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Generation of male germ cells in vitro from the stem cells

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Asian Journal of Andrology 2023年第1期25卷 13-20页

作者： Ying-Hong Cui wei chen Si Wu Cai-Lin Wan Zuping He The Key Laboratory of Model Animals and Stem Cell Biology in Hunan Province Hunan Normal University School of MedicineThe Manufacture-Based Learning and Research Demonstration Center for Human Reproductive Health New Technology of Hunan Normal UniversityChangsha 410013China

Infertility has become a serious disease since it affects 10%–15%of couples worldwide,and male infertility contributes to about 50%of the ***,a significant decrease occurs in the newborn population by 7.82 million in 2020 compared to 2016 in *** such,it is essential to explore the effective methods of obtaining functional male gametes for restoring male *** cells,including embryonic stem cells(ESCs),induced pluripotent stem cells(iPSCs),spermatogonial stem cells(SSCs),and mesenchymal stem cells(MSCs),possess the abilities of both self-renewal and differentiation into germ ***,much progress has recently been achieved in the generation of male germ cells in vitro from various kinds of stem cells under the specified conditions,e.g.,the coculturing with Sertoli cells,three-dimensional culture system,the addition of growth factors and cytokines,and/or the overexpression of germ cell-related *** this review,we address the current advance in the derivation of male germ cells in vitro from stem cells based on the studies of the peers and us,and we highlight the perspectives and potential application of stem cell-derived male gametes in reproductive medicine.

关键词： application in vitro generation male germ cells stem cells

Characterization of the Complete Mitochondrial Genome of the Hybrid Epinephelus moara♀ × Epinephelus lanceolatus♂,and Phylogenetic Analysis in Subfamily Epinephelinae

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Journal of Ocean University of China 2017年第3期16卷 555-563页

作者： GAO Fengtao wei Min ZHU Ying GUO Hua chen Songlin YANG Guanpin College of Marine Life Science Ocean University of China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences (CAFS)Key Laboratory for Sustainable Development of Marine FisheriesMinistry of Agriculture Wuxi Fisheries College Nanjing Agricultural University

This study presents the complete mitochondrial genome of the hybrid Epinephelus moara♀× Epinephelus lanceolatus♂. The genome is 16886 bp in length, and contains 13 protein-coding genes, 2 r RNA genes, 22 t RNA genes, a light-strand replication origin and a control region. Additionally, phylogenetic analysis based on the nucleotide sequences of 13 conserved protein-coding genes using the maximum likelihood method indicated that the mitochondrial genome is maternally inherited. This study presents genomic data for studying phylogenetic relationships and breeding of hybrid Epinephelinae.

关键词： Epinephelus moara♀× Epinephelus lanceolatus♂ mitochondrial genome phylogenetic analysis

Effects of protein and lignin on cellulose and xylan anaylses of lignocellulosic biomass

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Journal of Integrative Agriculture 2017年第6期16卷 1268-1275页

作者： James MacLellan Rui chen Zhengbo Yue Robert Kraemer Yan Liu wei Liao Department of Biosystems and Agricultural Engineering Michigan State University School of Resources and Environmental Engineering Hefei University of Technology

Interactions of lignocellulosic components during fiber analysis were investigated using the highly adopted compositional analysis procedure from the National Renewable Energy Laboratory（NREL）,*** feedstock samples were used to study the effects of lignin/protein,cellulose/protein,and xylan/protein interaction on carbohydrate *** structural influence in the synthetic samples,lignin and protein components were the most significant（P〈0.05）factors on cellulose *** xylan was consistent and unaffected by content variation throughout the synthetic *** of the observed relationships from synthetic feedstocks was fulfilled using real lignocellulosic feedstocks：corn stover,poplar,and alfalfa,in which similar results have been obtained,excluding cellulose analysis of poplar under higher protein content and xylan analysis of alfalfa under higher protein *** results elucidated that according to their protein and lignin contents of different lignocellulosic materials,accuracy of the NREL method on cellulose and xylan analyses could be improved by applying a stronger extraction step to replace water/ethanol extraction.

