检索结果-南通市图书馆

Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope

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Science China Chemistry 2016年第11期59卷 1519-1524页

作者： Shaopeng Wang Suhui Deng xiaoqing cai Shangguo Hou Jiajun Li Zhaoshuai Gao Jiang Li Lihua Wang Chunhai Fan Division of Physical Biology&Bioimaging Center Shanghai Synchrotron Radiation FacilityCAS Key Laboratory of Interfacial Physics and TechnologyShanghai Institute of Applied PhysicsChinese Academy of SciencesShanghai 201800China University of Chinese Academy of Sciences Beijing 100049China School of Information Engineering Nanchang UniversityNanchang 330031China

The significant role of telomeres in cells has attracted much attention since they were *** imaging is an effective method to study subcellular structures like ***,the diffraction limit of traditional optical microscope hampers further investigation on *** progress on superresolution fluorescence microscopy has broken this *** this work,we used stimulated emission depletion(STED) microscope to observe fluorescence-labeled telomeres in interphase cell *** results showed that the size of fluorescent puncta representing telomeres under the STED microscope was much smaller than that under the confocal *** adjacent telomeres were clearly separated via STED imaging,which could hardly be discriminated by confocal microscopy due to the diffraction *** conclude that STED microscope is a more powerful tool that enable us to obtain detailed information about telomeres.

关键词： telomere superresolution imaging stimulated emission depletion(STED) microscopy fluorescence in situ hybridization(FISH)

Sonocatalytic hydrogen/hole-combined therapy for anti-biofilm and infected diabetic wound healing

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National Science Review 2023年第5期10卷 262-271页

作者： Qingqing Xu Shengqiang Chen Lingdong Jiang Chao Xia Lingting Zeng xiaoqing cai Zhaokui Jin Shucun Qin Wenjiang Ding Qianjun He Taishan Institute for Hydrogen Biomedical Research School of Basic Medical SciencesThe Second Affiliated Hospital of Shandong First Medical University&Shandong Academy of Medical Sciences Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging School of Biomedical EngineeringMedical SchoolShenzhen University Shanghai Key Laboratory of Hydrogen Science&Center of Hydrogen Science School of Materials Science and EngineeringShanghai Jiao Tong University

It is a great challenge to effectively eradicate biofilm and cure biofilm-infected diseases because dense extracellular polymeric substance matrix prevents routine antibacterial agents from penetrating into biofilm.H2is an emerging energy-regulating molecule possessing both high biosafety and high tissue *** this work,we propose a concept of sonocatalytic hydrogen/hole-combined ’inside/outside-cooperation’anti-biofilm for promoting bacteria-infected diabetic wound healing based on two-dimensional piezoelectric ***-of-concept experiments using C3N4nanosheets as a representative piezoelectric catalyst with wide band gap and high biosafety have verified that sonocatalytically generated H2and holes rapidly penetrate into biofilm to inhibit bacterial energy metabolism and oxidatively deprive polysaccharides/NADH in biofilm to destroy the bacterial membrane/electron transport chain,respectively,inside/outside-cooperatively eradicating biofilm.A bacteria-infected diabetic wound model is used to confirm the excellent in vivo antibacterial performance of sonocatalytic hydrogen/hole-combined therapy,remarkably improving bacteria-infe cted diabetic wound *** proposed strategy of sonocatalytic hole/hydrogen-combined ’inside/outside-cooperation’ will make a highway for treatment of deep-seated biofilm infection.

关键词： nanocatalytic medicine hydrogen therapy biofilm diabetic foot piezoelectric catalysis

Seasonal Distribution of Observed Colored Dissolved Organic Matter(CDOM)in the Eastern Indian Ocean

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Journal of Ocean University of China 2023年第4期22卷 886-896页

作者： cai xiaoqing MENG Jie YUAN Chao LIU Rongjie HOU Erhu BI Dayong LI Yaoru QIAN Chengcheng ZHONG Shan Shandong Key Laboratory of Marine Ecological Environment and Disaster Prevention and Mitigation Qingdao 266061China North China Sea Marine Forecasting Center Ministry of Natural ResourcesQingdao 266061China National Ocean Technology Center Ministry of Natural ResourcesTianjin 300112China The First Institute of Oceanography Ministry of Natural ResourcesQingdao 266061China North China Sea Environmental Monitoring Center Ministry of Natural ResourcesQingdao 266033China

