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Fighting hypoxia to improve photodynamic therapy-driven immunotherapy: Alleviating, exploiting and disregarding

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Chinese Chemical Letters 2025年第2期36卷 57-68页

作者： Liangliang Jia Ye hong Xinyu He Ying Zhou Liujiao Ren hongjun Du Bin Zhao Bin Qin zhe Yang Di Gao The Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and Technology Xi'an Jiaotong University Center of Digestive Endoscopy Shaanxi Provincial Cancer Hospital Department of Ophthalmology Xijing Hospital Department of Epidemiology Shaanxi Provincial Cancer Hospital Department of Gastroenterology Second Affiliated Hospital Xi'an Jiaotong University Research Institute of Xi'an Jiaotong University

Innovative anti-cancer therapies that activate the immune system show promise in combating cancers resistant to conventional treatments. Photodynamic therapy (PDT) is one such treatment, which not only directly eliminates tumor cells but also functions as an in situ tumor vaccine by enhancing tumor immunogenicity and triggering anti-tumor immune responses through immunogenic cell death (ICD). However, the effectiveness of PDT in enhancing immune responses is influenced by factors, such as photosensitizers and the tumor microenvironment, particularly hypoxia. Current clinically used PDT heavily relies on oxygen (O2) availability and can be limited by tumor hypoxia. Additionally, the tumor immunosuppressive microenvironment induced by hypoxia affects the anti-tumor immunity of tumor-infiltrating effector T cells. Meanwhile, the immunosuppressive myeloid-lineage cells are recruited to the hypoxic tumor tissue and exhibit higher immunosuppressive capabilities under hypoxia conditions. Consequently,numerous strategies have been developed to modulate tumor hypoxia or to create hypoxia-compatible PDT, aiming to reduce the effects of tumor hypoxia on PDT-driven immunotherapy. This review investigates these strategies, including approaches to alleviate, exploit, and disregard tumor hypoxia within the context of PDT/immunotherapy. It also emphasizes the role of advanced nanomedicine and its benefits in these strategies, while outlining current challenges and future prospects in the field.

关键词： Immunogenic cell death Immunotherapy Nanomedicine Photodynamic therapy Photosensitizers Tumor hypoxia

Transmembrane protein 176B promotes epithelial-mesenchymal transition in colorectal cancer through inflammasome inhibition

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World Journal of Gastrointestinal Oncology 2025年第3期17卷 171-187页

作者： Wei Qian Chong-Yi Xu Wei hong zhe-Ming Li Dao-Gun Xu Department of Proctology Wenling Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medical UniversityWenling 317500Zhejiang ProvinceChina College of Life Sciences Zhejiang Chinese Medical UniversityHangzhou 310000Zhejiang ProvinceChina

BACKGROUND Activation of the epithelial-mesenchymal transition(EMT),a pivotal process in tumor metastasis and evasion,as well as the NLRP3 inflammasome,both promote colorectal cancer(CRC)*** studies have shown that Transmembrane protein 176B(TMEM176B)regulates NLRP3 and promotes CRC malignant *** To investigate the role of TMEM176B in modulating NLRP3 inflammasome and its implications on EMT and tumor progression in *** CRC in situ mouse and co-cultured cell models were established using CT26 cells,BALB/c mice,and primary cultured mouse natural killer(NK)*** hairpin RNA knocked down TMEM176B and NLRP3 expression in CT26 *** imaging,Terminal deoxynucleotidyl transferase dUTP nick end labeling assays,immunohistochemistry staining,flow cytometry,and molecular assays were used to investigate the effects of TMEM176B knockdown on the NLRP3 inflammasome in NK cells to assess tumor metastasis,apoptosis,and EMT *** Silencing TMEM176B in CRC mice significantly reduced tumor metastasis,proliferation,and EMT,while activating apoptosis,NLRP3 inflammasome,and NK cell ***,silencing TMEM176B in co-cultured cell models inhibited cell migration and invasion,and promoted *** interference of NLRP3 reversed these effects by modulating key proteins such as phosphorylated nuclear factor kappa B subunit 1 p65,matrix metallopeptidase 9,and transforming growth factor-β.CONCLUSION This study highlights the critical role of TMEM176B/NLRP3 in CRC progression and provides a basis for targeting this axis as a novel therapeutic approach to manage CRC progression and metastasis.

