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Neuroprotective potential for mitigating ischemia-reperfusion-induced damage

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Neural Regeneration Research 2025年第8期20卷 2199-2217页

作者： Zi Ye Runqing Liu Hangxing Wang Aizhen Zuo cen Jin Nan Wang Huiqi Sun Luqian Feng Hua Yang The Clinical Medical College Guizhou Medical UniversityGuiyangGuizhou ProvinceChina Division of Infectious Diseases Department of Internal MedicinePeking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina School of Medical Imaging Guizhou Medical UniversityGuiyangGuizhou ProvinceChina Division of Gastroenterology First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenan ProvinceChina The Second Clinical Medical College Lanzhou UniversityLanzhouGansu ProvinceChina Department of Neurosurgery Affiliated Hospital of Guizhou Medical UniversityGuiyangGuizhou ProvinceChina

Reperfusion following cerebral ischemia causes both structural and functional damage to brain tissue and could aggravate a patient's condition;this phenomenon is known as cerebral ischemia-reperfusion *** studies have elucidated the neuroprotective role of the sirtuin protein family(Sirtuins)in modulating cerebral ischemia-reperfusion ***,the potential of utilizing it as a novel intervention target to influence the prognosis of cerebral ischemia-reperfusion injury requires additional *** this review,the origin and research progress of Sirtuins are summarized,suggesting the involvement of Sirtuins in diverse mechanisms that affect cerebral ischemia-reperfusion injury,including inflammation,oxidative stress,blood-brain barrier damage,apoptosis,pyroptosis,and *** therapeutic avenues related to Sirtuins that may improve the prognosis of cerebral ischemia-reperfusion injury were also investigated by modulating Sirtuins expression and affecting representative pathways,such as nuclear factor-kappa B signaling,oxidative stress mediated by adenosine monophosphate-activated protein kinase,and the forkhead box *** review also summarizes the potential of endogenous substances,such as RNA and hormones,drugs,dietary supplements,and emerging therapies that regulate Sirtuins *** review also reveals that regulating Sirtuins mitigates cerebral ischemia-reperfusion injury when combined with other risk *** Sirtuins show promise as a potential target for the treatment of cerebral ischemiareperfusion injury,most recent studies are based on rodent models with circadian rhythms that are distinct from those of humans,potentially influencing the efficacy of Sirtuinstargeting drug ***,this review provides new insights into the role of Sirtuins in the pathology and treatment of cerebral ischemia-reperfusion injury.

关键词： apoptosis autophagy blood-brain barrier dietary supplements drug hormones inflammation neuroprotection oxidative stress prognosis pyroptosis reperfusion injury risk factors RNA therapeutics

过量表达自身hemA基因的重组大肠杆菌发酵生产5-氨基乙酰丙酸的研究

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生物加工过程 2005年第4期3卷 36-39,53页

作者：赵春晖林建平刘晓侠穆江华岑沛霖浙江大学化学工程与生物工程学系生物工程研究所杭州310027

研究了优化重组大肠杆菌产5-氨基乙酰丙酸(ALA)的条件,提高大肠杆菌发酵生产ALA的产量。在测定重组大肠杆菌GT48的生长曲线的基础上,确定诱导时间,优化摇瓶发酵条件。然后,进一步在5 L发酵罐上进行间歇和流加发酵研究。摇瓶实验表明,细... 详细信息

研究了优化重组大肠杆菌产5-氨基乙酰丙酸(ALA)的条件,提高大肠杆菌发酵生产ALA的产量。在测定重组大肠杆菌GT48的生长曲线的基础上,确定诱导时间,优化摇瓶发酵条件。然后,进一步在5 L发酵罐上进行间歇和流加发酵研究。摇瓶实验表明,细胞培养最佳初始pH为6.5,最佳诱导时间为稳定期前期,最佳接种量为2%,过高的葡萄糖浓度对细胞生长和产物合成均有一定的抑制作用。在5 L发酵罐间歇发酵中,重组菌产ALA能力达到47.8 mg/L。采用流加发酵可以进一步将产物产量提高到63.8 mg/L。构建的过量表达自身的hemA基因的大肠杆菌具有较高的产ALA能力,通过发酵条件优化和采用流加发酵可以提高ALA产量。

关键词： 5-氨基乙酰丙酸重组大肠杆菌发酵流加操作

化妆品用人表皮生长因子的离子交换法纯化和脱色工艺研究

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浙江工业大学学报 2005年第6期33卷 626-629页

