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Assessment of wheat variety distinctness using SSR markers

Journal of Integrative Agriculture 2015年第10期14卷 1923-1935页

作者： WANG li-xin QIU Jun chang li-fang liU li-hua li Hong-bo PANG Bin-shuang ZHAO chang-ping Beijing Engineering and Technique Research Center for Hybrid Wheat Beijing Academy of Agricultural and Forestry Sciences National Agriculture Technology Extension and Service Center Ministry of Agriculture National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science Chinese Academy of Agricultural Sciences

Assessment of variety distinctness is important for both the registration and the protection of particular variety. However, the current testing system, which assesses a range of morphological characters of each pair of varieties grown side-by-side, is time-consuming and is not suitable for the assessment of hundreds of samples. The objective of this study was to develop a procedure for the assessment of wheat variety distinctness using simple sequence repeat（SSR） markers. A comparison between the molecular and morphological profile of 797 varieties was made. On the basis of the comparison, pairs of varieties with a genetic similarity value（GSV） ≤90% were deemed to be distinct, accounting for ~85% of varieties assessed in wheat regional trials. For the remaining ~15% of varieties, GSVs between different varieties were 〉90%, among which ~35% were not distinct and the other ~65% were distinct. Therefore, if given a GSV〉90%, the pairs of varieties should be morphologically assessed in the field. To avoid any errors in the assessments, we proposed the elimination of contaminant plants from the sample before comparing the varietal genotypes, scoring of the genotype at each locus with a pair of allele numbers when constructing a molecular profile, and faithfully recording two alleles at a non-homozygous locus. To reduce the workload and cost, a three-grade markers comparison among varieties is suggested. In addition, 80 SSR markers and a technical procedure for assessment of wheat variety distinctness have been proposed. Based on the procedure, the distinctness assessment of ~85% of all wheat varieties is completed in our laboratory annually. Consequently, total field assessment has been reduced considerably.

关键词： wheat variety distinctness assessment SSR markers

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Genetic characteristics of a wheat founder parent and a widely planted cultivar derived from the same cross

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Journal of Integrative Agriculture 2018年第4期17卷 775-785页

作者： chang li-fang li Hui-hui WU Xiao-yang LU Yu-qing ZHANG Jin-peng YANG Xin-ming li Xiu-quan liU Wei-hua li li-hui National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization Ministry of Agriculture/Institute of Crop Sciences Chinese Academy of Agricultural Sciences

Founder parents have contributed significantly to the improvement of wheat breeding and production. In order to investigate the genetic characteristics of founder parents and widely planted cultivars, Mazhamai(M), Biyumai(B) and six sibling lines(BM1–6) derived from the cross M×B were phenotyped for eight yield-related traits over multiple years and locations and genotyped using the the wheat 90 K single nucleotide polymorphism(SNP) assay. BM4 has been used as a founder parent, and BM1 has been widely planted, whereas BM2, 3, 5, and 6 have not been used extensively for breeding or planting in China. Phenotypic comparisons revealed that BM4 and BM1 displayed a better overall performance than the other sibling lines. BM1 showed higher thousand-grain weight than BM4, whereas BM4 exhibited lower coefficient of variation for most of the yield-related traits across different years and locations, indicating that BM4 was widely adaptable and more stable in different environments. SNP analysis revealed that BM4 and BM1 inherited similar proportions of the M genome but are dissimilar to BM2, 3, 5, and 6. Both BM1 and BM4 have specific alleles that differ from the other BM lines, and most of these alleles are concentrated in specific chromosomal regions that are found to associate with favorable QTLs, these SNPs and their surrounding regions may carry the genetic determinants important for the superior performance of the two lines. But BM4 has more genetic diversity than BM1 with more specific alleles and pleiotropic regions, indicating that the genome of BM4 may be more complex than the other sibling lines and has more favorable gene resources. Our results provide valuable information that can be used to select elite parents for wheat and self-pollinating crop breeding.