关键词： Transmembrane protein 176B Epithelial-mesenchymal transition Colorectal cancer Pyrin domain containing 3 inflammasome Natural killer cell

Predicting the efficiency of arsenic immobilization in soils by biochar using machine learning

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Journal of Environmental Sciences 2025年第1期147卷 259-267页

作者： Jin-Man cao Yu-Qian Liu Yan-Qing Liu Shu-Dan Xue Hai-hong Xiong Chong-Lin Xu Qi Xu Gui-Lan Duan State Key Lab of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China Henan Institute of Advanced Technology Zhengzhou UniversityZhengzhou 450052China University of Chinese Academy of Sciences Beijing 100049China

Arsenic(As)pollution in soils is a pervasive environmental *** immobilization offers a promising solution for addressing soil As *** efficiency of biochar in immobilizing As in soils primarily hinges on the characteristics of both the soil and the ***,the influence of a specific property on As immobilization varies among different studies,and the development and application of arsenic passivation materials based on biochar often rely on empirical *** enhance immobilization efficiency and reduce labor and time costs,a machine learning(ML)model was employed to predict As immobilization efficiency before biochar *** this study,we collected a dataset comprising 182 data points on As immobilization efficiency from 17 publications to construct three ML *** results demonstrated that the random forest(RF)model outperformed gradient boost regression tree and support vector regression models in predictive *** importance analysis and partial dependence plots based on the RF model were conducted to identify the most crucial factors influencing As *** findings highlighted the significant roles of biochar application time and biochar pH in As immobilization efficiency in ***,the study revealed that Fe-modified biochar exhibited a substantial improvement in As *** insights can facilitate targeted biochar property design and optimization of biochar application conditions to enhance As immobilization efficiency.

关键词： Biochar Arsenic immobilization Soil Machine learning

Assessing the possibility of using large language models in ocular surface diseases

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International Journal of Ophthalmology(English edition) 2025年第1期18卷 1-8页

作者： Qian Ling Zi-Song Xu Yan-Mei Zeng Qi hong Xian-zhe Qian Jin-Yu Hu Chong-Gang Pei hong Wei Jie Zou Cheng Chen Xiao-Yu Wang Xu Chen zhen-Kai Wu Yi Shao Department of Ophthalmology the First Affiliated HospitalJiangxi Medical CollegeNanchang UniversityNanchang 330006Jiangxi ProvinceChina Ophthalmology Centre of Maastricht University Maastricht 6200MSLimburgNetherlands Changde Hospital Xiangya School of MedicineCentral South University(the First People’s Hospital of Changde City)Changde 415000Hunan ProvinceChina Department of Ophthalmology Shanghai General HospitalShanghai Jiao Tong University School of MedicineNational Clinical Research Center for Eye DiseasesShanghai 200080China

AIM:To assess the possibility of using different large language models(LLMs)in ocular surface diseases by selecting five different LLMS to test their accuracy in answering specialized questions related to ocular surface diseases:ChatGPT-4,ChatGPT-3.5,Claude 2,PaLM2,and ***:A group of experienced ophthalmology professors were asked to develop a 100-question singlechoice question on ocular surface diseases designed to assess the performance of LLMs and human participants in answering ophthalmology specialty exam *** exam includes questions on the following topics:keratitis disease(20 questions),keratoconus,keratomalaciac,corneal dystrophy,corneal degeneration,erosive corneal ulcers,and corneal lesions associated with systemic diseases(20 questions),conjunctivitis disease(20 questions),trachoma,pterygoid and conjunctival tumor diseases(20 questions),and dry eye disease(20 questions).Then the total score of each LLMs and compared their mean score,mean correlation,variance,and confidence were ***:GPT-4 exhibited the highest performance in terms of *** the average scores of the LLMs group with the four human groups,chief physician,attending physician,regular trainee,and graduate student,it was found that except for ChatGPT-4,the total score of the rest of the LLMs is lower than that of the graduate student group,which had the lowest score in the human *** ChatGPT-4 and PaLM2 were more likely to give exact and correct answers,giving very little chance of an incorrect ***-4 showed higher credibility when answering questions,with a success rate of 59%,but gave the wrong answer to the question 28% of the ***:GPT-4 model exhibits excellent performance in both answer relevance and ***2 shows a positive correlation(up to 0.8)in terms of answer accuracy during the *** terms of answer confidence,PaLM2 is second only to GPT4 and surpasses Claude 2,SenseNova,and *** the fact t

关键词： ChatGPT-4.0 ChatGPT-3.5 large language models ocular surface diseases

鄂尔多斯盆地华池地区三叠系长7页岩油储层特征、形成环境及富集模式

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岩性油气藏 2025年第1期37卷 24-40页

作者：梁锋曹哲延长油田股份有限公司吴起采油厂陕西延安717600

通过对鄂尔多斯盆地华池地区三叠系长7段钻井岩心进行岩石学特征以及元素地球化学测试分析,采用V/(V+Ni),Ni/Co,U/Th,Sr/Cu,cao/(MgO*Al_(2)O_(3)),Sr/Ba,Fe/Mn,Mn/Ti,Ba_(bio)值和P/Al值等多种判别标准,并结合总有机碳的含量,进行古环... 详细信息

