检索结果-南通市图书馆

comparative study of the transfection efficiency of commonly used viral vectors in rhesus monkey (Macaca mulatta) brains

引用

Zoological Research 2017年第2期38卷 88-95页

作者： Shi-Hao Wu Zhi-Xing Liao joshua D. Rizak Na Zheng Lin-Heng Zhang Hen Tang Xiao-Bin He yang Wu Xia-Ping He Mei-Feng yang Zheng-Hui Li Dong-Dong Qin Xin-Tian Hu Key Laboratory of Animal Models and Human Disease Mechanisms of the chinese Academy of Sciences & Yunnan Province Kunming Institute of Zoology Chinese Academy of Sciences Kunming Yunnan 650223 China Nerve System coding Discipline Group Kunming College of Life Science University of the Chinese Academy of Sciences Kunming Yunnan 650000 China Kunming Primate Research center Kunming Institute of Zoology Chinese Academy of Sciences Kunming Yunnan 650223 China Department of Anatomy and Histology & Embryology Faculty of Basic Medical Science Kunming Medical University Kunming Yunnan 650500 China cAS center for Excellence in Brain Science and Intelligence Technology Chinese Academy of Sciences Shanghai 200031 China center for Excellence in Brain Science Wuhan Institute of Physics and Mathematics Chinese Academy of Sciences Wuhan Hubei 430071 China

Viral vector transfection systems are among the simplest of biological agents with the ability to transfer genes into the central nervous system. In brain research, a series of powerful and novel gene editing technologies are based on these systems. Although many viral vectors are used in rodents, their full application has been limited in non-human primates. To identify viral vectors that can stably and effectively express exogenous genes within non- human primates, eleven commonly used recombinant adeno-associated viral and lentiviral vectors, each carrying a gene to express green or red fluorescence, were injected into the parietal cortex of four rhesus monkeys. The expression of fluorescent cells was used to quantify transfection efficiency. Histological results revealed that recombinant adeno-associated viral vectors, especially the serotype 2/9 coupled with the cytomegalovirus, human synapsin I, or ca2~/calmodulin-dependent protein kinase II promoters, and lentiviral vector coupled with the human ubiquitin c promoter, induced higher expression of fluorescent cells, representing high transfection efficiency. This is the first comparison of transfection efficiencies of different viral vectors carrying different promoters and serotypes in non-human primates （NHPs）. These results can be used as an aid to select optimal vectors to transfer exogenous genes into the central nervous system of non-human primates.

关键词： Recombinant adeno-associated virus Lentivirus Rhesus monkey central nervous system

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Progress on Preparation of Refractory Powder Using Nonmetallic Minerals and Its Applications

引用

china's Refractories 2014年第4期23卷 6-12页

作者： HUANG Zhaohui yang Tao FANG Minghao LIU yangai WU Xiaowen School of Materials Science and Technology Beijing Key Laboratory of Materials Utilization of Nonmetallic Minerals and Solid WastesNational Laboratory of Mineral MaterialsChina University of Geosciences(Beijing)

This review summarized the recent advances in the utilization of nonmetallic minerals and their applications for fabrication of unfired *** preparation of refractory powder utilizing cheap nonmetallic minerals of zircon,low-grade bauxite and quartz was discussed,*** preparation of three unfired Sic-based refractories：Sic-SiAlON-ZrN,Al2O3-Sic/β-SiAlON/Ti（c,N）-c,and Al2O3-Sic-Si3N4 using the as-prepared refractory powders was introduced.

