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基于METTL3-SFRP4/Wnt/β-catenin通路探讨黄芩清热除痹胶囊改善类风湿性关节炎的机制

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中国中药杂志 2024年第11期49卷 3081-3094页

作者：王玉亭吴雅婕黄玉蓉薛秋云程成龙缪成贵陈光亮安徽中医药大学安徽合肥230012 安徽省中药复方研究重点实验室安徽合肥230012

研究黄芩清热除痹胶囊(Huangqin Qingre Chubi Capsules,HQC)治疗类风湿性关节炎(rheumatoid arthritis,RA)的作用及机制。70只雄性SPF级大鼠随机分为正常组,模型组,HQC低(0.18 g·kg^(-1))、中(0.36 g·kg^(-1))、高(0.72 g·kg^(-1))剂量组,甲氨蝶呤(MTX,0.75 mg·kg^(-1))组,阴性对照组(NC组,模型+生理盐水)。佐剂性关节炎(AA)大鼠成纤维样滑膜细胞(fibroblast-like synoviocytes,FLS)分为正常组,模型组,HQC低(5%HQC含药血清+95%滑膜培养基)、中(10%HQC含药血清+90%滑膜培养基)、高(15%HQC含药血清+85%滑膜培养基)剂量组,阴性对照组。采用实时荧光定量PCR(RT-qPCR)和蛋白免疫印记法(Western blot)检测甲基转移酶3(methyltransgerase like 3,METTL3)、分泌型卷曲相关蛋白4(SFRP4)、β-连环蛋白(β-catenin)、细胞周期蛋白D1(CCND1)、V-Myc骨髓细胞瘤病毒癌基因同源物(c-Myc)、纤连蛋白(fibronectin)、基质金属蛋白酶3(matrix metalloproteinase 3,MMP3)mRNA和蛋白的表达。免疫荧光法进一步检测MMP3和β-catenin蛋白表达。在AA-FLS上敲低METTL3基因表达水平,进一步检测各基因表达情况。酶联免疫吸附法(ELISA)检测白细胞介素(IL)-1β、IL-6、IL-8的水平。结果显示,与正常组相比,模型组大鼠四肢均发现红肿现象,关节肿胀程度明显增加;与模型组相比,各给药组关节肿胀度显著下降(P<0.05),后足撤退阈值和体质量指数均显著增加(P<0.05)。METTL3在AA大鼠高表达,且与SFRP4的表达呈负相关。AA-FLS经给药后,METTL3、β-catenin、CCND1、c-Myc、fibronectin、MMP3 m RNA和蛋白表达显著降低(P<0.05)。与模型组相比,敲低METTL3后β-catenin、CCND1、c-Myc、fibronectin、MMP3 m RNA和蛋白表达均降低(P<0.05)。同时HQC+敲低METTL3组的β-catenin、CCND1、c-Myc、fibronectin、MMP3 m RNA和蛋白表达均明显低于METTL3敲低组(P<0.05)。HQC能不同程度降低IL-1β、IL-6、IL-8水平(P<0.05)。结果表明,HQC对AA大鼠有明显改善作用;METTL3在AA大鼠滑膜组织和AA-FLS中表达显著升高,这可能是诊断和治疗RA的潜在靶点;HQC通过METTL3-SFRP4/Wnt/β-catenin信号通路改善RA,具有明显的抗炎和抗风湿作用。

关键词：类风湿性关节炎黄芩清热除痹胶囊 METTL3 SFRP4 Wnt/β-catenin

Possible shape coexistence in Ne isotopes and the impurity effect of Λ hyperon

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Science China(Physics,Mechanics & Astronomy) 2021年第8期64卷 29-40页

作者： chen chen Qian-Kun Sun yu-Xiao Li Ting-Ting Sun School of Physics and Microelectronics Zhengzhou UniversityZhengzhou 450001China

The possible shape coexistence in even-even Ne isotopes and the impurity effects of the sΛ and pΛ hyperons are explored employing the multidimensionally constrained relativistic-mean-field(MDC-RMF) model with the PK1 parameter set for the N N interaction and PK1-Y1 for the ΛN interaction. The quadrupole deformation potential energy surfaces(PESs), nuclear deformations, nuclear radii, binding energies, and density distributions of the hypernuclei and core nuclei are examined. The possible shape coexistence in ^(24,26,28)Ne is predicted with small energy differences of 140, 336, and 128 keV, respectively, between the two local energy minima. Different impurity effects of the sΛand pΛ hyperons are revealed. The sΛ hyperon exhibits clear shrinkage effects, which reduce the nuclear size and facilitate a spherical nuclear shape. The prolate pΛhyperon on the 1/2^(-)[110] orbital renders the nuclear shape more prolate, while the oblate pΛ hyperon on the 3/2^(-)[101] or 1/2^(-)[101] orbital renders the nuclei more oblate. Moreover, the Λ hyperon can increase the probabilities of the shape coexistence by reducing the energy differences between the two local energy minima, although the shape coexistence may disappear owing to the vanishment of one energy minimum on the flat energy surface.

