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The HD-Zip transcription factor GhHB12 represses plant height by regulating the auxin signaling in cotton

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Journal of Integrative Agriculture 2023年第7期22卷 2015-2024页

作者： liu Yan wang Wei-ping ZHANG Lin ZHU Long-fu ZHANG Xian-long HE xin College of Agronomy Hunan Agricultural UniversityChangsha 410128P.R.China national Key laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070P.R.China

Upland cotton(Gossypium hirsutum L.)is the most important natural textile fiber crop *** height(PH)is a significant component of plant architecture,strongly influencing crop cultivation patterns,overall yield,and economic ***,cotton genes regulating plant height have not been fully ***,an HD-Zip gene(GhHB12)was isolated and characterized in cotton,which regulates the abiotic and biotic stress responses and the growth and development *** this study,we showed that GhHB12 was induced by ***,overexpression of GhHB12 induces the expression of HY5,ATH1,and HAT4,represses the spatial-temporal distribution,polar transport,and signaling of auxin,alters the expression of genes involved in cell wall expansion,and restrains the plant height in *** results suggest a role of GhHB12 in regulating cotton plant height,which could be achieved by affecting the auxin signaling and cell wall expansion.

关键词： cotton GhHB12 plant height auxin cell wall HD-Zip

CREBBP/EP300 mutations promoted tumor progression in diffuse large B-cell lymphoma through altering tumorassociated macrophage polarization via FBXW7-N0TCHCCL2/ CSF1 axis

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Signal Transduction and Targeted Therapy 2021年第2期6卷 482-495页

作者： Yao-Hui Huang Kun cai Peng-Peng Xu Li wang Chuan-xin Huang Ying Fang Shu Cheng Xiao Jian Sun Feng liu Jin-Yan Huang Meng-Meng Ji Wei-Li Zhao Shanghai Istitute of Hematology State Key Laboratory of Medical GenomicsNational Research Center for Translational Medicine at ShanghaiRulin Hospital Afliated to Shanghai Jiao Tong University School of MedicineShanghaiChina school of Life sciences and Biotechnology.Shanghai Jiao Tong Universiy ShanghalChina Department of Immunobiology and Microbiology Shanghai Istitute of ImmunologyShanghai Jiao Tong University School of MedicineShanghaiChina Pole de Recherches Sino Francais en science du Vivant et Genomique Laboratory of Molecular PathologyShanghalChina

Epigenetic alterations play an important role in tumor progression of diffuse large B-cell lymphoma(DL BCL).However,the biological relevance of epigenetic gene mutations on tumor microenvironment remains to be *** core set of genes relating to histone methylation(KMT2D,KMT2C,EZH2),histone acetylation(CREBBP,EP300),DNA methylation(TET2),and chromatin remodeling(ARID1A)were detected in the training cohort of 316 patients by whole-genome/exome sequencing(WGS/WES)and in the validation cohort of 303 patients with newly diagnosed DL BCL by targeted *** correlation with peripheral blood immune cells and clinical outcomes were *** mechanisms on tumor microenvironment were investigated both in vitro and in *** all 619 DLBCL patients,somatic mutations in KMT2D(19.5%)were most frequently observed,followed by mutations in ARID1A(8.7%),CREBBP(8.4%),KMT2C(8.2%),TET2(7.8%),EP300(6.8%),and EZH2(2.9%).Among them,CREBBP/EP300 mutations were signifcantly associated with decreased peripheral blood absolute lymphocyte-to-monocyte ratios,as well as inferior progression-free and overall *** B-lymphoma cells,the mutation or knockdown of CREBBP or EP300 inhibited H3K27 acetylation,downregulated FBXW7 expression,activated the NOTCH pathway,and downstream CCL2/CSF1 expression,resulting in tumor-associated macrophage polarization to M2 phenotype and tumor cell *** B-lymphoma murine models,xenografted tumors bearing CREBBP/EP300 mutation presented lower H3K27 acetylation,higher M2 macrophage recruitment,and more rapid tumor growth than those with CREBBP/EP300 wild-type control via FBXW7-NOTCH-CCL2/CSF1 *** work thus contributed to the understanding of aberrant histone acetylation regulation on tumor microenvironment as an alternative mechanism of tumor progression in DLBCL.