关键词： wheat founder parents widely planted cultivars SNP

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Comparative studies on in vitro sperm decondensation and pronucleus formation in egg extracts between gynogenetic and bisexual fish

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Cell Research 2003年第3期13卷 159-169页

作者： chang JIAN li,JIAN fang GUIState. Key Laboratory of Freshwater Ecology and Biotechnology,Wuhan Center for Developmental Biology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China State Key Laboratory of Freshwater Ecology and Biotechnology Wuhan Center for Developmental Biology Institute of Hydrobiology Chinese Academy of Sciences Wuhan China

A cell-free system based upon the egg extracts from gynogenetic gibel carp(Carassius auratus gibelio)or bisexual red common carp(Cyprinus carpio red variety)was developed to investigate developmentalbehaviors of the demembranated sperm *** red common carp and gibel carp sperm nuclei coulddecondense fully and form pronuclei in the red common carp egg *** carp sperm nuclei couldalso decondense fully and form pronuclei in the gibel carp egg extracts,but red common carp sperum nucleicould not decondense sufficiently in the same *** significant differences of morphological changeswere further confirmed by ultrastructural observation of transmission electron *** data furtheroffer cytological evidence for gonochoristic reproduction in the gynogenetically reproducing gibel ***,the sperm nuclei in vitro decondensation is dependent on the pH in the extracts,and the decon-densed efficiency is optimal at pH ***,no DNA replication was observed in two kinds of

关键词： decondensation pronucleus formation cell-free system gynogenesis gibel carp.

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一种改进的FastICA算法

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数学的实践与认识 2019年第21期49卷 132-140页

作者：常芳丽冶继民西安电子科技大学数学与统计学院

FastICA算法是一种快速独立分量分析(Independent Component Analysis:ICA)算法,但它是基于牛顿迭代方法和合理近似的一种算法,所以具有改进空间.近年来提出了许多改进的具有更高阶收敛性质的牛顿迭代方法.将一种3阶收敛的牛顿迭代方法... 详细信息

FastICA算法是一种快速独立分量分析(Independent Component Analysis:ICA)算法,但它是基于牛顿迭代方法和合理近似的一种算法,所以具有改进空间.近年来提出了许多改进的具有更高阶收敛性质的牛顿迭代方法.将一种3阶收敛的牛顿迭代方法引入ICA算法的推导中,在合理近似的基础上,提出了一种改进的两步迭代FastICA算法.与传统FastICA算法相比,提出的改进的FastICA算法一次迭代的计算量有所增加.但是,实验结果表明,新提出的改进的FastICA算法更稳健、具有更快的收敛速度.

关键词：修正的牛顿迭代法三阶收敛性 FastICA算法盲信号分离

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Efficient and simple synthesis of B_(2)O_(3)-Al_(2)O_(3)nanocomposites assisted by deep eutectic solvent for production of 5-hydroxymethylfurfural from glucose

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Nano Research 2025年第1期18卷 236-248页

作者： Yuchen Wang Kai Rong Shanlei chang Youxing fang Shaojun Dong State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China School of Applied Chemistry and Engineering University of Science and Technology of ChinaHefei 230026China College of Chemistry Jilin UniversityChangchun 130012China

The catalytic conversion of glucose to high valueadded platform chemical 5-hydroxymethylfurfural(HMF)is a promising biorefinery process,and alumina-boria catalysts are considered to be green and mild solid acid catalysts for this catalytic ***,compared to the common synthesis methods with complicated steps,we reported a simple and efficient strategy to prepare B_(2)O_(3)-Al_(2)O_(3)nanocomposites by calcining cost-effective glucose-urea deep eutectic solvent(DES)solution containing the *** prepared B_(2)O_(3)-Al_(2)O_(3)nanocomposites exhibited an open three-dimensional skeleton and two-dimensional porous lamellar substructure,endowing them with a high specific surface area(228.27 m2/g).The introduction of boron changed the ratios of different aluminum species(AlⅣ,AlⅤ,and AlⅥ)and borate species(BO3 and BO4),thus further affecting the acidity and the types of acid sites of the *** prepared B_(2)O_(3)-Al_(2)O_(3)bifunctional acid catalysts possessing abundant Lewis acid sites and adjustable Brønsted acid sites showed complete glucose conversion and 55.38%of HMF yield under the optimum *** study proposed a concise method to synthesize alumina-boria solid acid catalysts assisted by glucose-urea *** hope to extend the application and prospect of this efficient and simple synthesis strategy.

