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Long-term functional maintenance of primary hepatocytes in vitro using macroporous hydrogels engineered through liquid-liquid phase separation

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Nano Research 2024年第3期17卷 1725-1736页

作者： Yang Sun Sheng Yin Jian Cui Zhongxia Wang Yueying Han Ding Ma Shuo Wang Junhua Wu Yi cao Chunping Jiang Xiaosong Gu Jinan Microecological Biomedicine Shandong Laboratory Jinan 250021China Collaborative Innovation Center of Advanced Microstructures National Laboratory of Solid State MicrostructureKey Laboratory of Intelligent Optical Sensing and ManipulationMinistry of EducationDepartment of PhysicsNanjing UniversityNanjing 210093China Department of Hepatobiliary Surgery The Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210093China National Institute of Healthcare Data Science at nanjing university Nanjing UniversityNanjing 210093China

Preserving the functionality of hepatocytes in vitro poses a significant challenge in liver tissue engineering and bioartificial liver,as these cells rapidly lose their metabolic and functional characteristics after *** by the macroporous structures found in native liver tissues,here we develop synthetic hydrogel scaffolds that closely mimic the liver’s structural organization through the phase separation between polyethylene glycol(PEG)and *** hydrogels exhibit interconnected macroporous structures and appropriate mechanical properties,providing an optimal microenvironment conducive to hepatocyte adhesion and the formation of sizable *** to two-dimensional hepatocyte cultures,enhanced functionalities of hepatocytes cultured in our macroporous hydrogels were observed for 14 days,as evidenced by quantitative reverse-transcription–polymerase chain reactions(qRT-PCR),immunofluorescence,and enzyme linked immunosorbent assay(ELISA)*** sequencing data further confirmed the establishment of cell–cell interactions among hepatocytes when cultured in our ***,these hepatocytes maintained a protein expression lineage that closely resembled freshly isolated hepatocytes,particularly in the Notch and tumor necrosis factor(TNF)signaling *** results suggest that the macroporous hydrogels are attractive scaffolds for liver tissue engineering.

关键词： liver tissue engineering phase separation hepatocytes macroporous hydrogel

The Immense Impact of Reverse Edges on Large Hierarchical Networks

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Engineering 2024年第5期36卷 240-249页

作者： Haosen cao Bin-Bin Hu Xiaoyu Mo Duxin Chen Jianxi Gao Ye Yuan Guanrong Chen Tamás Vicsek Xiaohong Guan Hai-Tao Zhang MOE Engineering Research Center of Autonomous Intelligent Unmanned Systems State Key Laboratory of Intelligent Manufacturing Equipment and TechnologySchool of Artificial Intelligence and AutomationHuazhong University of Science and TechnologyWuhan 430074China School of Mechanical and Aerospace Engineering Nanyang Technological UniversitySingapore 639798Singapore Jiangsu Key Laboratory of Networked Collective Intelligence School of MathematicsSoutheast UniversityNanjing 210096China Department of Computer Science Rensselaer Polytechnic InstituteTroyNY 12180USA Department of Electronic Engineering City University of Hong KongHong Kong 999077China Department of Biological Physics Eötvös UniversityBudapest 1117Hungary MOE Key Laboratory for Intelligent Networks and Network Security School of Automation Science and EngineeringXi’an Jiaotong UniversityXi’an 710049China

Hierarchical networks are frequently encountered in animal groups,gene networks,and artificial engineering systems such as multiple robots,unmanned vehicle systems,smart grids,wind farm networks,and so *** structure of a large directed hierarchical network is often strongly influenced by reverse edges from lower-to higher-level nodes,such as lagging birds’howl in a flock or the opinions of lowerlevel individuals feeding back to higher-level ones in a social *** study reveals that,for most large-scale real hierarchical networks,the majority of the reverse edges do not affect the synchronization process of the entire network;the synchronization process is influenced only by a small part of these reverse edges along specific *** surprisingly,a single effective reverse edge can slow down the synchronization of a huge hierarchical network by over 60%.The effect of such edges depends not on the network size but only on the average in-degree of the involved *** overwhelming majority of active reverse edges turn out to have some kind of“bunching”effect on the information flows of hierarchical networks,which slows down synchronization *** finding refines the current understanding of the role of reverse edges in many natural,social,and engineering hierarchical networks,which might be beneficial for precisely tuning the synchronization rhythms of these *** study also proposes an effective way to attack a hierarchical network by adding a malicious reverse edge to it and provides some guidance for protecting a network by screening out the specific small proportion of vulnerable nodes.

