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Wuhan University Journal of Natural Sciences 2006年第3期11卷 720-724页

作者： LI Zesong cai shaoxi JIANG Yua GUO RuiJun ZHANG Wen College of Bioengineering Chongqing University Chongqing 400044China 不详 Shenzhen Yishengtang Biological Products Co. lad Shenzhen 518054Guangdong China Shenzhen Shajing People＇s Hospital Shenzhen 518104 GuangdongChina

Cultured HSCs were treated colchicine with different concentrations for 12 h, respectively. The effects of eolehicine on HSCs growth were measured by MTT assay. Effects of eolchicine on gene expression of HSCs were analysed by using a self-made oligonucleotide mieroarray. Colehieine inhibited HSCs growth in a dose-dependent manner. After 12 h of treatment with 6.25 mg/L of colehicine, the expression of tissue inhibitor of metalloprotenase-1 （TIMP-1） and the expression of interleukin-6 （IL-6） in HSCs were downregulated by 2.3 folds and 2.1 folds, respectively. These results suggest that colchieine＇s beneficial effects may, at least in part, owe to the inhibitory to the proliferation of HSCs and down regulation of the expression of both TIMP1 and IL-6 in HSCs.

关键词： microarray colchicines tissue inhibitor of metalloprotenase-1 liver fibrosis hepatic stellate cells interleukin-6

PDMS microchannel fabrication technique based on microwire-molding

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Chinese Science Bulletin 2008年第24期53卷 3928-3936页

作者： JIA YueFei JIANG JiaHuan MA XiaoDong LI Yuan HUANG HeMing cai KunBao cai shaoxi WU YunPeng Bioengineering College Chongqing University Chongqing 400044 China Communication Engineering College Chongqing University Chongqing 400044 China

Micro-flow channel is basic functional component of microfluidic chip,and every step-forward of its construction technique has been receiving concern all over the *** article presents a not-complicated but flexible method for fabrication of micro-flow *** method mainly utilizes the conventional molding capability of polydimethylsiloxane(PDMS) and widespread commercial microwires as *** have fabricated out some conventional types of microchannels with different topological shapes,as examples for the demonstration of this flexible fabrication route which was not dependent on the stringent demands of photolithographical or microelectromechanical system(MEMS) *** smooth surface,high-intensity,and high flexibility of the wires made it possible to create many types of topological structures of the two-dimensional or three-dimensional microchannel or channel *** geometric shape of the cross-section of thus forming microchannel in PDMS was the negative of that of embedded-in microwire,in high-fidelity if suitable measures were ***-over,such a microchannel fabrication process can easily integrate the conductivity and low resistivity of the metal wire to create micro-flow devices that are suitable for the electromagnetic control of liquid or the temperature regulation in the *** some preliminary optical analysis was provided for the observation of thus forming rounded *** on this molding strategy,we even made some prototypes for functional microflow application,such as microsolenoids chip and temperature control *** an experiment of forming a droplet in the cross channel further confirmed the feasibility and applicability of this flexible microchannel forming technique.

关键词：微丝软平版印刷术 PDMS 制模方法

A novel in vitro angiogenesis model based on a microfluidic device

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Chinese Science Bulletin 2011年第31期56卷 3301-3309页

作者： DAI XiaoZhen cai shaoxi YE QunFang JIANG JiaHuan YAN XiaoQing XIONG Xin JIANG QiFeng WANG Albert Chih-Lueh TAN Yi Key Laboratory of Biorheological Science and Technology （Chongqing University） of Ministry of Education Bioengineering College Chongqing University Chongqing 400044 China Laboratory Research Center The First Affiliated Hospital of Chongqing Medical University Chongqing 400016 China Muscle and Motility Group Boston Biomedical Research Institute 64 Grove Street Watertown MA 02472 USA Chinese-American Research Institute for Diabetic Complications Wenzhou Medical College Chashan College Park Wenzhou 325035 China Pediatric Diabetes Research at KCHRI of the Department of Pediatrics University of Louisville Louisville KY 40202 USA

