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Decreased Pollen Viability and Thicken Pollen Intine in Antisense Silenced Brassica campestris Mutant of BcMF19

Journal of Integrative Agriculture 2014年第5期13卷 954-962页

作者： LIU Jin-long GAO Ming-hui LIU Ying cao jia-shu Laboratory of Cell and Molecular Biology Institute of Vegetable Science Zhejiang University

Brassica campestris male fertility 19 （BcMF19; GenBank accession number GQ902048.1）, a gene that is specially expressed in tapetum cells and microspores during anther development in B. campestris ssp. chinensis, which is learned from the previous in situ hybridization study. In the present study, we constructed antisense-silenced plants of BcMF19 using B. campestris ssp. chinensis to validate this prediction. The morphology of the pistils, long anthers, and short anthers was significantly affected in 35sbcmf19 compared with the control samples. 4＇-6-Diamidino-2-phenylindole staining revealed that two generative nuclei and one large vegetative nucleus were not affected in the mutant compared with control. Statistical analysis of Alexander＇s staining results showed that 96% of the control pollen grains had vitality, whereas only 86% of the mutant pollen grains did. Under scanning electron microscopy, the mutant demonstrated numerous abnormal pollen grains and resembled dried persimmon. The frequency of normal pollen grains was approximately 18%. Under transmission electron microscopy, the pollen intine during the binucleate and mature pollen stages in 35sbcmf19 exhibited abnormal thickening, especially at the germinal furrows, compared with control. In vitro pollen germination test showed that the tips of the mutant pollen tubes transformed into globular alveoli and stopped growing compared with control. On the other hand, in vivo pollen germination test suggested that BcMF19 affected the pollen tube extension in the pistil. These findings indicate that BcMF19 is essential to the pollen development and pollen tube extension orB. campestris ssp. chinensis.

关键词： Brassica rapa ssp. chinensis PGIP pollen polygalacturonase-inhibiting protein tapetum pollen germination

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Construction of a New Molecular Barcode for Discriminating Plants and Animals with a Close Genetic Relationship

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Journal of Integrative Agriculture 2013年第7期12卷 1138-1151页

作者： HUANG Li ZHAO Xiao-feng ZHU Yi-peng DONG Heng XU Ning-ying cao jia-shu Laboratory of Cell & Molecular Biology Institute of Vegetable Science Zhejiang University Key Laboratory of Horticultural Plant Growth Development and Quality Improvement Ministry of Agriculture College of Life and Environment Sciences Hangzhou Normal University College of Animal Science Zhejiang University

DNA barcodes have been proposed as a shortcut to provide species identification and as a way to accelerate the discovery of new species. A number of candidate gene regions have been suggested as possible barcodes for animals and plants, but for the identification of recently diverged species and/or varieties with only a few genetic differences it has been reported to be problematic in some cases. This study selected widely cultivated cruciferous vegetables as the primary samples, after failure of discrimination of each species using current DNA barcodes, we performed the fluorescent amplified fragment length polymorphism （F-AFLP） and successfully discriminated each species, subspecies, variety and their cultivar in 74 samples. Then the non-qualitative results obtained from F-AFLP were transformed into two-dimensional barcodes image file of each cultivar via the PDF417 software. This method was also successfully applied to the discrimination of 17 Chinese indigenous pig breeds. The barcode we constructed which greatly reduces the information storage space is genotypes-specific, and can be conveniently decoded into the original data and thereby be conveniently shared and referred to. We believe that it is possible to construct a new data sharing molecular barcode system that could discriminate the subspecies, varieties, cultivars and even individuals with close genetic relationships.