关键词： lignocellulosic biomass cellulose xylan lignin protein

Modeling transport of arsenic through modified granular natural siderite filters for arsenic removal

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Geoscience Frontiers 2019年第5期10卷 1755-1764页

作者： Fulan Li Huaming Guo Kai Zhao wei Xiu Jiaxing Shen Yi chen State Key Laboratory of Biogeology and Environmental Geology MOE Key Laboratory of Groundwater Circulation and Environment EvolutionChina University of Geosciences (Beijing)Beijing 100083China School of Water Resources and Environment China University of Geosciences (Beijing)Beijing 100083China China Institute for Geo-environmental Monitoring Beijing 100081China

Groundwater arsenic (As) contamination is a hot issue,which is severe health concern ***,many Fe-based adsorbents have been used for As removal from *** granular natural siderite (MGNS),a special hybrid Fe(II)/Fe(III) system,had higher adsorption capacity for As(III) than As(V),but the feasibility of its application in treating high-As groundwater is still *** combination with transport modeling,laboratory column studies and field pilot tests were performed to reveal both mechanisms and factors controlling As removal by MGNS-filled *** show that weakly acid pH and discontinuous treatment enhanced As(III) removal,with a throughput of 8700 bed volumes (BV) of 1.0 mg/L As(III) water at breakthrough of 10 mg/L As at pH *** HCO3^- inhibited As removal by the *** mineral species,SEM and XRD patterns of As-loading MGNS show that the important process contributing to high As(III) removal was the mineral transformation from siderite to goethite in the *** homogeneous surface diffusion modeling (HSDM) shows that competition between As(III) and HCO3^- with adsorption sites on MGNS was *** inhibition of HCO3^- on As(III) removal was connected to inhibition of siderite dissolution and mineral *** loadings were lower in field pilot tests than those in the laboratory experiments,showing that high concentrations of coexisting anions (especially HCO3^-- and SiO4^4-),high pH,low EBCT,and low groundwater temperature decreased As *** was suggested that acidification and aeration of high- As groundwater and discontinuous treatment would improve the MGNS filter performance of As removal from real high-As groundwater.

关键词： Arsenite (As) Adsorption Mineral transformation Column Groundwater

VisComposer: A Visual Programmable Composition Environment for Information Visualization

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Visual Informatics 2018年第1期2卷 71-81页

作者： Honghui Mei wei chen Yuxin Ma Huihua Guan Wanqi Hu State Key Lab of CAD&CG Zhejiang UniversityHangzhouChina

As the amount of data being collected has increased,the need for tools that can enable the visual exploration of data has also *** has led to the development of a variety of widely used programming frameworks for information ***,such frameworks demand comprehensive visualization and coding skills and require users to develop visualization from *** alternative is to create interactive visualization design environments that require lttle to no ***,these tools only supports a small portion of visual *** present a programmable integrated development environment(IDE),VisComposer,that supports the development of expressive visualization using a drag and-drop visual ***-Composer exposes the programmability by customizing desired components within a modular-ized visualization composition pipeline,effectively balancing the capability gap between expert coders and visualization *** implemented system empowers users to compose compre-bensive visualizations with real-time preview and optimization features,and supports prototyp-ing,sharing and reuse of the effects by means of an intuitive visual *** program-ming and textual programming integrated in our system allow users to compose more complex visual effects while retaining the simplicity of *** demonstrate the performance of VisCom-poser with a variety of examples and an informal user evaluation.

关键词： Information Visualization Visualization authoring Interactive development environment

Effect of mutant p27^(kip1) gene on human cholangiocarcinoma cell line, QBC_(939)

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World Journal of Gastroenterology 2008年第34期14卷 5344-5348页

作者： Jian Luo Yong-Jun chen wei-Yu Wang Sheng-Quan Zou Department of General Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei Province China

AIM：To investigate the effects of exogenously mutated p27^kip1 （p27） on proliferation and apoptosis of human cholangiocarcinoma cell line, QBC939 in ***： Adenviral vectors were used to transfect mutated p27 cDNA into human QBC939 cell line. Expression of p27 was detected by RT-PCR. Western blot. Cell growth, morphological change, cell cycle, apoptosis and cloning formation were determined by MTT assay and flow ***： The expression of p27 protein and mRNA was increased signifi cantly in QBC939 cell line transfected with Ad-p27mt. The transfer of Ad-p27mt could signifi cantly inhibit the growth of QBC939 cells, decrease the cloning formation rate and induce apoptosis. p27 over expression caused cell cycle arrest at G0/G1 phase 72 h after infection with ***： p27 may cause cell cycle arrest at G0/G1 phase and subsequently lead to apoptosis. Recombinant adenovirus expressing mutant p27 may be potentially useful in gene therapy for cholangiocarcinoma.

