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Visual characterization of targeted effect of holo-transferrin-tagged dihydroartemisinin on human breast cancer cells

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Chinese Science Bulletin 2010年第22期55卷 2390-2395页

作者： XIE WeiLing YANG PeiHui ZENG Xin WANG Hui cai HuaiHong cai jiye Department of Chemistry Jinan University Guangzhou 510632 China Key Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Education Institutes Jinan University Guangzhou 510632 China

Targeted drugs could significantly reduce cytotoxic effect and increase therapeutic activity. Dihydroartemisinin (DHA) has been shown to be effective in killing cancer cells. However, it exhibits non-targeted property. Holo-transferrin (TF) is a suitable drug-carrier to target cancer cells, because cancer cells need iron uptake by the TF-mediated mechanism to maintain their uncontrolled growth. Furthermore, TF receptors (TF-R) are highly expressed on cancer cell surfaces. In this paper, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate the different killing effect of 4-(12-Dihydroartemisininoxy) Benzoic Acid Hydrozide-transferrin (DBAH-TF) on human breast cancer cells (MCF-7) cells and human normal breast (HNB) cells, and atomic force microscopy (AFM) was used to visually observe the targeted effect of DBAH-TF on MCF-7 cells. MTT results show that DBAH-TF is 172 times more potent than DHA in killing MCF-7 cells, while the cytotoxic effect of DBAH-TF on HNB cells is merely 1/33 to DHA. Also, the killing effect of DBAH-TF on MCF-7 cells is 286 times that on HNB cells, showing targeted effect. Moreover, there are distinct differences in ultrastructures of cellular surfaces after DBAH-TF and DHA treatment. Through AFM imaging, many characteristic holes were observed on the cancer cell surface after being effected by DBAH-TF, which differ from the holes with irregular shapes affected by DHA. These results visually show that the DBAH-TF targeted drug has more potent killing effect on MCF-7 cells compared with DHA.

关键词：人乳腺癌细胞双氢青蒿素视觉特性全息转铁蛋白受体原子力显微镜转移因子细胞毒作用

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PROBING MOLECULAR INTERACTION BETWEEN TRANSFERRIN AND ANTI-TRANSFERRIN BY ATOMIC FORCE MICROSCOPE

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Chinese Science Bulletin 2006年第4期51卷 405-408页

作者： ZHENG Zhiwen YANG Peihui ZENG Gucheng cai jiye Key Laboratory of Specially Functional Materials and AdvancedManufacturing Technology Ministry of Education South China University of Technology Guangzhou 510640 China Department of Chemistry Jinan University Guangzhou 510632 China

The interaction between transferrin (Tf) and its antibody was investigated by atomic force microscope. Tf-antibody was immobilized on the Au-coated glass slide, and the specific combination between antibody and antigen was also character-ized by AFM. The results showed that holo-transferrin was jogged with anti-transferrin, and binded anti-tran- sferrin more tightly than apo-transferrin. The force- distance curves revealed that the affinity of anti-trans- ferrin and holo-transferrin was much stronger than that of apo-transferrin.

关键词：转铁球蛋白抗转铁球蛋白分子间相互作用原子力显微镜生物大分子

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Differentiation of hepatocytes from mouse embryonic stem cells and its significance

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Hepatobiliary & Pancreatic Diseases International 2005年第2期4卷 291-294页

作者： YunLong Pan jiye cai AnBin Hu Department of General Surgery First Affiliated Hospital of Jinan University Guangzhou 510632 China. tpanyl@***

BACKGROUND:There have been lots of important discoveries in the research on embryonic stem (ES) cells; but few studies have been involving in the differentiation and differentiation ratio of hepatic stem cells. To provide a new source of hepatocytes in hepatocytes transplantation and liver tissue engineering, we investigated the differentiation and differentiation ratio of hepatocytes in the ES cells differentiating system in a certain length of time.;METHODS:BALB/c ES cells from mice were cultured for differentiation in medium without leukemia inhibitory factor (LIF). During the period of differentiation of ES cells, dual immunofluorescence was performed to analyze the expression of hepatic proteins in cytoplasm such as alpha-fetoprotein (AFP) and albumin (ALB). The count of relative hepatocyte-like cells was analyzed by flow cytometry (FCM) and the hepatic differentiation ratio was obtained.;RESULTS:The expression of AFP was first determined at day 7 and that of ALB at day 13. The expressions of AFP and ALB became stronger with the extention of the differentiation time. The hepatic differentiation ratio at day 13 and 21 was 5.5% and 10.4%, respectively.;CONCLUSIONS:ES cells could differentiate into hepatocytes, which should be a new source of hepatocytes in transplantation of hepatocytes and liver engineering.

