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Liquid crystal immunosensors for the selective detection of Escherichia coli with a fast analysis tool

Photonics Research 2024年第7期12卷 1564-1573页

作者： SANDRO C.OLIVEIRA MARIA S.SOARES BÁRBARA V.GONÇALVES ANDREIA C.M.RODRIGUES AMADEU M.V.M.SOARES RITA G.SOBRAL NUNO F.SANTOS JAN NEDOMA PEDRO L.ALMEIDA carlos marques CICECO-Aveiro Institute of Materials Physics DepartmentUniversity of Aveiro3810-193 AveiroPortugal i3N Physics DepartmentUniversity of Aveiro3810-193 AveiroPortugal Associate Laboratory i4HB-Institute for Health and Bioeconomy School of Sciences and TechnologyNOVA University of Lisbon1099-085 LisbonPortugal CESAM-Centre for Environmental and Marine Studies and Department of Biology University of Aveiro3810-193 AveiroPortugal Department of Telecommunications VSB-Technical University of OstravaOstrava 70800Czech Republic i3N-CENIMAT School of Sciences and TechnologyNOVA University of Lisbon1099-085 LisbonPortugal UnIRE ISELPolytechnic Institute of Lisbon1549-020 LisbonPortugal Department of Physics VSB-Technical University of OstravaOstrava 70800Czech Republic

The consumption of contaminated food may cause serious illnesses,and traditional methods to detect Escherichia coli are still associated with long waiting times and high costs given the necessity to transport samples to specialized *** is a need to develop new technologies that allow cheap,fast,and direct monitoring at the site of ***,in this work,we developed optical immunosensors for the selective detection of ***,based on liquid crystal technology,whose molecules can align in different manners depending on the boundary conditions (such as substrates) as well as the environment that they *** glass substrate was functionalized with *** antibody using cysteamine as an intermediate,and a vertical alignment was imposed on the liquid crystal molecules by using DMOAP during *** presence of bacteria disrupts the alignment of the liquid crystal molecules,changing the intensity of light emerging between cross polarizers,measured using a polarized optical microscope and a *** was possible to detect *** in suspensions in the concentration range from 2.8 cells/mL to 2.8×10^(9) cells∕*** was also evaluated,and the sensors were used to analyze contaminated water samples.A prototype was developed to allow faster,in-situ,and easier analysis avoiding bulky instruments.

关键词： crystal alignment analysis

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Carbon Stock Assessment at a Regional Level, on Pure and Mixed Stands, Based on Inventory Data: A Case Study

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Open Journal of Forestry 2011年第1期1卷 6-9页

作者： Teresa Fidalgo Fonseca carlos Pacheco marques Department of Forest Science and Landscape Architecture University of Tras-os-Montes e Alto Douro

Following Kyoto protocol considerable research efforts have been made on carbon sequestration assessment of forest stands. The aim of the present study was to test the adequacy of two documented methodologies to obtain stand level dry biomass and carbon estimates, considering its application at regional scale, for pure and mixed stands. The procedures refer to: 1) the estimation of dry biomass through the use of tree biomass models and 2) the use of biomass conversion and expansion factors (BCEF) to convert stem volume raw data into aboveground dry biomass. Using the dry biomass values, carbon stock is easily estimated using conversion factors, being 0.50 a generally accepted value. Case study reports to a pilot zone with pure and mixed stands of Eucalyptus globulus Labill and Pinus pinaster Ait. in Portugal. Procedure 1) gives the most reliable results when the database includes detailed information at tree level, both for pure or mixed stands. When individual tree data is not accessible, option 2) is an interesting option to consider for pure stands. The use of BCEF-based procedure in mixed stands is not advocated at least when considering the direct use of a single conversion factor, as the value of the factor strongly depends on the forest composition.