关键词： refractory materials nonmetallic minerals zircon low-grade bauxite quartz unfired refractories

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

在土地使用混合的流域中确定低影响开发(LID)的优先场地

引用

城市规划学刊 2015年第3期 125-126页

作者： MARTIN-MIKLE c j DE BEURS K M jULIAN j P

快速城市化及其相关活动是溪流退化的主要原因之一。随着城市土地面积的增加,流入城区的溪流水质大幅降低。城区的扩张产生了更多的不透水地面,溪流被掩埋,改变了水域水文。低影响开发(LID)是一种以缓解土地开发对环境影响为目标的土地... 详细信息

快速城市化及其相关活动是溪流退化的主要原因之一。随着城市土地面积的增加,流入城区的溪流水质大幅降低。城区的扩张产生了更多的不透水地面,溪流被掩埋,改变了水域水文。低影响开发(LID)是一种以缓解土地开发对环境影响为目标的土地使用总体规划和设计方法,致力于通过(使用雨水桶、屋顶绿化和多孔铺面等)降低本地不透水表面径流,通过(草地洼地、雨水花园和蓄水池)减缓和过滤到达主要溪流的过量径流、泥沙和污染物,通过(保护和恢复岸缓冲区)减慢和过滤邻近主要溪流网络的径流,被认为是减少流入溪流的径流和污染物负荷的有效途径。本文主要介绍了一种空间显性法(spatially-explicit approach),用以

关键词：低影响开发流域雨水花园 LID 集水区尺度水文敏感区土地

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

A NOTE ON THE FORcES OF MORTALITY AND THEIR cLASSIFIcATION IN PEIPING

引用

中华医学杂志(英文版) 1932年第12期 1187-1189页

作者： j.B.GRANT I.c.YUAN Department of Hygiene and Public Health Peiping Union Medical College and First Special Health StationPeiping Municipality.

The development in this country of reporting and of classifying mort-ality has been previously described in detail(1 and 2).National regula-tions exist to provide routines whereby local and provincial authoritiespresumably report mortality statistics to the central *** two official lists of classification of causes of death containing 27and 107 causes respectively(3).The latter is entirely *** should be revised,on the basis of additional *** Peiping health demonstration area has now been establishedfor seven *** registration and classification of deaths has forsix years received as careful consideration as circumstances *** abridged list was adopted in 1926(1)and revised in 1929(2)based

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Assessment of Food Service Quality of chicken Republic and Mega chicken Restaurants, Lagos, Nigeria

引用

Food and Nutrition Sciences 2021年第6期12卷 602-613页

作者： c. j. Akunne P. O. Adeniji Department of Tourism Studies Redeemer’s University Ede Osun State Nigeria

In this project, assessment of food service quality of two restaurants in Lagos was carried out. The study assessed quality of food served in chicken Republic compared with Mega chicken. Three hundred (300) copies of ... 详细信息

In this project, assessment of food service quality of two restaurants in Lagos was carried out. The study assessed quality of food served in Chicken Republic compared with Mega Chicken. Three hundred (300) copies of questionnaires were administered to the customers in Chicken Republic Restaurant while two hundred (200) copies questionnaires were administered to the customer in Mega Chicken Restaurant. In order to analyze the data, simple percentage method was adopted in which the respondents responded to variables like, quality of services, quality of food, healthy food, price and value and condition of environment as factors that determine their patronage. The result of the study showed that the customers prefer Mega Chicken. It also showed that there is a relationship between the customers’ satisfaction and customer patronage in a choice of restaurant. The result of the project could be of use to the intending restaurants’ operators to understand the trend and determinants of customers’ patronage. Recommendation

关键词： Food Quality Food Service Mega chicken customer Service chicken Republic

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A novel hybrid thermodynamic model for pore size distribution characterisation for shale

引用

Petroleum Science 2022年第3期19卷 963-978页

作者： Ze-Zhang Song Abide Abula jun-Yi Zhao Guang-Di Liu Ming-Rui Li Dai-Lin yang Yun-Long Wang State Key Laboratory of Petroleum Resources and Prospecting China University of PetroleumBeijing102249China college of Geosciences China University of PetroleumBeijing102249China The Exploration Department of the changqing Oilfield company PetroChinaXi'anShaanxi710021China Research Institute of Petroleum Exploration and Development Southwest Oil and Gas Field CompanyPetroChinaChengduSichuan 610041China

