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Oncogenic potential of IDH1R132C mutant incholangiocarcinoma development in mice

World Journal of Gastroenterology 2016年第6期22卷 2071-2080页

作者： Ning Ding li che Xiao-Lei li Yan liu li-Jie Jiang Biao Fan Jun-Yan Tao Xin chen Jia-Fu Ji Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education) Department of Gastrointestinal SurgeryPeking University Cancer Hospital and InstituteBeijing 100142China Department of Bioengineering and Therapeutic Sciences Liver CenterUniversity of CaliforniaSan FranciscoCA 94143United States Department of Hepatobiliary Surgery Xijing HospitalThe Fourth Military Medical UniversityXi’an 710032Shanxi ProvinceChina School of Pharmacy Hubei University of Chinese MedicineWuhan 430065Hubei ProvinceChina

AIM: To investigate whether IDH1R132 C mutant in combination with loss of p53 and activated Notch signaling promotes intrahepatic cholangiocarcinoma(ICC) ***: We applied hydrodynamic injection and sleeping beauty mediated somatic integration to induce loss of p53(via sh P53), activation of Notch [via intracellular domain of Notch1(NICD)] and/or overexpression of IDH1R132 C mutant together with the sleeping beauty transposase into the mouse liver. Specifically, we co-expressed sh P53 and NICD(sh P53/NICD, n = 4), sh P53 and IDH1R132C(sh P53/IDH1R132 C, n = 3), NICD and IDH1R132C(NICD/IDH1R132 C, n = 4), as well as NICD, sh P53 and IDH1R132C(NICD/sh P53/IDH1R132 C, n = 9) in mice. Mice were monitored for liver tumor development and euthanized at various time points. liver histology was analyzed by hematoxylin and eosin staining. Molecular features of NICD/sh P53/IDH1R132 C ICC tumor cells were characterized by Myc tag, Flag tag, Ki-67, p-Erk and p-AKT immunohistochemical staining. Desmoplastic reaction in tumor tissues was studied by Picro-Sirius red ***: We found that co-expression of sh P53/NICD, sh P53/IDH1R132 C or NICD/IDH1R132 C did not lead to liver tumor formation. In striking contrast, coexpression of NICD/sh P53/IDH1R132 C resulted in ICC development in mice(P liver surface. NICD/sh P53/IDH1R132 C liver tumors shared multiple histological features of human ICCs, including hyperplasia of irregular glands. Importantly, all tumor cells were positive for the biliary epithelial cell marker cytokeratin 19. Extensive collagen fibers could be visualized in tumor tissues using Sirus red staining, duplicating the desmoplastic reaction observed in human ICC. Tumors were highly proliferative and expressed ectopically injected genes. Toget

关键词： IDH1 mutant Notch pathway Intrahepaticcholangiocarcinoma Mouse liver cancer p53

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Retrospective Study of Effect of Fine Particulate Matter on Acute Exacerbation of Patients with Idiopathic Pulmonary Fibrosis

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Biomedical and Environmental Sciences 2020年第2期33卷 138-140页

作者： che Chun li DONG Fu Shi li Jia lin ZHANG Chun ling liU Lu WU Yu Han WANG Kun QI Hong Department of Respiratory Medicine First Affiliated Hospital of Harbin Medical UniversityHarbin 150001HeilongjiangChina State Key Lab of Urban Water Resource and Environment Harbin Institute of TechnologyHarbin 150090HeilongjiangChina

Acute exacerbation of idiopathic pulmonary fibrosis(AEIPF) is the most important cause of health deterioration and death as a result of idiopathic pulmonary fibrosis(IPF).In recent years,the hospitalization and mortality rates due to AEIPF have increased significantly[1].However,until the present,the cause of AEIPF remains unclear,thereby needing research attention.

关键词： Retrospective Study Fibrosis

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Activity and Adsorption Behavior of Oxygen on Rutile TiO_(2)(110)

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Chinese Journal of chemical Physics 2024年第5期37卷 619-626,I0051-I0058,I0100页

作者： Rulin Sun Xinlu liu Fangliang li li che College of Environmental Sciences and Engineering Dalian Maritime UniversityDalian 116026China State Key Laboratory of Molecular Reaction Dynamics Dalian Institute of Chemical PhysicsDalian 116023China School of Science Dalian Maritime UniversityDalian 116026China