作者：赵肖为徐志南周茂洪岑沛霖温州大学应用技术学院浙江温州325035 浙江大学生物工程研究所浙江杭州310027

开发一套应用离子交换技术、以生产满足化妆品使用要求为目的的人表皮生长因子纯化和脱色工艺,脱色用离子交换树脂为D314.纯化条件:流速为150.0 mL/h,进样体积为80.0 mL,床高为10.0 cm;脱色条件:流速为60.0 mL/h,pH=5.0和床高为7.5 cm.... 详细信息

开发一套应用离子交换技术、以生产满足化妆品使用要求为目的的人表皮生长因子纯化和脱色工艺,脱色用离子交换树脂为D314.纯化条件:流速为150.0 mL/h,进样体积为80.0 mL,床高为10.0 cm;脱色条件:流速为60.0 mL/h,pH=5.0和床高为7.5 cm.总收率超过53%,产品纯度高于32%,色泽呈白色或浅黄色,能够满足化妆品行业的使用要求.

关键词：人表皮生长因子离子交换纯化脱色

重组人粒细胞刺激因子在《中国药典》、《美国药典》和《欧洲药典》中的质量标准对比分析

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齐鲁药事 2011年第2期30卷 76-79页

作者：徐小蓉郭旺明黄磊蔡谨徐志南岑沛霖浙江华阳药业有限公司浙江杭州310053 浙江大学化工系生物工程研究所浙江杭州310027 杭州九源基因工程有限公司浙江杭州310018

目的对比重组人粒细胞刺激因子(rhG-CSF,Filgrastim)在《中国药典》(2010年版)、《美国药典》33版初稿和《欧洲药典》(7.0版)中标准的差异,为国内研发机构在rhG-CSF质量标准的提高研究提供参考。方法按三部药典要求,对rhG-CSF产品进行... 详细信息

目的对比重组人粒细胞刺激因子(rhG-CSF,Filgrastim)在《中国药典》(2010年版)、《美国药典》33版初稿和《欧洲药典》(7.0版)中标准的差异,为国内研发机构在rhG-CSF质量标准的提高研究提供参考。方法按三部药典要求,对rhG-CSF产品进行主要关键指标的检测分析。结果《中国药典》在对该产品的标准描述上不如《欧洲药典》(7.0版)和《美国药典》33版初稿,而《美国药典》33版初稿的rhG-CSF标准要求最高。三部药典存在具体内容的区别。结论提高rhG-CSF现行产品质量标准可参照《美国药典》33版初稿。

关键词：重组人粒细胞刺激因子美国药典欧洲药典中国药典

Vif基因体外表达载体构建及其在无细胞系统中的表达

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科技通报 2007年第3期23卷 356-359,367页

作者：汪诚陈海琴徐志南殷秀飞岑沛霖浙江大学生物工程研究所杭州310027 浙江养生堂天然药物研究所杭州310007

随着-omics(组学)时代的到来,无细胞蛋白质合成系统以具有快速、方便、易于高通量等优点,正在被广泛地研究和应用。本文选取HIV病毒感染因子Vif作为目标蛋白,构建了适宜体外表达的Vif表达载体pIVEX2.4c-Vif,并将其在大肠杆菌无细胞蛋白... 详细信息

随着-omics(组学)时代的到来,无细胞蛋白质合成系统以具有快速、方便、易于高通量等优点,正在被广泛地研究和应用。本文选取HIV病毒感染因子Vif作为目标蛋白,构建了适宜体外表达的Vif表达载体pIVEX2.4c-Vif,并将其在大肠杆菌无细胞蛋白质合成系统中进行表达,为下一步进行高通量药物筛选奠定了一定的基础。

关键词： HIV 生物合成无细胞合成系统 Vif(virion infectivity factor)

Characteristics and Weldability of Resistance Plug Welding of Dissimilar Steels

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Journal of Wuhan University of Technology(Materials Science) 2023年第6期38卷 1463-1471页

作者： cen Yaodong CHEN lin MA Xiao School of Materials and Metallurgy Inner Mongolia University of Science and TechnologyBaotou 014010China

TRIP980 high-strength steel plate/SPCC low-carbon steel plate were welded by RPW. The key factors such as size and material of filler were studied, and the structure, fusion ratio and mechanical properties of the RPW joint were analyzed. The experimental results show that the calculation formulas of the length and diameter of the filler were designed reasonably. Q235 as a filler for RPW of TRIP980 high-strength steel plate/SPCC low-carbon steel plate is suitable according to schaeffler organization chart. The deposited metal of RPW joint is in the shape of “spool”,and the base metal and cap of deposited metal are alternately combined. The deposited metal has the characteristics of “locking” as rivets, which is beneficial to the improvement of mechanical properties of RPW joint. The nugget of RPW joint is uniform without deviates. TRIP980 high-strength steel plate, SPCC low-carbon steel plate, and filler were metallurgically bonded in the RPW joint.