关键词： 5-hydroxymethylfurfural(HMF) deep eutectic solvent glucose alumina-boria acid catalyst

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Interfacial Zn2+-solvation regulator towards reversible and stable Zn anode

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Journal of Energy Chemistry 2025年第1期100卷 684-692页

作者： Miao Zhou Xiongbin Luo Hang li Shan Guo Zhuang Tong Xiaotao Zhou Xu li Zhaohui Hou Shuquan liang Guozhao fang College of Mechanical Engineering Key Laboratory of Hunan Province for Advanced Carbon-based Functional Materials Hunan Institute of Science and Technology School of Materials Science and Engineering Key Laboratory of Electronic Packaging and Advanced Functional Materials of Hunan Province Central South University College of Materials and Metallurgy Guizhou University National Energy Metal Resources and New Materials Key Laboratory Central South University

Aqueous zinc-ion batteries （AZIBs） are fundamentally challenged by the instability of the electrode/electrolyte interface,predominantly due to irreversible zinc （Zn） deposition and hydrogen ***,the intricate mechanisms behind the electrochemical discrepancies induced by interfacial Zn2+-solvation and deposition behavior demand comprehensive *** molecules endowed with special functional groups （such as hydroxyl,carboxyl,etc.） have the potential to significantly optimize the solvation structure of Zn2+and regulate the interfacial electric double layer （EDL）.By increasing nucleation overpotential and decreasing interfacial free energy,these functional groups facilitate a lower critical nucleation radius,thereby forming an asymptotic nucleation model to promote uniform Zn ***,this study presents a pioneering approach by introducing trace amounts of n-butanol as solvation regulators to engineer the homogenized Zn （H-Zn） anode with a uniform and dense *** interfacial reaction and structure evolution are explored by in/ex-situ experimental techniques,indicating that the H-Zn anode exhibits dendrite-free growth,no by-products,and weak hydrogen evolution,in sharp contrast to the bare ***,the H-Zn anode achieves a remarkable Zn utilization rate of approximately 20% and simultaneously sustains a prolonged cycle life exceeding 500 ***,the H-Zn//NH4V4O10（NVO） full battery showcases exceptional cycle stability,retaining 95.04%capacity retention after 400 cycles at a large current density of 5 A *** study enlightens solvation-regulated additives to develop Zn anode with superior utilization efficiency and extended operational lifespan.

关键词： Aqueous zinc-ion batteries Interfacial reaction Asymptotic nucleation model Reversible and stable Zn anode

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小偃麦衍生系表型遗传多样性分析及综合评价

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草业学报 2022年第11期31卷 61-74页

作者：常利芳李欣郭慧娟乔麟轶张树伟陈芳畅志坚张晓军山西农业大学农学院山西太原030031

偃麦草作为小麦的三级基因库,是公认的遗传变异来源,八倍体小偃麦及其衍生的种质材料在提高小麦的遗传多样性及其抗病、品质等性状的改良上发挥了重要作用。对源于中间偃麦草和长穗偃麦草的163份小偃麦衍生系的13个农艺性状进行了多样... 详细信息

