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Trichoderma viride F7 improves peanut performances while remediating cadmium-contaminated soil with microplastics

Pedosphere 2024年第5期 971-980页

作者： Xin SONG Jianpeng JIN Hongliang YIN Tingjuan WANG Haiying ZONG Fangli WANG Jun LIU Xiaoli HUANG Bin WANG chao chai Zhi LI Dongsheng LIU Xuexia WANG Ningning SONG School of Resources and Environment Qingdao Agricultural University Central Laboratory Qingdao Agricultural University Institute of Soil Fertilizer and Agricultural Water Saving Xinjiang Academy of Agricultural Sciences Institute of Plant Nutrition and Resources Beijing Agricultural Forestry Academy Sciences

Microplastics(MPs) and cadmium(Cd) in soil have serious negative impacts on plant growth. As a bioremediator, Trichoderma viride may modulate the impacts of MPs and/or Cd on plant growth and heavy metal uptake. In this study, T. viride F7 was used to treat Cd-contaminated soil with and without the presence of polylactic acid MPs to investigate its effects on peanut growth, Cd uptake efficiency, and soil properties. Results showed that F7 increased soil pH,dissolved organic carbon, cation exchange capacity, microbial biomass nitrogen, and microbial biomass carbon, which resulted in increases of 2.10%–19.83% in peanut biomass and mitigated the negative effects of MPs and Cd on peanut growth. Trichoderma viride F7 reduced the soil bioavailable Cd and plant Cd concentrations by 16.68%–34.20% and 5.37%–25.84%, respectively. Regression path analysis showed that F7 could improve peanut performances by altering soil properties and reducing peanut Cd concentrations in MP-Cd-contaminated soil. Soil fungal community analysis indicated that the microbial community was altered via F7 inoculation, which antagonized Fusarium and promoted peanut growth. This study reveals the mechanisms of F7 in mitigating Cd contamination in the soil with the presence of MPs, which provides new ideas for managing MP-heavy metal-contaminated soil and improving soil health.

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MRI半自动定量磁敏感图评估终末期肾病患者脑微出血严重程度的影响因素及其与认知损害的关系

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中华放射学杂志 2024年第1期58卷 48-56页

作者：柴超刘虹岩王慧颖李金萍夏爽天津市影像医学研究所、天津市第一中心医院放射科、南开大学医学院天津300192 天津市胸科医院放射科天津300222 南开大学医学院天津300192 天津市第一中心医院肾内科南开大学医学院天津300192

目的采用半自动定量磁敏感图(QSM)探讨终末期肾病脑微出血(CMB)的总铁负荷、总体积与数量的影响因素及其与认知损害的关系。方法该研究为横断面研究。回顾性分析2018年11月至2022年8月在天津市第一中心医院就诊的具有≥1个CMB的46例终... 详细信息

目的采用半自动定量磁敏感图(QSM)探讨终末期肾病脑微出血(CMB)的总铁负荷、总体积与数量的影响因素及其与认知损害的关系。方法该研究为横断面研究。回顾性分析2018年11月至2022年8月在天津市第一中心医院就诊的具有≥1个CMB的46例终末期肾病患者的临床及影像资料。男26例、女20例,年龄42~75岁。所有患者均接受磁敏感加权成像,并通过后处理获得QSM。采用半自动的动态规划算法通过勾画CMB边界获得体积与平均磁敏感值,并计算CMB总铁负荷及总体积。采用逐步线性回归分析探索CMB数量、总体积与总铁负荷的影响因素。采用偏相关分析探索CMB与认知损害的关系,并以其他脑小血管病为协变量除外其对认知功能的影响。结果终末肾病患者CMB位于额叶19例、顶叶9例、颞叶19例、枕叶14例、基底节27例、背侧丘脑15例、半卵圆中心14例、小脑14例、脑干13例。C反应蛋白水平(95%CI 101.81~157.85,r=0.96,P=0.001)、肌酐水平(95%CI 5.32~29.61,r=0.71,P=0.010)是CMB总铁负荷的影响因素;C反应蛋白水平(95%CI 0.72~1.15,r=0.99,P=0.001)、肌酐水平(95%CI 0.03~0.22,r=0.89,P=0.014)是CMB总体积的影响因素;C反应蛋白水平(95%CI 0.10~0.12,r=0.96,P=0.001)、碱性磷酸酶水平(95%CI 0.16~0.38,r S=0.59,P=0.001)是CMB数量的影响因素。除外其他脑小血管病对认知功能的影响后,额叶CMB的总体积(r=-0.61,P=0.009)、总铁负荷(r=-0.71,P=0.002)与认知功能呈明显负相关。而额叶CMB的总数量与认知功能有负相关趋势,但不具有统计学意义(r=-0.53,P=0.063)。结论C反应蛋白水平、肌酐水平是CMB总体积、总铁负荷的影响因素;C反应蛋白水平、碱性磷酸酶水平是CMB数量的影响因素。额叶CMB总铁负荷与总体积可能为患者认知损害的生物学标记物。

