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Chemical synthesis of a structure gene coding for small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase from tobacco

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Chinese Journal of chemistry 1994年第4期12卷 355-364页

作者： chen, hai-bao weng,{2., jian-shaoshanghai institute of organic chemistry, chinses academy of Sciences, 354 fenglin lu, shanghai 2.0032. china shanghai institute of {2. chemistry Chinses Academy of Sciences 354 Fenglin Lu Shanghai 200032 China

A structural gene with 385 bp, which codes for the mature small subunit (rbcS) of ribulose-1,5-bi9phosphate carboxylase/oxygenase from tobacco, has been redesigned according to the codon usage of *** and chemically synthesized. To facilitate the systematic investigation of structure-functional relationship of the rbcS by site-specific mutagenesis, twenty one unique restriction sites distributed throughout the synthetic gene were designed. Gene synthesis was started from chemically synthesizing sixteen oligonucleotides each with 2.-66 nucleotides, and these oligonu-cleotides were annealed to form eight duplexes from which 5'- and 3'- two half molecules were formed by stepwise T4 DNA ligase reaction, and then each half molecule was cloned into plasmid pWR13, after that, two half molecules were further confirmed by DNA sequencing, finally both half molecules excised from the cloning plasmid were recombinated to form plasmid pCOTrbcS containing the whole structural gene for tobacco rbcS.

关键词： Gene synthesis ribulose-1,5-bisphosphate carboxylase/oxygenase clone.

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Studies on the Diastereoselective Alkylation Reaction Employing Isomannide and Isosorbide as Chiral Auxiliaries

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Chinese Chemical Letters 1998年第4期9卷 325-328页

作者： jian Qiang WANG ming Hua XU min ZHONG Guo Qiang LIN(shanghai institute of organic chemistry, Chinese academy of Sciences354 fenglin lu, shanghai 2.0032. Chinese Acad Sci Shanghai Inst Organ Chem Shanghai 200032 Peoples R China

The studies on the asymmetric alkylation of ester employing D-Isomannide and D-isosorbide as chiral auxiliaries were described. The diastereomeric excess of this reaction was up to 90%.

关键词： diastereoselective alkylation isomannide isosorbide

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Synthesis and mesomorphic behaviour of 4'-methoxyphenyl 4"-[(4-n-alkoxy-2.3,5,6-tetrafluorophenyl) ethynyl] benzylethers

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Chinese Journal of chemistry 1995年第1期13卷 73-78页

作者： WEN, jian-Xun YIN, Hui-Yong TIAN, min-Quan {2.G, Qishanghai institute of organic chemistry, Chinese academy of Science, 354 fenglin lu, shanghai 2.0032. china shanghai institute of {2. chemistry Chinese Academy of Science S54 Fenglin Lu Shanghai 200032 China

A novel homologous series of 4'-methyoxyphenyl 4'-[(4-n-alkoxy-2.3,5,6-tetrafluoro-phenyl]benzyl ethers have been prepared via Pd-catalysed Heck reactions. Polarizing microscopic textural observation shows that these ... 详细信息

关键词： Liquid crystal nematic systhesis.

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