Scholars often see the gas adsorption technique as a straight-to-interpret technique and adopt the pore size distribution(PSD)given by the gas adsorption technique directly to interpret pore-structure-related *** oversimplification of interpreting shale PSD based on monogeometric thermodynamic models leads to apparent bias to the realistic pore *** work aims at establishing a novel thermodynamic model for shale PSD *** simplified the pore space into two geometric types—cylinder-shaped and ***,Low-temperature Nitrogen Adsorption data were analyzed utilizing two monogeometric models(cylindrical and slit)to generate PSD_(cyl).and PSD_(slit);Secondly,pore geometric segmentation was carried out using Watershed by flooding on typical SEM images to obtain the ratio of slit-shaped(∅_(s))and cylinder-shaped pores(∅_(c)).combining the results of the two,we proposed a novel hybrid *** performed pyrolysis,XRD,FE-SEM observation,quantitative comparison with the results obtained by the DFT model,and fractal analysis to discuss the validity of the obtained PSD_(Hybrid).The results showed that:the hybrid model proposed in this work could better reflect the real geometry of pore space and provide a more realistic PSD;compared with thermodynamic monogeometric models,PSD obtained from the hybrid model are closer to that from the DFT model,with an improvement in the deviation from the DFT model from 5.06%to 68.88%.The proposed hybrid model has essential application prospects for better interpretation of shale pore *** is also worth noting that we suggest applying the proposed hybrid model for PSD analysis in the range of 5-100 nm.

关键词： Pore size distribution Monogeometric thermodynamic model The hybrid model Pore structure Shale

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Family Forest Owners’ Motivation to control Understory Vegetation: Implications for consulting Forestry

引用

Open journal of Forestry 2013年第4期3卷 99-103页

作者： Alex c. Londeau Thomas j. Straka School of Agricultural Forest and Environmental Sciences Clemson University Clemson USA

Forest vegetation management has evolved as a recognized component of intensive forest management practice. It involves the management of competing vegetation necessary to obtain the high yields expected in modern forest plantations via control of interfering plants that influence regeneration outcome, impact timber stand development, and limit native plant and wildlife diversity. It includes cultural control, fire control, mechanical control, biological control, and chemical control. The public perception of forest vegetation management, especially chemical control, is sometimes negative due to health and environmental concerns. It is an important tool in the forest management alternatives available to consulting foresters managing family forest lands (the vast majority of private forest land in the United States). We report on a study that addresses the motivations of family forest owners that implement forest vegetation management practices and the motivation of those who chose not to implement after forester recommendations to do so. For those who do implement forest vegetation management, improvement of wildlife habitat and increased timber growth was the main motivation. For those who did not, cost was the main concern. Size of forest holding plays a major role in determining who will practice intensive forestry.

关键词： Forest Vegetation Management chemical control Family Forest Owners consulting Forestry

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Robust ultrathin and transparent AZO/Ag-SnOx/AZO on polyimide substrate for flexible thin film heater with temperature over 400℃

引用

journal of Materials Science & Technology 2020年第13期47卷 156-162页

作者： Zhaozhao Wang jia Li junjun Xu jinhua Huang Ye yang Ruiqin Tan Guofei chen Xingzhong Fang Yue Zhao Weijie Song School of Materials Science and Engineering Shanghai UniversityShanghai 200444China Ningbo Institute of Materials Technology and Engineering Chinese Academy of SciencesNingbo 315201China Faculty of Information and computer Science Ningbo UniversityNingbo 315211China jiangsu collaborative Innovation center of Photovoltaic Science and Engineering Changzhou 213164China

We developed flexible and transparent thin film heaters(TFHs)with a structure of AZO/AgSnOx/AZO/polyimide(PI)which exhibited superior stability under operational *** and robust doped silver(Ag)films were produced by introducing a small amount of SnOx during the sputtering *** AZO/Ag-SnOx/AZO stacks showed the best figure of merit value of 139.9,which was higher than that of ITO counterpart(25.5)with the same *** addition,it exhibited excellent durability,which showed unaffected optical and electrical properties under the heat treatment of 500℃for 30 min in air,highly-accelerated tempe ratu re and humidity stress test(HAST)at 121℃and 97%RH for 36 h,10,000 times of scratching and 1500 times of inner and outer bending ***,the TFHs based on AZO/Ag-SnOx/AZO/PI achieved a high temperature of 438.8℃with response time in several seconds,which outperformed most previous *** robust high-temperature TFHs in this research hold promising commercial applications in flexible and high temperature occasions.