关键词： Biomass BCEF Greenhouse Inventory Carbon Monitoring

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Photonic smart bandage for wound healing assessment

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Photonics Research 2021年第3期9卷 272-280页

作者： Arnaldo Leal-Junior Jingjing Guo Rui Min Antonio Jose Fernandes Anselmo Frizera carlos marques Telecommunications Laboratory(LABTEL) Graduate Program in Electrical EngineeringFederal University of Espi’rito SantoVitoria-ES 29075-910Brazil School of Instrumentation and Optoelectronic Engineering Beihang UniversityBeijing 100191China Center for Cognition and Neuroergonomics State Key Laboratory of Cognitive Neuroscience and LearningBeijing Normal UniversityZhuhai 519087China Physics Department&I3N Universidade de AveiroCampus Universitario de SantiagoAveiro 3810-193Portugal

Chronic wounds affect around 2% of the world population with an annual multi-billion dollar cost to the healthcare system. This background pushes the development of new therapies and procedures for wound healing and its assessment. Among them, the potential of hydrogen(pH) assessment is an important indicator of the wound healing stage and condition. This paper presents the development of the first optical fiber-embedded smart wound dressing for pH assessment. An intrinsically pH-sensitive optical fiber is fabricated using a polydimethylsiloxane(PDMS) precursor doped with rhodamine B dye. Raman and Fourier transform infrared(FTIR) spectroscopies are performed in order to verify the presence of rhodamine B and PDMS in the fiber samples. Then, the fiber is embedded in gauze fabric and hydrocolloid wound dressing. In addition, such low Young’s modulus of PDMS fiber enables its use as a highly sensitive pressure sensor, where the results show that the fiber-embedded bandage can measure pressures as low as 0.1 kPa with a high linearity in the range of 0 to 0.3 kPa. The smart bandage is subjected to different pH, which resulted in a wavelength shift of 0.67 nm/pH when the absorption peak at 515 nm was analyzed. Furthermore, pH increase leads to linear decrease of the transmitted optical power(R2 of 0.998), with rise and fall times below 20 s and 30 s, respectively. Therefore, the proposed optical fiber-embedded smart bandage enables the simultaneous assessment of pressure and pH on the wound region.

关键词： fiber smart healing

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Botanical Aspects,Caffeine Content and Antioxidant Activity of Coffea arabica

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American Journal of Plant Sciences 2019年第6期10卷 1013-1021页

作者： Andreia marques dos Santos Luis carlos marques Carolina Passarelli Goncalves Maria Cristina Marcucci Post-Graduation Program in Pharmacy and Biotechnology of the Anhanguera University of Sao Paulo OsascoBrazil Pharmacy Department of Anhanguera University of Sao Paulo OsascoBrazil

Brazil is the largest coffee exporter in the world. In addition, it occupies the second position, among the consuming countries of the drink. To investigate the chemical composition and quality of the coffee drink from Coffea arabica species, samples grown in the city of Ourinhos, the third most productive region in the state of Sao Paulo, a study of its properties and characteristics was conducted. The pharmacobotanical characteristics were investigated performed according to usual techniques in these researches through macroscopic and microscopic studies through cross-sections. The oil obtained for analysis was extracted by soxhlet, and the caffeine content was measured for green grains using High Performance Liquid Chromatography (HPLC) using as stationary phase, column C18 and gradient mobile phase formed by 80% water and 20% methanol. The concentration obtained was 44.983 ± 0.86 μg/mL. The antioxidant activity was measured in triplicate through the DPPH test of the in natura coffee oil, and presented antioxidant action of EC50 25.89 ± 1.16 μg/mL.

关键词： Coffea arabica Botanical Characterization Caffeine

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Sirtuin 1 in rat orthotopic liver transplantation:An I GL-1 preservation solution approach

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World Journal of Gastroenterology 2015年第6期21卷 1765-1774页

作者： Eirini Pantazi Mohamed Amine Zaouali Mohamed Bejaoui Emma Folch-Puy Hassen Ben Abdennebi Ana Teresa Varela Anabela Pinto Rolo carlos marques Palmeira Joan Roselló-Catafau Experimental Hepatic Ischemia Reperfusion Unit Institute of Biomedical Research of Barcelona Faculty of Pharmacy Molecular Biology and Anthropology Applied to Development and Health Department of Life Sciences and Center for Neuroscience and Cell Biology University of Coimbra Department of Biology University of Aveiro

