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Detecting peroxiredoxin hyperoxidation by one-dimensional isoelectric focusing

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Biophysics Reports 2015年第1期1卷 14-17页

作者： zhenbo cao Neil J.Bulleid Institute of Molecular Cellular and Systems BiologyCollege of Medical Veterinary and Life SciencesDavidson BuildingUniversity of Glasgow

The activity of typical 2-cys peroxiredoxin(Prxs) can be regulated by hyperoxidation with a consequent loss of redox activity. Here we developed a simple assay to monitor the level of hyperoxidation of different typical 2-cys prxs simultaneously. This assay only requires standard equipment and can compare different samples on the same gel. It requires much less time than conventional 2 D gels and gives more information than Western blotting with an antibody specific for hyperoxidized peroxiredoxin. This method could also be used to monitor protein modification with a charge difference such as phosphorylation.

关键词： Hyperoxidation Isoelectric focusing Peroxiredoxin

Preparation of the selenome-thionine derivative of tab-toxin resistance protein

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Chinese Science Bulletin 2001年第15期46卷 1304-1307页

作者： HE Hongzhen SHAO Yu CHEN Yuhang LIU Bingbing cao zhenbo JIANG Fan LIU Yiwei TANG Hong LIU Jinyuan ZHAO Nanming RAO Zihe Laboratory of Structural Biology&Protein Science Laboratory of MOE School of Life Science and EngineeringTsinghua UniversityBeijing 100084China

In order to obtain phase information for the X-ray diffraction of tabtoxin resistance protein (TTR) crystal using the MAD phasing method, a selenomethionine (Se-Met) derivative of TTR was overexpressed in E. coli strain M15, with pQE-30 plasmid, through IPTG induction in M9 medium containing Se-Met. The product was purified to an estimated homogeneity of greater than 95% according to SDS-PAGE, by a Ni-NTA metal affinity followed by a Mono Q anion exchange column chromatography. The successful substitution of Se-Met for methionine (Met) was confirmed by MALDI-TOF and ESI-Quadrupole Mass Spectrometry analysis. The derivative crystal was obtained using similar conditions as those for the native.

关键词： Se-Met TTR derivative expression purification mass spectrometry.

Synthesis and Characterization of Triarylamine-Based Block Copolymers by Combination of C-N Coupling and ATRP for Photorefractive Applications

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Open Journal of Organic Polymer Materials 2012年第4期2卷 53-62页

作者： zhenbo cao Kousuke Kousuke Tsuchiy Takeshi Shimomura Kenji Ogino Graduate School of Bio-Applications and Systems Engineering Tokyo University of Agriculture and Technology Tokyo Japan

Poly(4-butyltriarylamine)s with t-butyldimethylsilyl terminal protecting group (PBTPA-TBS) with various molecular weights were prepared by C-N coupling polymerization. The resulting precursors were postfunctionalized and subse- quently used as macroinitiators for atom transfer radial polymerization (ATRP) of n-butyl acrylate (n-BA) and ethyl acrylate (EA). Both the polymerization processes were controlled and the polymers were characterized by 1H NMR, gel permeation chromatography (GPC) and thermal properties, which confirmed the successful synthesis of all the poly-mers. The microphase separated behaviors of the poly (4-butyltriarylamine)-block-poly (butyl acrylate) (PBTPA-b-PBA) were examined by AFM in the film showing phase separation structures for all the polymers. The photorefractive property of the composite based on PBTPA-b-PBA block copolymer was evaluated by two-beam coupling experiment. A relative high gain coefficient of 42.7 cm?1 was obtained at the electric field of 31 V/?m.

关键词： C-N Coupling Atrp Triarylamine Acrylate Ester Photorefractivity

Preparation and Properties of Glass with High Transmittance and Intense Gamma Ray Shielding

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硅酸盐学报 2024年第10期52卷 3301-3310页

作者： ZHANG Meilun cao zhenbo YANG Shengyun ZHANG Yang HAN Yu QIU Fu ZHOU You ZHENG Jingming LIU Hui JIA Jinsheng China National Building Material Photonics Technology Co. Ltd.Zaozhuang 277000ShandongChina China Building Materials Academy Co. Ltd.Beijing 100000China

Introduction As a kind of high-energy electromagnetic radiation,gamma rays can lead to radiation effect and environmental *** ensure the reliability and safety utilization of gamma radiations in different areas,materials with reliable gamma shielding performances should be *** existing efforts are to use glasses as alternative for the conventional radiation shielding *** a typical window material,glass itself has attracted wide attention due to its unique chemical and physical *** them,lead-based radiation shielding materials are widely used in the field of ionizing radiation protection due to their reliable physical *** the nuclear industry and high energy physics laboratories,different materials can be used for temporary or permanent *** lead-based glass doped with heavy metal oxide PbO is widely used in the field of radiation shielding for a long time with reliable light transmission properties,which facilitates the observation and monitoring of radiation *** this paper,three kinds of transparent silicate glass with different PbO contents were prepared by a high-temperature melting forming *** gamma-rays protection abilities of the three kinds of glass were investigated through experimental and thermotical *** chemical and thermal stability of the glass were also *** addition,the influence mechanism of the structure and performance differences of the three kinds of glass was also *** Analytical pure quartz sand(SiO_(2),purity 99.5%,in mass,the same below),lead monoxide(PbO,purity 99.0%),potassium nitrate(KNO3,purity 99.0%)and sodium nitrate(NaNO3,purity 99.0%)were selected as raw materials for preparation of three kinds of gamma-rays shielding glass materials by a high-temperature melting *** appropriate amount of raw materials was thoroughly mixed and added to 3 L quartz crucible and heated in air at 1200℃.Then the raw materials were melted at 1250℃for 14