关键词： Adenovirus Cholangiocarcinoma Gene therapy Cell cycle Apoptosis

Control of redox potential by oxygen limitation in selective bioleaching of chalcocite and pyrite

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Rare Metals 2014年第5期33卷 622-627页

作者： Biao Wu Jian-Kang Wen Bo-wei chen Guo-cheng Yao Dian-Zuo Wang National Engineering Laboratory of Biohydrometallurgy General Research Institute for Nonferrous Metals

Based on the bioleaching mechanism and electrochemical studies of metal sulfides, the dissolution rates of chalcocite and pyrite are controlled by redox potentials. Experiment on the bioleaching of chalcocite and pyrite under constant redox potential by sparging with nitrogen gas was demonstrated. By leaching at low and constant redox potential（〈760 mV, vs SHE）, copper recoveries of 90 %–98 % are achieved, which are 10 times more than iron recoveries. The iron-oxidizing bacterial populations are observed to continue to reduce under oxygen limitation conditions, but the Acidithiobacillus that have only sulfur-oxidizing capabilities are an attractive alternative for redox-controlled bioleaching of ***, the described redox control technique might be one of the effective approaches to balance acid and iron in Zijinshan copper bio-heap leaching practice.

关键词： Pyrite Chalcocite Bioleaching Redox potential Oxygen limitation

Fluorescent AIE dots encapsulated organically modified silica (ORMOSIL) nanoparticles for two-photon cellular imaging

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Science China Chemistry 2013年第9期56卷 1247-1252页

作者： ZHU ZhenFeng ZHAO XinYuan QIN wei chen GuangDi QIAN Jun XU ZhengPing Centre for Optical and Electromagnetic Research Zhejiang Provincial Key Laboratory for Sensing TechnologiesZhejiang University Bioelectromagnetics Laboratory School of MedicineZhejiang University Department of Chemistry The Hong Kong University of Science and Technology

2,3-Bis(4-(phenyl(4-(1,2,2-triphenylvinyl)phenyl)amino)phenyl) fumaronitrile (TPE-TPA-FN or TTF), which possesses aggregation-induced emission (AIE) characteristic, is doped in organically modified silica (ORMOSIL) nanoparticles. By increasing the weight ratio of TTF to the precursor of silica nanoparticles (the quantities of the precursors were kept the same), the fluorescence intensity of nanoparticles increased correspondingly, due to the formation of larger AIE dots in the cores of ORMOSIL nanoparticles. The fluorescent and biocompatible nanoprobes were then utilized for in vitro imaging of HeLa cells. Two-photon fluorescence microscopy clearly illustrated that the nanoparticles have the capacity of nucleus permeability, as well as cytoplasm staining towards tumor cells. Our experimental results may offer a promising method for fast and bright fluorescence imaging, as well as bio-molecule/drug delivery to cell nucleus.

关键词： TTF aggregation-induced emission organically modified silica two-photon nucleus permeability

Lactate Promotes Cancer Stem-like Property of Oral Sequamous Cell Carcinoma

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Current Medical Science 2019年第3期39卷 403-409页

作者： Hui ZHAO Chuan-yu HU wei-min chen Ping HUANG Department of Stomatology Tongji Hospital Tongji Medical College Huazhong University of Science and TechnologyWuhan 430030 China

Accumulation of lactate in tumor has been linked to poor prognosis of oral squamous cell carcinoma (OSCC), but the underlying mechanism remained largely uncertain. Previous studies have suggested that presence of cancer stem cells (CSCs) closely correlated with cellular malignancy of OSCC. Here, using 3D organoid culture model, we investigated whether lactate promoted CSCs phenotype in primary OSCC cells. We generated organoids using fresh OSCC specimens and verified that organoids recapitulated histopathology and cellular heterogeneity of parental tumor;Organoids were then transfected with a Wnt reporter to visualize Wnt activity. The sphere forming assay demonstrated that high Wnt activity functionally designated CSCs population in OSCC cells. Further investigations indicated that lactate treatment promoted Wnt activity and increased the expression of CSCs (i.e. CD133^+ cells) in organoids. Moreover, silencing monocarboxylate transporter 1 (MCT1), the prominent path for lactate uptake in human tumor with siRNA significantly impaired organoid forming capacity of OSCC cells. Together, our study demonstrated that lactate can promote CSCs phenotype of OSCC,and MCT1 may be a therapeutic target against OSCC growth.

关键词： oral squamous cell carcinoma lactate cancer stem cells organoid monocarboxylate transporter1