Colored dissolved organic matter(CDOM)is a crucial constituent that affects the optical absorption properties of *** to the relatively limited measured data on the spatial distribution characteristics of CDOM in the tropical eastern Indian Ocean,this study analyzes the optical absorption characteristics of CDOM in the southeast Indian Ocean using the data collected during four seasons from 2013 to *** work also systematically describes the seasonal horizontal and vertical distribution characteristics of CDOM in this area and conducts a preliminary analysis of the relevant factors affecting CDOM absorption characteristics in this *** indicate that the CDOM ag(440)during summer was remarkably lower than that in the coastal waters of Europe and coastal waters of China but slightly higher than that in the western and southeast *** spatial distribution of surface CDOM shows remarkable seasonal differences,and the spatial distribution characteristics of CDOM in the 5°S,92°E region differ between spring/summer and autumn/*** values of ag(400)and ag(440)are weak/strong at a surface/subsurface level of 100 m,with differences found between summer and *** correlation of CDOM with temperature,salinity,and chlorophyll-a concentration is relatively low,indicating that CDOM is an independent driving mechanism influenced by phytoplankton degradation,photobleaching,and water mixing.

关键词： colored dissolved organic matter tropical eastern Indian Ocean distribution characteristics influencing factors

High expression of GPR160 in prostate cancer is unrelated to CARTp-mediated signaling pathways

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Acta Pharmaceutica Sinica B 2024年第3期14卷 1467-1471页

作者： Chenyu Ye Qingtong Zhou Shi Lin Wensheng Yang xiaoqing cai Yiting Mai Yanyan Chen Dehua Yang Ming-Wei Wang Department of Pharmacology School of Basic Medical SciencesFudan UniversityShanghai 200032China Research Center for Deepsea Bioresources Sanya 572025China Department of Pharmacy Children’s Hospital of Fudan UniversityNational Children’s Medical CenterShanghai 201102China State Key Laboratory of Chemical Biology Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China The National Center for Drug Screening Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China University of Chinese Academy of Sciences Beijing 100049China Department of Chemistry School of Sciencethe University of TokyoTokyo 113-0033Japan School of Pharmacy Hainan Medical UniversityHaikou 571199China

To the Editor:G protein-coupled receptors(GPCRs)are the largest group of membrane proteins with over 800 members,characteristic of a seven transmembrane *** playing crucial roles in regulation of various physiological processes,GPCRs have been implicated in many diseases including diabetes,obesity,depression and *** initiate different intracellular responses,GPCRs mainly interact with three families of effector proteins upon agonist binding:the heterotrimeric G proteins,G protein-coupled receptor kinases(GRKs)and arrestins1.

关键词： GPR160 CARTp Prostate cancer Signaling pathway

DeepCPI:A Deep Learning-based Framework for Large-scale in silico Drug Screening

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Genomics, Proteomics & Bioinformatics 2019年第5期17卷 478-495页

作者： Fangping Wan Yue Zhu Hailin Hu Antao Dai xiaoqing cai Ligong Chen Haipeng Gong Tian Xia Dehua Yang Ming-Wei Wang Jianyang Zeng Institute for Interdisciplinary Information Sciences Tsinghua UniversityBeijing 100084China The National Center for Drug Screening and the CAS Key Laboratory of Receptor Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China School of Medicine Tsinghua UniversityBeijing 100084China School of Pharmaceutical Sciences Tsinghua UniversityBeijing 100084China School of Life Science Tsinghua UniversityBeijing 100084China Department of Electronics and Information Engineering Huazhong University of Science and TechnologyWuhan 430074China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China Shanghai Medical College Fudan UniversityShanghai 200032China MOE Key Laboratory of Bioinformatics Tsinghua UniversityBeijing 100084China