关键词： Ultrathin Ag films SnOx Doping Durability High temperature thin film heater

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

constructing nanoporous Ni foam current collectors for stable lithium metal anodes

引用

journal of Energy chemistry 2021年第7期30卷 124-132页

作者： Shikun Liu Hongming Zhang Xiaoxu Liu Yu yang caixia chi Shen Wang junying Xue Tingting Hao jiupeng Zhao Yao Li School of chemistry and chemical Engineering Harbin Institute of TechnologyHarbin 150001HeilongjiangChina School of Material Science and Engineering Shaanxi Key Laboratory of Green Preparation and Functionalization for Inorganic MaterialsShaanxi University of Science and TechnologyXi’an 710021ShaanxiChina center for composite Materials and Structure Harbin Institute of TechnologyHarbin 150001HeilongjiangChina

Lithium metal,as the most ideal anode material for high energy density batteries,has been researched for several ***,the dendrite formation and large volume change during repetitive lithium plating/stripping lead to a serious safety issue and impede the practical application of lithium metal ***,a nanoporous Ni foam current collector with high surface area and surface flaws is constructed via a facile oxidation-reduction *** inherent macropore structure of Ni foam can partly accommodate the volume variation during Li plating/*** well-distributed nanopores on the skeleton of Ni foam can effectively reduce the local current density,regulate the uniform lithium nucleation and deposition with homogenous distribution of Li^(+) ***,the surface flaws induce the formation of ring Li structures at initial nucleation/deposition processes and concave Li metal spontaneously formed based on the ring Li structures during cycling,which can direct the even Li plating/***,highly stable coulombic efficiency is achieved at 1 mA cm^(-2) for 200 *** symmetrical cell,based on the nanoporous Ni foam current collector,presents long lifespans of 1200 and 700 h respectively at different current densities of 0.5 and 1 mA cm^(-2) without short *** addition,the LiFePO4 full cell,with the Li metal anode based on the nanoporous Ni foam current collector,shows excellent cycling performance at 1 c for 300 cycles and rate performance.

关键词： Lithium metal anodes Nanopores current collector Ni foam Stable cycling lifespan

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A robust interface enabled by electrospun membrane with optimal resistance in lithium metal batteries

引用

journal of Energy chemistry 2021年第4期30卷 1-9页

作者： chen Dong Zhenkang Lin Yuxin Yin Yaoxuan Qiao Wei Wang Qibing Wu chengxiang yang David Rooney cheng Fan Kening Sun Beijing Key Laboratory for chemical Power Source and Green catalysis School of Chemistry and Chemical EngineeringBeijing Institute of TechnologyBeijing 100081China State Key Laboratory of Advanced chemical Power Sources Guizhou Meiling Power Sources Co.Ltd.Zunyi 563003GuizhouChina School of chemistry and chemical Engineering Queen’s University BelfastBelfast BT95AGUnited Kingdom

A uniform diffusion layer is essential for non-dendritic deposition of lithium in high-density lithium ***,natural pristine solid electrolyte interface(SEI)is always porous and inhomogeneous because of repeated breakdown and repair cycles,whereas ideal materials with excellent mechanical property for artificial SEIs remain a ***,a robust and stable interface is achieved by spinning soft polymer associated with few MoO_(3) into fibers,and thus mechanical property of fibers other than materials determines mechanical performance of the interface which can be optimized by adjusting ***,lithium deposited underneath the layer is enabled by constructing an optimal resistance to make the membrane serve as an artificial SEI rather than lithium *** a result,dendritefree lithium was observed underneath the membrane,and stable interface for long-term cycling was also indicated by EIS *** lithium iron phosphate(LiFePO_(4))full-cell with coated electrode demonstrated an initial capacity of 155.2 m Ah g^(-1),and 80%of its original capacity was retained after 500 cycles at 2.0℃ without any additive in carbonate-based electrolyte.

关键词： Lithium metal battery Dendrite-free Interfacial stability Uniform diffusion layer Deposition site

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：