Angiogenesis is very important for many physiological and pathological processes. However, the molecular mechanisms of angiogenesis are unclear. To elucidate the molecular mechanisms of angiogenesis and to develop treatments for "angiogenesis-dependent" diseases, it is essential to establish a suitable in vitro angiogenesis model. In this study, we created a novel in vitro angiogenesis model based on a microfluidic device. Our model provides an in vivo-like microenvironment for endothelial cells (ECs) cultures and monitors the response of ECs to changes in their microenvironment in real time. To evaluate the potential of this microfluidic device for researching angiogenesis, the effects of pro-angiogenic factors on ECs proliferation, migration and tube-like structure formation were investigated. Our results showed the proliferation rate of ECs in 3D matrix was significantly promoted by the pro-angiogenic factors (with an increase of 59.12%). With the stimulation of pro-angiogenic factors gradients, ECs directionally migrated into the Matrigel from low concentrations to high concentrations and consequently formed multi-cell chords and tube-like structures. These results suggest that the device can provide a suitable platform for elucidating the mechanisms of angiogenesis and for screening pro-angiogenic or anti-angiogenic drugs for "angiogenesis-dependent" diseases.

关键词：抗血管生成微流体装置模型基体外小说内皮细胞增殖分子机制血管形成

Physiological responses of osteoblasts to cyclic stretching and the change of intracellular calcium concentration

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Chinese Science Bulletin 2003年第4期48卷 346-350页

作者： TANG Liling, WANG Yuanliang, GU Li, cai shaoxi & SU Aihua Bio-engineering College, Chongqing University, Chongqing 400044, China Correspondence should be addressed to Tang Liling Bio-engineering College Chongqing University Chongqing 400044 China

The development of bone tissues is regulated by mechanical stimulation. Cyclic stretching was applied to the osteoblasts that were delivered from rat calvarie. The results showed that stretching at 500 me increased the cell prolifera-tion while loading at 1000 me and 1500 me inhabited cell growth. Loading also increased the adhesive force between cells and substrate as well as spreading areas of osteobalsts. Furthermore, the fluorescence probe Fluo-3/AM was used to investigate the effect of stretching stimulation on the intra-cellular calcium concentration of osteoblasts. The intracellu-lar calcium concentration of osteoblasts that were stretched at 500 me for 5 min was 92.9% higher than the control. After being treated with the panax ontoginseng saponins, the stretched osteoblasts still expressed 28.6% higher intracellu-lar calcium concentration than that of the control, which proved that both the influx of extracellular calcium and the release of intracellular calcium store were involved in the increase of intracellular calcium concentration when os-teoblasts responded to the cyclic stretching. And the influx of extracellular calcium through transmembrance channel played a main role.

关键词：成骨细胞定期拉伸物理性刺激细胞内钙浓度化学刺激生理反应骨质密度

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女性支气管哮喘流行病学与诊治现状

中华结核和呼吸杂志 2019年第3期42卷 173-175页

作者：蔡绍曦赵海金南方医科大学南方医院呼吸与危重症医学科慢性气道疾病实验室广州510515

临床上,支气管哮喘(哮喘)患病率存在明显性别差异。与男性相比,女性哮喘有其自身的特殊性和特点。然而临床对于女性哮喘的危害、发病机制及管理策略尚缺乏系统的认识,还有很多问题尚未阐明。随着哮喘精准治疗不断进步,女性哮喘越来越受... 详细信息

临床上,支气管哮喘(哮喘)患病率存在明显性别差异。与男性相比,女性哮喘有其自身的特殊性和特点。然而临床对于女性哮喘的危害、发病机制及管理策略尚缺乏系统的认识,还有很多问题尚未阐明。随着哮喘精准治疗不断进步,女性哮喘越来越受到临床医生的重视。2018年全球哮喘防治创议(GINA)首次总结围月经期哮喘(perimenstrual asthma,PMA)的特点,强调PMA是哮喘的一种特殊表型,倾向于重症哮喘。本文重点探讨女性哮喘,尤其是PMA的流行病学、发病机制及治疗现状。

