO) and protein leakage, and myeloperoxidase(MPO) activity in mice with ALI, in a dose-dependent manner. RJ reduced complement deposition and significantly attenuated LPS-induced ALI by reducing productions of inflammatory mediators, such as tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), and interleukin-1β(IL-1β). The results demonstrated that RJ may attenuate LPS-induced ALI via reducing the production of pro-inflammatory mediators, and reducing complement deposition and radicals.

关键词： Rabdosia japonica var.glaucocalyx(Maxim.) Hara Lipopolysaccharide Acute lung injury Complement 3

Polysaccharides extracted from the roots of Bupleurum chinense DC modulates macrophage functions

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2017年第12期15卷 889-898页

作者： ZHANG Zhi-De LI Hong WAN Fen SU Xiao-Yu LU Yan chen dao-feng ZHANG Yun-Yi Department of Pharmacology School of Pharmacy Fudan University Shanghai 201203 China Department of Pharmacognosy School of Pharmacy Fudan University Shanghai201203 China

The present study aimed to investigate the effects of polysaccharides extracted from Bupleurum chinense DC(BCPs) on macrophage functions. In the in vivo experiment, 1 m L of 5% sodium thioglycollate was injected into the abdomen of the mice on Day 0 and macrophages were harvested on Day 4. The macrophages were cultured in plates and treated with different concentrations of BCPs and stimulus. Effects of BCPs on macrophage functions were assessed by chemotaxis assay, phagocytosis assay and Enzyme-Linked Immunosorbent Assay(ELISA). Our results showed the enhanced chemotaxis, phagocytosis and secretion of nitric oxide(NO) and inflammatory cytokines by macrophages when treated with BCPs. However, when chemotaxis and phagocytosis were up-regulated by complement components or opsonized particles, BCPs inhibited these effects. Also, the NO production induced by lipopolysaccharides(LPS) was suppressed by BCPs mildly. Moreover, BCPs had an inhibitory effect on the [Ca^(2+)]i elevation of macrophages. These results suggested that BCPs exerted modulatory effects on macrophage functions, which may contribute to developing novel approaches to treating inflammatory diseases.

关键词： Bupleurum chinense DC Polysaccharides Macrophage functions [Ca^2+]i

Houttuynia cordata polysaccharide alleviated intestinal injury and modulated intestinal microbiota in H1N1 virus infected mice

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2019年第3期17卷 187-197页

作者： chen Mei-Yu LI Hong LU Xiao-Xiao LING Li-Jun WENG Hong-Bo SUN Wei chen dao-feng ZHANG Yun-Yi Department of Pharmacology School of Pharmacy Fudan University Department of Pharmacognosy School of Pharmacy Fudan University

Houttuynia cordata polysaccharide(HCP) is extracted from Houttuynia cordata, a key traditional Chinese medicine. The study was to investigate the effects of HCP on intestinal barrier and microbiota in H1N1 virus infected mice. Mice were infected with H1N1 virus and orally administrated HCP at a dosage of 40 mg×kg^(–1)×d^(–1). H1N1 infection caused pulmonary and intestinal injury and gut microbiota imbalance. HCP significantly suppressed the expression of hypoxia inducible factor-1α and decreased mucosubstances in goblet cells, but restored the level of zonula occludens-1 in intestine. HCP also reversed the composition change of intestinal microbiota caused by H1N1 infection, with significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera. Furthermore, HCP rebalanced the gut microbiota and restored the intestinal homeostasis to some degree. The inhibition of inflammation was associated with the reduced level of Toll-like receptors and interleukin-1β in intestine, as well as the increased production of interleukin-10. Oral administration of HCP alleviated lung injury and intestinal dysfunction caused by H1N1 infection. HCP may gain systemic treatment by local acting on intestine and microbiota. This study proved the high-value application of HCP.

关键词： H1N1 influenza virus Houttuynia cordata Inflammation Intestinal Barrier Microbiota Polysaccharide

Bupleurum polysaccharides ameliorated renal injury in diabetic mice associated with suppression of HMGB1-TLR4 signaling

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2019年第9期17卷 641-649页

作者： LIU Zhen-Zhen WENG Hong-Bo ZHANG Li-Jie PAN Ling-Yu SUN Wei chen Hai-Xia chen Mei-Yu ZENG Tao ZHANG Yun-Yi chen dao-feng LI Hong Department of Pharmacology School of PharmacyFudan UniversityShanghai 201203China Department of Pharmacognosy School of PharmacyFudan UniversityShanghai 201203China Clinical trial institution Obstetrics and Gynecology Hospital of Fudan UniversityShanghai 201203China

Bupleurum polysaccharides(BPs)is isolated from Bupleurum smithii ***,a key traditional Chinese *** study was to investigate the effects of BPs on diabetic kidney *** two intraperitoneal injections of streptozotozin(STZ)100 mg·kg^–1,renal injury in diabetic mice was induced and BPs was orally administrated at dosages of 30 and 60 mg·kg^–1·d^–*** STZ injected mice developed renal function damage,renal inflammation and fibrosis known as diabetic kidney disease(DKD).BPs significantly reduced serum creatinine level and urinary albumin excretion rate,with the attenuated swelling of *** treatment obviously alleviated the pathological damage of renal *** progression of renal injury in BPs treated mice was inhibited with less expression of type IV collagen(Col IV),fibronectin(FN)andα-smooth muscle actin(α-SMA).The inhibition of inflammation in kidney was associated with the reduced level of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6).BPs administration suppressed the over-expression of toll like receptor 4(TLR4)and high-mobility group box 1(HMGB1)with lowered activity of nuclear factor kappa B(NF-κB)in renal tissue of diabetic *** administration of BPs effectively prevented the development of renal injury in diabetic *** study suggested that the protection provided by BPs might affect through the interruption of HMGB1-TLR4 pathway,leading to the inhibition of renal inflammation and fibrotic process.