AIM:To investigate the possible involvement of Sirtuin1(SIRT1)in rat orthotopic liver transplantation(OLT),when Institute Georges Lopez 1(IGL-1)preservation solution is enriched with trimetazidine(TMZ).METHODS:Male Sprague-Dawley rats were used as donors and *** were stored in IGL-1 preservation solution for 8h at 4℃,and then underwent OLT according to Kamada’s cuff technique without *** another group,livers were stored in IGL-1 preservation solution supplemented with TMZ,at10-6 mol/L,for 8 h at 4℃and then underwent *** were sacrificed 24 h after reperfusion,and liver and plasma samples were *** injury(transaminase levels),mitochondrial damage(glutamate dehydrogenase activity)oxidative stress(malondialdehyde levels),and nicotinamide adenine dinucleotide(NAD+),the cofactor necessary for SIRT1 activity,were determined by biochemical ***1 and its substrates(acFox O1,ac-p53),the precursor of NAD+,nicotinamide phosphoribosyltransferase(NAMPT),as well as the phosphorylation of adenosine monophosphate activated protein kinase(AMPK),p-m TOR,p-p70S6K(direct substrate of m TOR),autophagy parameters(beclin-1,LC3B)and MAP kinases(p-p38 and p-ERK)were determined by Western ***:Liver grafts preserved in IGL-1 solution enriched with TMZ presented reduced liver injury and mitochondrial damage compared with those preservedin IGL-1 solution *** addition,livers preserved in IGL-1+TMZ presented reduced levels of oxidative *** was consistent with enhanced SIRT1 protein expression and elevated SIRT1 activity,as indicated by decreased acetylation of p53 and Fox *** elevated SIRT1 activity in presence of TMZ can be attributed to the enhanced NAMPT protein and NAD+/NADH ***-regulation of SIRT1 was consistent with activation of AMPK and inhibition of phosphorylation of m TOR and its direct substrate(p-p70S6K).As a consequence,autophagy mediators(beclin-1 and LC3B)were ***,MAP kinases w

关键词： Sirtuin 1 Ischemia-reperfusion injury Liver transp

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Machine learning-based analysis of multiple simultaneous disturbances applied on a transmission-reflection analysis based distributed sensor using a nanoparticle-doped fiber

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Photonics Research 2023年第3期11卷 364-372页

作者： LETíCIA AVELLAR ANSELMO FRIZERA HELDER ROCHA MARIANA SILVEIRA CAMILO DíAZ WILFRIED BLANC carlos marques ARNALDO LEAL-JUNIOR Graduate Program of Electrical Engineering Federal University of Espirito SantoVitoria 29075-910Brazil Université Cote d'Azur Institut de Physique de NiceCNRS06108 Nice Cedex 2France I3N and Physics Department Universidade de AveiroCampus Universitário de SantiagoAveiro 3810-193Portugal

Photonic technology combined with artificial intelligence plays a key role in the development of the latest smart system trends,integrating cutting-edge technology with machine learning *** paper proposes a transmission-reflection analysis based system using dielectric nanoparticle-doped fiber combined with artificial intelligence to address one of the major problems in the distributed sensing approach:reducing the cost while maintaining high spatial resolution to close the gap between distributed sensors and the general *** learning-based models are designed to classify the perturbed positions when the same force is used and force regression when different forces are applied on each *** results show an accuracy of 99.43%in the position classification of multiple disturbances and an rms error of 1.53 N in the force regression,which represents 5%of the force *** addition,a smart environment using the current system is proposed,which presented 100%accuracy in identifying the positions of different persons in the *** smart environment enables remote home care of patients with high reliability,intelligent decision-making,and a predictive capability.

关键词： smart fiber positions

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Artemisia vulgaris Induces Tumor-Selective Ferroptosis and Necroptosis via Lysosomal Ca^(2+) Signaling

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Chinese Journal of Integrative Medicine 2024年第6期30卷 525-533页

作者： Lucas dos Santos Zamarioli Michele Rosana Maia Santos Adolfo Garcia Erustes Vanessa marques Meccatti Thais Cristine Pereira Soraya S Smaili Maria Cristina Marcucci carlos Rocha Oliveira Gustavo JS Pereira Claudia Bincoletto Department of Pharmacology Paulista School of MedicineFederal University of São PauloSão PauloSP04044-020Brazil Research Group on Phytocomplexes and Cell Signaling School of Health SciencesAnhembi Morumbi UniversitySão PauloSP03164-000Brazil Department of Biosciences and Oral Diagnosis Institute of Science and TechnologySão Paulo State University(ICT-UNESP)São PauloSP12231-280Brazil

