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Abundance and distribution of meiofauna in the Chukchi Sea

Acta Oceanologica Sinica 2014年第6期33卷 90-94页

作者： LIN Rongcheng HUANG Dingyong GUO Yuqing CHANG Yu cao yinkun WANG Jianjia Third Institute of Oceanography State Oceanic Administration Key Laboratory of Marine Fishery Resources and Ecological Environment of Fujian Province Fisheries CollegeJimei University

The metazoan meiofauna in the Chukchi Sea were collected from seven shallow water stations （depths rang- ing 46 to 52 m） and five deep sea stations （depths ranging between 393 and 2 300 m） during the 4th Chinese National Arctic Research Expedition in 2010. The results showed that abundance of meiofauna was higher in shallow water sediments （average of 2445 ind./（10 cm2）） than in deep sea sediments （407.06 ind./（10 cm2））. A UNIANOVA test for difference between the two different regions was highly significant （F=10h 15, p〈***）. Nematodes were numerically dominant, representing （96.6±4.6）% of the total meiofaunal abundance at the shallow water stations and （98.90±1.42）% at deep sea stations. The number of higher taxonomic groups and abundance of meiofauna were higher at Stas CC1, CC4, and R06 near the Bering Strait and the continent, than at the rest of the shallow water and deep sea stations. The primary factors causing the differences were concentrations of nutrients P and Si of bottom seawater （R=0.831, p〈0.003）, followed by depth （R=-0.655, p〈0.05） and sand fractions of sediments （R=0.632, p 〈0.05）. The numbers of meiofauna on the 65 lam and 32 llm sieves were significantly higher than those on the rest of the screens. Differences in numbers of meiofauna retained on screens with different mesh openings were highly significant among all sampling stations （F=31.60, p〈0.01）. The highest numbers of individuals on screens with 32 μm mesh openings were found at deep sea stations. The number of meiofauna in the top 6-1, 1-2, and 2-4 cm segments constituted 84.4% of the total and was significantly higher than those in the bottom 4-6 and 6-10 cm segments （F=15, p〈0.01）.

关键词： Chukchi Sea meiofauna abundance vertical distribution size spectra deep sea

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A New Critical Nitrogen Dilution Curve for Rice Nitrogen Status Diagnosis in Northeast China

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Pedosphere 2018年第5期28卷 814-822页

作者： HUANG Shanyu MIAO Yuxin cao Qiang YAO yinkun ZHAO Guangming YU Weifeng SHEN Jianning YU Kang Georg BARETH International Center for Agro-Informatics and Sustainable Development College of Resources and Environmental Sciences China Agricultural University Beijing 100193 (China) Institute of Geography University of Cologne K5ln 50923 (Germany) Precision Agriculture Center Department of Soil Water and Climate University of Minnesota St. Paul 55108 (USA) National Engineering and Technology Center for Information Agriculture Nanjing Agricultural University Nanjing 210095 (China) Qixing Farm Jiansanjiang Jiamusi 156399 (China)

In-season diagnosis of crop nitrogen(N) status is crucial for precision N management. Critical N(N_c) dilution curve and N nutrition index(NNI) have been proposed as effective methods to diagnose N status of different crops. The N_c dilution curves have been developed for indica rice in the tropical and temperate zones and japonica rice in the subtropical-temperate zone, but they have not been evaluated for short-season japonica rice in Northeast China. The objectives of this study were to evaluate the previously developed N_c dilution curves for rice in Northeast China and to develop a more suitable N_c dilution curve in this region. A total of17 N rate experiments were conducted in Sanjiang Plain, Heilongjiang Province in Northeast China from 2008 to 2013. The results indicated that none of the two previously developed N_c dilution curves was suitable to diagnose N status of the short-season japonica rice in Northeast China. A new N_c dilution curve was developed and can be described by the equation N_c = 27.7 W^(-0.34) if W ≥ 1 Mg dry matter(DM) ha^(-1) or N_c = 27.7 g kg^(-1) DM if W < 1 Mg DM ha^(-1), where W is the aboveground biomass. This new curve was lower than the previous curves. It was validated using a separate dataset, and it could discriminate non-N-limiting and N-limiting nutritional conditions. Additional studies are needed to further evaluate it for diagnosing N status of different rice cultivars in Northeast China and develop efficient non-destructive methods to estimate NNI for practical applications.