关键词： hepatocytes embryonic stem cells differentiation differentiation ratio

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Cinobufacini-induced HeLa cell apoptosis enhanced by curcumin

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Chinese Science Bulletin 2013年第21期58卷 2584-2593页

作者： LIU Li JIN Hua OU JinLai JIANG JinHuan PI Jiang KE ChangHong YANG Fen QIAO DongJuan cai HuaiHong cai jiye Department of Chemistry and Institute for Nano-Chemistry Jinan University Department of Pharmaceutics College of Pharmacy Jinan University

When used in combination with certain chemotherapies, curcumin has been shown to increase apoptosis in several cancer cell lines. Here, we report the combined effects of curcumin and cinobufacini on human cervical carcinoma cells. The aim of this study was to examine whether curcumin could enhance apoptosis induced by cinobufacini. 3-(4,5-Dimethylthiazol-2-y1)-2,5- diphenytetrazolium bromide (MTT) assays revealed that the growth and proliferation of HeLa cells could be inhibited by 75% after a combined treatment of 25 μg/mL cinobufacini and 8 μg/mL curcumin. The combined treatment is 3 times more effective than treatment with 25 μg/mL cinobufacini alone. Annexin V-FITC/PI staining, morphological changes and immunofluorescence verified a significant enhancement in cinobufacini-induced apoptosis when cells were also exposed to curcumin. The data showed that the proportion of early apoptotic cells significantly increased from 15.43% in cells treated only with 25 μg/mL cinobufacini to 49.2% in cells treated with 25 μg/mL cinobufacini and 8 μg/mL curcumin. Moreover, compared with treatment of only 25 μg/mL cinobufacini, ROS production increased 1.7-fold, the intracellular free Ca 2+ concentration increased 1.5-fold, and the mitochon- drial membrane potential decreased by 20% in the combined treatment. Simultaneously, the atomic force microscopy (AFM) re- sults suggest that cells treated with a combination of cinobufacini and curcumin varied significantly in shape and ultrastructure. Collapsed cells with leaking cytoplasm, blebbing pores and emerging apoptotic bodies were prevalent. The nanoparticle size increased from 70 nm when the cells were treated with 25 μg/mL cinobufacini to 190 nm when the cells were treated with 25 μg/mL cinobufacini and 8 μg/mL curcumin. The size increase resulted in the cell membrane becoming considerably rough. These results can improve our understanding of combination treatments. Specifically, the combination of cinobufacini and curcumin may

关键词： HeLa细胞细胞凋亡姜黄素诱导原子力显微镜超微结构变化蟾联合治疗

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Numerical Simulations of Snow Accumulation in the Bogie Region of a Train Considering Snow Particle Rotation

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Fluid Dynamics & Materials Processing 2024年第10期20卷 2337-2352页

作者： Hong Lan jiye Zhang Yao Zhang Lu cai State Key Laboratory of Rail Transit Vehicle System Southwest Jiaotong UniversityChengdu610031China College of Railway Transportation Hunan University of TechnologyZhuzhou412000China GD Midea Air-Conditioning Equipment Co. Ltd.Foshan528311China

To investigate the influence of snow particle rotational motion on the accumulation of snow in the bogie region of high-speed trains,an Euler‒Lagrange numerical approach is *** study examines the effects of snow particle diameter and train speed on the ensuing *** is shown that considering snow particle rotational motion causes significant deviation in the particle trajectories with respect to non-rotating *** a deviation increases with larger snow particle diameters and higher train *** snow accumulation on the overall surface of the bogie increases,and the amount of snow on the vibration reduction device varies *** certain conditions,the amount of accumulated snow can increase by several orders of magnitudes.

