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Agronomic evaluation of Herbaspirillum seropedicae strain ZAE94 as an inoculant to improve maize yield in Brazil

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Pedosphere 2021年第4期31卷 583-595页

作者： Gabriela c.ALVES carlos L.R.DOS SANTOS Jerri E.ZILLI Fabio B.DOS reis JUNIOR Ivanildo E.MARRIEL Farley A.da F.BREDA Robert M.BODDEY Veronica M.reis Federal Rural University of Rio de Janeiro Department of Soilskm 7 BR 465Seropédica RJ 23900-000(Brazil) Federal University of Mato Grosso 2367 Fernando Corrêa Costa AvenueBoa Esperança M 78060-900(Brazil) Embrapa Agrobiology km 07 BR 465Seropédica RJ 23891-000(Brazil) Embrapa cerrados BR-020 km 18Planaltina DF 73310-970(Brazil) Embrapa Maize and Sorghum MG 424 km 45Sete Lagoas MG 35701-970(Brazil)

Diazotrophic bacteria applied as a seed inoculant can improve the grain yield of several crops including maize. The current study aimed to test the agronomic efficiency and contribution of biological nitrogen fixation(BNF) of the endophytic diazotroph Herbaspirillum seropedicae strain ZAE94 to maize under field conditions. Eighteen field assays were conducted in four different locations during consecutive years on two hybrids and two varieties of maize in a random block design with four replicates using a peat-based inoculant. The inoculant containing the ZAE94 strain was applied without nitrogen(N)fertilization or with 40 kg N ha^(-1) and was compared to the application of 40 and 80 kg N ha^(-1) without inoculation. crop productivity and N accumulation in the grain were evaluated in addition to ^(15)N natural abundance(δ^(15)N) to evaluate BNF in the treatments without N fertilization. Fertilization at 40 kg N ha^(-1) plus bacterial inoculation produced crop yields similar to the treatment with 80 kg N ha^(-1) and increased grain N content, especially in the off-season with 40 kg N ha^(-1). The inoculation treatments showed lower δ^(15)N values than the non-inoculated treatments, which was most evident in the off-season. The BNF contributed about 30% of N accumulated in plants inoculated with ZAE94. On average, 64% of the N fertilized plots showed an increase of the parameters evaluated in the inoculated treatments, compared with the control. Inoculation also increased root length, root volume, and leaf area, and these parameters were positively correlated with plant weight using a hydroponic assay. This study revealed that the application of H. seropedicae inoculant increased the amount of N in plants owing to BNF, and there is a better chance of yield response to inoculation under low N fertilizer application in the off-season.

关键词： agronomic efficiency biological nitrogen fixation endophytic diazotroph inoculation 15N natural abundance plant growth-promoting bacteria

Promoting the production of challenging proteins via induced expression in cHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α

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Synthetic and Systems Biotechnology 2024年第3期9卷 416-424页

作者： Jeffrey L.Schloßhauer Lena Tholen Alexander Korner Stefan Kubick Sofia chatzopoulou Anja Honow Anne Zemella Fraunhofer Project Group PZ-Syn of the Fraunhofer Institute for cell Therapy and Immunology-IZI Branch Bioanalytics and Bioprocesses-IZI-BBAm MühlenbergPotsdamGermany Fraunhofer Institute for cell Therapy and Immunology-IZI Branch Bioanalytics and Bioprocesses-IZI-BBAm MühlenbergPotsdamGermany Faculty of Health Sciences Joint Faculty of the Brandenburg University of Technology Cottbus-SenftenbergThe Brandenburg Medical School Theodor FontaneUniversity of PotsdamPotsdamGermany Institute for chemistry and Biochemistry Laboratory of Protein BiochemistryFreie Universitat BerlinThielallee 6314195BerlinGermany Institute of Biotechnology Technische Universit¨at BerlinStraße des 17.Juni 13510623BerlinGermany New/era/mabs GmbH August-Bebel-Str.8914482PotsdamGermany

chinese hamster ovary(cHO)cells are crucial in biopharmaceutical production due to their scalability and capacity for human-like post-translational ***,toxic proteins and membrane proteins are often difficult-to-express in living ***,cell-free protein synthesis can be *** study explores innovative strategies for enhancing the production of challenging proteins through the modification of cHO cells by investigating both,cell-based and cell-free approaches.A major result in our study involves the integration of a mutant eIF2 translation initiation factor and T7 RNA polymerase into cHO cell lysates for cell-free protein *** resulted in elevated yields,while eliminating the necessity for exogenous additions during cell-free production,thereby substantially enhancing ***,we explore the potential of the Rosa26 genomic site for the integration of T7 RNA polymerase and cell-based tetracycline-controlled protein *** findings provide promising advancements in bioproduction technologies,offering flexibility to switch between cell-free and cell-based protein production as needed.