关键词： transmittance gamma rays radiation shielding glass

身体活动对血液循环维生素D水平的影响--对观察性和实验性研究的系统综述与meta分析

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上海体育学院学报 2021年第10期45卷 81-96页

作者：张景华王柯曹振波上海体育学院运动科学学院上海200438

目的系统梳理身体活动对血液循环维生素D水平影响的观察性研究和实验性研究,为身体活动调节微量营养素代谢提供理论依据。方法检索自数据库建立至2020年11月30日PubMed、Embase、CochraneLibrary、中国知网等数据库中的观察性研究和实... 详细信息

目的系统梳理身体活动对血液循环维生素D水平影响的观察性研究和实验性研究,为身体活动调节微量营养素代谢提供理论依据。方法检索自数据库建立至2020年11月30日PubMed、Embase、CochraneLibrary、中国知网等数据库中的观察性研究和实验性研究。根据纳入和排除标准筛选文献,并进行meta分析和系统综述。结果共纳入74篇文献,包括51篇观察性研究和23篇实验性研究。观察性研究meta分析结果显示,较高的身体活动水平与较低的维生素D缺乏风险显著相关。亚组分析结果表明:室外身体活动会显著降低维生素D缺乏风险,但室内身体活动无该效应;较高强度的身体活动与较低的维生素D缺乏风险显著相关,但低强度身体活动与之无相关性;不同季节身体活动均与较低的维生素D缺乏风险显著相关。实验性研究结果表明,急性运动和长期运动训练均可显著提高血液循环25-羟维生素D[25(OH)D]水平;亚组分析结果显示,耐力训练和抗阻联合耐力训练均能显著提高血清25(OH)D水平,但抗阻训练并无显著性效应。结论身体活动水平与血液循环维生素D水平之间呈显著正相关,且运动干预对血液循环维生素D营养状态具有有益作用。

关键词：运动身体活动 25-羟维生素D[25(OH)D] meta分析

大学生久坐行为/屏幕时间与心理健康的Meta分析

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中华流行病学杂志 2023年第3期44卷 477-485页

作者：高雪蕾张景华杨漾曹振波上海理工大学体育教学部上海200093 上海体育学院运动健康学院上海200438 上海市学生体质健康研究中心上海200438

目的系统梳理久坐行为/屏幕时间与大学生心理健康影响的相关文献,旨在探讨大学生久坐行为/屏幕时间与心理健康之间的关系,为促进大学生心理健康发展提供理论依据。方法在PubMed、Embase、Cochrane Library、中国知网、维普数据库和万方... 详细信息

目的系统梳理久坐行为/屏幕时间与大学生心理健康影响的相关文献,旨在探讨大学生久坐行为/屏幕时间与心理健康之间的关系,为促进大学生心理健康发展提供理论依据。方法在PubMed、Embase、Cochrane Library、中国知网、维普数据库和万方数据知识服务平台进行检索,截止日期为2022年7月14日,研究久坐行为/屏幕时间与大学生心理健康研究相关文献。由一名研究者按照拟订方案对纳入的文献进行数据提取并进行质量评分,再由另一名研究者进行复核;对符合纳入标准的文献进行系统综述,并根据文献可提取数据的情况,采用Stata 14.2软件进行Meta分析。结果共有36篇文献符合纳入标准,包括35篇观察性研究和1篇干预性研究。对久坐行为(4篇)、屏幕时间(9篇)与大学生抑郁、屏幕时间与大学生焦虑(4篇)进行Meta分析,对其他研究进行系统综述。久坐行为与大学生抑郁的Meta分析结果显示,较长的久坐时长增加了大学生抑郁风险(OR=1.07,95%CI:1.05~1.10)。亚组分析显示,未校正混杂因素模型中,较长的久坐时长与抑郁不存在相关性(OR=1.74,95%CI:0.93~3.25),但校正混杂因素后两者之间呈正相关(OR=2.15,95%CI:1.18~3.90)。屏幕时间与大学生抑郁的Meta分析结果显示,较长的屏幕时间与抑郁呈显著正相关(OR=1.03,95%CI:1.02~1.05)。亚组分析结果显示,未校正混杂因素模型和校正混杂因素模型中,较长的屏幕时间与抑郁均呈显著正相关(OR=1.27,95%CI:1.13~1.42;OR=1.45,95%CI:1.18~1.79)。久坐行为与大学生焦虑的Meta分析显示,较长的屏幕时间与较高的焦虑风险呈正相关(OR=1.44,95%CI:1.31~1.58)。亚组分析结果显示,原始模型和校正混杂因素模型中,较长屏幕时间与焦虑呈正相关(OR=1.47,95%CI:1.31~1.65;OR=1.38,95%CI:1.17~1.62)。对不符合Meta分析的文献综述发现,久坐行为/屏幕时间与大学生压力和其他心理健康具有显著相关性。结论久坐行为或屏幕时间与大学生心理健康呈显著负相关,特别是对大学生抑郁和焦虑情绪具有显著影响,且该影响可能存在学习日和休息日差异。

关键词：大学生久坐行为屏幕时间心理健康 Meta分析