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肠道菌群在急性肾损伤中作用的研究进展

中南大学学报（医学版） 2024年第3期49卷 385-391页

作者：陈天笑常畅侯豹邱丽颖孙海建朱雪雪江南大学无锡医学院基础医学系江苏无锡214122

急性肾损伤(acute kidney injury,AKI)是一个全球性的公共卫生问题,其发病率和病死率高、医疗费用昂贵且治疗手段有限。AKI可进一步转变为慢性肾脏病(chronic kidney disease,CKD),最终进展为终末期肾病(endstage renal disease,ESRD)... 详细信息

急性肾损伤(acute kidney injury,AKI)是一个全球性的公共卫生问题,其发病率和病死率高、医疗费用昂贵且治疗手段有限。AKI可进一步转变为慢性肾脏病(chronic kidney disease,CKD),最终进展为终末期肾病(endstage renal disease,ESRD)。既往研究表明,创伤、药物的不良反应、手术等与AKI密切相关。随着进一步的深入探索,肠道菌群在AKI中的作用逐渐被揭示。AKI发生后,肠道菌群组成改变,肠道屏障破坏引发肠道免疫以及肠道细菌易位。同时,肠道菌群的代谢产物又可以加剧AKI的进展。因此,阐述肠道菌群参与AKI发生和发展的具体机制,有助于从肠道微生物角度为AKI的防治提供新思路。

关键词：急性肾损伤肠道菌群炎症毒素短链脂肪酸益生菌后生元中药

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Intermetallic phase formation and evolution during homogenization and solution in Al-Zn-Mg-Cu alloys

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Science China(Technological Sciences) 2013年第11期56卷 2827-2838页

作者： LI ChunMei chen ZhiQian ZENG SuMin chenG NanPu chen tianxiao School of Materials Science and Engineering Central South University School of Materials Science and Engineering Southwest University

The effects of major alloy element contents of Zn, Mg, Cu in Al-Zn-Mg-Cu alloys on the formation and evolution of intermetallic phases during casting, homogenization and solution treatment have been investigated through using X-ray diffraction, scanning electron microscopy and differential scanning calorimetry. Experimental results showed that a relatively higher Zn content with lower Mg and Cu contents was beneficial to the formation of MgZn2 phase instead of the A12CuMg phase, which resulted in the unicity of the intermetallics in the A1 matrix, and that the MgZn2 phase was easier for diffusion and dissolution during homogenization and solution than the Al2CuMg phase. Additionally, the results of the first-principles calculations gave support for explaining the experimental phenomena. A larger absolute value of formation enthalpy and a smaller value of binding energy of the MgZn2 phase, as compared with the Al2CuMg phase, give it priority to precipitate during casting and make it easier to re-dissolve during homogenization and solution treatment. What＇s more, higher elastic constants with severe anisotropy of Young＇s modulus make undissolved blocks of AI^CuMg phase act as crack initiation, which degrade the perfor- mance of the materials.

关键词： intermetallic phases homogenization solution first-principles Al alloys

A Label-free and Functional Fluorescent Oligonucleotide Probe Based on a G-Quadruplex Molecular Beacon for the Detection of Kanamycin

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Chemical Research in Chinese Universities 2018年第4期34卷 541-545页

作者： ZHU Yuqing LI Wei TAN Suzhen chen tianxiao School of Chemistry and Biological Engineering Changsha University of Science and Technology Changsha 410004 P. R. China

A label-free and turn-off fluorescent method for the quantitative detection of kanamycin based on a funtional molecular beacon was developed. The molecular beacon consists of two hairpin structures with a split G-rich oligonucleotide in the middle. The kanamycin＇s aptamer formed the loops portion for recognizing kanamycin, and the G-quadruplex bound by Thioflavin T（ThT） was employed as the reporter. In the absence of target, the molecular beacon folded into double stem-loops and the splited G-rich oligonucleotid came close to form a G-quadruplex. When ThT bound to the G-quadruplex, the fluorescence intensity of the solution increased. Upon the addition of kanamycin, the function between kanamycin and aptamer unfolded the hairpin and disassembled the G-quadraplex structure, resulting in a significant decrease in the fluorescence intensity. A good linear relationship ranging from 0.7 nmol/L to 10 nmol/L was achieved and the limit of detection was 0.37 nmol/L. Besides, it could efficiently recognize kanamycin in real samples.