关键词： molecular barcode molecular marker Cruciferae Sus F-AFLP

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Effect of the Antisense BcMF12 Driven by the BcA9 Promoter on Gene Silencing in Brassica campestris ***

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Agricultural Sciences in China 2008年第8期7卷 922-928页

作者： SONG jiang-hua ZHANG Li-xin YU Xiao-lin cao jia-shu The Institute of Vegetable Science Zhejiang University Hangzhou 310029 P.R.China College of Horticulture Anhui Agricultural University Hefei 230036 P.R. China College of Plant Protection Anhui Agricultural University Hefei 230036 P.R. China

The study analyzed the silencing of BcMF12 gene regulated by BcA9 promoter in the transgenic pakchoi and confirmed the effect of antisense BcMF12 gene on the pollen development. A conserved BcMF12 gene fragment was amplified from the cDNA of flower buds in pakchoi （Brassica campestris L. ssp. chinensis, syn. B. rapa L. ssp. chinensis） and was fused to the anther specific BcA9 promoter. The plant antisense expression vector was constructed and then introduced into pakchoi via Agrobacterium-mediated transformation. The transgenic plants were screened by antibiotics and molecular analysis. PCR and Southern blot revealed that the antisense BcMF12-GUS fusion gene regulated by BcA9 promoter was integrated into transgenic plants. Northern blot suggested that the expression of BcMF12 gene was down-regulated significantly. The pollen germination rate of transgenic plants with antisense BcMF12 gene decreased as compared with that of the control plants. The expression of the gene BcMF12 related to the pollen development was inhibited by the antisense BcMF12 driven by BcA9 promoter, which consequently affected the pollen development in pakchoi.

关键词： Brassica campestris L. ssp. chinensis BcMF12 BcA9 promoter antisense RNA gene expression GUSactivity

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Expression of an Antisense BcMF3 Affects Microsporogenesis and Pollen Tube Growth in Arabidopsis

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Agricultural Sciences in China 2006年第5期5卷 339-345页

作者： LIU Le-cheng cao jia-shu YU Xiao-lin XIANG Xun FEI Yong-jun Institute of Vegetable Science Zhejiang University Hangzhou 310029 P.R. China College of Horticulture and Gardening Yangtse University Jingzhou 434025 P.R. China

In an effort to provide some information relevant to the molecular mechanism of genic male sterility in plants, BcMF3 gene that encodes a pectin methylesterase was isolated from the fertile B line of Chinese cabbage-pak-choi （Brassica rapa ssp. chinensis, syn. B. campestris ssp. chinensis）. In the present paper, a 455-bp antisense cDNA fragment of BcMF3 was introduced to binary vector pB1121, and then was mobilized into Agrobacterium tumefaciens strain LBA4404. The A. tumefaciens harboring the BcMF3 antisense fragment was transformed to Arabidopsis thaliana by floral dip. Scanning electronic microscopy examination demonstrated that 47.8% of BcMF3 antisense pollen grains exhibited abnormal shape, which might lead to decreased germination of pollens, suggesting that the product of BcMF3 gene plays an important role during microsporogenesis. The evidence on burst of 45.7% of BcMF3 antisense pollen tubes in vitro and a majority of BcMF3 antisense pollens restricted within the stigmatic tissue revealed that BcMF3 is involved in aiding the growth of pollen tubes. The results suggest that BcMF3 acts at both stages of microsporogensis and pollen tube growth.

关键词： antisense BcMF3 microsporogenesis pectin methylesterase (PME) pollen

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基于生物适应进化原理论证生物进化的动力

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遗传 2010年第8期32卷 791-798页

作者：曹家树浙江大学蔬菜研究所杭州310029

文章从现有主流生物进化理论存在的问题入手,以生物适应进化原理为认识基础,讨论生物进化的动力,以求对生物进化机制有一个新的认识。在薛定谔"生命赖负熵生存"观点的指导下,提出了"负熵流"包括能量流、物质流和信息流,以及负熵流是生... 详细信息

文章从现有主流生物进化理论存在的问题入手,以生物适应进化原理为认识基础,讨论生物进化的动力,以求对生物进化机制有一个新的认识。在薛定谔"生命赖负熵生存"观点的指导下,提出了"负熵流"包括能量流、物质流和信息流,以及负熵流是生命生存和发育的动力的观点。作者在原有生物适应进化原理基础上,修改完善并提出了"DNA、RNA和蛋白质在环境作用下的生物适应进化调控系统"理论,并根据系统发育是个体发育的"积分"的观点,推论得出生物与环境的负熵差引起的负熵流也是生命进化的动力,对生物进化机制作出了新的理解。基于这样的生物进化机制的认识,提出了"进化是一个子系统在其上一等级系统中,将自身全部或部分信息遗传给下一代子系统,并在其适应上一等级系统过程中,产生一些新质,终止一些旧质,从而在其上一等级系统中得以延续的变化过程"的概念,并探讨了一些与进化有关的其他争议问题。

