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基于教师资格证国考背景下体育师范生综合素质提升路径研究

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当代体育科技 2024年第25期14卷 104-109页

作者：姜定波常丽娟莫琰六盘水师范学院体育学院贵州六盘水553000

该文利用文献资料、访谈、实地调查、数理统计等方法对地方院校体育师范专业学生综合素质能力进行深入调查,发现学校体育师范生存在主动性态度较差、团结意识有待提升、部分学生学习目标不明确、专业理论知识深度较差和自身的就业目标... 详细信息

该文利用文献资料、访谈、实地调查、数理统计等方法对地方院校体育师范专业学生综合素质能力进行深入调查,发现学校体育师范生存在主动性态度较差、团结意识有待提升、部分学生学习目标不明确、专业理论知识深度较差和自身的就业目标不清晰等问题。因此,需跟紧社会发展需求,加强实习实训基地拓展,提升学生实践能力;结合社会市场及用人单位反馈,不断修订人才培养方案,强化培养质量;强化开学“第一课”的重要性,塑造学生良好价值观;辅导员、班主任和专任教师需要充分发挥工作职责,为学生质量、就业提供保障;双导师制必须强有力落实,必须充分发挥教师作用.为落实立德树人根本任务,全面推动体育师范生综合素质的提升,提高体育师范生社会竞争力奠定基础。

关键词：教师资格证体育师范生综合素质提升路径

Molecular characterization of GmNHX2,a Na^+/H^+ antiporter gene homolog from soybean,and its heterologous expression to improve salt tolerance in Arabidopsis

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Chinese Science Bulletin 2009年第19期54卷 3536-3545页

作者： ZHOU GuoAn GUAN RongXia LI YingHui chang RuZhen QIU lijuan National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)/Key Lab of Germplasm & Biotechnology (MOA) Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing 100081 China

Na+/H+ antiporters have been well documented to enhance plant salt tolerance by regulating cellular ion homeostasis. Here, a putative Na+/H+ antiporter gene homolog GmNHX2 from soybean was cloned and predicted to encode a protein of 534 amino acids with 10 putative transmembrane domains. GmNHX2 was expressed in all soybean plant tissues but enriched in roots and its expression was induced by NaCl and polyethylene glycol (PEG) treatments. GmNHX2 exhibits greater sequence similarity with LeNHX2 and AtNHX6 than that of AtNHX1 and AtSOS1. Although phylogenetic analysis clustered GmNHX2 with organellar (tonoplast and vesicles) antiporters, the GmNHX2-EGFP (enhanced green fluorescent protein) fusion protein was possibly localized in the plasma membrane or organelle membrane of transgenic plant cells. Furthermore, transgenic Arabidopsis plants expressing GmNHX2 were more tolerant to high NaCl concentrations during germination and seedling stages when compared with wild-type plants. These results suggest that GmNHX2 is a membrane Na+/H+ antiporter and may function to regulate ion homeostasis under salt stress.

关键词：植物耐盐基因工程盐胁迫离子分布植物组织 GmNHX2

Boruta-Optuna-XGBoost融合模型的聚驱油田产量智能预测方法研究

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重庆理工大学学报（自然科学） 2024年第4期38卷 154-160页

作者：田枫曹凯光赵玲张孟阳刘芳苏若禹常丽娟东北石油大学计算机与信息技术学院黑龙江大庆163318

在聚驱油田开采时,存在向油层中加注水溶性高分子聚合物的过程,此过程包含大量参数,对油田产量具有较大影响。针对传统油田产量预测方法存在的人工计算量大、准确率低的问题。提出一种Boruta-Optuna-XGBoost融合模型对聚驱油田产量进行... 详细信息

在聚驱油田开采时,存在向油层中加注水溶性高分子聚合物的过程,此过程包含大量参数,对油田产量具有较大影响。针对传统油田产量预测方法存在的人工计算量大、准确率低的问题。提出一种Boruta-Optuna-XGBoost融合模型对聚驱油田产量进行预测,解决传统方法存在的问题。通过Boruta特征筛选方法进行聚驱油田特征筛选,降低特征冗余,提高特征相关性,避免模型过拟合;使用Optuna超参数优化算法对XGBoost进行自适应超参数评价,提高模型精度;使用最优超参的XGBoost算法对聚驱油田产量进行回归预测,通过算法建立油田注入参数和油田月产量之间的逻辑关系模型,对聚驱油田的月产量进行预测。所提方法应用在大庆油田的实际有效数据的准确率达95%,证明了方法的有效性,能够对油田的生产效益、资源配置和可持续发展产生影响,也为数字化聚驱油田智能产量预测发展提供了新思路。

关键词：聚驱油田产量预测特征筛选超参评价 XGBoost

Optimized Synthesis of Carbon Aerogels via Ambient Pressure Drying Process as Electrode for Supercapacitors

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Journal of Wuhan University of Technology(Materials Science) 2015年第6期30卷 1325-1331页

作者：袁磊 chang lijuan FU Zhibing YANG Xi JIAO Xingli TANG Yongjian LIU Xichuan 王朝阳 Science and Technology on Plasma Physics Laboratory Research Centre of Laser Fusion China Academy of Engineering Physics Institute of Atomic and Molecular Physics Sichuan University College of Material Science and Engineering Xihua University

Carbon aerogels were synthesized via ambient pressure drying process using resorcinolformaldehyde as precursor and P123 to strengthen their skeletons. CO2 activation technology was implemented to improve surface areas and adjust pore size distribution. The synthesis process was optimized, and the morphology, structure, adsorption properties and electrochemical behavior of different samples were characterized. The CO2-activated samples achieved a high specific capacitance of 129.2 F/g in 6 M KOH electrolytes at the current density of 1 m A/cm^2 within the voltage range of 0-0.8 V. The optimized activation temperature and duration were determined to be 950 ℃ and 4 h, respectively.