关键词： dissimilar steel resistance plug welding nugget

A Lightweight Compact Lithium-Ion Battery Thermal Management System Integratable Directly with EV Air Conditioning Systems

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Journal of Thermal Science 2022年第6期31卷 2363-2373页

作者： lin Bichao cen Jiwen JIANG Fangming Laboratory of Advanced Energy Systems Guangzhou Institute of Energy ConversionChinese Academy of SciencesGuangzhou510640China CAS Key Laboratory of Renewable Energy Guangzhou 510640China Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development Guangzhou 510640China University of Chinese Academy of Sciences Beijing 100049China

This paper discusses an improved thermal management system to ameliorate the performance of lithium-ion battery storage systems for electric vehicles(EVs) applications. A compact and lightweight cold plate is designed and fabricated to fit 18650-type lithium-ion batteries, using aluminum-finned copper tubes. A dynamic temperature PID(proportional, integral, differential) control algorithm for electronic expansion valves is developed to study using EV air conditioning refrigerant, R134a, to control battery modules’ temperature with this compact and lightweight thermal management system. The experimental results show that the proposed battery thermal management system can effectively control the battery module’s temperature. In addition, during 1C discharge, when the PID temperature algorithm control scheme is used, the maximum temperature difference across the battery module peaks at less than 4℃, and the maximum temperature within the battery module is less than 36℃.

关键词： electric vehicles air conditioning system battery thermal management finned tube two-phase flow

A novel radial cable for restraining tensile failure in steep fill–rock interfaces

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Journal of Mountain Science 2019年第7期16卷 1715-1730页

作者： HUANG Da LI pei-ji cen Duo-feng School of Civil and Transportation Engineering Hebei University of Technology

It is quite common for fill slopes such as embankments and airport foundations to be constructed in mountainous *** fill–rock interfaces in such areas undergo tensile failure owing to differences in the stiffness values and tensile strengths of the soil and hard *** this study,a new anchor system,termed“radial cable,”is proposed to increase the pullout capacity of cables in the fill;this in turn helps control tensile deformation in the fill slope along the *** a radial cable,the steel ropes of a conventional cable are equally and symmetrically separated into three sub-cables(a central sub-cable and two branch sub-cables)with a radial distribution starting from the position of the ***,each of the sub-cables is fixed in the fill using short U-shaped rigid rods along the cable length and a rigid baffle at its end to further increase pullout *** and numerical uplift tests were conducted to analyze the pullout capacity and anchoring behavior of the radial cables in soil to study the effect of *** reinforcement effect of the radial cables was also examined using a case study.

关键词： Fill slope Fill–rock interface Tensile failure Radial cable Pullout capacity

毛细管电泳用于1,4-丁二磺酸二钠盐分析及其制备过程的研究

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科技通报 2006年第6期22卷 737-741页

作者：赵晓星徐志南沈文和孔领伟石峰岑沛霖浙江大学化工系生物工程所杭州310027 浙江埃森医药有限公司中央研究所生物工程中心浙江横店322118

研究了合成1,4-丁二磺酸二钠盐的工艺。在1,4-丁二磺酸二钠盐的合成过程中,由于在反应体系中加入表面活性剂EDTA,最佳反应时间只要约3 h,与文献[1]中长达36 h相比,大大缩短了反应时间,反应平均转化率为99.39%。建立了用高效毛细管电泳(H... 详细信息

研究了合成1,4-丁二磺酸二钠盐的工艺。在1,4-丁二磺酸二钠盐的合成过程中,由于在反应体系中加入表面活性剂EDTA,最佳反应时间只要约3 h,与文献[1]中长达36 h相比,大大缩短了反应时间,反应平均转化率为99.39%。建立了用高效毛细管电泳(HPCE)测定1,4-丁二磺酸的定量分析方法。分析条件为:非涂层毛细管柱为50μm×81 cm(AGILENT),缓冲液:20 mmol硼砂溶液(pH=9.24),检测波长:191.2 nm,电压进样,进样电压30 kV,进样时间5 s。对标准曲线、精密度、准确度及重现性进行了研究。该方法的线性相关系数0.999,日内变异系数0.8%,日间变异系数0.5%,回收率96.5%。HPCE法具有准确性高、重现性好、灵敏度高及快速测定的优点,非常适合于1,4-丁二磺酸二钠盐含量的测定。

关键词： 1,4-丁二磺酸二钠盐 1,4-丁二磺酸合成分析 HPCE EDTA