The activity and adsorption behavior of oxygen on rutile TiO_(2)(110)(RTiO_(2)(110))were investigated using the temperature programmed desorption(TPD)method with methanol(CH_(3)OH)as the probe *** controlling the coverage of molecular O_(2)on the surface via increasing or decreasing O_(2)exposure,two chemisorbed O_(2)species on the surface are confirmed,one at the bridging oxygen vacancy(Ov)site(O_(2)^(2-)/Ov)and the other at the five-fold coordinated titanium(Ti_(5c))site(O^(2-)/Ti_(5c)).At low O_(2)exposure,O^(2-)/Ov is the main species on the surface,which only leads to the O-H bond cleavage of CH_(3)OH,producing methoxy groups(CHgO).However,after the Ov sites are nearly filled by O_(2)at about 0.1 L O_(2)exposure,O_(2)/Tisc species begins to appear on R-TiO_(2)(110)surface,resulting in the formation of formaldehyde(CH_(2)O)via the reaction of O_(2)/Tisc species with CH_(3)OH or CH3O to break the C-H bond at low surface ***,the yield of CH_(2)O increases linearly with that of H_(2)*** addition,when the 1 L O_(2)covered surface is irradiated with 355 nm UV irradiation to desorb and dissociate O_(2)/Ti_(5c)species,the yield of CH_(2)O decreases linearly with that of H_(2)*** analysis suggests that the charge state of O_(2)/Ti_(5c)may not change as the exposure of O_(2)changes on the R-TiO_(2)(110)surface,and O_(2)is most likely to adsorb on the Ti_(5c)sites in the form of O_(2)^(2-),not O_(2)^(-),The result not only advances our understanding on the adsorption state of O_(2)on TiO_(2),but also provides clues for low temperature C-H bond activation with O_(2)on TiO_(2).

关键词： Rutile TiO_(2)(110) Methanol Oxygen Temperature programmed desorption

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The Vigorous, Forceful, Extensive and Profound Style of Calligraphy——Kang Wanli, Inheritance of Beipai Kangti Calligraphy

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科技视界 2015年第10期 150-151页

作者： che Wan-li Qingdao University Music Department

中国书法家康万里以其粗狂、豪放、厚重、泼辣的书写风格确立了享誉海内外的北派康体书法。本文回顾了康万里成长经历和艺术成就,真实呈现了康万里在推进中国书法艺术事业中所起到的积极努力。笔者希望通过此文能让更多的人了解北派康... 详细信息

中国书法家康万里以其粗狂、豪放、厚重、泼辣的书写风格确立了享誉海内外的北派康体书法。本文回顾了康万里成长经历和艺术成就,真实呈现了康万里在推进中国书法艺术事业中所起到的积极努力。笔者希望通过此文能让更多的人了解北派康体书法。

关键词：康万里北派康体书法书法家艺术成就

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Expression of HSV-1 ICP0 Antigen Peptide in Prokaryotic Cells and Preparation of Specific Antibody

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中国病毒学 2007年第4期22卷 280-286页

作者： Wei-zhong li Wei CUN Long-ding liU Yan-chun che Jie LUO li-chun WANG cheng-hong DONG Qian YANG Qi-han li Institute of Medical Biology Chinese Academy of Medical Sciences and Peking Union Medical College Kunming Yunnan Province 650118 China

As an immediate-early protein of herpes simplex virus, infected-cell polypeptide 0 (ICP0) exhibits complicated interactions with host cells, and its regulatory function on gene expression is of great importance. Since the ICP0 encoding sequence contains many rare codons which are absent in ***, and ICP0 is highly unstable in prokaryotic cells, expression of entire ICP0 in prokaryotic cells has never been reported. In order to further investigate the function of ICP0, a recombinant plasmid was constructed by subcloning a cDNA fragment encoding an amino-terminal of 105 residues of the ICP0 protein into pGEX-5x-1 vector. The resulting GST-105 fusion antigen peptide was expressed with high efficiency in ***. Antibodies prepared after the immunization of mice with purified fusion protein can recognize not only the denatured ICP0 protein, but also the native ICP0 protein with normal biological conformation.

关键词： ICPO 抗原肽原核细胞抗体单纯疱疹病毒基因表达

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How to rehabilitate Olympic class athletes

How to rehabilitate Olympic class athletes

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中华医学会第八届骨科学术会议暨第一届国际COA学术大会

作者： Raymond che Tin li

＜正＞Rehabilitation by definition is the process of restoration of skills by a person who has had an illness or injury so as to regain maximum self-sufficiency and function in a normal or as near normal manner as possible. More academically, it refers to the process of applying stress to healing tissue in accordance with the specific stresses that it will face upon

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Mobilization of Peripheral Blood Stem Cells Using Regimen Combining Docetaxel with Granulocyte Colony-stimulating Factor in Breast Cancer Patients

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Chinese Journal of Cancer Research 2011年第1期23卷 49-53页

作者： Jing Yu Jun Ren li-jun Di Guo-hong Song Yu-lin Zhu Jie Zhang Xu liang li che Han-fang Jiang Jun Jia Chun-rong Zhang Key laboratory of Carcinogenesis and Translational Research(Ministry of Education) Department of Breast OncologyBeijing 100142China Key laboratory of Carcinogenesis and Translational Research (Ministry of Education) Department of Transfusion Peking University School of Oncology Beijing Cancer Hospital ＆ Institute Beijing 100142 China