关键词： Molecular beacon Fluorescence Kanamycin G-Quadruplex Thioflavin T

基于指数函数的川滇地区余震序列衰减规律研究

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地震工程学报 2024年第1期46卷 207-213页

作者：徐世达段俊康何本国刘佳奇鲍泽铭陈天晓东北大学深部金属矿山安全开采教育部重点实验室辽宁沈阳110819

较大的余震可能造成额外损失并有二次触发建筑物受灾的风险。为研究余震序列衰减规律,文章尝试采用指数衰减模型拟合分析5个不同地区余震序列,并借助修正赤池信息准则、贝叶斯信息准则与调整后R^(2),分析其与传统余震衰减模型的性能。... 详细信息

较大的余震可能造成额外损失并有二次触发建筑物受灾的风险。为研究余震序列衰减规律,文章尝试采用指数衰减模型拟合分析5个不同地区余震序列,并借助修正赤池信息准则、贝叶斯信息准则与调整后R^(2),分析其与传统余震衰减模型的性能。结果表明,指数模型描述余震序列衰减规律的能力与修正的大森余震模型、修正的拉伸指数模型接近。尤其对于四川长宁M_(S)6.0余震序列和云南彝良M_(S)5.7余震序列,指数模型表现优于其他两种模型。指数模型参数具有明确的物理意义:参数A与r之和能够准确代表强震后的实际初始余震数,5个余震序列初始余震数偏差均小于1.70%;参数k可作为反映余震序列衰减快慢的特征值,k值越大则余震序列衰减越慢,其值与主震震级呈反比例关系。

关键词：余震序列指数函数初始余震数衰减

王不留行黄酮苷通过miR-570-3p/BNIP3减轻线粒体损伤并缓解2型糖尿病内皮功能障碍

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中国病理生理杂志 2024年第5期40卷 872-881页

作者：赵晨阳朱雪雪陈欣雨陈天笑徐锦朋李泰悦曹幸予张源邱丽颖江南大学无锡医学院江苏无锡214122

目的:探究王不留行黄酮苷(VAC)对2型糖尿病(T2DM)内皮功能障碍的治疗作用及机制研究。方法:(1)通过腹腔注射链脲佐菌素和喂饲高脂饲料(21.8 kJ/kg,60%能量为脂肪)构建T2DM小鼠模型。将30只C57BL/6小鼠随机分为对照组、T2DM组和T2DM+VAC... 详细信息

目的:探究王不留行黄酮苷(VAC)对2型糖尿病(T2DM)内皮功能障碍的治疗作用及机制研究。方法:(1)通过腹腔注射链脲佐菌素和喂饲高脂饲料(21.8 kJ/kg,60%能量为脂肪)构建T2DM小鼠模型。将30只C57BL/6小鼠随机分为对照组、T2DM组和T2DM+VAC组,每组10只。T2DM+VAC组小鼠给予1 mg/kg VAC灌胃6周,对照组和T2DM组小鼠均给予等体积PBS。RT-qPCR和Western blot检测胸主动脉中BCL2相互作用蛋白3(BINIP3)、PTEN诱导激酶1(PINK1)和parkin的mRNA和蛋白表达。(2)在体外通过高糖(HG)刺激人脐静脉内皮细胞(HUVECs),用JC-1染色检测线粒体膜电位变化,吖啶橙(AO)染色检测自噬溶酶体变化,MitoSOX染色检测线粒体超氧化物的积累。结果:与对照组相比,T2DM小鼠胸主动脉BNIP3、PINK1和parkin的mRNA和蛋白表达水平显著升高(P<0.05);与T2DM组相比,T2DM+VAC组小鼠胸主动脉中BNIP3、PINK1和parkin的mRNA和蛋白表达水平显著降低(P<0.05)。JC-1、AO和MitoSOX染色结果显示,VAC可抑制HG诱导的HUVECs线粒体膜电位降低、自噬溶酶体增多和线粒体超氧化物水平升高。VAC也可减轻BNIP3过表达后HG诱导的HUVECs线粒体损伤。微小RNA-570-3p(miR-570-3p) mimic与VAC有相似的减轻线粒体损伤作用。RT-qPCR和Western blot结果显示,miR-570-3p mimic和VAC都使BNIP3、PINK1和parkin的mRNA和蛋白水平显著降低。使用miR-570-3p抑制剂后,VAC的上述作用均被抑制。结论:VAC通过miR-570-3p/BNIP3减轻HG诱导的线粒体损伤,从而缓解T2DM内皮功能障碍。