关键词： FBXW7 P300 macrophage

Scutellarin protects oxygen/glucose-deprived astrocytes and reduces focal cerebral ischemic injury

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Neural Regeneration Research 2018年第8期13卷 1396-1407页

作者： Jing-Bo Sun Yan Li Ye-Feng cai Yan Huang Shu liu Patrick KK Yeung Min-Zhen Deng Guang-Shun Sun Prince LM Zilundu Qian-Sheng Hu Rui-xin An Li-Hua Zhou Li-xin wang Xiao Cheng Department of Neurology Guangdong Provincial Hospital of Traditional Chinese Medicine Guangzhou Guangdong Province China Department of Second Institute of Clinical Medicine Guangzhou University of Traditional Chinese Medicine Guangzhou Guangdong Province China Guangdong Provincial Academy of Chinese Medical sciences Guangzhou Guangdong Province China Guangdong Provincial Chinese Emergency Key laboratory Guangzhou Guangdong Province China Department of Anatomy An Hui Medical University Hefei Anhui Province China Department of Biomedical sciences The University of Hong Kong Hong Kong Special Administrative Region China Department of Preventive Medicine School of Public Health Zhong Shan School of Medicine Sun Yat-Sen University Guangzhou Guangdong Province China Guangzhou Department of Anatomy Zhong Shan School of Medicine Sun Yat-Sen University Guangzhou Guangdong Province China

Scutellarin, a bioactive flavone isolated from Scutellaria baicalensis, has anti-inflammatory, anti-neurotoxic, anti-apoptotic and anti-oxida- tive effects and has been used to treat cardiovascular and cerebrovascular diseases in china. However, the mechanisms by which scutellarin mediates neuroprotection in cerebral ischemia remain unclear. The interaction between scutellarin and nicotinamide adenine dinucleotide phosphate oxidase 2 （NOX2） was assessed by molecular docking study, which showed that scutellarin selectively binds to NOX2 with high affinity. Cultures of primary astrocytes isolated from the cerebral cortex of neonatal Sprague-Dawley rats were pretreated with 2, 10 or 50 μM scutellarin for 30 minutes. The astrocytes were then subjected to oxygen/glucose deprivation by incubation for 2 hours in glucose-free Dulbecco＇s modified Eagle＇s medium in a 95% N2/5% CO2 incubator, followed by simulated reperfusion for 22 hours. Cell viability was assessed by cell counting kit-8 assay. Expression levels of NOX2, connexin 43 and caspase-3 were assessed by western blot assay. Reactive oxygen species were measured spectrophotometrically. Pretreatment with 10 or 50 μM scutellarin substantially increased viability, reduced the expression of NOX2 and caspase-3, increased the expression of connexin 43, and diminished the levels of reactive oxygen, species in astrocytes subjected to ischemia-＇reperfusion. We also assessed the effects of scutellarin in vivo in the rat transient middle cerebral artery occlusion model of cerebral ischemia-reperfusion injury. Rats were given intraperitoneal injection of 100 mg/kg scutellarin 2 hours before surgery. The Bederson scale was used to assess neurological deficit, and 2,3,5-triphenyltetrazolium chloride staining was used to measure infarct size. Western blot assay was used to assess expression of NOX2 and connexin 43 in brain tissue. Enzyme-linked immunosorbent assay was used to detect 8-hydroxydeoxyguanosine （8-OHdG）, 4-hydroxy-2-no

关键词： nerve regeneration scutellarin cerebral ischemic injury oxygen glucose deprivation/reoxygenation nicotinamide adenine dinucleotide phosphate oxidase 2 reactive oxygen species connexin 43 neural regeneration

SP1 governs primordial folliculogenesis by regulating pregranulosa cell development in mice

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Journal of Molecular Cell Biology 2020年第3期12卷 230-244页

作者： Han cai Bingying liu Huarong wang Guanghong Sun Lizhao Feng Ziqi Chen Jiaqi Zhou Jiawei Zhang Tuo Zhang Meina He Tingting Yang Qirui Guo Zhen Teng Qiliang xin Bo Zhou Hua Zhang Guoliang Xia Chao wang State Key laboratory of Agrobiotechnology College of Biological SciencesChina Agricultural UniversityBeijing 100193China Fujian Provincial Key laboratory of Reproductive Health Research Medical College of Xiamen UniversityXiamen 361102China Key laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western china College of Life SciencesNingxia UniversityYinchuan 750021China

Establishment of the primordial follicle(PF)pool is pivotal for the female reproductive lifespan;however,the mechanism of primordial folliculogenesis is poorly ***,the transcription factor SP1 was shown to be essential for PF formation in *** results showed that SP1 is present in both oocytes and somatic cells during PF formation in the *** of Sp1 expression,especially in pregranulosa cells,significantly suppressed nest breakdown,oocyte apoptosis,and PF formation,suggesting that SP1 expressed by somatic cells functions in the process of primordial *** further demonstrated that SP1 governs the recruitment and maintenance of Forkhead box L2-positive(FOXL2^+)pregranulosa cells using an Lgr5-EGFP-IRES-CreER^T2(Lgr5-KI)reporter mouse model and a FOXL2^+ cell-specific knockdown *** the molecular level,SP1 functioned mainly through manipulation of NOTCH2 expression by binding directly to the promoter of the Notch2 ***,consistent with the critical role of granulosa cells in follicle survival in vitro,massive loss of oocytes in SP1 knockdown ovaries was evidenced before puberty after the ovaries were tranSP1anted under the renal ***,our results reveal that SP1 controls the establishment of the ovarian reserve by regulating pregranulosa cell development in the mammalian ovary.