关键词： single-Λhypernuclei shape coexistence impurity effects Ne isotope

奥希替尼联合贝伐珠单抗治疗EGFR T790M突变晚期肺腺癌的临床观察

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中国处方药 2024年第8期22卷 8-11页

作者：王雨婷陈凯天津市第四中心医院肿瘤科天津300140

目的探讨表皮生长因子受体(EGFR)T790M突变晚期肺腺癌患者应用奥希替尼(AZD9291)、贝伐珠单抗联合治疗的临床疗效。方法选取2020年1月~2023年6月接受治疗的经病理及基因检测证实EGFR T790M突变晚期肺腺癌患者96例进行研究。使用动态随... 详细信息

目的探讨表皮生长因子受体(EGFR)T790M突变晚期肺腺癌患者应用奥希替尼(AZD9291)、贝伐珠单抗联合治疗的临床疗效。方法选取2020年1月~2023年6月接受治疗的经病理及基因检测证实EGFR T790M突变晚期肺腺癌患者96例进行研究。使用动态随机法将上述患者分为对照组(n=48)与观察组(n=48)。对照组给予奥希替尼治疗,观察组给予贝伐珠单抗联合奥希替尼治疗。比较两组近期疗效、远期疗效、血清指标[糖类抗原125(CA125)、癌胚抗原(CEA)、血管内皮生长因子(VEGF)]以及不良反应。结果观察组的近期疗效显著优于对照组(P0.05)。结论对于EGFR T790M突变晚期肺腺癌患者,使用贝伐珠单抗联合奥希替尼治疗可提高近、远期疗效,降低肿瘤标志物表达水平,且不会增加不良反应的发生率。

关键词：表皮生长因子受体奥希替尼肺腺癌贝伐珠单抗

不同品种高油酸花生热风干燥特性及热力学性质研究

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食品工业科技 2024年第14期45卷 42-49页

作者：李玉朱文学陈鹏枭王延坤蒋萌蒙王殿轩陈亮张昱泉胡新阳王瑾河南工业大学粮食和物资储备学院河南郑州450001 河南工业大学生物工程学院河南郑州450001 河北省农林科学院粮油作物研究所河北石家庄050035

为探究高油酸花生干燥过程中的水分变化规律,本文研究了不同风温(35、40、45、50℃)、风速(0.5、0.8、1.1 m/s)对三种不同高油酸花生热风干燥特性的影响,并借助Weibull分布函数分析干燥过程。结果表明,高油酸花生干燥过程以降速干燥为主... 详细信息

为探究高油酸花生干燥过程中的水分变化规律,本文研究了不同风温(35、40、45、50℃)、风速(0.5、0.8、1.1 m/s)对三种不同高油酸花生热风干燥特性的影响,并借助Weibull分布函数分析干燥过程。结果表明,高油酸花生干燥过程以降速干燥为主;风温、风速越高,高油酸花生有效水分扩散系数越大、干燥速率越快,干燥用时越短。其中,‘圣油3号’花生水分扩散速率最快、活化能最低,与Weibull分布函数拟合结果最佳;尺度参数α值随风温、风速的升高而降低,风温对α值影响极显著(P<0.01),风速对α值影响显著(P<0.05);干燥条件对形状参数β值无显著影响。另外,温度的升高促进了焓值和熵值的降低和吉布斯自由能的增加,表明干燥过程需要从干燥介质中吸收能量方能进行。Weibull分布函数可用于描述高油酸花生的热风干燥过程,为高油酸花生的工业化干燥提供技术依据,奠定理论基础。

关键词：高油酸花生热风干燥 Weibull 分布函数热力学性质

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An assessment of “fishing down marine food webs” in coastal states during 1950–2010

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Acta Oceanologica Sinica 2017年第2期36卷 43-50页

作者： DING Qi chen Xinjun yu Wei chen Yong College of Marine Sciences Shanghai Ocean University Shanghai 201306 China Key Laboratory of Sustainable Exploitation of Oceanic Fisheries Resources of Ministry of Education Shanghai Ocean University Shanghai 201306 China National Engineering Research Center for Oceanic Fisheries Shanghai Ocean University Shanghai 201306 China Collaborative Innovation Center for Distant-water Fisheries Shanghai 201306 China School of Marine Sciences University of Maine Orono Maine 04469 USA

Mean trophic level of fishery landings（MTL） is one of the most widely used biodiversity indicators to assess the impacts of fishing. Based on the landing data compiled by Food and Agriculture Organization combined with trophic information of relevant species in Fish Base, we evaluated the status of marine fisheries from 1950 to 2010 for different coastal states in Pacific, Atlantic and Indian Oceans. We found that the phenomenon of ＂fishing down marine food webs＂ occurred in 43 states. Specifically, 27 states belonged to ＂fishing-through＂ pattern, and 16 states resulted from ＂fishing-down＂ scenario. The sign of recovery in MTL was common in the Pacific, Atlantic and Indian Oceans（occurred in 20 states）, but was generally accompanied by significantly decreased catches of traditional low trophic level species. In particular, 11 states showed significant declining catches of lower trophic levels. The MTL-based assessment of ＂fishing down marine food webs＂ needs to be interpreted cautiously.