关键词： supercapacitors carbon aerogels ambient pressure drying process activation

职场适应在规范化培训护士逆境商与主动职业行为之间的中介效应

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中国社会医学杂志 2024年第2期41卷 195-199页

作者：孙宇周祥常丽娟彭守华张庆庆新疆维吾尔自治区人民医院新疆乌鲁木齐830001 新疆医科大学附属肿瘤医院新疆乌鲁木齐830000

目的探讨规范化培训护士逆境商与主动职业行为的关系,以及职场适应的中介作用。方法采取便利抽样法,于2021年12月-2022年3月,采用一般资料调查表、职场适应量表、逆境商量表、主动职业行为量表对新疆5家三级医院486名规范化培训护士进... 详细信息

目的探讨规范化培训护士逆境商与主动职业行为的关系,以及职场适应的中介作用。方法采取便利抽样法,于2021年12月-2022年3月,采用一般资料调查表、职场适应量表、逆境商量表、主动职业行为量表对新疆5家三级医院486名规范化培训护士进行问卷调查。结果规范化培训护士逆境商、职场适应、主动职业行为得分分别为(121.72±17.59)分、(58.91±9.17)分、(42.17±9.84)分,职场适应、逆境商、主动职业行为均呈显著正相关(r=0.561~0.707,P<0.001);职场适应在逆境商与主动职业行为之间起部分中介作用(效应值为0.21,95%CI:0.12~0.27)。结论规范化培训护士逆境商、职场适应、主动职业行为均处于中等水平,逆境商不仅可以直接影响主动职业行为,还可以通过职场适应的中介作用对主动职业行为产生间接影响。建议管理者对规培护士进行逆商教育,通过正确的心理培训改善其应对挫折的能力,提高职场适应力,从而提高其主动职业行为水平。

关键词：职场适应规范化培训护士逆境商主动职业行为中介效应

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A conserved unusual posttranscriptional processing mediated by short,direct repeated (SDR) sequences in plants

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Journal of Genetics and Genomics 2010年第1期37卷 85-99页

作者： Xiangli Niu Di Luo Shaopei Gao Guangjun Ren lijuan chang Yuke Zhou Xiaoli Luo Yuxiang Li Pei Hou Wei Tang Bao-Rong Lu Yongsheng Liu College of Agricultural and Life Sciences Chongqing UniversityChongqing 400030China Ministry of Education Key Laboratory for Bio-resource and Eco-environment College of Life Science and State Key Laboratory of BiotherapySichuan UniversityChengdu 610064China c Institute of Crop Research Sichuan Academy of Agricultural SciencesChengdu 610066China Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering Institute of Biodiversity ScienceFudan UniversityShanghai 200433China1

In several stress responsive gene loci of monocot cereal crops,we have previously identified an unusual posttranscriptional processing mediated by paired presence of short direct repeated （SDR） sequences at 5＇ and 3＇ splicing junctions that are distinct from conventional （U2/U12-type） splicing *** using the known SDR-containing sequences as probes,24 plant candidate genes involved in diverse functional pathways from both monocots and dicots that potentially possess SDR-mediated posttranscriptional processing were predicted in the GenBank *** SDRs-mediated posttranscriptional processing events including cis-and trans-actions were experimentally detected in majority of the predicted *** sequence analysis demonstrates several types of SDR-associated splicing peculiarities including partial exon deletion,exon fragment repetition,exon fragment scrambling and trans-splicing that result in either loss of partial exon or unusual exonic sequence rearrangements within or between RNA *** addition,we show that the paired presence of SDR is necessary but not sufficient in SDR-mediated splicing in transient expression and stable transformation *** also show prokaryote is incapable of SDR-mediated premRNA splicing.