关键词： High-speed train bogie snow particle rotation discrete phase model snow accumulation

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基于Fluent软件的直喷柴油机螺旋进气道设计

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农业机械学报 2006年第6期37卷 1-4页

作者：蔡忆昔蔡继业江苏大学汽车与交通工程学院

利用Catia进行三维造型、用Fluent仿真模拟作为辅助手段来设计进气道。在分析总结螺旋进气道结构特点的基础上,针对小型柴油机的特点,设计出了引导管较短、较陡的螺旋进气道,建立了该进气管的3-D模型,并借助Fluent软件对该气道内的气流... 详细信息

利用Catia进行三维造型、用Fluent仿真模拟作为辅助手段来设计进气道。在分析总结螺旋进气道结构特点的基础上,针对小型柴油机的特点,设计出了引导管较短、较陡的螺旋进气道,建立了该进气管的3-D模型,并借助Fluent软件对该气道内的气流进行了仿真模拟,得到了对进气道结构改进有价值的结论。

关键词：柴油机螺旋进气道流场模拟

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Detection of Lipopolysaccharide Induced Inflammatory Responses and Anti-inflammatory Effects of Quercetin in RAW264.7 Macrophages Using Atomic Force Microscope

Detection of Lipopolysaccharide Induced Inflammatory Respons...

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第十四届全国中药和天然药物学术研讨会

作者： Jiang Pi jiye cai SMacau University of Science and Technology

＜正＞In recent years,lipopolysaccharide（LPS）activated RAW264.7 cells are widely used as an in vitro inflammatory model for the screen of effective anti-inflammation drugs and the investigation of their exact anti-inflammation *** up to now,there are few data about the effect of LPS on the morphology,especially on the membrane ultrastructure and

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扫描近场光学显微镜及其在单分子探测中的应用

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化学进展 2008年第6期20卷 984-988页

作者：胡明铅蔡继业暨南大学生命科学技术学院化学系广州510632

扫描近场光学显微镜在光学显微镜中具有独特的性能,其突破衍射光限制,具有单分子探测灵敏度,且在研究时不损伤生物样品。文中简要介绍了扫描近场光学显微镜的原理,详述近年来扫描近场光学显微镜在单分子探测中的应用,介绍了扫描近场光... 详细信息

扫描近场光学显微镜在光学显微镜中具有独特的性能,其突破衍射光限制,具有单分子探测灵敏度,且在研究时不损伤生物样品。文中简要介绍了扫描近场光学显微镜的原理,详述近年来扫描近场光学显微镜在单分子探测中的应用,介绍了扫描近场光学显微镜结合量子点对单分子探测的进展,并对单分子探测的前景做了展望。

关键词：扫描近场光学显微镜单分子探测量子点

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An Indirect Immunoassay for Detecting Antigen Based on Fluorescence Resonance Energy Transfer

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American Journal of Analytical Chemistry 2011年第4期2卷 484-490页

作者： Peihui Yang Shuguang Yao Wei Wei jiye cai 不详

An indirect immunoassay for detecting antigen was developed. It was based on fluorescence resonance energy transfer (FRET) and quenching of gold nanoparticles. Bovine serum albumin (BSA) was chosen as model antigen. Fluorescein isothiocyanate (FITC) was attached to anti-BSA antibody (anti-BSA–FITC) as FRET donor, while BSA was conjugated to gold nanoparticles (GNPs–BSA) as FRET acceptor. The formation of anti-BSA–BSA immunocomplex resulted in the FRET between anti-BSA–FITC and GNPs–BSA. Thus, the fluorescence of FRET donor was quenched, and the decreasing fluorescence intensity responded linearly to the concentration of acceptor within the linear range. The concentration of BSA we obtained according to the stoichiometric ratio between BSA and GNPs. Following this approach, we were able to specifically detect BSA. The detection limit for BSA was 0.5 nM and the linear range of the assay was 2.9 - 43.5 nM. It had been successfully applied to specific detection of BSA in serum samples.

关键词： Anti-BSA Antibody BSA Gold Nanoparticles FRET Indirect Immunoassay

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磷脂自组装膜的原子力显微镜研究进展

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生物医学工程学杂志 2008年第2期25卷 472-475页

作者：邓穗平蔡继业暨南大学生物医学工程研究所广州510632

利用磷脂自组装膜在分子水平上研究生物分子的结构和性能,在生物学和仿生学方面有重要研究意义。以原子力显微镜(AFM)为重要的研究工具,综述了磷脂自组装膜的表面形貌、生长动力学的研究进展以及磷脂自组装膜在生物大分子如蛋白质、DNA... 详细信息

利用磷脂自组装膜在分子水平上研究生物分子的结构和性能,在生物学和仿生学方面有重要研究意义。以原子力显微镜(AFM)为重要的研究工具,综述了磷脂自组装膜的表面形貌、生长动力学的研究进展以及磷脂自组装膜在生物大分子如蛋白质、DNA和酶等的高分辨率AFM成像中的应用。

关键词：自组装膜磷脂原子力显微镜高分辨率蛋白质 DNA 酶

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