关键词： Renal injury Bupleurum:High-mobility group box Toll-like receptor 4 Inflammation Renal fibrosis Polysaccharides

Polymyxin B as an inhibitor of lipopolysaccharides contamination of herb crude polysaccharides in mononuclear cells

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2017年第7期15卷 487-494页

作者： LU Xiao-Xiao JIANG Yi-Fan LI Hong OU Ying-Ye ZHANG Zhi-De DI Hong-Ye chen dao-feng ZHANG Yun-Yi Department of Pharmacology School of Pharmacy Fudan University Shanghai 201203 China Department of Pharmacognosy School of Pharmacy Fudan University Shanghai 201203 China

Lipopolysaccharides(LPS) contamination in herbal crude polysaccharides is inevitable. The present study was performed to explore the effect of polymyxin B on abolishing the influence of LPS contamination in mononuclear cells. LPS was pretreated with polymyxin B sulfate(PB) at different concentrations for 1, 5 or 24 h, and then used to stimulate RAW264.7 and mouse peritoneal macrophages(MPMs). The nitric oxide(NO) and tumor necrosis factor-α(TNF-α) in cell culture supernatant, as the indications of cell response, were assayed. Bupleurum chinensis polysaccharides(BCPs) with trace amount contamination of LPS was treated with PB. 30 μg·mL^(–1) of PB, treating LPS(10 and 1000 ng·mL^(–1) in stimulating RAW264.7 and MPMs respectively) at 37 ℃ for 24 h, successfully abolished the stimulating effect of LPS on the cells. When the cells were stimulated with LPS, BCPs further promoted NO production. However, pretreated with PB, BCPs showed a suppression of NO production in MPMs and no change in RAW264.7. In the in vitro experiments, LPS contamination in polysaccharide might bring a great interference in assessing the activity of drug. Pretreatment with PB(30 μg·mL^(–1)) at 37 °C for 24 h was sufficient to abolish the effects of LPS contamination(10 and 1 000 ng·mL^(–1)).

关键词： Lipopolysaccharide Polymyxin B Bupleurum chinensis polysaccharides Mouse peritoneal macrophages RAW264.7 cells

比色法测定通光藤的C_(21)甾体糖苷成分含量

在线全文

学校读者我要写书评

暂无评论

中国中药杂志 2005年第22期30卷 1745-1748页

作者：沈帆陈道峰复旦大学药学院上海200032

目的：建立通光藤中C21甾体糖苷成分的含量测定方法，为通光藤药材的质量控制提供依据。方法：样品用甲醇提取，经ADS-7大孔吸附树脂纯化，以通光藤苷-H为对照品，浓硫酸-甲醇（4：1）显色，在325nm处测定通光藤中C2l甾体糖苷含量。结... 详细信息

目的：建立通光藤中C21甾体糖苷成分的含量测定方法，为通光藤药材的质量控制提供依据。方法：样品用甲醇提取，经ADS-7大孔吸附树脂纯化，以通光藤苷-H为对照品，浓硫酸-甲醇（4：1）显色，在325nm处测定通光藤中C2l甾体糖苷含量。结果：在10，6～148，4μg吸光度与含量成良好线性关系，r=0．9999（n=6），3个含量的加样回收率分别为95．4％，97．7％，96．9％，RSD〈2．4％（n=5）。结论：本法操作简便、快速、灵敏、重复性好，可以作为通光藤药材质量评价的定量分析方法。

关键词：通光藤 C21 甾体糖苷通光藤苷-H 比色法

在线全文

学校读者我要写书评

暂无评论

RP-HPLC法评价北豆根药材质量

复旦学报（医学版） 2007年第5期34卷 773-775页

作者：卢燕冯举陈道峰复旦大学药学院生药学教研室上海200032

目的探讨北豆根药材的质量评价标准。方法采用反相高效液相色谱法（RP-HPLC）测定10批北豆根商品药材中蝙蝠葛碱和蝙蝠葛苏林碱的含量。结果北豆根药材中蝙蝠葛碱和蝙蝠葛苏林碱分离完全且呈良好的线性关系，线性范围分别为0．992-4．96... 详细信息

目的探讨北豆根药材的质量评价标准。方法采用反相高效液相色谱法（RP-HPLC）测定10批北豆根商品药材中蝙蝠葛碱和蝙蝠葛苏林碱的含量。结果北豆根药材中蝙蝠葛碱和蝙蝠葛苏林碱分离完全且呈良好的线性关系，线性范围分别为0．992-4．96μg和1．04-5．20μg；平均加样回收率分别为100．2％（RSD=1．46％）和100．5％（RSD=1．36％）。不同购买地的药材两种生物碱含量差异较大，蝙蝠葛碱为0．092％～0．844％，蝙蝠葛苏林碱为0．322％～1．016％。两种生物碱总含量为0．688％～1．226％，相对较稳定。结论蝙蝠葛碱和蝙蝠葛苏林碱的总含量可作为北豆根药材质量控制指标。

关键词：北豆根蝙蝠葛碱蝙蝠葛苏林碱 RP-HPLC 质量控制