通过对鄂尔多斯盆地华池地区三叠系长7段钻井岩心进行岩石学特征以及元素地球化学测试分析,采用V/(V+Ni),Ni/Co,U/Th,Sr/Cu,cao/(MgO*Al_(2)O_(3)),Sr/Ba,Fe/Mn,Mn/Ti,Ba_(bio)值和P/Al值等多种判别标准,并结合总有机碳的含量,进行古环境分析,恢复了沉积期的古气候、古盐度和古氧化还原条件等。研究结果表明:①鄂尔多斯盆地华池地区三叠系长7段岩石主要为深灰色泥岩、深灰色灰质泥岩及含炭屑的粉砂质泥岩、富含有机质的粉砂质泥岩,黏土矿物晶间孔与粒间孔是主要的孔隙类型,可见少量溶蚀孔和微裂缝。②研究区延长组长7沉积时期,有机质生产力强,环境以淡水为主且处于贫氧到缺氧状态,其中长7_(2)—长7_(3)亚段沉积时期,部分水体较深的区域出现极强的还原条件,主要为温湿气候。整体上为深水环境,长7_(2)亚段沉积时位于深水区的边界地带,其水深明显较长71与长7_(3)亚段更深,P/Al值以及Ba_(bio)值总体上变化趋势一致,自下而上具有逐渐增大的趋势。③研究区延长组长7段古沉积环境具备适宜的温度和盐度条件,与深水和强还原性特征共同促进了有机质的广泛发育、富集和保存,源岩与储层之间通过裂缝连通,离源岩较近的储层,其捕获原油的数量和效率更高,这种源储配置为页岩油的大规模富集成藏奠定了坚实的基础。

关键词：页岩油储层元素地球化学古环境古生产力有机质富集源储配置长7 三叠系华池地区鄂尔多斯盆地

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Positron annihilation study of defect formation and evolution in matrix graphite under He ion irradiation

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Nuclear Science and Techniques 2025年第1期36卷 13-21页

作者： hong-Xia Xu Jian-Dang Liu Bang-Jiao Ye Zi-Wen Pan Jun Lin Jin-Liang Song Jian-Qing cao Chao Yan Ying-Ping Hao Jin-Xing Cheng Qing-Bo Wang Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China State Key Laboratory of Particle Detection and Electronics University of Science and Technology of ChinaHefei 230026China Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200025China Beijing Institute of High Technology Beijing 100094China

The stability of matrix graphite under neutron irradiation and in corrosive environments is crucial for the safe operation of molten salt reactors(MSRs).Raman spectroscopy and a slow positron beam were employed to investigate the effects of He ion irradiation fluences and subsequent annealing on the microstructure and defects of the matrix *** ions with 500 keV energy and fluences ranging from 1.1×10^(15)ions∕cm^(2)to 3.5×10^(17)ions∕cm^(2)were used to simulate neutron irradiation at 300 *** samples with an irradiation fluence of 3.5×10^(16)ions∕cm^(2)were subjected to isochronal annealing at different temperatures(573 K,873 K and 1173 K)for 3 *** Raman results revealed that the D peak gradually increased,whereas the intrinsic G peak decreased with increasing irradiation *** the same irradiation fluence,the D peak gradually decreased,whereas the intrinsic G peak increased with increasing annealing *** positron beam analysis demonstrated that the density or size of irradiation defects(vacancy type)increased with higher irradiation fluence,but decreased rapidly with increasing annealing *** Raman spectral analysis of sample cross sections subjected to high irradiation fluences revealed the emergence of amorphization precisely at the depth where ion damage was most pronounced,whereas the surface retained its crystalline *** and positron annihilation analyses indicated that the matrix graphite exhibited good irradiation resistance to He ions at 300 ***,vacancy-type defects induced by He ion irradiation exhibit poor thermal stability and can be easily removed during annealing.

关键词： Graphite Positron annihilation Irradiation Raman spectrum

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江西省海外客源市场空间结构研究

经济研究导刊 2011年第27期 141-143页

作者：许庆勇曹红哲南昌大学旅游规划与研究中心南昌330031 南昌大学理学院南昌330031

旅游地客源市场空间结构对旅游业发展具有重要影响。通过研究2001—2010年江西省海外主要客源市场数据,分析了海外客源市场的集聚程度,得出江西省海外旅游市场所呈现的一些规律;探讨了江西海外旅游市场是否具有稳定性,并提出江西省在发... 详细信息

旅游地客源市场空间结构对旅游业发展具有重要影响。通过研究2001—2010年江西省海外主要客源市场数据,分析了海外客源市场的集聚程度,得出江西省海外旅游市场所呈现的一些规律;探讨了江西海外旅游市场是否具有稳定性,并提出江西省在发展海外市场的一些对策。