关键词：王不留行黄酮苷 2型糖尿病内皮功能障碍 BNIP3蛋白微小RNA-570-3p 线粒体损伤

王不留行黄酮苷改善糖尿病肾病小鼠肠道菌群紊乱和肾脏脂质沉积的研究

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华西药学杂志 2024年第1期39卷 36-42页

作者：于晓依常畅陈天笑马琳春陈欣雨蔡维维邱丽颖江南大学无锡医学院江苏无锡214122

目的研究王不留行黄酮苷(Vac)对糖尿病肾病小鼠肾脏脂质合成的影响及其机制。方法通过高脂饲料喂养和腹腔注射链脲佐菌素构建糖尿病肾病小鼠模型。小鼠随机分为正常组、模型组和治疗组。采用血糖仪检测小鼠空腹血糖;采用试剂盒检测小鼠... 详细信息

目的研究王不留行黄酮苷(Vac)对糖尿病肾病小鼠肾脏脂质合成的影响及其机制。方法通过高脂饲料喂养和腹腔注射链脲佐菌素构建糖尿病肾病小鼠模型。小鼠随机分为正常组、模型组和治疗组。采用血糖仪检测小鼠空腹血糖;采用试剂盒检测小鼠尿液中尿素氮、尿肌酐、24 h尿蛋白、尿蛋白肌酐等的含量,血清中血肌酐、甘油三酯、总胆固醇、低密度脂蛋白、高密度脂蛋白等的含量,肾脏组织中甘油三酯和总胆固醇的含量;采用HE和PAS染色法观察小鼠肾脏的病理学变化;采用总胆汁酸试剂盒测定小鼠血清中总胆汁酸的含量;通过实时荧光定量PCR、免疫印迹和免疫组化分别检测小鼠肾脏组织中固醇调节元件结合蛋白-1和脂肪酸合成酶的mRNA表达,法尼醇X受体(FXR)和G蛋白偶联胆汁酸受体5(TGR5)的mRNA和蛋白表达水平。结果与正常组比较,模型组小鼠的血糖升高、血脂增加,肾脏脂质沉积加剧,肾功能出现损伤;经Vac治疗后,糖尿病肾病小鼠可缓解脂质代谢紊乱,改善小鼠的肾脏功能。模型小鼠的肠道菌群失调,在门和属水平上的有益菌减少,有害菌增加;Vac干预可明显改善了这一情况,同时Spearman相关性分析也显示小鼠脂质代谢水平与肠道菌群存在显著相关性。与正常组比较,模型组小鼠血清中总胆汁酸的含量增加,胆汁酸受体FXR和TGR5受体的表达显著降低;Vac可降低总胆汁酸的含量,上调FXR和TGR5受体的表达。结论Vac可通过调节肠道菌群微生物及其代谢物胆汁酸的水平,缓解模型小鼠脂质沉积,可能是改善糖尿病肾病患者肾脏脂质沉积的有效候选药物。

关键词：王不留行黄酮苷糖尿病肾病空腹血糖肾功能脂质代谢肠道菌群胆汁酸胆汁酸受体

Preparation of core-shell magnetic Fe_3O_4@SiO_2-dithiocarbamate nanoparticle and its application for the Ni^(2+), Cu^(2+) removal

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Chinese Chemical Letters 2018年第6期29卷 887-891页

作者： Yimin Dai Lanli Niu Jiaqi Zou tianxiao chen Hui Liu Yi Zhou School of Chemistry and Biological Engineering Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation Changsha University of Science and Technology

A novel magnetic nanoparticles-based dithiocarbamate absorbent（Fe3O4@SiO2-DTC） with core-shell structure was synthesized under mild conditions and used in aqueous solution Ni2＋ and Cu2＋ ions treatment. The structure, morphology and magnetic properties of the adsorbent were characterized by Xray diffraction（XRD）, fourier transformed infrared spectroscopy（FTIR）, scanning electron microscopy（SEM）, transmission electron microscopy（TEM）, and vibrating sample magnetometer（VSM）.Fe3O4@SiO2-DTC exhibited a typical superparamagnetic with a saturation magnetization value of52.7 emu/g, which could be rapidly separated from aqueous solution under external magnetic field. We investigated the effects of solution p H, adsorption time, and the initial concentration of heavy metal ions on the adsorption of Ni2＋ and Cu2＋. The adsorption equilibrium times of Ni2＋and Cu2＋ on Fe3 O4@SiO2-DTC were reached at 15 min and 90 min, respectively. The adsorption kinetic data were fitted to the pseudosecond-order model, and the adsorption data were consistent with the Frenudlich isotherm model. When the initial concentration of heavy metal ions was 250 mg/L, the maximum adsorption capacity of Ni2＋ and Cu2＋ at room temperature was 235.23 mg/g and 230.49 mg/g, respectively. In addition, we discussed the plausible adsorption mechanism. The results indicated that the adsorption was mainly dominated by chelation.