关键词：进化生物进化机制生物进化动力个体发育系统发育生物适应进化

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胞质雄性不育白菜叶绿体ndhJ-trnF区域序列发生变异

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生物化学与生物物理进展 2007年第5期34卷 497-502页

作者：蒋明曹家树浙江大学蔬菜研究所细胞与分子生物学实验室杭州310029

以来自Polima胞质甘蓝型油菜雄性不育源转育获得的不育白菜'Bpol97-05A'和其回交亲本即保持系'Bajh97-01B'为材料,利用cDNA扩增片段长度多态性(cDNA-AFLP)技术获得一条长约330bp的特异片段P1708,RT-PCR验证确认该序列为不育白菜材料所特... 详细信息

以来自Polima胞质甘蓝型油菜雄性不育源转育获得的不育白菜'Bpol97-05A'和其回交亲本即保持系'Bajh97-01B'为材料,利用cDNA扩增片段长度多态性(cDNA-AFLP)技术获得一条长约330bp的特异片段P1708,RT-PCR验证确认该序列为不育白菜材料所特有,经测序和BLAST比对,发现该片段除54bp的插入序列外,其余部分与大白菜和甘蓝叶绿体ndhJ-trnF基因之间的一段序列完全一致.根据基因区域两端的保守部位设计引物,以Polima不育白菜DNA和可育甘蓝型油菜的DNA为模板,分别获得了长约1900bp的序列,比较序列发现:不育白菜与可育白菜、甘蓝型油菜的DNA序列存在一定差异,'Bpol97-05A'中除多个位点发生变异外,另有108bp的插入序列,该插入由2个长度为54bp的重复序列组成,重复序列中除5′端3个碱基CTT外,其余部分均与trnF基因3′端51bp完全相同.

关键词：白菜 cDNA扩增片段长度多态性(cDNA-AFLP) ndhJ-trnF区域序列变异细胞质雄性不育叶绿体基因

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The Method of Polynomial Particular Solutions for Solving Nonlinear Poisson-Type Equations

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Acta Mechanica Solida Sinica 2024年第1期37卷 155-165页

作者： Zhile jia Yanhua cao Xiaoran Wu School of Sciences East China Jiaotong UniversityNanchang 330013China

In this paper,the method of polynomial particular solutions is used to solve nonlinear Poisson-type partial differential equations in one,two,and three *** condition number of the coefficient matrix is reduced through the implementation of multiple scale technique,ultimately yielding a stable numerical *** methodological process can be divided into two main parts:first,identifying the corresponding polynomial particular solutions for the linear differential operator terms in the governing equations,and second,employing these polynomial particular solutions as basis function to iteratively solve the remaining nonlinear terms within the governing ***,we investigate the potential improvement in numerical accuracy for equations with singularities in the analytical solution by shifting the computational domain a certain *** experiments are conducted to assess both the accuracy and stability of the proposed method.A comparison of the obtained results with those produced by other numerical methods demonstrates the accuracy,stability,and efficiency of the proposed method in handling nonlinear Poisson-type partial differential equations.

关键词： Nonlinear equation Singularity Polynomial particular solutions Poisson type

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Plant Male Sterility Induced by Anti-Gene CYP86MF in Brassica oleracea var. italica

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Agricultural Sciences in China 2005年第11期4卷 806-810页

作者： HUANG Ke cao jia-shu YU Xiao-lin YE Wan-zhi LU Gang XIANG Xun Institute of Vegetable Science Zhejiang University Hangzhou 310029 P.R.China Research Center of Vegetable Science Fujian Academy of Agricultural Sciences Fuzhou 350013 P.R.China

An anti-gene CYP86MF was introduced into hypocotyls of broccoli （Brassica oleracea ***. italica Plenck） with Agrobacterium tumefaciens, and the transgenic plants were obtained by kanamycin selection. The results of PCR, Southern blot and Northern blot indicated that the anti-CYP86MF has been integrated into chromosome of the transgenic plant. And also, plants with hypogenetic stamina or ungerminated pollen were observed. The transgenic male sterility plant could fructify via artificial pollination with normal pollen. Thus it was proved that the pistil of male sterility plant was normally developed, and the sterility originated from anti-CYP86MF.