Objective To evaluate the chemical composition and effects of Artemisia vulgaris(AV)hydroalcoholic extract(HEAV)on breast cancer cells(MCF-7 and SKBR-3),chronic myeloid leukemia(K562)and NIH/3T3 *** Phytochemical analysis of HEAV was done by high-performance liquid chromatography-mass(HPLC)*** and cell death studies were performed using trypan blue and Annexin/FITC-7AAD,***-1(Fer-1)and necrostatin-1(Nec-1)were used to assess the mode of HEAV-induced cell death and acetoxymethylester(BAPTA-AM)was used to verify the involvement of cytosolic calcium in this *** calcium measurements were made using *** HEAV decreased the viability of MCF-7,SKBR-3 and K562 cells(P<0.05).The viability of HEAV-treated K562 cells was reduced compared to HEAV-exposed fibroblasts(P<0.05).Treatment of K562 cells with HEAV induced cell death primarily by late apoptosis and necrosis in assays using annexin V-FITC/7-AAD(P<0.05).The use of Nec-1 and Fer-1 increased the viability of K562 cells treated with HEAV relative to cells exposed to HEAV alone(P<0.01).HEAV-induced Ca^(2+)release mainly from lysosomes in K562 cells(P<0.01).Furthermore,BAPTA-AM,an intracellular Ca^(2+)chelator,decreased the number of non-viable cells treated with HEAV(P<0.05).Conclusions HEAV is cytotoxic and activates several modalities of cell death,which are partially dependent on lysosomal release of Ca^(2+).These effects may be related to artemisinin and caffeoylquinic acids,the main compounds identified in HEAV.

关键词： Artemisia vulgaris necroptosis ferroptosis late apoptosis Ca^(2+)signaling

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Losartan activates sirtuin 1 in rat reduced-size orthotopic liver transplantation

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World Journal of Gastroenterology 2015年第26期21卷 8021-8031页

作者： Eirini Pantazi Mohamed Bejaoui Mohamed Amine Zaouali Emma Folch-Puy Anabela Pinto Rolo Arnau Panisello carlos marques Palmeira Joan Roselló-Catafau Experimental Pathology Department Experimental Hepatic Ischemia-Reperfusion UnitInstitute of Biomedical Research of Barcelona(IIBB-CSIC Department of Life Sciences Faculty of Science and TechnologyUniversity of Coimbra Portugal and Center for Neurosciences and Cell Biology University of Coimbra

AIM: To investigate a possible association between losartan and sirtuin 1(SIRT1) in reduced-size orthotopic liver transplantation(ROLT) in ***: Livers of male Sprague-Dawley rats(200-250 g) were preserved in University of Wisconsin preservation solution for 1 h at 4 ℃ prior to *** an additional group,an antagonist of angiotensin Ⅱ type 1 receptor(AT1R),losartan,was orally administered(5 mg/kg) 24 h and 1 h before the surgical procedure to both the donors and the ***(as an indicator of liver injury),SIRT1 activity,and nicotinamide adenine dinucleotide(NAD+,a co-factor necessary for SIRT1 activity) levels were determined by biochemical *** expression of SIRT1,acetylated Fox O1(ac-Fox O1),NAMPT(the precursor of NAD+),heat shock proteins(HSP70,HO-1) expression,endoplasmic reticulum stress(GRP78,IRE1 a,p-e IF2) and apoptosis(caspase 12 and caspase 3) parameters were determined by Western *** alterations in protein expression of mitogen activated protein kinases(MAPK),such as p-p38 and p-ERK,were also ***,the SIRT3 protein expression and m RNA levels were ***: The present study demonstrated that losartan administration led to diminished liver injury when compared to ROLT group,as evidenced by the significant decreases in alanine aminotransferase(358.3 ± 133.44 vs 206 ± 33.61,P < 0.05) and aspartate aminotransferase levels(893.57 ± 397.69 vs 500.85 ± 118.07,P < 0.05).The lessened hepatic injury in case of losartan was associated with enhanced SIRT1 protein expression and activity(5.27 ± 0.32 vs 6.08 ± 0.30,P < 0.05).This was concomitant with increased levels of NAD+(0.87 ± 0.22 vs 1.195 ± 0.144,P < 0.05) the co-factor necessary for SIRT1 activity,as well as with decreases in ac-Fox O1 *** treatment also provoked significant attenuation of endoplasmic reticulum stress parameters(GRP78,IRE1 a,p-e IF2) which was consistent with reduced levels of both caspase 12 and caspase 3.