关键词： coastal states exploitation history fishing down marine food webs sustainability marine fisheries mean trophic level of fishery landings

Light-Regulated Stomatal Aperture in Arabidopsis

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Molecular Plant 2012年第3期5卷 566-572页

作者： chen chen yu-Guo Xiao Xin Li in Ni Department of Plant Biology University of Minnesota Saint Paul MN 55108 USA

The stomatal pores of plant leaves, situated in the epidermis and surrounded by a pair of guard cells, allow CO2 uptake for photosynthesis and water loss through transpiration. Blue light is one of the dominant environmental signals that control stomatal movements in leaves of plants in a natural environment. This blue light response is mediated by blue/UV A light-absorbing phototropins （phots） and cryptochromes （crys）. Red/far-red light-absorbing phytochromes （phys） also play a role in the control of stomatal aperture. The signaling components that link the perception of light signals to the stomatal opening response are largely unknown. This review discusses a few newly discovered nuclear genes, their function with respect to the phot-, cry-, and phy-mediated signal transduction cascades, and possible involve- ment of circadian clock.

关键词： circadian clock cryptochromes phototropins phytochromes light signaling stomata

Some notes on numerical simulation and error analyses of the attached turbulent cavitating flow by LES

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Journal of Hydrodynamics 2018年第2期30卷 369-372页

作者： Xin-ping Long yun Long Wen-ting Wang huai-yu cheng Bin Ji State Key Laboratory of Water Resources and Hydropower Engineering Science School of Power and Mechanical Engineering Wuhan University Wuhan 430072 China Key Laboratory of Jet Theory and New Technology of Hubei Province Wuhan University Wuhan 430072 China

In this letter, the attached turbulent cavitating flow around the Clark-Y hydrofoil is investigated by the numerical simulation with special emphasis on error analysis of large eddy simulation（LES） for the unsteady cavitation simulation. The numerical results indicate that the present simulation can capture the periodic cavity shedding behavior and show a fairly good agreement with the available experimental data. Further analysis demonstrates that the cavitation has a great influence on LES numerical error and modeling error. The modeling error and numerical error are almost on the same order of magnitude, while the modeling error often shows a little bit larger magnitude than numerical error. The numerical error and modeling error sometimes can partially offset each other if they have the opposite sign. Besides, our results show that cavitation can extend the magnitudes and oscillation levels of numerical error and modeling error.

关键词： Cavitation cavitating flow large eddy simulation (LES) error analyses verification and validation (V&V)

Distance Estimates for Simple Choreographic Solutions of the Three-body Problem

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Acta Mathematica Sinica,English Series 2022年第10期38卷 1823-1830页

作者： Kuo Chang chen Bo yu PAN Department of Mathematics “National Tsing Hua University”HsinchuTaiwanChina Department of Applied Mathematics “National Chung Hsing University”TaichungTaiwanChina

In this article we estimate mutual distances of simple choreographic solutions for the Newtonian three-body *** formulas will be proved and our applications include the famous figure-8 orbit.

关键词： n-body problem variational method mutual distance

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脑梗死后头痛发生机制及治疗进展

中国实用医药 2024年第17期19卷 173-176页

作者：陈俊宇马可广西钦州市第一人民医院神经内科 535099

脑梗死(CI)又称为缺血性脑卒中,在所有脑卒中中占比60.00%~80.00%;脑出血称为出血性脑卒中,在所有脑卒中中占比20.00%~30.00%。在2016年全世界由于脑血管疾病造成死亡的人数约550万人,高居死亡病因首位。同时脑梗死在一定程度上给存活... 详细信息

脑梗死(CI)又称为缺血性脑卒中,在所有脑卒中中占比60.00%~80.00%;脑出血称为出血性脑卒中,在所有脑卒中中占比20.00%~30.00%。在2016年全世界由于脑血管疾病造成死亡的人数约550万人,高居死亡病因首位。同时脑梗死在一定程度上给存活者遗留认知功能障碍、语言功能障碍、肢体瘫痪以及疼痛等多种后遗症,对脑梗死患者的生活质量造成严重影响,其中脑梗死后头痛是最常见的临床症状之一。因此,本文针对脑梗死后头痛发生机制以及治疗进展进行综述,以期为临床诊疗提供参考依据。

关键词：脑梗死头痛缺血性脑卒中发病机制