关键词： SP1 pregranulosa cells primordial follicle formation NOTCH2 premature ovarian insufficiency

Terahertz coherent detection via two-color laser pulses of various frequency ratios

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Chinese Physics B 2019年第9期28卷 391-398页

作者： xin-Yang Gu Ke-Jia wang Zhen-Gang Yang Jin-Song liu wuhan national laboratory for optoelectronics Huazhong University of Science and TechnologyWuhan 430074China school of Optical and Electronic Information Huazhong University of Science and TechnologyWuhan 430074China

The mechanism of terahertz(THz) pulse coherent detection via two-color laser pulses of various frequency ratios in gas plasma is theoretically *** investigations demonstrate that except for the commonly used frequency ratio of 2, other uncommon frequency ratios can also be utilized to detect THz pulse, such as 2 n, n + 1/2(n ≤ 3, n is a positive integer).The well-developed transient photocurrent model is extended to our terahertz detection *** on this model, our simulation results can be explained by analyzing the process of asymmetric electron ionization and electron acceleration.

关键词： terahertz gas plasma coherent detection photoionization

Microstructure and mechanical properties of a low activation cast WTaHfTiZr refractory high-entropy alloy

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china Foundry 2022年第6期19卷 489-494页

作者： Xian-neng Ma Yi-fei Hu Kai wang Hai-long Zhang Zi-tian Fan Jin-ping Suo xin-wang liu State Key laboratory of Materials Processing and Die&Mould technology School of Materials Science and EngineeringHuazhong University of Science and TechnologyWuhan 430074China Dekai Intelligent Casting Co. Ltd.Zhuozhou 072750HebeiChina china Iron and Steel Research Institute Group Beijing 100081China AECC South Industry Company Limited Zhuzhou 412002HunanChina

In the face of the requirement that nuclear fusion reactor materials exhibit more excellent thermal,mechanical and physical properties,a novel refractory highentropy alloy,WTaHfTiZr was *** constituent elements were selected in consideration of low activation,high melting point and high *** alloys were prepared by arc *** as-cast alloy shows a dendrite microstructure with two disordered BCC phases,which caused by the preferential nucleation of W and Ta with much higher melting points during *** exhibits a high compressive yield strength of 1,900 MPa and fracture strain of 8.1% at room temperature,and its yield strengths are up to 612 MPa at 700 ℃ and 203 MPa at 1,000 ℃,*** high strengths are attributed mainly to solid solution strengthening and second phase *** alloy shows great promise as one of the next-generation nuclear fusion reactor materials.

关键词： refractory high-entropy alloy phase structure microstructure mechanical properties

Field evaluation of effects of transgenic cry1Ab/cry1Ac,cry1C and cry2A rice on Cnaphalocrocis medinalis and its arthropod predators

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science china(Life sciences) 2011年第11期54卷 1019-1028页

作者： XU XueLiang HAN Yu WU Gang cai WanLun YUAN BenQi wang Hui liu FangZhou wang ManQun HUA HongXia Hubei Insect Resources Utilization and Sustainable Pest Management Key laboratory College of Plant Science and Technology Huazhong Agricultural University Wuhan 430070 China Institute of Plant Protection Jiangxi Academy of Agricultural Sciences Nanchang 330200 China

The impacts of transgenic Bt rice on target pests and their predators need to be clarified prior to the commercialization of Bt *** this study,the percentages of folded leaves of three transgenic Bt rice lines and non-transgenic parental rice line caused by Cnaphalocrocis medinalis were studied over two successive growing *** addition,the population densities,relative abundance and population dynamics of *** and four species of its natural arthropod predators were investigated at three sites in *** results showed that rice line significantly affected the percentages of folded leaves and population densities of *** *** higher percentages of folded leaves were observed on the non-transgenic rice compared with the three transgenic Bt rice on most sampling *** higher densities of *** larvae and higher relative abundance of *** within phytophages were found on non-transgenic rice compared with three transgenic Bt rice at different sites across the study *** population dynamics of *** larvae were significantly affected by rice line,rice line×sampling date,rice line×year,rice line×sampling date×***,there was little,if any,significant difference in the relative abundance,population density and population dynamics of the four arthropod predators between the three Bt rice lines and non-transgenic *** results of this study indicate that the Bt toxin in transgenic Bt rice can effectively suppress the occurrence of ***,but has no significant effects on the occurrence of the four predatory arthropod species.