关键词：支气管哮喘流行病学女性全球哮喘防治创议诊治临床医生发病机制月经期哮喘

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组织工程中的优化设计

生物医学工程学杂志 2000年第4期17卷 461-463页

作者：潘君王远亮蔡绍皙重庆大学生物工程学院重庆400044

组织工程提供了器官及组织重建的另一种选择 ,是当今的研究热点。本文根据组织工程的组成要素 ,提出优化设计的观点 ,并提出建议 ,为组织工程的实现打下基础。

关键词：组织工程优化设计细胞外基质支撑体信号传导

一种在生物医学工程科研中有实用价值的实验设计方法——均匀设计与正交实验设计联用的实验设计方法

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生物医学工程学杂志 2006年第6期23卷 1228-1231页

作者：唐春红蔡绍皙重庆工商大学环境与生物工程学院药物化学与化学生物学研究中心重庆大学生物工程学院生物力学与组织工程教育部重点实验室重庆400044 重庆大学生物工程学院生物力学与组织工程教育部重点实验室

提出了一种在生物医学工程研究领域中有实用价值的优化实验设计方法。通过比较均匀设计与正交实验设计各自的特点,提出了将两种实验设计方法联合使用的一种新的实验设计方法,对其理论基础和使用方法及特点进行了阐述,并通过实验证明该... 详细信息

提出了一种在生物医学工程研究领域中有实用价值的优化实验设计方法。通过比较均匀设计与正交实验设计各自的特点,提出了将两种实验设计方法联合使用的一种新的实验设计方法,对其理论基础和使用方法及特点进行了阐述,并通过实验证明该方法的有效性。该方法具有均匀设计适用于多因素多水平和实验次数少的特点,回避了均匀设计只能用计算机分析软件才能对数据进行分析的不足,同时保留了正交实验设计应用面广,可以对实验结果进行简单直观地分析的特点,适合于生物医学工程学的科学研究。

关键词：均匀实验设计正交实验设计生物医学工程应用

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病理确诊的组织胞浆菌病:附14例分析

南方医科大学学报 2013年第2期33卷 296-298页

作者：孟莹蔡绍曦李旭南方医科大学南方医院呼吸内科广东广州510515 南方医科大学南方医院急诊科广东广州510515

目的收集南方医科大学南方医院2000-2012年组织胞浆菌病患者的既往史、临床表现、辅助检查结果、诊断、治疗及转归情况,总结分析,以求提高组织胞浆菌病确诊率。方法选择医院2000-2012年经病理活检确诊的14例组织胞浆菌病病例进行回顾性... 详细信息

目的收集南方医科大学南方医院2000-2012年组织胞浆菌病患者的既往史、临床表现、辅助检查结果、诊断、治疗及转归情况,总结分析,以求提高组织胞浆菌病确诊率。方法选择医院2000-2012年经病理活检确诊的14例组织胞浆菌病病例进行回顾性分析。结果组织胞浆菌病临床表现主要为发热、咳嗽、咳痰、胸痛、腹痛等,可合并神经系统症状或出现淋巴结肿大、体表包块。7/14的患者具有基础免疫受抑疾病,9/14出现胸部影像学改变,2/14出现三系减少。确诊前诊断考虑结核、恶性肿瘤、恶性淋巴瘤等疾病,最后均经病理活检确诊。治疗上选择伊曲康唑、两性霉素B、氟康唑及伏立康唑等,治疗后9/10好转。结论组织胞浆菌病发病率低,临床表现多样,影像学特异性不高,极易误诊、漏诊,确诊需靠病原学检查,在诊断后治疗效果不佳时要积极寻找有无潜在疾病,达到确诊目的。

关键词：组织胞浆菌病诊断治疗病理确诊