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Effect of high temperature on the expressions of genes encoding starch synthesis enzymes in developing rice endosperms

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Journal of Integrative Agriculture 2015年第4期14卷 642-659页

作者： cao Zhen-zhen PAN Gang WANG Fu-biao WEI Ke-su LI Zhao-wei SHI chun-hai GENG Wei CHENG Fang-min College of Agriculture and Biotechnology Zhejiang University

High temperature is the major environmental factor affecting grain starch properties of cooking rice cultivars. In this study, two non-waxy indica rice genotypes, cv. 9311 and its mutant with extremely high amylose phenotype（9311eha） were used to study the differential expressions of genes in starch synthesis and their responses to high temperature（HT）. Significant increase in apparent amylose content and hot-water-soluble starch content in mutant 9311 eha were genetically caused by a substitution from AGTTATA to AGGTATA at the leader intron 5′ splice site in Wx gene. This mutation resulted in different m RNA transcript levels, m RNA splicing efficiencies and protein levels of Wx between the two rice genotypes, which also lead to the genotype-dependent alteration in the temporal pattern of Wx transcription and granule-bound starch synthase（GBSS） activity in response to HT. However, changes in the activities of other starch synthesizing enzymes and their expressions of distinct isoform genes were not significant with the Wx gene mutation, thus only minor difference in the particle size of starch granule, chain-length distribution and gelatinization enthalpy were found between the two genotypes. The temporal-specific expression of multiple isoform genes responsive to different temperature regiments indicated that the reduction of GBSS transcript expression under HT was generally accompanied by the decreased expressions of SSSIIa, SSSIIIa and SBEIIb. Consequently, high temperature-ripened grains in 9311 eha showed high proportion of intermediate and long B chains and somewhat lower level of short A chain compared to the wildtype. The temperature-dependent alteration of amylose content was not only attributed to the reduced expression of GBSS, but also associated with the complimentary effect of SSSIIa and SBEIIb.

关键词： rice starch synthesis gene expression amylopectin strucrute high temperature

p38 MAPK inhibition alleviates experimental acute pancreatitis in mice

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Hepatobiliary & Pancreatic Diseases International 2015年第1期14卷 101-106页

作者： Ming-Hua cao Jing Xu hai-Dong Cai Zhong-Wei Lv Ya-Jing Feng Kun Li chun-Qiu Chen Yong-Yu Li Department of Pathophysiology Institute of Digestive Disease Tongji University School of Medicine The Seventh People's Hospital of Shanghai The Tenth People's Hospital of Shanghai Tongji University School of Medicine

BACKGROUND： The mitogen-activated protein kinases （MAPKs） signaling pathway is involved in inflammatory process. However,the mechanism is not clear. The present study was to investigate the role of p38 MAPK in acute pancreatitis in ***： Mice were divided into 4 groups： saline control; acute pancreatitis induced with repeated injections of cerulein; control plus p38 MAPK inhibitor SB203580; and acute pancreatitis plus SB203580. The pancreatic histology, pancreatic enzymes, cytokines, myeloperoxidase activity, p38 MAPK and heat shock protein （HSP） 60 and 70 were ***： Repeated injections of cerulein resulted in acute pancreatitis in mice, accompanying with the activation of p38 MAPK and overexpression of HSP60 and HSP70 in the pancreatic tissues. Treatment with SB203580 significantly inhibited the activation of p38 MAPK, and furthermore, inhibited the expression of HSP60 and HSP70 in the pancreas, the inflammatory cytokines in the serum, and myeloperoxidase activity in the ***： The p38 MAPK signaling pathway is involved in the regulation of inflammatory response and the expression of HSP60 and HSP70 in acute pancreatitis.