关键词： transgenic Bt rice Cnaphalocrocis medinalis population dynamics percentages of folded leaves natural arthropod predator

LncRNA ZFAS1 regulates cardiomyocyte differentiation of human embryonic stem cells

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BIOCELL 2023年第6期47卷 1407-1416页

作者： YANG CAO YINING liu YANG YU XIAOFEI GUO XIUXIU wang WENYA MA HANJING LI ZHONGYU REN xinLU GAO SIJIA LI HAOYU JI HONGYANG CHEN HONG YAN YANAN TIAN xin wang BENZHI cai Department of Pharmacy The Second Affiliated Hospital of Harbin Medical University(Institute of Clinical PharmacyThe University Key Laboratory of Drug ResearchHeilongjiang Higher Education Institutions)Harbin150086China Department of Pharmacology(State-Province Key Laboratories of Biomedicine-Pharmaceutics of china Key Laboratory of Cardiovascular Medicine ResearchMinistry of Education)College of PharmacyHarbin Medical UniversityHarbin150081China

Background:Cardiomyocytes derived from human embryonic stem cells(hESCs)are regulated by complex and stringent gene networks during *** non-coding RNAs(lncRNAs)exert critical epigenetic regulatory functions in multiple differentiation ***,the involvement of lncRNAs in the differentiation of hESCs into cardiomyocytes has not yet been fully ***,we identified the key roles of ZFAS1(lncRNA zinc finger antisense 1)in the differentiation of cardiomyocytes from ***:A model of cardiomyocyte differentiation from stem cells was established using the monolayer differentiation method,and the number of beating hESCs-derived cardiomyocytes was *** expression was analyzed by quantitative real-time PCR(qRTPCR).Immunofluorescence assays were performed to assess the expression of cardiac troponin T(cTnT)andα-actinin protein in ***:qRT-PCR showed that ZFAS1 expression in the mesoderm was significantly higher than that in embryonic stem cells,cardiac progenitor cells,and *** of ZFAS1 inhibited cardiomyocyte differentiation from hESCs,which was characterized by reduced expression of the cardiac-specific markers cTnT,α-actinin,myosin heavy chain 6(MYH6),and myosin heavy chain 7(MYH7).In contrast,ZFAS1 overexpression remarkably increased the percentage of spontaneously beating *** terms of the mechanism,we found that ZFAS1 is an antisense lncRNA at the 5′end of the protein-coding gene *** of ZFAS1 could increase the mRNA expression level of ***,qRT-PCR demonstrated that the silencing of ZNFX1 led to an increase in cardiac-specific markers that predicted the promotion of cardiomyocyte ***:Altogether,these data suggest that lncRNA-ZFAS1 is required for cardiac differentiation by functionally inhibiting the expression of ZNFX1,which may provide a reference for the treatment of heart disease to a certain extent.

关键词： Long non-coding RNAs Cardiomyocyte differentiation ZFAS1 ZNFX1

−22-Fold of^(1)H signal enhancement in-situ low-field liquid NMR using nanodiamond as polarizer of overhauser dynamic nuclear polarization

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Chinese Chemical Letters 2021年第11期32卷 3483-3486页

作者： Zhen Zhang Fang Chen Jiwen Feng Junfei Chen Li Chen Zhi Zhang Huijuan wang xin Cheng Maili liu Chaoyang liu State Key laboratory of Magnetic Resonance and Atomic and Molecular Physics Wuhan Center for Magnetic ResonanceWuhan Institute of Physics and MathematicsInnovation Academy for Precision Measurement Science and TechnologyChinese Academy of SciencesWuhan 430071China university of Chinese Academy of sciences Beijing 100049China wuhan national laboratory for optoelectronics Huazhong University of Science and TechnologyWuhan 430074China

Nanodiamond(ND)polarizer can be used for dynamic nuclear polarization(DNP),owing to unpaired electrons provided by surface ***,^(1)H enhancement via Overhauser DNP(ODNP)using ND in-situ liquid has been found much smaller than traditional ***,we study the surface properties of ND using electron spin resonance(ESR)and Raman methods *** the enhancement of^(1)H ODNP is explored using ND as polarizer with different nanoparticle sizes and concentrations at home-built 0.06 T DNP *** surface of ND with the size of 30 nm is further modification via high temperature air oxidized and the enhancement was *** results show that nanoparticle sizes and Raman peak intensity ratio of sp^(2)/sp^(3)hybridization are approximate negative correlation and positive correlation to enhancement,***,there is no significant enhancement in the oxidation group,and a−22.5-fold^(1)H ODNP enhancement is achieved in-situ liquid at room temperature,which demonstrate the ND can be used as an efficient *** expect ND to play a greater role in biomedical research,especially for multimodal imaging with improving the performance of ND surface.

关键词： ODNP Polarizer Nanoparticle Enhancement Relaxation