关键词： Core-shell Magnetic nanoparticle Dithiocarbamate Adsorption Heavy metals removal

Molecular mechanisms of salinity tolerance in rice

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The Crop Journal 2021年第3期9卷 506-520页

作者： tianxiao chen Sergey Shabala Yanan Niu Zhong-Hua chen Lana Shabala Holger Meinke Gayatri Venkataraman Ashwani Pareek Jianlong Xu Meixue Zhou Tasmanian Institute of Agriculture University of TasmaniaPrivate Bag 1375ProspectTAS 7250Australia Guangdong Laboratory of Lingnan Modern Agriculture Genome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120GuangdongChina Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement Chinese Academy of Agricultural SciencesBeijing 100081China International Research Centre for Environmental Membrane Biology Foshan UniversityFoshan 528000GuangdongChina School of Science Hawkesbury Institute for the EnvironmentWestern Sydney UniversityPenrithNSW 2751Australia University of Tasmania Private Bag 51HobartTAS 7001Australia Plant Molecular Biology Laboratory M.S.Swaminathan Research FoundationⅢCross StreetTaramani Institutional AreaChennai 600113India School of Life Sciences Jawaharlal Nehru UniversityNew Delhi 110067India National Agri-Food Biotechnology Institute Mohali 140306India

Salinity is one of the major abiotic stresses which impose constraints to plant growth and ***(Oryza sativa L.)is one of the most important staple food crops and a model monocot *** production is expanding into regions that are affected by soil salinity,requiring cultivars more tolerant to saline *** the molecular mechanisms of such tolerance could lay a foundation for varietal improvement of salt tolerance in *** spite of extensive studies exploring the mechanism of salt tolerance,there has been limited progress in breeding for increased salinity *** this review,we summarize the information about the major molecular mechanisms underlying salinity tolerance in rice and further discuss the limitations in breeding for salinity *** show that numerous gene families and interaction networks are involved in the regulation of rice responses to salinity,prompting a need for a comprehensive functional *** also show that most studies are based on whole-plant level analyses with only a few reports focused on tissue-and/or cell-specific gene *** details of salt-responsive channel and transporter activities at tissue-and cell-specific level still need to be documented before these traits can be incorporated into elite rice ***,future studies should focus on diversity of available genetic resources and,particular,wild rice relatives,to reincorporate salinity tolerance traits lost during domestication.

关键词： Oryza sativa L. Reactive oxygen species(ROS) Stomatal regulation Membrane transporters Osmotic adjustment Gene network Crosstalk

Sdr4 dominates pre-harvest sprouting and facilitates adaptation to local climatic condition in Asian cultivated rice

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Journal of Integrative Plant Biology 2022年第6期64卷 1246-1263页

作者： Bo Zhao Hui Zhang tianxiao chen Ling Ding Liying Zhang Xiali Ding Jun Zhang Qian Qian Yong Xiang Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China Shanxi Key Laboratory of Minor Crop Germplasm Innovation and Molecular Breeding College of Life SciencesShanxi Agricultural UniversityTaigu 030801China State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 310006China

Pre-harvest sprouting(PHS),which reduces grain yield and quality,is controlled by seed dormancy *** few dormancy-related genes have been cloned,the genetic basis of seed dormancy in rice(Oryza sativa L.)remains ***,we performed a genome-wide association study and linkage mapping to dissect the genetic basis of seed dormancy in *** findings suggest that Seed Dormancy4(Sdr4),a central modulator of seed dormancy,integrates the abscisic acid and gibberellic acid signaling pathways at the transcriptional *** analysis revealed that three Sdr4 alleles in rice cultivars already existed in ancestral Oryza rufipogon ***,like the semi-dwarf 1(SD1)and Rc loci,Sdr4 underwent selection during the domestication and improvement of Asian cultivated *** distribution frequency of the Sdr4-n allele in different locations in Asia is negatively associated with local annual temperature and ***,we developed functional molecular markers for Sdr4,SD1,and Rc for use in molecular *** results provide clues about the molecular basis of Sdr4-regulated seed ***,these findings provide guidance for utilizing the favorable alleles of Sdr4 and Rc to synergistically boost PHS resistance,yield,and quality in modern rice varieties.

关键词： climatic adaptation pre-harvest sprouting Rc rice SD1 Sdr4 seed dormancy