关键词： Synchronizability Large hierarchical networks Reverse edges Information flows Complex networks

How topography and neighbor shape the fate of trees in subtropical forest restoration:Environmental filtering and resource competition drive natural regeneration

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Forest Ecosystems 2024年第1期11卷 73-86页

作者： Haonan Zhang Xingshuo Zhang Yingying Lv Yanyan Ni Baokun Xu Xiangnan Han Xiao cao Qingpei Yang Wanggu Xu Zhedong Qian Innovative Research Team for Forest Restoration Mechanisms Nanjing Institute of Environmental SciencesMinistry of Ecology and Environment(MEE)Nanjing 210042China College of Forestry Jiangxi Agricultural UniversityNanchang 330045China

The structure of plant communities at local scales depends on both the spatial heterogeneity of abiotic environmental factors and the biotic interactions within the ***,although environmental filtering due to microtopographic heterogeneity and resource competition among plants caused by spatial variation in tree density and size are considered to be very important in explaining the mechanisms of community assembly,their effects on the processes of individual mortality and recruitment in natural forest regeneration,as well as their relative contributions,are still poorly *** address this,we established a 12-ha permanent plot in a subtropical evergreen broad-leaved forest area and measured microtopographic variables such as elevation,slope,aspect,and terrain position index(TPI)using a total *** monitored the individual mortality and recruitment in forest natural regeneration through repeated surveys at 5-year *** fitted spatial covariance models to jointly use multiple factors from three groups of variables(microtopographic effect,neighborhood density effects,neighborhood size effects)as explanatory variables to analyze their roles in driving the mortality and recruitment of all individual and 12 dominant species in forest natural regeneration at the neighborhood *** results show that:(1)In the crucial early stages of secondary forest restoration,natural regeneration is influenced by a synergy of environmental filtering,due to microtopographic heterogeneity,and resource competition among plants.(2)For distinct species responses,evergreen dominant species'mortality is largely explained by neighborhood effects,while deciduous species are more affected by topographic ***,the adverse effects of larger conspecific trees on younger trees indicate a pattern of competitive pressure leading to mortality among regenerating trees,such pattern emphasis the influence of parent trees on natural regeneration.(3)As trees g

关键词： Natural regeneration Microtopography Neighborhood effects Mortality and recruitment

The source of tropospheric tides

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Earth and Planetary Physics 2020年第5期4卷 449-460页

作者： Xing Li weixing Wan JinBin cao ZhiPeng Ren Key Laboratory of Earth and Planetary Physics Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China School of Space and Environment Beihang UniversityBeijing 100083China Key Laboratory of Space Environment Monitoring and Information Processing Ministry of Industry and Information TechnologyBeijing 100083China Beijing National Observatory of Space Environment Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China Innovation Academy for Earth Science Chinese Academy of SciencesBeijing 100029China university of the Chinese Academy of Sciences Beijing 100049China

With the method of Hough mode decomposition(HMD),the tidal sources of the three main tidal components,namely,the migrating components DW1(diurnal westward propagating wavenumber 1)and SW2(semidiurnal westward propagating wavenumber 2)and the non-migrating component DE3(diurnal eastward propagating wavenumber 3),at the tropospheric altitudes(1–12 km)and in the latitude range of±60°,were obtained from National Centers for Environmental Prediction(NCEP)Climate Forecast System Reanalysis(CFSR)data during the interval from 1988 to *** analyzed these sources in detail at 6 km and obtained the main properties of their yearly *** DW1 source was found to present a weak seasonal variation in the lower latitudes(about±10°–15°).That is,the amplitudes of the DW1 sources were larger in the summer months than in the winter months,and DW1 presented semi-annual variation near the equator(±10°)such that the DW1 source was larger at the equinoxes than at the *** addition,the SW2 source was symmetric and was stronger in the southern hemisphere than in the northern *** SW2 source presented remarkable annual and semi-annual variation such that the amplitudes were largest during the March equinox months and larger during the June solstice *** contrast,DE3 appeared mainly around the equatorial latitudes within about±30°.The DE3 source presented remarkable semiannual variation that was larger around the solstices than the equinoxes in the southern hemisphere,and it was opposite in the northern *** HMD,we found that the tropospheric tides were primarily dominated by some leading propagating Hough modes,specifically,the(1,1),(2,3),and(3,3)modes;the influences of the other Hough modes were *** consequences of an El Niño–Southern Oscillation modulation of tidal amplitudes for the energy and momentum budgets of the troposphere may now be expected to attract *** summary,the above yearly variations of the main tidal sources and