关键词： acute pancreatitis p38 MAPK heat shock protein

Arabidopsis AtADF1 is Functionally Affected by Mutations on Actin Binding Sites

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Journal of Integrative Plant Biology 2013年第3期55卷 250-261页

作者： chun-hai Dong Wei-Ping Tang Jia-Yao Liu College of Life Sciences Qingdao Agricultural University

The plant actin depolymerizing factor （ADF） binds to both monomeric and filamentous actin, and is directly involved in the depolymerization of actin filaments. To better understand the actin binding sites of the Arabidopsis thaliana L. AtADF1, we generated mutants of AtADF1 and investigated their functions in vitro and in vivo. Analysis of mutants harboring amino acid substitutions revealed that charged residues （Arg98 and Lys100） located at the α-helix 3 and forming an actin binding site together with the N-terminus are essential for both G- and F-actin binding. The basic residues on the β-strand 5 （K82/A） and the α-helix 4 （R135/A, R137/A） form another actin binding site that is important for F-actin binding. Using transient expression of CFP-tagged AtADF1 mutant proteins in onion （Allium cepa） peel epidermal cells and transgenic Arabidopsis thaliana L. plants overexpressing these mutants, we analyzed how these mutant proteins regulate actin organization and affect seedling growth. Our results show that the ADF mutants with a lower affinity for actin filament binding can still be functional, unless the affinity for actin monomers is also affected. The G-actin binding activity of the ADF plays an essential role in actin binding, depolymerization of actin polymers, and therefore in the control of actin organization.

关键词： Actin binding site actin depolymerizing factor Arabidopsis thaliana mutation.

Novel Brфnsted Acidic Ionic Liquid Based on a Cyclic Guanidinium Cation:a Green,Efficient,and Recyclable Dual Slovent-catalyst System for Fisher Esterification

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Chemical Research in Chinese Universities 2007年第6期23卷 665-668页

作者： GUO Xu DUAN hai-feng SUN hai cao Jun-gang LIN Ying-jie Department of Organic Chemistry College of Chemistry Jilin University Changchun 130012 P. R. China

A novel BrФnsted acidic ionic liquid（IL） based on the cyclic guanidinium cation has been synthesized. This IL, as a strong BrФnsted acid catalyst or solvent, shows high catalytic activity and biphsaic behavor in the esterifications of carboxylic acids and alcohols. The produced esters as a separate phase can be conveniently decanted out from the IL and the IL is recyclable without any loss of catalytic activity.

关键词： BrФnsted acid Ionic liquid Guanidinium Fisher esterification

Global Well-posedness of Compressible Bipolar Navier-Stokes-Poisson Equations

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Acta Mathematica Sinica,English Series 2012年第5期28卷 925-940页

作者： Yi Quan LIN Cheng chun HAO hai Liang LI School of Mathematical Sciences Peking University Institute of Mathematics Academy of Mathematics and Systems ScienceHua Loo-Keng Key Laboratory of MathematicsChinese Academy of Sciences Department of Mathematics Capital Normal University

We consider the initial value problem for multi-dimensional bipolar compressible Navier- Stokes-Poisson equations, and show the global existence and uniqueness of the strong solution in hybrid Besov spaces with the in... 详细信息

关键词： Bipolar compressible Navier-Stokes-Poisson system global existence

Equilibrium Crystallization Temperature of Syndiotactic Polystyrene γ Form

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Chinese Journal of Polymer Science 2018年第6期36卷 749-755页

作者： hai Wang chun-Ji Wu Dong-Mei Cui Yong-Feng Men State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied ChemistryChinese Academy of Sciences

The crystallization behavior of syndiotactic polystyrene（s PS） γ form undergoing annealing at various temperatures was investigated using the thermodynamic phase diagram based on Strobl＇s crystallization theory. On the basis of the differential scanning calorimetric results, it was observed that γ form melt-recrystallization occurred at a higher temperature with the increasing lamellar thickness, which resulted from the pre-annealing at the elevating temperature after acetone induced crystallization. Further temperature dependent small-angle X-ray scattering（SAXS） measurement revealed the evolution of the γ form lamellae upon heating until phase transition, involving three different regimes： lamellae stable region（25-90 °C）, melt-recrystallization region（90-185 °C） and pre-phase transition region（185-195 °C）. As a result, recrystallization line, equilibrium recrystallization line and melting line were developed for the s PS γ form crystallization process. Since the melt of γ form involved a γ-to-α/β form phase transition, the melting line was also denoted as the phase transition line in this special case. Therefore, the equilibrium crystallization temperature and melting（phase transition） temperatures were determined at around 390 and 220 °C on the basis of the thermodynamic phase diagram of the s PS γ form.