关键词： japonica rice nitrogen nutrition index nitrogen use efficiency plant nitrogen concentration precision nitrogen mana-gement

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Comparison of alternative extraction methods for secretome profiling in human hepatocellular carcinoma cells

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Science China(Life Sciences) 2011年第1期54卷 34-38页

作者： cao Jing SHEN ChengPin ZHANG Jun YAO Jun SHEN HuaLi LIU yinkun LU HaoJie YANG PengYuan Institutes of Biomedical Sciences and Department of Chemistry Fudan University Shanghai 200032 China Liver Cancer Institute Zhongshan Hospital Fudan University Shanghai 200032 China

Secreted proteins are important sources for early detection and diagnosis of disease, and as such have received considerable attention. The extraction of low concentration proteins from large volumes of culture media, which are rich in salts and other compounds that interfere with most proteomics techniques, presents a problem for secretome studies. Ultrafiltration, precipitation, and dialysis are three major extraction methods that can be used to overcome this problem. The present study for the first time, compared the merits and shortcomings of these three methods, without bias. Centrifugal ultrafiltration provided the best extraction efficiency, and precipitation provided the highest number of identifiable proteins. The three methods yielded closely related, but different, information on the secretome; thus, they should be considered complementary or, at least, supplementary methods. Three hundred and sixty unique proteins were identified, including 211 potential secreted proteins. Compared with previous studies, this study also identified 42 new secreted proteins. The present study not only offers a reference for the selection of secretome extraction methods, but also expands the secretome database for the investigation of hepatocellular carcinoma.

关键词： hepatocellular carcinoma secretome protein extraction ultrafiltration precipitation dialysis LC-MS/MS

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早期肝癌甲胎蛋白阴性患者血清标志物的筛选和验证

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中国临床医学 2015年第4期22卷 451-455页

作者：舒宏曹昭张舒甘淋秦雪刘银坤广西医科大学附属肿瘤医院检验科南宁530021 复旦大学肝癌研究所上海200032 广西医科大学第一附属医院检验科南宁530021

目的:筛查甲胎蛋白(α-fetoprotein,AFP)阴性的肝癌患者和肝硬化患者血清中的差异表达蛋白。方法:选择6例AFP阴性的肝癌患者和6例AFP阴性的肝硬化患者。应用MARS系统分离获得各组血清标本中的低丰度蛋白,采用双向荧光差异凝胶电泳(two-d... 详细信息

目的:筛查甲胎蛋白(α-fetoprotein,AFP)阴性的肝癌患者和肝硬化患者血清中的差异表达蛋白。方法:选择6例AFP阴性的肝癌患者和6例AFP阴性的肝硬化患者。应用MARS系统分离获得各组血清标本中的低丰度蛋白,采用双向荧光差异凝胶电泳(two-dimensional fluorescence difference gel electrophoresis,2D-DIGE)技术筛选两组间有表达差异的蛋白,并用蛋白质印迹法加以验证。结果:2D-DIGE技术共鉴定出29种差异蛋白,其中AFP阴性肝癌患者血清中高表达的蛋白有22种,低表达的蛋白有7种。差异蛋白包括激肽原1、四连接素、基膜聚糖蛋白、补体1r和锌α2糖蛋白等。蛋白质印迹法证实,AFP阴性的肝癌患者血清中锌α2糖蛋白表达水平明显低于AFP阴性的肝硬化患者(P<0.05),同DIGE结果一致。结论:采用蛋白质组学技术筛查出的一系列在AFP阴性肝硬化和AFP阴性肝癌患者血清中有表达差异的蛋白有望作为AFP阴性肝癌患者早期诊断和辅助诊断的分子标志物。

关键词：肝肿瘤肝硬化双向荧光差异凝胶电泳蛋白质印迹法甲胎蛋白

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基于数据挖掘的血液病从虚劳论治的证治规律研究

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世界科学技术-中医药现代化 2021年第10期23卷 3821-3829页