关键词：磁共振成像终末期肾病脑微出血定量磁敏感图认知障碍

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库布齐沙漠柠条锦鸡儿灌木林健康评价

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西北林学院学报 2024年第1期39卷 177-184页

作者：李健左合君闫敏李进柴茵超内蒙古农业大学沙漠治理学院内蒙古呼和浩特010018 内蒙古自治区风沙物理与防沙治沙工程重点实验室内蒙古呼和浩特010018 杭锦旗林业和草原局自然保护区和天然林保护工作站内蒙古鄂尔多斯017400

随着灌木林的衰退老化,低质、低效等健康问题逐渐凸显,如何对灌木林健康状况作出评价已成为生态学领域和环境科学研究的热点之一。以库布齐沙漠柠条锦鸡儿灌木林为研究对象,通过生态学调查和等级评分方法,构建由4个一级指标,22个二级指... 详细信息

随着灌木林的衰退老化,低质、低效等健康问题逐渐凸显,如何对灌木林健康状况作出评价已成为生态学领域和环境科学研究的热点之一。以库布齐沙漠柠条锦鸡儿灌木林为研究对象,通过生态学调查和等级评分方法,构建由4个一级指标,22个二级指标组成的柠条锦鸡儿健康评价指标体系,具有便捷性和适用性。采用专家打分法和AHP层次分析法确定各指标权重,并利用生态系统健康综合指数模型计算。结果表明,库布齐沙漠柠条锦鸡儿灌木林整体综合健康指数为0.5387,健康、轻度低质低效、中度低质低效、重度低质低效占比分别为7.69%、38.46%、46.16%与7.69%,整体处于中度低质低效健康状态,有向重度低质低效发展的趋势。林分活力、土壤条件、群落结构和枯落物条件等是影响柠条锦鸡儿灌木林健康主要因素。

关键词：柠条锦鸡儿健康评价层次分析法库布齐沙漠

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Effects of nitrogen and phosphorus concentrations on the bioaccumulation of polybrominated diphenyl ethers by Prorocentrum donghaiense

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Journal of Environmental Sciences 2013年第2期25卷 376-385页

作者： chao chai Xundong Yin Wei Ge Jinye Wang College of Resources and Environment Qingdao Agricultural University College of Life Sciences Qingdao Agricultural University College of Animal Science and Veterinary Qingdao Agricultural University

The growth, cellular total lipids, bioaccumulation amount, and bioaccumulation factors （BAFs） of 2,4,4 ′ -tribromodiphenyl ether （BDE28）, 2,2 ′ ,4,4 ′ -tetrabromodiphenyl ether （BDE47）, and 2,2 ′ ,4,4 ′ ,5-pentabromodiphenyl ether （BDE99） in a semi-continuous culture of Prorocentrum donghaiense were studied in relation to nitrate （0, 128, and 512 μmol/L） and phosphate （0, 8, and 32 μmol/L） concentrations. The BDE28, BDE47, and BDE99 content per cell under 0 μmol N/L were 3.77 × 10 6 , 3.95 × 10 6 , and 4.32 × 10 6 ng/cell, respectively, which were significantly higher than those under 128 and 512 μmol N/L. A nearly 5-fold increase in polybrominated diphenyl ether （PBDE） content per algal cell was found between 0 and 8 μmol P/L and between 8 and 32 μmol P/L. With increasing N and P concentrations, the PBDE content per volume of algal culture and the accumulation percentage of available PBDEs declined slightly. The BAFs for the PBDEs based on lipids showed that the logBAF lip under 0 μmol N/L was higher than those under 128 and 512 μmol N/L. The logBAF lip under 0 μmol P/L was higher than that under 8 μmol P/L but lower than that under 32 μmol P/L. Correlation analysis indicated a significant negative correlation between nutrient concentration and cellular total lipids, as well as the PBDE content per cell. The results indicate that different N and P concentrations change the total lipids content of P. donghaiense, thereby resulting in varying PBDE accumulation.