关键词：江西海外客源市场空间结构

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玉米SPXs家族基因的鉴定与表达规律分析

植物病理学报 2021年第3期51卷 340-350页

作者：藏金萍朱倩洁邢超曹宏哲董金皋张康邢继红省部共建华北作物改良与调控国家重点实验室保定071000 河北农业大学生命科学学院保定071000 河北省植物生理与分子病理学重点实验室保定071000

SPXs(SPX-domain-containing proteins)家族基因在真核生物无机磷酸盐(Pi)的传感、信号转导和转运中发挥着重要作用,但玉米SPXs家族基因的相关研究未见报道。本研究利用生物信息学手段鉴定了15个玉米SPXs家族基因,通过系统进化和保守结... 详细信息

SPXs(SPX-domain-containing proteins)家族基因在真核生物无机磷酸盐(Pi)的传感、信号转导和转运中发挥着重要作用,但玉米SPXs家族基因的相关研究未见报道。本研究利用生物信息学手段鉴定了15个玉米SPXs家族基因,通过系统进化和保守结构域分析将其分为SPX-EXS、SPX、SPX-Zinc finger和SPX-MFS 4个亚家族。通过表达谱数据分析,发现ZmSPXs家族基因具有明显的组织特异性表达。同时,我们还发现ZmSPXs家族基因在生物胁迫和非生物胁迫过程中表达模式也具有很大差异,说明这些基因可能在不同的生物过程中发挥重要作用。最后,利用荧光实时定量PCR技术,对玉米SPXs家族基因在磷胁迫处理下的表达规律进行分析,结果发现大部分基因在磷胁迫处理下呈现高水平表达。本研究鉴定了玉米SPXs家族基因,明确了该家族基因的系统进化关系、保守结构域、组织表达特性以及在生物和非生物胁迫条件下的表达规律,为阐明玉米SPXs家族基因的功能及其机制奠定了理论基础。

关键词：玉米 SPXs 系统进化分析基因表达磷胁迫

禾谷镰孢菌过氧化物酶家族基因的表达规律分析

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农业生物技术学报 2019年第6期27卷 1090-1100页

作者：柳健虎张康藏金萍曹宏哲张靖邢继红董金皋河北农业大学生命科学学院/河北省植物生理与分子病理学重点实验室/河北农业大学真菌毒素与植物分子病理学实验室

过氧化物酶作为病原真菌抗氧化防御系统的主要组分,能够清除来源于植物体产生的活性氧(reactive oxygen species, ROS),促使病原菌成功侵染宿主植物细胞,禾谷镰孢菌(Fusarium graminearum)过氧化物酶(peroxidase,POX)的相关研究尚未见... 详细信息

过氧化物酶作为病原真菌抗氧化防御系统的主要组分,能够清除来源于植物体产生的活性氧(reactive oxygen species, ROS),促使病原菌成功侵染宿主植物细胞,禾谷镰孢菌(Fusarium graminearum)过氧化物酶(peroxidase,POX)的相关研究尚未见报道。本研究利用生物信息学手段搜索真菌过氧化物酶基因的数据库,获得了31个禾谷镰孢菌过氧化物酶基因,系统进化分析和保守结构域分析均将其分为18个亚家族。对过氧化物酶家族基因的表达模式进行分析,发现FgPOX (禾谷镰孢菌过氧化物酶, Fusarium graminearum peroxidase)-13、FgPOX-17、FgPOX-16、FgPOX-19等基因在菌丝和分生孢子中均具有较高表达水平,FgPOX-24、FgPOX-29在菌丝中表达水平较高,FgPOX-11、FgPOX-10、FgPOX-4、FgPOX-23在分生孢子中表达水平较高。对过氧化物酶家族基因在侵染过程中的表达规律进行分析,发现FgPOX-13在病菌侵染过程中表达水平明显升高并保持较高水平,FgPOX-17、FgPOX-28、FgPOX-30等基因在病菌侵染的早期表达水平较高,FgPOX-12、FgPOX-10、FgPOX-23等基因在病菌侵染的后期表达水平较高。进一步利用qRT-PCR技术对禾谷镰孢菌过氧化物酶家族基因在H2O2胁迫条件下表达规律进行分析,发现FgPOX-1、FgPOX-4、FgPOX-6、FgPOX-9、FgPOX-10、FgPOX-22、FgPOX-30的表达水平是正常条件下的20倍以上,FgPOX-9、FgPOX-13等随着H2O2胁迫时间的延长,表达水平明显增高,而FgPOX-1、FgPOX-21等随着H2O2胁迫时间的延长,表达水平明显降低。本研究明确了禾谷镰孢菌过氧化物酶家族基因数量、系统进化关系、保守结构域,以及在病菌不同组织、侵染过程和响应H2O2胁迫中的表达规律,为阐明禾谷镰孢菌过氧化物酶家族基因的功能提供了基础资料。

关键词：禾谷镰孢菌过氧化物酶家族基因系统进化基因表达