关键词： Losartan Sirtuin 1 Endoplasmic reticulumstress Liver ischemia reperfusion injury Angiotensin Ⅱ

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Multifunctional flexible optical waveguide sensor: on the bioinspiration for ultrasensitive sensors development

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Opto-Electronic Advances 2022年第10期5卷 1-11页

作者： Arnaldo Leal-Junior Leticia Avellar Vitorino Biazi M.Simone Soares Anselmo Frizera carlos marques Graduate Program in Electrical Engineering Federal University of Espírito Santo(UFES)Fernando Ferrari AvenueVitória 29075-910Brazil Mechanical Engineering Department Federal University of Espírito Santo(UFES)Fernando Ferrari AvenueVitória 29075-910Brazil I3N&Physics Department University of AveiroAveiro 3810-193Portugal

This paper presents the development of a bioinspired multifunctional flexible optical sensor(BioMFOS)as an ultrasensitive tool for force(intensity and location)and orientation *** sensor structure is bioinspired in orb webs,which are multifunctional devices for prey capturing and vibration *** multifunctional feature of the structure is achieved by using transparent resins that present both mechanical and optical properties for structural integrity and strain/deflection transmission as well as the optical signal transmission properties with core/cladding configuration of a *** this case,photocurable and polydimethylsiloxane(PDMS)resins are used for the core and cladding,*** optical transmission,tensile tests,and dynamic mechanical analysis are performed in the resins and show the possibility of light transmission at the visible wavelength range in conjunction with high flexibility and a dynamic range up to 150 Hz,suitable for wearable *** BioMFOS has small dimensions(around 2 cm)and lightweight(0.8 g),making it suitable for wearable application and clothing *** tests are performed in the structure by means of applying forces at different locations of the *** results show an ultra-high sensitivity and resolution,where forces in theμN range can be detected and the location of the applied force can also be detected with a sub-millimeter spatial ***,the BioMFOS is tested on the orientation detection in 3D plane,where a correlation coefficient higher than 0.9 is obtained when compared with a gold-standard inertial measurement unit(IMU).Furthermore,the device also shows its capabilities on the movement analysis and classification in two protocols:finger position detection(with the BioMFOS positioned on the top of the hand)and trunk orientation assessment(with the sensor integrated on the clothing).In both cases,the sensor is able of classifying the movement,especially wh

关键词： optical sensors optical waveguides bioinspired design multifunctional structures wearable sensors

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Applying a Context-based Method to Build a Knowledge Graph for the Blue Amazon

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Data Intelligence 2024年第1期6卷 64-103页

作者： Pedro de Moraes Ligabue Anarosa Alves Franco Brandão Sarajane marques Peres Fabio Gagliardi Cozman Paulo Pirozelli Escola Politécnica Universidade de São PauloRua Visconde da Luz60São PauloSão Paulo 05508-010Brazi Escola Politécnica Universidade de São PauloAv.Prof.Luciano Gualbertotv 3158São Paulo 05508-010Brazil Escola de Artes Ciências e Humanidades Universidade de São PauloRua Arlindo Béttio1000São Paulo 03828-000Brazil Instituto de Estudos Avançados Universidade de São PauloRua da Praça do Relógio109São Paulo 05508-050Brazil

Knowledge graphs are employed in several tasks,such as question answering and recommendation systems,due to their ability to represent relationships between *** constructing such a graphs,however,remains an unresolved challenge within knowledge *** tackle this challenge,we propose CtxKG,a method specifically aimed at extracting knowledge graphs in a context of limited resources in which the only input is a set of unstructured text *** is based on OpenIE(a relationship triple extraction method)and BERT(a language model)and contains four stages:the extraction of relationship triples directly from text;the identification of synonyms across triples;the merging of similar entities;and the building of bridges between knowledge graphs of different *** method distinguishes itself from those in the current literature(i)through its use of the parse tree to avoid the overlapping entities produced by base implementations of OpenIE;and(ii)through its bridges,which create a connected network of graphs,overcoming a limitation similar methods have of one isolated graph per *** compare our method to two others by generating graphs for movie articles from Wikipedia and contrasting them with benchmark graphs built from the OMDb movie *** results suggest that our method is able to improve multiple aspects of knowledge graph *** also highlight the critical role that triple identification and named-entity recognition have in improving the quality of automatically generated graphs,suggesting future paths for ***,we apply CtxKG to build BlabKG,a knowledge graph for the Blue Amazon,and discuss possible improvements.

关键词： knowledge graph word embeddings relationship triple extraction Blue Amazon Atlantic Ocean Brazilian coast

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