关键词： Brassica oleracea Broccoli Anti-gene Male sterility

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Advances of graphdiyne-supported metal catalysts in thermocatalytic reactions

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Nano Research 2024年第4期17卷 2223-2233页

作者： jia Yu Yida Yang Yuliang Li Changyan cao Weiguo Song Beijing National Laboratory for Molecular Sciences CAS Research/Education Center for Excellence in Molecular SciencesLaboratory of Molecular Nanostructures and NanotechnologyInstitute of ChemistryChinese Academy of SciencesBeijing 100190China School of Chemical Sciences University of Chinese Academy of SciencesBeijing 100049China Beijing National Laboratory for Molecular Sciences CAS Research/Education Center for Excellence in Molecular SciencesLaboratory of organic solidsInstitute of ChemistryChinese Academy of SciencesBeijing 100190China

Supported metal catalysts are widely used in the modern chemical *** electronic interaction between supports and active components is of great significance for heterogeneous ***(GDY),a new type of carbon allotrope with sp-hybridized carbon atoms,πconjugate structure,and electron transmission capability,is a promising candidate as catalyst *** years have witnessed the rapid progress of GDY-supported metal catalysts for different catalysis *** that most processes in the current chemical industry are thermocatalytic reactions,we herein give an overview about the advances and particular characteristics of GDY-supported catalysts in these *** geometric structure and electronic properties of GDY are first ***,the synthesis methods for GDY-supported metal catalysts and their applications in thermocatalytic reactions are discussed,in which the effect of electronic interaction on catalytic performance is ***,the current challenges and future directions of GDY-supported metal catalysts for thermocatalysis are *** is expected that this review will enrich our understanding of the advances of GDY as a superior support for metal catalysts in thermocatalytic reactions.

关键词： graphdiyne metal catalysts thermocatalytic reactions heterogeneous catalysis

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Weakly Polarized Organic Cation-Modified Hydrated Vanadium Oxides for High-Energy Efficiency Aqueous Zinc-Ion Batteries

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Nano-Micro Letters 2024年第7期16卷 169-186页

作者： Xiaoxiao jia Chaofeng Liu Zhi Wang Di Huang Guozhong cao Department of Materials Science and Engineering University of WashingtonSeattleWA 98195USA School of Materials Science and Engineering Tongji UniversityShanghai 201804People’s Republic of China

Vanadium oxides,par-ticularly hydrated forms like V_(2)O_(5)·nH_(2)O(VOH),stand out as promising cathode candidates for aqueous zinc ion batteries due to their adjustable layered structure,unique electronic characteristics,and high theoretical ***,challenges such as vanadium dissolution,sluggish Zn^(2+)diffusion kinetics,and low operating voltage still hinder their direct *** this study,we present a novel vanadium oxide([C_(6)H_(6)N(CH_(3))_(3)]_(1.08)V_(8)O_(20)·0.06H_(2)O,TMPA-VOH),developed by pre-inserting trimethylphenylammonium(TMPA+)cations into *** incorporation of weakly polarized organic cations capitalizes on both ionic pre-intercalation and molecular pre-intercalation effects,resulting in a phase and morphology transition,an expansion of the interlayer distance,extrusion of weakly bonded interlayer water,and a substantial increase in V^(4+)*** modifications synergistically reduce the electrostatic interactions between Zn^(2+)and the V-O lattice,enhancing structural stability and reaction kinetics during *** a result,TMPA-VOH achieves an elevated open circuit voltage and operation voltage,exhibits a large specific capacity(451 mAh g^(-1)at 0.1 A g^(-1))coupled with high energy efficiency(89%),the significantly-reduced battery polarization,and outstanding rate capability and cycling *** concept introduced in this study holds great promise for the development of high-performance oxide-based energy storage materials.

关键词： Zinc-ion battery Vanadium oxide V_(2)O_(5)·nH_(2)O Pre-intercalation Interlayer engineering

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