关键词： tropospheric tides Hough mode decomposition yearly variations

Development and Production of HSLA Wear-Resistant Steel

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Journal of Iron and Steel Research International 2011年第S1期18卷 961-965页

作者： cao Yi 1,WANG Zhao-dong 1,WU Di 1,WANG Guo-dong 1,ZHANG Ti 2 (1.State Key Lab.of Rolling and Automation,Northeastern university,Shenyang 110004,Liaoning,China 2.nanjing Iron and Steel Co.,Ltd.,nanjing 210035,Jiangsu,China) Northeastern Univ State Key Lab Rolling & Automat Shenyang 110004 Liaoning Peoples R China nanjing Iron & Steel Co Ltd Nanjing 210035 Jiangsu Peoples R China

In this paper,the development and production of Ti-Cr-B microalloyed NM400 wear-resistant steel were discussed,along with the influence of heat treatment process parameters on the mechanical *** continuous cooling transformation behavior of steel was investigated by means of thermal *** of austenitizing temperature on microstructures and properties of steel were *** laths evolution,carbide transformation and precipitation behavior were observed by *** wear-resistance of steel was compared with other similar *** results show that the steel has high strength and hardness,great low-temperature toughness and *** tensile strength is more than 1300 MPa,and the hardness is in the range of 390-430 *** to the good hardenability,the steel has a uniform hardness distribution on the surface and thickness *** plate shape,cold bending property and weldability of the product can meet the user’s requirement.

关键词： wear-resistant steel heat treatment hardness toughness wear-resistance

Mitophagy in acute central nervous system injuries:regulatory mechanisms and therapeutic potentials

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Neural Regeneration Research 2025年第9期20卷 2437-2453页

作者： Siyi Xu Junqiu Jia Rui Mao Xiang cao Yun Xu Department of Neurology Nanjing Drum Tower HospitalClinical College of Jiangsu UniversityNanjingJiangsu ProvinceChina Department of Neurology Nanjing Drum Tower HospitalChinese Academy of Medical Science&Peking Union Medical CollegeNanjingJiangsu ProvinceChina Department of Neurology Nanjing Drum Tower HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjingJiangsu ProvinceChina State Key Laboratory of Pharmaceutical Biotechnology and Institute of Translational Medicine for Brain Critical Diseases Nanjing UniversityNanjingJiangsu ProvinceChina Jiangsu Key Laboratory for Molecular Medicine Medical School of Nanjing UniversityNanjingJiangsu ProvinceChina nanjing Neurology Medical Center NanjingJiangsu ProvinceChina

Acute central nervous system injuries,including ischemic stro ke,intracerebral hemorrhage,subarachnoid hemorrhage,traumatic brain injury,and spinal co rd injury,are a major global health *** optimal therapies and improving the long-term neurological functions of patients with acute central nervous system injuries are urgent *** are susceptible to damage after acute central nervous system injury,and this leads to the release of toxic levels of reactive oxygen species,which induce cell ***,a selective form of autophagy,is crucial in eliminating redundant or damaged mitochondria during these *** evidence has highlighted the significant role of mitophagy in acute central nervous system *** this review,we provide a comprehensive overview of the process,classification,and related mechanisms of *** also highlight the recent developments in research into the role of mitophagy in various acute central nervous system injuries and drug therapies that regulate *** the final section of this review,we emphasize the potential for treating these disorders by focusing on mitophagy and suggest future research paths in this area.