关键词： nitrogen phosphorus bioaccumulation polybrominated diphenyl ethers Prorocentrum donghaiense

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Variation of Bioaccumulation Ability of 2,2',4,4'-Tetrabromodiphenyl Ether by Marine Diatom Skeletonema costatum Under Different N:P Ratios

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Journal of Ocean University of China 2014年第3期13卷 523-530页

作者： chai chao GE Wei YIN Xundong College of Resources and Environment Qingdao Agricultural University College of Life Sciences Qingdao Agricultural University

The growth, biochemical content and bioaccumulation quantity of 2,2',4,4'-tetrabromodiphenyl ether(BDE-47) in Skeletonema costatum were studied under different N:P ratios(1, 4, 16, 64 and 128). All cellular biochemical contents of S. costatum presented decreasing trend over cultivation time. At early stage of cultivation, the cellular protein, carbohydrate and lipid in S. costatum presented higher values in treatments of N:P=4 and 16. However, they were lower in these treatments at the late stage, but higher in treatments N:P=1 and 128. Similarly, BDE-47 levels per cell of S. costatum were higher in treatments of N:P=4 and 16 at early stage of cultivation, which were 3.8 and 3.7 ng(106 cells)-1, respectively. At the middle stage of cultivation, the BDE-47 level per S. costatum cell lowered; and it further reduced in the treatments of N:P=4 and 16 at the late stage with the values 0.6 and 0.5 ng(106 cells)-1, respectively. However, it rose in N:P=128, reaching up to 2.3 ng(106 cells)-1. Compared with BDE-47 per cell, BDE-47 per algal volume under different N:P ratios did not present obvious difference. The quantity BDE-47 accumulated per cell of S. costatum was positively correlated with protein, carbohydrate and lipid per cell; meanwhile, the BDE-47 per volume had a positive correlation with biochemical content per volume. The variation of bioaccumulation ability of BDE-47 in S. costatum can be explained by biochemical changes due to N:P ratios.

关键词： N:P ratio bioaccumulation polybrominated diphenyl ethers microalgae nutrient

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基于BIM与驾驶模拟的可变信息标志布设间距研究

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公路 2024年第7期69卷 172-177页

作者：柴振超杨格肖忆焜邓敏张奥宇广东湾区交通建设投资有限公司广州市510699 中交第二公路勘察设计研究院有限公司武汉市430056

为确定高等级立体复合通道可变信息标志的安全布设间距,基于狮子洋通道设计方案开展了驾驶模拟试验。试验选取了相邻路段车速差值、运行车速与设计车速差值、车速降低系数为分析指标,共采集30名驾驶员的试验数据。结果显示,不同布设间... 详细信息

为确定高等级立体复合通道可变信息标志的安全布设间距,基于狮子洋通道设计方案开展了驾驶模拟试验。试验选取了相邻路段车速差值、运行车速与设计车速差值、车速降低系数为分析指标,共采集30名驾驶员的试验数据。结果显示,不同布设间距会影响驾驶员对行车速度的决策,从而对行车安全性产生影响,其中2 km间距方案对速度影响相对平顺,安全性较高。

关键词：高速公路可变信息标志 BIM 驾驶模拟布设间距安全性评价

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Molecular characterization and functional analysis of a piscidin gene in large yellow croaker (Larimichthys crocea)

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Zoological Research 2016年第6期37卷 347-355页

作者： Jing YANG Xin-Jiang LU Fang-chao chai Jiong CHEN Laboratory of Biochemistry and Molecular Biology School of Marine SciencesNingbo University Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture Ningbo University

The piscidin family, which includes potent antimicrobial peptides with broad-spectrum activity, plays an important role in the innate immune system of fish. In this study, we cloned piscidin-5-1ike type 3 （Lcpis51t3） in large yellow croaker （Larimichthys crocea）. Multiple alignments with other known piscidins revealed amino acid conservation throughout the fish, especially at the signal peptide （22 amino acids）. The phylogenetic tree confirmed that Lcpis51t3 and large yellow croaker piscidin-5-1ike proteins were grouped together to form a branch. Quantitative real-time PCR revealed that Lcpis51t3 was expressed in a wide range of tissues, including the brain, muscle, gill, head kidney, intestine, kidney, liver, and spleen. The highest mRNA expression level of Lcpis51t3 was found in the spleen. After Vibrio alginolyticus infection, mRNA expression was rapidly upregulated in the liver, head kidney, gill, kidney, and intestine at 4, 8, 12, and 24 h post infection （hpi）, whereas there were no significant changes in the spleen. The antimicrobial spectrum showed that the synthetic mature peptide of Lcpis51t3 exhibited different activity in vitro against various bacteria, such as Aeromonas hydrophila, V. anguillarum, V. alginolyticus, V. parahaemolyticus, Staphylococcus aureus, and Listeria monocytogenes In addition, survival rates from the in vivo assay indicated that the synthetic peptide of Lcpis51t3 increased the survival rate of large yellow croaker after V. alginolyticus challenge, resulting in a decline in bacterial burden and mRNA expression levels of interleukin-ll3, interleukin-10, and tumor necrosis factor-（]. These data suggest that Lcpis51t3 plays an important role in innate immunity in large yellow croaker and might represent a potential therapeutic agent against pathogen invasion.

