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Determining the IgG concentrations in bovine colostrum and calf sera with a novel enzymatic assay

Journal of Animal Science and Biotechnology 2019年第1期10卷 239-247页

作者： M.Drikic c.windeyer S.Olsen Y.Fu L.Doepel J.De Buck Department Production Animal Health Faculty of Veterinary MedicineUniversity of Calgary

Background: Immune protection in newborn calves relies on a combination of the timing,volume and quality of colostrum consumed by the calf after birth.Poor quality colostrum with inadequate immunoglobulin concentration contributes to failed transfer of passive immunity in calves,leading to higher calf morbidity and mortality.Therefore,estimating colostrum quality and ensuring the transfer of passive immunity on farm is of critical importance.currently,there are no on-farm tools that directly measure immunoglobulin content in colostrum or serum.The aim of this study was to apply a novel molecular assay,split trehalase immunoglobulin G assay(STIGA),to directly estimate immunoglobulin content in dairy and beef colostrum and calf sera,and to examine its potential to be developed as on-farm test.The STIGA is based on a split version of trehalase TreA,an enzyme that converts trehalose into glucose,enabling the use of a common glucometer for signal detection.In a first study,60 dairy and64 beef colostrum and 83 dairy and 84 beef calf sera samples were tested with STIGA,and the resulting glucose production was measured and compared with radial immunodiffusion,the standard method for measuring immunoglobulin concentrations.Results: Pearson correlation coefficients between the methods were determined and the sensitivity,specificity,and accuracy of the test were calculated for different colostrum quality and failed transfer of passive immunity cut-off points.The correlations of the STIGA measured by colorimetric enzymatic reaction compared to radial immunodiffusion for dairy and beef colostrum were 0.72 and 0.73,respectively,whereas the correlations for dairy and beef sera were 0.9 and 0.85,respectively.Next,STIGA was tested in a blinded study with fresh colostrum and serum samples where the correlation coefficient was 0.93 and 0.94,respectively.Furthermore,the performance of STIGA followed by glucometer readings resulted in correlations with radial immunodiffusion of 0.7 and 0.85 for dairy and beef colostrum and 0.94 and 0

关键词： cattle colostrum Immunoglobulins Passive immunity Radial immunodiffusion Split trehalase

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英国和爱尔兰的人类疱疹病毒-6和-7均可导致神经系统疾病的发病率升高

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世界核心医学期刊文摘（儿科学分册） 2005年第10期1卷 18-19页

作者： Ward K.N. Andrews N.J. Verity c.M. 郭战宏 Department of Infecti on Royal Free and University College Medical School (UCL Campus)Windeyer Insti tute of Medical Sciences 46 Cleveland Street London W1T 4JF United Kingdom Dr.

Background: Primary human herpesvirus-6 and -7 (HHV-6/-7) infections cause febrile illness sometimes complicated by convulsions and rarely encephalopathy. Aims: To explore the extent of such HHV-6 and -7 induced disease in young chi ldren. Methods: In a three year prospective study in Britain and Ireland, 205 ch ildren (2-35 months old) hospitalised with suspected encephalitis and/or severe illness with fever and convulsions were reported via the British Paediatric Sur veillance Unit network. Blood samples were tested for primary HHV-6 and -7 inf ections. Results: 26/156 (17%) of children aged 2-23 months had primary infect ion (11 HHV-6; 13 HHV-7; two with both viruses) coinciding with the acute illness; this was much higher than the about three cases expected by cha nce. All 26 were pyrexial; 25 had convulsions (18 status epilepticus), 11 requir ing ventilation. Median hospital stay was 7.5 days. For HHV-6 primary infection the median age was 53 weeks (range 42-94) and the distribution differed from t hat of uninfected children; for HHV-7, the median was 60 weeks (range 17-102) and the distribution did not differ for the uninfected. Fewer (5/15)-children w ith primary HHV-7 infection had previously been infected with HHV-6 than expec ted. conclusions: Primary HHV-6 and HHV-7 infections accounted for a significa nt proportion of cases in thoseco nvulsions requiring hospital admission; each virus contributed equally. Predispo sing factors are age for HHV-6 and no previous infection with HHV-6 for HHV-7 . children with such neurological disease should be investigated for primary HHV -6/-7 infections, especially in rare cases coinciding by chance with immunisat ion to exclude misdiagnosis as vaccine reactions.

关键词：神经系统疾病人类疱疹病毒癫痫持续状态年龄中位数原发感染发热性疾病罕见病例急性疾病脑病原发性感染

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