Accurate identification of compound–protein interactions(CPIs)in silico may deepen our understanding of the underlying mechanisms of drug action and thus remarkably facilitate drug discovery and *** similarity-or docking-based computational methods for predicting CPIs rarely exploit latent features from currently available large-scale unlabeled compound and protein data and often limit their usage to relatively small-scale *** the present study,we propose Deep CPI,a novel general and scalable computational framework that combines effective feature embedding(a technique of representation learning)with powerful deep learning methods to accurately predict CPIs at a large *** CPI automatically learns the implicit yet expressive low-dimensional features of compounds and proteins from a massive amount of unlabeled *** of the measured CPIs in large-scale databases,such as Ch EMBL and Binding DB,as well as of the known drug–target interactions from Drug Bank,demonstrated the superior predictive performance of Deep ***,several interactions among smallmolecule compounds and three G protein-coupled receptor targets(glucagon-like peptide-1 receptor,glucagon receptor,and vasoactive intestinal peptide receptor)predicted using Deep CPI were experimentally *** present study suggests that Deep CPI is a useful and powerful tool for drug discovery and *** source code of Deep CPI can be downloaded from https://***/Fangping Wan/Deep CPI.

关键词： Deep learning Machine learning Drug discovery In silico drug screening Compound–protein interaction prediction

Effects of traditional Chinese medicine on treatment outcomes in severe COVID-19 patients:a single-centre study

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Chinese Journal of Natural Medicines 2024年第1期22卷 89-96页

作者： XIAO Yongjiu LI Binbin LIU Chang HUANG Xiuyu MA Ling QIAN Zhirong ZHANG Xiaopeng ZHANG Qian LI Dunqing cai xiaoqing YAN Xiangyong LUO Shuping XIANG Dawei XIAO Kun Emergency Department the 940th Hospital of Jiont Logistics Support Force of Chinese People’s Liberation ArmyLanzhou 730050China Scientific Research Center the Seventh Affiliated HospitalSun Yat-sen UniversityShenzhen 518000China School of Medicine Nankai universityTianjin 300000China Department of Cardiology the 940th Hospital of Joint Logistics Support Force of Chinese People’s Liberation ArmyLanzhou 730050China Department of Radiation Oncology Fujian Medical University Union HospitalFuzhou 350000China Department of Traditional Chinese Medicine the 940th Hospital of Joint Logistics Support Force of Chinese People’s Liberation ArmyLanzhou 730050China Department of Geriatric Diseases the Seventh Medical Center of Chinese PLA General HospitalBeijing 100700China Health and Sport Administration Muskingum UniversityOhioanUSA Laboratory of Vaccine and Antibody Engineering Beijing Institute of BiotechnologyBeijing 100071China Department of Pulmonary and Critical Care Medicine the 940th Hospital of Joint Logistics Support Force of Chinese People’s Liberation ArmyLanzhou 730050China Center of Pulmonary&Critical Care Medicine Chinese People’s Liberation Army(PLA)General HospitalBeijing 100853China

As the search for effective treatments for COVID-19 continues,the high mortality rate among critically ill patients in Intensive Care Units(ICU)presents a profound *** study explores the potential benefits of traditional Chinese medicine(TCM)as a supplementary treatment for severe COVID-19.A total of 110 critically ill COVID-19 patients at the Intensive Care Unit(ICU)of Vulcan Hill Hospital between Feb.,2020,and April,2020(Wuhan,China)participated in this observational *** patients received standard supportive care protocols,with a subset of 81 also receiving TCM as an adjunct *** characteristics during the treatment period and the clinical outcome of each patient were closely monitored and *** findings indicated that the TCM group exhibited a significantly lower mortality rate compared with the non-TCM group(16 of 81 vs 24 of 29;0.3 vs 2.3 person/month).In the adjusted Cox proportional hazards models,TCM treatment was associated with improved survival odds(P<0.001).Furthermore,the analysis also revealed that TCM treatment could partially mitigate inflammatory responses,as evidenced by the reduced levels of proinflammatory cytokines,and contribute to the recovery of multiple organic functions,thereby potentially increasing the survival rate of critically ill COVID-19 patients.