关键词： Antimicrobial activity Large yellowcroaker Piscidin Survival rate Vibrio alginolyticus

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Ultrasmall Nanoparticles Diluted chain Entanglement in Polymer Nanocomposites

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Chinese Journal of Polymer Science 2019年第8期37卷 797-805页

作者： Sheng-chao chai Tian-Yang Xu Xiao Cao Gang Wang Quan Chen Hao-Long Li State Key Laboratory of Supramolecular Structure and Materials College of Chemistry Jilin University Changchun 130012 China State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China

Nanoparticle-polymer composites exhibit unusual mechanical properties and chain dynamics when the nanoparticle size is smaller than the entanglement mesh size of the matrix polymer chains, corresponding to the ultrasmall regime defined by de ***, the mechanism is still ambiguous due to the lack of suitable model systems. Here, we develop an ultrasmall nanoparticle system by using a bimodal grafting strategy to graft both short alkyl chains and long polystyrene chains onto the polyoxometalate molecular nanoparticles with a tunable repulsive potential between the nanoparticles, thus facilitating their uniform dispersion in polystyrene matrices. Linear viscoelasticity of the resultant nanocomposites changes with increasing the filler content, which shows a decrease in both plateau modulus and terminal relaxation time, indicative of a dilution effect of the nanoparticles. Namely, the entanglement network becomes sparser with increasing the filler content.

关键词： Polymer nanocomposites Ultrasmall nanoparticles chain entanglement Polyoxometalates

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Temporal and Spatial Variations of Abundance of Phycocyaninand Phycoerythrin-Rich Synechococcus in Pearl River Estuary and Adjacent Coastal Area

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Journal of Ocean University of China 2016年第5期15卷 897-904页

作者： JIANG Tao chai chao WANG Jifang ZHANG Ling CEN Jingyi LU Songhui Key Laboratory of Sustainable Development of Marine Fisheries of Ministry of Agriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao Engineering Research Center for Rural Environment Qingdao Agricultural University College of Life Science and Technology Jinan University

Three surveys were carried out in Pearl River Estuary and adjacent coastal area in May, August, and November, 2013, to investigate the temporal and spatial variations of abundance of phycoerythrin-rich Synechococcus(PE-rich SYN) and phycocyanin-rich Synechococcus(PC-rich SYN). The effects of environmental factors on the alternation of the different Synechococcus groups were also elucidated. PE-rich SYN was detected in three surveys, whereas PC-rich SYN was detected in May and August, but not in November. The highest abundances of PE-rich SYN and PC-rich SYN were recorded in August and May, with mean values of 74.17×103 and 189.92×103 cells m L-1, respectively. From May to November, the relative abundance of PE-rich SYN increased, whereas that of PC-rich SYN declined. PE-rich and PC-rich SYN presented similar horizontal distributions with high abundance in the southern estuary in May, and in the western estuary in August. The abundances of PE-rich and PC-rich SYN were high at 27–32℃and salinity of 10–20. PC-rich SYN was not detected at < 24℃, and PC:PE-rich SYN decreased in abundance with salinity increase. When less than 20 mg L-1, suspended particulate matter(SPM) was helpful for Synechococcus growth. PE-rich SYN decreased in abundance when the concentration of dissolved inorganic nitrogen increased in May and November, and the concentration of phosphate increased in November. However, PC-rich SYN abundance and nutrients showed no correlation. Principal component analysis and regression analysis indicated that PE-rich SYN significantly correlated with the principal components that were affected by environmental factors.

关键词： Pearl nutrients suspended declined dissolved abundance ammonium inorganic estuary salinity

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Piezoelectric Energy Analysis on Diverse Buoy Coupling with Hydrodynamic Parameters

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China Ocean Engineering 2019年第3期33卷 279-287页

作者： LIU Heng-xu LIU Ming chai Yuan-chao SHU Guo-yang JING Feng-mei WANG Li-quan College of Shipbuilding Engineering Harbin Engineering University Harbin 150001 China College of Mechanical and Electrical Engineering Harbin Engineering University Harbin 150001 China

This paper mainly describes the influence factors of the captured energy power by huge wave energy harvesters, in which the vertical motion of buoy can transform ocean’s potential energy into piezoelectric energy power by undulating waves. Firstly, related environmental coefficients are analyzed by means of the incident wave theory. Besides, the geometric structural parameters are also analyzed and compared under optimal environmental coefficients with semi-analytical solutions. Thirdly, the numerical results also show the impact trend of hydrodynamic parameters and geometric volume on motion, voltage and power with qualitative agreement. The numerical simulation confirms that the improved structure parameters could markedly deliver sufficient power under the same conditions with long-time stability.

关键词： semi-analytical method incident wave theory piezoelectric cantilever beam hydrodynamic characteristics lumped and structural parameters

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