作者：张知睿许银坤卢伟名李玥鑫刘诤曹晋丁富平蔡毅张进中山大学生物科学类理工实验班广州510275 广州中医药大学研究生院广州510006 华南理工大学软件学院广州510641 广州中医药大学护理学院广州510006 广州中医药大学基础医学院广州510006

目的探讨中医药从虚劳论治血液病的证治规律,为临床治疗的运用与相关药物研发提供必要的参考依据。方法通过检索CNKI、CBM、维普、万方等数据库,收集整理相关文献,建立中医治疗虚劳性血液病证治信息数据库。运用Excel 2013软件进行描述... 详细信息

目的探讨中医药从虚劳论治血液病的证治规律,为临床治疗的运用与相关药物研发提供必要的参考依据。方法通过检索CNKI、CBM、维普、万方等数据库,收集整理相关文献,建立中医治疗虚劳性血液病证治信息数据库。运用Excel 2013软件进行描述性分析,以R语言软件和Python软件开展关联规则和聚类分析。结果血液病的主要症状为乏力、头晕、心悸、气短、腰膝酸软等等,舌象主要表现为舌淡、舌红、苔薄、苔白等等,脉象主要表现为脉细、脉数、脉沉、脉弱等等。从虚劳论治血液病最常使用的药物为补气药类、补血药类、滋阴药类和活血化瘀药类。用药以温平为主,药味以甘味(49.99%)、苦味(21.24%)和辛味(12.68%)最为常用。常用的补气药类有黄芪、白术、党参和茯苓等,常用的补血药类有当归、熟地黄、鸡血藤和白芍等,常用的滋阴药类有熟地黄、女贞子、阿胶和墨旱莲等,常用的活血化瘀药类有生地黄、丹参、牡丹皮和赤芍等。关联支持度最高为黄芪和人参,其次为女贞子和墨旱莲,符合气虚和阴虚的病机。聚为4类,以补虚药和活血药两类为主。结论血液病从虚劳论治,症状舌脉符合气虚和阴虚病机,治疗以补气滋阴药物为主。

关键词：虚劳血液病证治规律关联规则数据挖掘

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荆防颗粒挥发油β-环糊精制备工艺的优化

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环球中医药 2023年第5期16卷 875-880页

作者：曹玲李因坤崔琳琳高艳红姜佳峰鲁南制药集团股份有限公司中药制药共性技术国家重点实验室临沂276006 鲁南厚普制药有限公司

目的优化荆防颗粒挥发油β-环糊精包合物制备工艺。方法以挥发油包合率、包合物得率为评价指标,比较胶体磨法、饱和水溶液法、超声法包合物制备工艺,确定包合方法;在单因素实验的基础上,采用Box-Behnken响应面法,以挥发油包合率、包合... 详细信息

目的优化荆防颗粒挥发油β-环糊精包合物制备工艺。方法以挥发油包合率、包合物得率为评价指标,比较胶体磨法、饱和水溶液法、超声法包合物制备工艺,确定包合方法;在单因素实验的基础上,采用Box-Behnken响应面法,以挥发油包合率、包合物得率的综合评分为考察指标,考察挥发油与β-环糊精的比例(1∶6~1∶10)、β-环糊精与水的比例(1∶2~1∶4)以及研磨包合时间(15~25分钟)对包合物制备工艺的影响,通过气相色谱法对包合前后挥发油化学成分进行检测。结果经对比,胶体磨法得到的包合物挥发油包合率(80.37%)和包合物得率(86.55%)均高于饱和水溶液法(75.19%、79.49%)和超声法(68.38%、72.05%),故选择胶体磨法制备荆防颗粒挥发油β-环糊精包合物;优选出的最佳工艺条件为挥发油∶β-环糊精为1∶8,β-环糊精∶水为1∶3,研磨包合时间为21分钟,3次验证实验结果显示,挥发油包合率平均为85.98%,包合物得率平均为92.51%。结论优选的荆防颗粒挥发油包合工艺包合率和包合物得率均较高,包合工艺科学、合理、可行。

关键词：荆防颗粒挥发油 β-环糊精 Box-Behnken响应面法胶体磨法饱和水溶液法超声法包合工艺

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