关键词： autophagy intracerebral hemorrhage ischemic stroke mitochondria mitochondrial biogenesis mitochondrial quality control mitophagy spinal cord injury subarachnoid hemorrhage traumatic brain injury

Mitochondrial rRNA and tRNA and hearing function

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Cell Research 2007年第03期17卷 227-239页

作者： Guangqian Xing~1 Zhibin Chen~1 Xin cao~2 ~1Department of Otolaryngology,the First Affiliated Hospital of nanjing Medical university,nanjing 210029,China ~2Department of Biotechnology,nanjing Medical university,nanjing 210029,China 1Department of Otolaryngology the First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China 1Department of Otolaryngology the First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China 2Department of Biotechnology Nanjing Medical University Nanjing 210029 China

The human ear is a delicate sensory apparatus of hearing for normal communication,and its proper functioning is highlydependent on mitochondrial oxidative *** first mitochondrial point mutation for nonsyndromic andaminoglycoside-induced hearing loss was identified in *** then a number of inherited mitochondrial mutationshave been implicated in hearing *** of the molecular defects responsible for mitochondrial disorder-associatedhearing loss are mutations in the 12S rRNA gene and tRNA *** this review,after a short description of normal hear-ing mechanisms and mitochondrial genetics,we outline the recent advances that have been made in the identification ofdeafness-associated mitochondrial mutations,and discuss how mitochondrial dysfunction contributes to hearing loss.

关键词： mitochondrial DNA rRNA tRNA gene mutation aminoglycosides hearing loss

Reproductive traits associated with invasiveness in Conyza sumatrensis

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Journal of Systematics and Evolution 2009年第3期47卷 245-254页

作者： 1,2Jian-Hua HAO 1Sheng QIANG 1Qian-Qian LIU 1Fei cao 1(Weed Research Laboratory, nanjing agricultural university, nanjing 210095, China) 2(College of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China) (College of Biology and Food Engineering Changshu Institute of Technology Changshu 215500 China)

Conyza sumatrensis (Retz.) E. Walker, a member of Asteraceae, is a highly invasive species. However, its reproduction biology remains poorly known. To understand the role of reproductive traits in successful invasion of the species, we studied several traits of its reproductive system: the miniature capitulum and gynomonoecious sexual system, the biology and phenology of capitula and florets, pollen/ovule ratio, the mating system (self-compatibility), flower visitors, physical traits and dispersal potential of achenes, germination potential of achenes from manually pollinated capitula, and the association of these traits with invasiveness. Our study showed that the reproductive traits of autonomous seed production, versatile mating system of self- and cross-pollination, and generalized pollination system might contribute to the species’ successful invasive capability. The invasiveness was further enhanced by the high and rapid production of achenes, as well as the high percentage, rapid germination rate and high dispersal capability of achenes. It was concluded that in annual or winter-annual weeds, autonomous seed production contributed significantly to the invasiveness of the species.

关键词： Asteraceae Conyza sumatrensis gynomonoecy invasiveness mating system reproduction.

Single-cell RNA-seq reveals the transcriptional program underlying tumor progression and metastasis in neuroblastoma

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Frontiers of Medicine 2024年第4期18卷 690-707页

作者： Zhe Nian Dan Wang Hao Wang Wenxu Liu Zhenyi Ma Jie Yan Yanna cao Jie Li Qiang Zhao Zhe Liu Department of Immunology School of Basic Medical SciencesTianjin Medical UniversityTianjin300070China Zhejiang Key Laboratory of Medical Epigenetics Department of Cell BiologySchool of Basic Medical SciencesHangzhou Normal UniversityHangzhou311121China Department of Pediatric Oncology Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjin’s Clinical Research Center for CancerTianjin300060China Collaborative Innovation Center for Cancer Personalized Medicine Nanjing Medical UniversityNanjing211166China

Neuroblastoma(NB)is one of the most common childhood *** percent of patients present with widely disseminated clinical signs at diagnosis and exhibit poor ***,the molecular mechanisms triggering NB metastasis remain largely *** this study,we generated a transcriptomic atlas of 15447 NB cells from eight NB samples,including paired samples of primary tumors and bone marrow *** used time-resolved analysis to chart the evolutionary trajectory of NB cells from the primary tumor to the metastases in the same patient and identified a common‘starter’subpopulation that initiates tumor development and ***‘starter’population exhibited high expression levels of multiple cell cycle-related genes,indicating the important role of cell cycle upregulation in NB tumor *** addition,our evolutionary trajectory analysis demonstrated the involvement of partial epithelial-to-mesenchymal transition(p-EMT)along the metastatic route from the primary site to the bone *** study provides insights into the program driving NB metastasis and presents a signature of metastasis-initiating cells as an independent prognostic indicator and potential therapeutic target to inhibit the initiation of NB metastasis.

关键词： single-cell RNA sequencing metastasis neuroblastoma epithelial-to-mesenchymal transition