偃麦草作为小麦的三级基因库,是公认的遗传变异来源,八倍体小偃麦及其衍生的种质材料在提高小麦的遗传多样性及其抗病、品质等性状的改良上发挥了重要作用。对源于中间偃麦草和长穗偃麦草的163份小偃麦衍生系的13个农艺性状进行了多样性分析、相关分析、聚类分析、主成分分析及综合评价,结果表明:供试的小偃麦衍生系中,5个描述性质量性状的多样性指数为0.26~1.00,株型的多样性指数最高;8个产量相关性状的变异系数为4.44%~20.11%,单株产量变异最大(20.11%),其次是有效分蘖数(17.37%)和千粒重(17.13%),抽穗期最小(4.44%);多样性指数为1.97~2.09,千粒重的变异类型最为丰富(H’=2.09),其次是每穗小穗数(H’=2.07),抽穗期的多样性最低(H’=1.97);相关分析表明,不同产量性状之间相互关联;基于8个产量性状欧氏距离的系统聚类分析表明,163份衍生系被划分为6个类群,各群之间差异明显,其中第I、Ⅲ类群为产量性状优异种质;主成分分析表明,前4个主成分代表了163份衍生系81.12%的遗传信息量,分别以千粒重、每穗小穗数、穗长和株高载荷较大,对产量提高有正向效应;根据综合评价D值筛选出排名前5的优异大粒种质为CH16353、CH1742、CH16370、CH16385和CH16382。上述结果说明小偃麦衍生系存在丰富的遗传多样性,可为小偃麦种质用于小麦遗传育种提供重要的参考依据。

关键词：偃麦草小偃麦种质小麦产量性状遗传多样性综合评价

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浅析“连音”在长号演奏中的种类及应用

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辽宁高职学报 2008年第6期10卷 74-76页

作者：常黎芳沈阳音乐学院职业艺术学院辽宁沈阳110168

连音在长号演奏中具有非常重要的作用,演奏者对连音的掌握,首先要了解连音的种类,不同种类的连音对把位、气息等技巧的要求以及掌握各种技巧的练习方式,要经过严格和专业的训练来提高。通过对气息合理的运用和控制及对作品中"连音"技巧... 详细信息

连音在长号演奏中具有非常重要的作用,演奏者对连音的掌握,首先要了解连音的种类,不同种类的连音对把位、气息等技巧的要求以及掌握各种技巧的练习方式,要经过严格和专业的训练来提高。通过对气息合理的运用和控制及对作品中"连音"技巧的处理方式,使作品表现的尽善尽美,并能充分反映作品的内涵和情感。

关键词：长号演奏连音振音滑音气息音准

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网络环境下电子期刊资源的开发利用

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煤 2008年第12期17卷 81-82页

作者：常丽芳潞安矿业集团公司山西长治046204

电子期刊以网络为媒介,通过电子邮件等文件传输方式将期刊文本及时传送到用户个人计算机上,从而更好地体现了期刊内容新颖、出版迅速的特点,也为读者利用最新信息资料提供了一条快捷的通道。文章主要介绍了电子期刊的特点及利用措施。

关键词：电子期刊开发利用

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长号演奏技巧对音乐作品有效服务研究

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赤峰学院学报（哲学社会科学版） 2023年第8期44卷 45-48页

作者：常黎芳沈阳音乐学院音乐教育学院辽宁沈阳110003

长号作为西方铜管乐器的一种,以其卓越的音色越来越受到人们的喜爱和研究。众多音乐作品因为有它的参与而知名,曲作者更是因为其独特的演奏特点和不可替代的音色突出其在音乐作品中的地位,进而对演奏者的演奏技巧提出了更多、更新的要... 详细信息

长号作为西方铜管乐器的一种,以其卓越的音色越来越受到人们的喜爱和研究。众多音乐作品因为有它的参与而知名,曲作者更是因为其独特的演奏特点和不可替代的音色突出其在音乐作品中的地位,进而对演奏者的演奏技巧提出了更多、更新的要求与期望。鉴于此,本文结合典型作品中的典型乐句片段,探索长号在演奏中的呼吸控制技巧、口腔与口型配合技巧、有效吐音技巧、共鸣技巧及演奏与情感有效融合技巧等有效服务音乐作品的演奏技巧,并予以分析,总结出行其有效的演奏技巧的内容与方法。

关键词：长号音乐作品演奏技巧有效服务

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