关键词： COVID-19 Pneumonia Novel coronavirus Critically ill Traditional Chinese medicine Clinical outcome

IFLA/OCLC青年研究基金项目与美国图书馆印象

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图书馆建设 2006年第6期 113-115,120页

作者：蔡筱青中山大学图书馆广东广州510275

“IFLA/OCLC青年研究基金项目”旨在为发展中国家的图书馆和信息科学专业人员职业生涯发展及继续教育提供资助,并致力于培养发展中国家的图书馆学和信息学专业的年轻人才。此项目以参观访问活动和主题学习为主线,通过与资深的图书馆员交... 详细信息

“IFLA/OCLC青年研究基金项目”旨在为发展中国家的图书馆和信息科学专业人员职业生涯发展及继续教育提供资助,并致力于培养发展中国家的图书馆学和信息学专业的年轻人才。此项目以参观访问活动和主题学习为主线,通过与资深的图书馆员交流,加深对全球图书馆协作的认识。发达国家图书馆事业发展的成就和经验,对我国多有启示。

关键词： IFLA/OCLC青年研究基金项目国际图书馆协会联合会国际电脑图书馆中心 OCLC委员会年会美国国会图书馆

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中法参考馆员制度比较研究

图书馆学研究（应用版） 2011年第12期 22-25页

作者：蔡筱青中山大学图书馆参考咨询部

法国的参考馆员制度已经有近40年的历史,是欧洲最早实行参考馆员制度的国家之一。对比中法两国图书馆参考馆员制度,法国的参考馆员制度在岗位设置、等级划分、社会地位和职业培训等方面对完善我国图书馆参考馆员制度具有借鉴意义。

关键词：参考馆员制度参考咨询馆员学科馆员参考咨询服务法国国家图书馆比较研究

Structural and functional imaging of brains

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Science China Chemistry 2023年第2期66卷 324-366页

作者： Zhichao Liu Ying Zhu Liming Zhang Weiping Jiang Yawei Liu Qiaowei Tang xiaoqing cai Jiang Li Lihua Wang Changlu Tao Xianzhen Yin Xiaowei Li Shangguo Hou Dawei Jiang Kai Liu Xin Zhou Hongjie Zhang Maili Liu Chunhai Fan Yang Tian Shanghai Key Laboratory of Green Chemistry and Chemical Processes School of Chemistry and Molecular EngineeringEast China Normal UniversityShanghai200241China Interdisciplinary Research Center Shanghai Synchrotron Radiation FacilityZhangjiang LaboratoryShanghai Advanced Research InstituteChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai201210China State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics Chinese Academy of SciencesWuhan National Laboratory for OptoelectronicsWuhan430071China State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun130022China Department of Chemistry Tsinghua UniversityBeijing100084China School of Chemistry and Chemical Engineering Frontiers Science Center for Transformative MoleculesInstitute of Translational MedicineShanghai Jiao Tong UniversityShanghai200240China Interdisciplinary Center for Brain Information Brain Cognition and Brain Disease InstituteShenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research InstitutionsFaculty of Life and Health SciencesShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen518055China Lingang Laboratory Shanghai201602China School of Biomedical Engineering Shanghai Jiao Tong UniversityShanghai200240China Institute of Systems and Physical Biology Shenzhen Bay LaboratoryShenzhen518055China Department of Nuclear Medicine Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China

Analyzing the complex structures and functions of brain is the key issue to understanding the physiological and pathological *** neuronal morphology and local distribution of neurons/blood vessels in the brain have been known,the subcellular structures of cells remain challenging,especially in the live *** addition,the complicated brain functions involve numerous functional molecules,but the concentrations,distributions and interactions of these molecules in the brain are still poorly *** this review,frontier techniques available for multiscale structure imaging from organelles to the whole brain are first overviewed,including magnetic resonance imaging(MRI),computed tomography(CT),positron emission tomography(PET),serial-section electron microscopy(ss EM),light microscopy(LM)and synchrotron-based X-ray microscopy(XRM).Specially,XRM for three-dimensional(3D)imaging of large-scale brain tissue with high resolution and fast imaging speed is ***,the development of elegant methods for acquisition of brain functions from electrical/chemical signals in the brain is *** particular,the new electrophysiology technologies for neural recordings at the single-neuron level and in the brain are also *** also focus on the construction of electrochemical probes based on dual-recognition strategy and surface/interface chemistry for determination of chemical species in the brain with high selectivity and long-term stability,as well as electrochemophysiological microarray for simultaneously recording of electrochemical and electrophysiological signals in the ***,the recent development of brain MRI probes with high contrast-to-noise ratio(CNR)and sensitivity based on hyperpolarized techniques and multi-nuclear chemistry is ***,multiple optical probes and instruments,especially the optophysiological Raman probes and fiber Raman photometry,for imaging and biosensing in live brain are emphas

关键词： brain structure brain function brain chemistry chemical signal biosensing and bioimaging