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The 4th International conference on continuous casting of Steel in Developing countries

作者： A.Flick c.stoiber K.Mrwald O.Schulz J.Penn c.chimani M.Stiftinger J.Fuchshuber F.Leingruber Siemens VAI Metals Technologies GmbH Linz/Austria

Today,continuous casting is a highly matured *** though new technical and metallurgical developments lead to sustainable improvements in operation,product quality and machine reliability,the increasing requirements of steel plant operation and product end users demand new solutions,thus resulting in completely new options for investors and plant *** adaptation of the casting dimensions through consequent mechatronization of the machine components leads to increases in operation *** approaches in product cooling result in increased production rates and *** solutions provide a quantum leap in process stability and operational *** in machine design and automation allow for the customised application of configurations for new plants and modernisation projects,together with improved serviceability of the *** examples are presented which enable casting machines to produce a wide range of products,such as thin slab casters with ultra high speed casting for close coupled rolling in a HSM,conventional high production and high performance machines and special casters for high alloy content slabs or ultra large casting thickness up to 400 mm. Operational and metallurgical results complete the paper.

关键词： ultra thick slabs ultra large beam blanks high production mechatronics soft reduction robotics

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Activation of mitogen activated protein kinases via complement receptor type 2

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chinese Medical Journal 2004年第12期17卷 1802-1808页

作者：罗敏华陈明亮 Heribert stoiber Manfred P Dierich Institut für Hygiene Innsbruck Universitt Fritz-Pregl-Str.β A 6010 Innsbruck Austria Laboratory of Virology Xiangya School of Medicine Central South University Changsha 410078 China Department of Dermatology Xiangya Hospital Xiangya School of Medicine Central South University Changsha 410008 China

Background complement receptor type 2 (cR2) is the receptor for c3d and c3dg and for Epstein Barr virus The aim of our study was to explore whether cR2 can independently mediate the activation of mitogen activated protein kinases (MAPKs, including ERK, JNK, and p38MAPK), and to highlight the molecular mechanism of cD 4 + cell deletion in AIDS Methods HOS cells (HOS cR2) and HOS cD4 cells (HOS cD4cR2) stably expressing cR2 were established and then identified by FAcS and Western blotting Activation and blocking tests of MAPKs were assessed by Western blot cell proliferation was determined using cell Titer 96  Aqueous One Solution Reagent Results FAcS results showed that the positive rates of HOS cR2 and HOS cD4cR2 cells were greater than 96%, and Western blot showed that the cR2 expression levels on HOS cR2 and HOS cD4cR2 cells were high Activation and blocking tests of MAPKs (ERK, JNK, and p38MAPK) were carried out in HOS cR2, HOS cD4, and HOS cD4cR2 cells The activation of MAPKs in HOS cR2 cells stimulated with PMA (100 ng/ml) and NHS (10%) was identical The activation of MAPKs increased at 5 minutes, reached a peak at 10 minutes, and decreased to baseline within 30 minutes, all in a time dependent manner; the activation of MAPKs was blocked by anti cR2 McAb, PD98059 (inhibitor of ERK), and Wortmanin (inhibitor of PI 3K), respectively In HOS cD4 cells, MAPKs were activated by HIV gp160 In HOS cD4cR2 cells, MAPK activation was induced by HIV gp160, 10% NHS, and HIV gp160+10%NHS; phosphorylation of p38MAPK was dramatically induced by HIV gp160+NHS, and lasted for 1 hour The cell proliferation results showed that HIV gp160 inhibited the proliferation of HOS cD4 and HOS cD4cR2 cells ( P ced the effect of HIV gp160 ( P conclusions The activation of MAPKs is independently mediated by cR2 and that anti cR2 McAb, PD98059, and Wortmanin block the activation of MAPKs, respectively The results of the signal transduction and cell proliferation

关键词： complement receptor type 2 · signal transduction · mitogen activated protein kinases · human immunodeficiency virus

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Activation of mitogen activated protein kinases via complement receptor type 2

Activation of mitogen activated protein kinases via compleme...