关键词： Syndiotactic polystyrene γ form Crystallization behavior Equilibrium crystallization temperature Equilibrium melting temperature

Utilizing the dropped packets for data delivery in VANETs

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The Journal of China Universities of Posts and Telecommunications 2013年第3期20卷 48-52页

作者： SONG Chao LIU Ming GONG hai-gang CHEN Gui-hai cao Jian-nong School of Computer Science and Engineering University of Electronic Science and Technology of China School of Computer Science and Engineering Shanghai Jiao Tong University Internet and Mobile Computing Laboratory Department of Computing Hong Kong Polytechnic University

Timely and cost-efficient multi-hop data delivery among vehicles is essential for vehicular ad-hoc networks （VANETs）, and various routing protocols are envisioned for infrastructure-less vehicle-to-vehicle （V2V） communications. Generally, when a packet （or a duplicate） is delivered out of the routing path, it will be dropped. However, we observe that these packets （or duplicates） may also be delivered much faster than the packets delivered along the original routing path. In this paper, we propose a novel tree based routing scheme （TBRS） for ultilizing the dropped packets in VANETs. In TBRS, the packet is delivered along a routing tree with the destination as its root. And when the packet is delivered out its routing tree, it won＇t be dropt immediately and will be delivered for a while if it can arrive at another branch of the tree. We conduct the extensive simulations to evaluate the performance of TBRS based on the road map of a real city collected from Google Earth. The simulation results show that TBRS can outperform the existing protocols, especially when the network resources are limited.

关键词： routing vanet opportunistic forwarding

Numerical analysis of hydraulic fracture propagation in deep shale reservoir with different injection strategies

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第9期16卷 3558-3574页

作者： Yingjie Xia Mingyu Yao Tianjiao Li hai Yang chun'an Tang School of Civil Engineering Dalian University of TechnologyDalian116024China Deep Underground Engineering Research Center Dalian University of TechnologyDalian116024China CNPC Chuanqing Drilling Engineering Company Limited Chengdu610051China

Deep shale reservoirs are characterized by elevated breakdown pressures,diminished fracture complexity,and reduced modified volumes compared to medium and shallow ***,it is urgent to investigate particular injection strategies that can optimize breakdown pressure and fracturing efficiency to address the increasing demands for deep shale reservoir *** this study,the efficiency of various stimulation strategies,including multi-cluster simultaneous fracturing,modified alternating fracturing,alternating shut-in fracturing,and cyclic alternating fracturing,was ***,the sensitivity of factors such as the cycle index,shut-in time,cluster spacing,and horizontal permeability was ***,the flow distribution effect within the wellbore was *** results indicate that relative to multi-cluster simultaneous fracturing,modified alternating fracturing exhibits reduced susceptibility to the stress shadow effect,which results in earlier breakdown,extended hydraulic fracture lengths,and more consistent propagation despite an increase in breakdown *** alternating shut-in fracturing benefits the increase of fracture length,which is closely related to the shut-in ***,cyclic alternating fracturing markedly lowers breakdown pressure and contributes to uniform fracture propagation,in which the cycle count plays an important *** alternating fracturing demonstrates insensitivity to variations in cluster spacing,whereas horizontal permeability is a critical factor affecting fracture *** wellbore effect restrains the accumulation of pressure and flow near the perforation,delaying the initiation of hydraulic *** simulation results can provide valuable numerical insights for optimizing injection strategies for deep shale hydraulic fracturing.

关键词： Fracture propagation Numerical simulation Hydraulic fracturing Rock failure process analysis(RFPA) Injection strategy

Active disturbance rejection control for fractional reaction-diffusion equations with spatially varying diffusivity and time delay

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Science China(Information Sciences) 2022年第2期65卷 254-256页

作者： Rui-Yang CAI Hua-Cheng ZHOU chun-hai KOU College of Information Science and Technology Donghua University School of Mathematics and Statistics Central South University Department of Applied Mathematics Donghua University

Dear editor,Fractional systems have been identified as an excellent tool for describing sub-diffusion phenomena owing to the memory property of the fractional *** all the research fields in the control theory of fractional systems,stabilization occupies an important ***.[1]discussed the boundary stabilization for fractional PDEs (partial differential equations) for the first time,where a feedback controller was designed to stabilize fractional wave equations using numerical methods,

关键词： Boundary control active disturbance rejection control backstepping method fractional Mittag-Leffler stable