关键词： posttranscriptional processing short direct repeat (SDR) premRNA splicing plant

Effects of Severe Drought and Glyphosate Stress on Physiological Characteristics and Protein Expression of Photosystem Ⅱ in Genentically Modified Soybean

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Plant Diseases and Pests 2019年第2期10卷 22-26页

作者： chang lijuan Liu Wenjuan Zhang Fuli Analysis and Test Center Sichuan Academy of Agricultural Sciences

[Objective] The paper was to investigate effects of glyphosate stress on physiological characteristics and protein expression of photosystem Ⅱ(PSⅡ) in genentically modified soybean GTS 40-3-2 seedlings under severe drought condition. [Method] A pot experiment was carried out in growth chamber to determine the response of genetically modified soybean treated by severe drought stress and different concentrations of glyphosate at the third compound leaf stage. [Result] Severe drought treatment increased the electrolyte leakage(EL), superoxide dismutase(SOD) and peroxidase(POD) activities, and decreased the relative water content(RWC), chlorophyll content, and catalase(CAT) activity. The EL, SOD and POD activities were significantly increased in severe drought and glyphosate treatments, which were related to glyphosate concentrations. The chlorophyll content decreased, which was also related to glyphosate concentrations. But the BWC and CAT activity were not affected by glyphosate concentrations. Western blot displayed that PSⅡ protein Lhcb2 was not affected by stress conditions and stably expressed. D1, D2 and Lhcb4 protein level decreased, and there was no significant change in Lhcb1 expression under severe drought stress. The protein levels of D1, D2, Lhcb1 and Lhcb4 decreased with the increase of glyphosate concentrations under severe drought and glyphosate stress. When the glyphosate concentrations were 0.92 and 1.84 kg·ai/hm^2, the protein levels of D1, D2 and Lhcb4 were slightly higher than those in severe drought stress. When the glyphosate concentrations were 3.68 and 7.36 kg·ai/hm^2, the protein level of D1, D2, Lhcb1 and Lhcb4 decreased sharply. [Conclusion] This research provides a theoretical basis for production of genetically modified soybean.

关键词： Severe drought stress Glayphosate Genetically modified soybean Photosystem Ⅱ

模拟增温对管涔山林下草地土壤可培养细菌数量的影响

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防护林科技 2024年第1期 16-20,24页

作者：吴佳灵刘晓娇常丽娟朱茜冯煜清郭子怡赵慧茹张宝贵太原师范学院地理科学学院山西晋中030619 中国科学院西北生态环境资源研究院甘肃省极端环境微生物资源与工程重点实验室甘肃兰州730000

作为生态系统的重要组成部分,土壤微生物对温度变化十分敏感。为揭示气候变暖对管涔山不同海拔(2222.4、2395.0和2571.3 m)土壤细菌数量变化特征的影响,采用开顶式增温箱进行模拟增温试验,设置CK(对照)、低度增温、高度增温3种处理,采... 详细信息

作为生态系统的重要组成部分,土壤微生物对温度变化十分敏感。为揭示气候变暖对管涔山不同海拔(2222.4、2395.0和2571.3 m)土壤细菌数量变化特征的影响,采用开顶式增温箱进行模拟增温试验,设置CK(对照)、低度增温、高度增温3种处理,采用可培养方法研究不同增温处理下土壤细菌数量特征,同时测定理化性质,并分析其相关性。结果表明:(1)与对照相比,3个海拔样地2种增温处理均显著提高了土壤可培养细菌数量(P<0.05)。(2)不同海拔下,2种增温处理细菌数量的变化特征不同,只有在2571.3 m处,高度增温显著高于低度增温(P<0.01)。(3)纵向来看,CK与低度增温处理样地细菌数量随海拔升高呈显著下降趋势(P<0.05),而高度增温样地呈相反趋势,但未达到显著水平。(4)研究区可培养细菌数量与含水量呈极显著正相关关系(P<0.01)。研究结果为区域生态系统微生物响应气候变暖提供理论依据与数据支撑。

关键词：管涔山模拟增温细菌数量环境因子

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五台山不同海拔土壤可培养细菌数量特征研究

防护林科技 2024年第1期 61-65,68页

作者：常丽娟刘晓娇吴佳灵朱茜冯煜清赵慧茹郭子怡张宝贵太原师范学院地理科学学院山西晋中030619 中国科学院西北生态环境资源研究院甘肃省极端环境微生物资源与工程重点实验室甘肃兰州730000

为探究山西境内不同海拔梯度下土壤可培养细菌数量特征及其变化趋势,选择海拔梯度大、垂直特征明显的五台山作为研究区域,以不同海拔(1452、1646、1870、2068、2284、2522和2750 m)、不同深度(0~10 cm、20~30 cm)土壤为研究对象,采用稀... 详细信息

为探究山西境内不同海拔梯度下土壤可培养细菌数量特征及其变化趋势,选择海拔梯度大、垂直特征明显的五台山作为研究区域,以不同海拔(1452、1646、1870、2068、2284、2522和2750 m)、不同深度(0~10 cm、20~30 cm)土壤为研究对象,采用稀释平板法分析不同海拔、不同土壤深度可培养细菌数量分布特征。结果表明:五台山土壤可培养细菌数量介于4.30×10^(7)~18.55×10^(7)CFU·g^(-1)之间,平均值为10.80×10^(7)CFU·g^(-1);可培养细菌数量随着海拔高度的上升呈显著下降趋势(P<0.05);除1646 m样点外,其他6个样点0~10 cm与20~30 cm土壤可培养细菌数量均存在显著差异(P<0.05);土壤可培养细菌数量与含水量呈极显著负相关(P<0.01)。研究区域土壤可培养细菌数量受海拔高度、土壤深度及含水量显著影响。

关键词：五台山海拔土壤细菌数量变化