检索结果-南通市图书馆

Comparative studies on in vitro sperm decondensation and pronucleus formation in egg extracts between gynogenetic and bisexual fish

引用

Cell Research 2003年第3期13卷 159-169页

作者： chang jian li,jian {2. {3. key Laboratory of freshwater ecology and Biotechnology,wuhan center for developmental biology,institute of hydrobiology,chinese academy of sciences,wuhan 43.072.china State key Laboratory of freshwater {2. and Biotechnology Wuhan Center for Developmental Biology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China.

A cell-free system based upon the egg extracts from gynogenetic gibel carp(Carassius auratus gibelio)or bisexual red common carp(Cyprinus carpio red variety)was developed to investigate developmentalbehaviors of the demembranated sperm *** red common carp and gibel carp sperm nuclei coulddecondense fully and form pronuclei in the red common carp egg *** carp sperm nuclei couldalso decondense fully and form pronuclei in the gibel carp egg extracts,but red common carp sperum nucleicould not decondense sufficiently in the same *** significant differences of morphological {2.eswere further confirmed by ultrastructural observation of transmission electron *** data furtheroffer cytological evidence for gonochoristic reproduction in the gynogenetically reproducing gibel ***,the sperm nuclei in vitro decondensation is dependent on the pH in the extracts,and the decon-densed efficiency is optimal at pH ***,no DNA replication was observed in two kinds of

关键词： decondensation pronucleus formation cell-free system gynogenesis gibel carp.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Screen for stage-specific expression genes between tail bud stage and heartbeat beginning stage in embryogenesis of gynogenetic silver crucian carp

引用

Cell Research 2002年第2期12卷 133-142页

作者： YAO HUA SHI, JUN liU, jian HONG XIA, jian {3. guistate.key laboratory of freshwater ecology and Biotechnology, wuhan center for developmental biology, institute of hydrobiology, chinese academy of sciences, wuhan {12., {13. 1State key laboratory of freshwater {2. and Biotechnology Wuhan Center for Developmental Biology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China 1State key laboratory of freshwater {2. and Biotechnology Wuhan Center for Developmental Biology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China 1State key laboratory of freshwater {2. and Biotechnology Wuhan Center for Developmental Biology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China 1State key laboratory of freshwater {2. and Biotechnology Wuhan Center for Developmental Biology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China

A systemic study was initiated to identify stage-specific expression genes in fish embryogenesis by using suppression subtractive hybridization (SSH) technique. In this study, we presented a preliminary result on screen for stage-specific expression genes between tail bud stage (TBS) and heartbeat beginning stage (HBS) in gynogenetic silver crucian carp (Carassius auratus gibelio). Two SSH plasmid libraries specific for TBS embryos and HBS embryos were constructed, and stage-specific expression genes were screened between the two stages. 1963.TBS positive clones and {3.66 HBS positive clones were sampled to PCR amplification, and 13.3.TBS and 1809 HBS PCR positive clones were selected to carry out dot blots. 169 TBS dot blot positive clones and {3.{3.HBS dot blot positive clones were sequenced. Searching GenBank by using these nucleotide sequences indicated that most of the TBS dot blot positive clones could not be found homologous sequences in the database, while known genes were mainly detected from HBS d

关键词： SSH embryonic development differential expression genes gynogenesis silver crucian carp.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：