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全国新出现传染病学术研讨会

作者：罗敏华 Heribert.stoiber Manfried.P.Dierich Institut fur Hygiene Innsbruck UniversitatFritz-Pregl-Str.3A-6010 InnsbruckAustria

Background complement receptor type 2 (cR2) is the receptor of c3d and c3dg, andEpstein-Barr virus. The aim of our study is to explore if cR2 can mediate the activation of mitogen-activated protein kinases (MAPKs, include ERK, JNK and p38MAPK) independently, and to highlight the molecular mechanism of cD4+ cell deletion in AIDS. Methods cR2 stably expressing HOS cell (HOScR2) and HOS-cD4 cell (HOS-cD4cR2) were established as previously and identified by FAcS and Western Blotting. The activating and blocking test of MAPKs were determined by Western Blotting. cell proliferation was detected by using cell Titer 96 Aqueous One Solution Reagent.

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经cR2激活MAPKs与抑制HIV-gp160处理的cD4^+细胞增殖

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中华微生物学和免疫学杂志 2004年第4期24卷 304-310页

作者：罗敏华李太存陈明亮 H.stoiber M.P.Dierich 苏先狮 Institute fǖr hygiene 中南大学湘雅医学院中南大学湘雅一医院皮肤科中南大学湘雅二医院传染科

目的　建立cR2稳定表达的HOS cR2、HOS cD4cR2细胞系 ,研究细胞中MAPKs的活化和细胞增殖变化 ,阐明经cR2的信号传导、cR2的表达对HIV gp16 0等所致的cD4 +细胞的影响。方法　用哺乳动物细胞稳定转染法建立稳定表达cR2的HOS cR2、HOS cD4... 详细信息

目的　建立cR2稳定表达的HOS cR2、HOS cD4cR2细胞系 ,研究细胞中MAPKs的活化和细胞增殖变化 ,阐明经cR2的信号传导、cR2的表达对HIV gp16 0等所致的cD4 +细胞的影响。方法　用哺乳动物细胞稳定转染法建立稳定表达cR2的HOS cR2、HOS cD4cR2细胞系 ,细胞经磁珠阳性纯化后 ,用FAcS和Westernblot对cR2的表达进行鉴定。用PMA、10 %NHS、HIV gp16 0等处理细胞后 ,经Westernblot检测细胞的MAPKs的活化水平 ;用cellTiter 96AqueousOneSolutionReagent检测和分析处理后细胞的增殖差异。结果　FAcS检测和统计学方法分析结果表明cR2表达的阳性率高达96 %以上 ;Westernblot结果显示所建HOS cR2、HOS cD4cR2细胞系的cR2的表达水平与Raji细胞相近 ;细胞经PMA、预先激活补体的 10 %NHS处理细胞 ,发现在处理 10min时ERK、JNK、P38的活化达高峰 ;PD980 5 9、Wortmanin和抗 cR2能阻断ERK、JNK、P38的活化。HIV gp16 0、预先激活补体的 10 %NHS能相应激活HOS cR2 ,HOS cD4和HOS cD4cR2细胞ERK、JNK、P38,这些激活均可被相应的抗体所阻断 ;细胞增殖实验结果显示HIV gp16 0能抑制细胞增殖 (P cR2的表达能加重HIV gp16 0所致的细胞增殖抑制 (P <0 .0 1)。结论　磁珠阳性纯化法可浓集稳定表达靶基因的细胞 ,提高阳?

关键词：补体受体信号传导蛋白激酶 HIV cD4^+细胞细胞增殖

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火山和地热源流出的全球Hg流量

地震地质译丛

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地震地质译丛 1988年第6期 37-40页

作者： Johaw c.Varekamp Peter R.Buseck 远声

关自然界的Hg流量是很难定的。尽管火山的Hg流量的大小更加难定,但近来的学术会议上有人认为地中海地区大多数的Hg是火山成因的（New Scientist,1984）。cadle（1980）等曾对火山流出的Hg量作过估计,其值约为3—900Mg/年。Siegel根据观... 详细信息

关自然界的Hg流量是很难定的。尽管火山的Hg流量的大小更加难定,但近来的学术会议上有人认为地中海地区大多数的Hg是火山成因的（New Scientist,1984）。cadle（1980）等曾对火山流出的Hg量作过估计,其值约为3—900Mg/年。Siegel根据观测到的Hg/SO2比值提出活动的夏威夷火山的Hg流量。本文中我们给出若干火山的Hg数据,还估算了来自地热源的Hg量。普通评价火山Hg流量的方法是利用观测到的Hg/SO2量并结合全球火山SO2流量的估算值（stoiber,1983）。

关键词：火山丰度去气 Hg 地热热源

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