Objective：To evaluate the effectiveness and safety of the mobilization of peripheral blood hematopoietic stem cells by combining docetaxel with granulocyte colony-stimulating factor（G-CSF） in breast cancer ***：A total of 57 breast cancer patients were treated with docetaxel 120 mg/*** the white blood cell（WBC） count decreased to 1.0×109/L,patients were given G-CSF 5-g/kg daily by subcutaneous injection until the end of *** blood mononuclear cells（MNC） were isolated by Cobe Spectra Apheresis *** percentage of CD34＋ cell was assayed by flow ***：At a median 6 of days（range 3-8） after the administration of docetaxel,the median WBC count decreased to 1.08×109/L（range 0.20-2.31）.The median duration of G-CSF mobilization was 3 days（range 2-7）.The MNC collection was conducted 8-12 days（median 10 days） after docetaxel *** median MNC was 5.35×108/kg（range 0.59-14.07）,the median CD34＋ cell count was 2.43×106/kg（range 0.16-16.69）.The CD34＋ cell count was higher than 1.00×106/kg in 47 of 57 cases（82.46%） and higher than 2.00×106/kg in 36 cases（63.16%）.The CD34＋ cell count was higher than 2.00×106/kg in 27 collections（23.68%）.The MNC count and the CD34＋ cell count were correlated with the bottom of WBC after docetaxel chemotherapy（r=0.364,0.502,P=0.005,0.000）.The CD34＋ cell count was correlated with the MNC count（r=0.597,P=0.000）.The mobilization and apheresis were well tolerated in all *** perioral numbness and numbness of hand or feet were observed in 3 *** serious adverse events were ***：Mobilization of peripheral blood hematopoietic stem cell by combining docetaxel with G-CSF was effective and safety in breast cancer patients.

关键词： Docetaxel Granulocyte colony-stimulating factor(G-CSF) Mobilization Stem cell

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“热证可灸”的理论探讨与临证应用

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中国针灸 2005年第10期25卷 715-716页

作者：车建丽苏州大学附属第一医院针灸科江苏215006

探讨针灸临床“热证是否宜灸”的疑难问题。对针灸经典专著的有关篇章加以引用、分析,结合笔者多年来的实践经验以及现代医学的实验研究。考证了“正反逆从”的治疗原则及“以热引热”的治疗方法为“热证可灸”的最朴素的理论依据,艾灸... 详细信息

探讨针灸临床“热证是否宜灸”的疑难问题。对针灸经典专著的有关篇章加以引用、分析,结合笔者多年来的实践经验以及现代医学的实验研究。考证了“正反逆从”的治疗原则及“以热引热”的治疗方法为“热证可灸”的最朴素的理论依据,艾灸的抑菌、退热、改善微循环等作用机理也为实验研究所证实。说明艾灸适用于包括实热和虚热在内的所有热证。

关键词：热证/针灸疗法针灸禁忌灸法反治

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基于时分MIMO的地基雷达高分辨率成像研究

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电子与信息学报 2016年第5期38卷 1055-1063页

作者：蒋留兵杨涛车俐桂林电子科技大学广西无线宽带通信与信号处理重点实验室桂林541004 桂林电子科技大学信息与通信学院桂林541004

基于时分多输入多输出(MIMO)的地基雷达成像有许多很好的应用价值,比如替代合成孔径雷达成像在山体滑坡监测方面的应用。针对基于时分MIMO的地基雷达高效高分辨率成像,该文提出一种基于逆傅里叶变换脉冲压缩和波束形成的成像算法。雷达... 详细信息

基于时分多输入多输出(MIMO)的地基雷达成像有许多很好的应用价值,比如替代合成孔径雷达成像在山体滑坡监测方面的应用。针对基于时分MIMO的地基雷达高效高分辨率成像,该文提出一种基于逆傅里叶变换脉冲压缩和波束形成的成像算法。雷达通过步进频连续波技术获得高距离向分辨率,利用MIMO技术获得高方位向分辨率。通过逆傅里叶变换法实现雷达数据距离向压缩,采用波束形成算法实现雷达数据方位向压缩。同时该算法还针对MIMO天线阵列所引起的回波信号相位不连续问题进行了适当的校正,在保持算法高效性的同时提高了成像质量。根据真实的山体滑坡监测成像场景参数,通过数值仿真验证了该成像算法的可行性,应用在山体滑坡监测上理论效果良好。

关键词：雷达成像时分MIMO雷达高分辨率波束形成逆傅里叶变换脉冲压缩

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周围性面神经炎急性期梅花针叩刺的疗效观察

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中国针灸 2005年第12期25卷 851-852页

作者：车建丽苏州大学附属第一医院针灸科江苏215006

目的比较梅花针叩刺与激素疗法对急性期周围性面神经炎的疗效差异。方法治疗组32例急性期用梅花针叩刺治疗,对照组30例口服或静脉点滴地塞米松,治疗5天后2组均只用针刺治疗。对比两组疗效。结果治疗组总有效率为100.0%,对照组总有效率为... 详细信息

目的比较梅花针叩刺与激素疗法对急性期周围性面神经炎的疗效差异。方法治疗组32例急性期用梅花针叩刺治疗,对照组30例口服或静脉点滴地塞米松,治疗5天后2组均只用针刺治疗。对比两组疗效。结果治疗组总有效率为100.0%,对照组总有效率为93.4%,经统计学χ2检验,P<0.05,二者差异有显著性意义。结论急性期梅花针叩刺治疗面神经炎疗效优于激素治疗。

关键词：面神经麻痹/针灸疗法急性病梅花针疗法

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