关键词： Inducible expression cHO cells cell-free protein synthesis cRISPR T7 RNA polymerase eIF2 Rosa26

Enabling affordances of blockchain in agri-food supply chains:A value-driver framework using Q-methodology

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International Journal of Innovation Studies 2023年第4期7卷 325-343页

作者： Pouyan Jahanbin Stephen c.Wingreen Ravishankar Sharma Behrang Ijadi Marlon M.reis college of Business University of CanterburyChristchurch8140New Zealand college of Technological Innovation Zayed UniversityAbu DhabiUnited Arab Emirates AgResearch Ltd Lincoln7674New Zealand

The application of blockchain beyond cryptocurrencies has received increasing attention from industry and scholars *** predicted looming food crises,some of the most impactful deployments of blockchains are likely to concern food supply *** study outlined how blockchain adoption can result in positive affordances in the food supply *** Q-methodology,this study explored the current status of the agri-food supply chain and how blockchain technology could be useful in addressing existing *** theorization leads to the proposition of the 3TIc value-driver framework for determining the enabling affordances of blockchain that would increase shared value for ***,we propose a framework based on the most promising features of blockchain technology to overcome current challenges in the agri-food *** value-driver framework is driven by the Q-study findings of respondents closely associated with the agri-food supply *** framework can provide supply chain stakeholders with a clear perception of blockchain affordances and serve as a guideline for utilizing appropriate features of technology that match organizations’capabilities,core competencies,goals,and ***,it could assist top-level decision-makers in systematically evaluating parts of the organization to focus on and improve the infrastructure for successful blockchain implementation along the agri-food supply *** conclude by noting certain significant challenges that must be carefully addressed to successfully adopt blockchain technology.

关键词： Blockchain technology Agricultural food supply chain Q-methodology Trustworthy platform

Agreement between IOLMaster 500 and Pentacam HR for keratometry assessment in type 2 diabetic and nondiabetic patients

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International Journal of Ophthalmology(English edition) 2020年第6期13卷 920-926页

作者： Joao N Beato Joao Esteves-LeANDro David reis Rita Matos Manuel Falcao Vítor Rosas Angela carneiro FernANDo Falcao-reis Department of Ophthalmology Sao Joao HospitalPorto 4200-319.Portugal Department of Surgery and Physiology Faculty of MedicineUniversity of PortoPorto 4200-319Portugal Faculty of Medicine University of PortoPorto 4200-319Portugal

AIM:To evaluate inter-device agreement of anterior keratometry obtained by the IOLMaster 500 and Pentacam HR in type 2 diabetic and non-diabetic ***:corneal measurements were sequentially performed in 60 diabetes mellitus(DM)and 48 age and sex-matched controls undergoing cataract *** recorded included flat and steep keratometry,mean keratometry(Km),astigmatism magnitude,axis location,JO and J45 ***-Altman plots and intraclass correlation coefficients were used for examination of *** analyses were performed for astigmatism magnitude,diabetes duration,hemoglobin A1c(HbA1c)levels and diabetic retinopathy(DR)***:Agreement for Km and astigmatism magnitude were considered good and moderate,with 95%limits of agreement(LoA)of-1.09 to 1.23 diopters(D)and-0.83 to 0.86 D in DM group,respectively;and-0.59 to 0.72 D and-0.98 to 0.75 D in non-DM group,*** contrast,the 95%LoA for corneal axis exceeded the clinically relevant margins in both *** the total sample,only 41 eyes(38%)had a smaller than 5-degree *** duration,HbA1c levels and DR stage were not found to significantly affect *** regression showed that higher corneal power(P=0.021)and astigmatism magnitude(P=0.011)were associated with a decreased risk of having a difference in axis location greater than ***:In both groups,IOLMaster and Pentacam agree well for corneal power and moderately for ***,axis location disagreement is frequent in eyes with flatter corneas and small amounts of astigmatism.

关键词： keratometry diabetes mellitus astigmatism cataract diabetic retinopathy

Automatic method for the estimation of li-ion degradation test sample sizesrequired to understand cell-to-cell variability

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Energy and AI 2022年第3期9卷 139-147页

作者： calum Strange Michael Allerhand Philipp Dechent Gonçalo dos reis School of Mathematics University of EdinburghThe Kings buildingsEdinburghEH93JFScotlandUnited Kingdom Institute for Power Electronics and Electrical Drives(ISEA) RWTH Aachen UniversityAachenGermany centro de Matematica e Aplicacoes(cMA) Faculdade de Ciencias e TecnologiaCampus da CaparicaCaparica2829-516Portugal

The testing of battery cells is a long and expensive process, and hence understanding how large a test set needsto be is very useful. This work proposes an automated methodology to estimate the smallest sample size ofcells required to capture the cell-to-cell variability seen in a larger population. We define cell-to-cell variationbased on the slopes of a linear regression model applied to capacity fade curves. Our methodology determinesa sample size which estimates this variability within user specified requirements on precision and *** sample size is found using the distributional properties of the slopes under a normality assumption, andan implementation of the approach is available on *** the five datasets in the study, we find that a sample size of 8–10 cells (at a prespecified precision andconfidence) captures the cell-to-cell variability of the larger datasets. We show that prior testing knowledge canbe leveraged with machine learning models to operationally optimise the design of new cell-testing, leadingup to a 75% reduction in experimental costs.

关键词： Battery Testing Lithium-ion Degradation Statistics Manufacturing Machine learning

Archean-Ediacaran evolution of the campos Gerais Domain-A reworked margin of the São Francisco paleocontinent(SE Brazil):constraints from metamafic-ultramafic rocks

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Geoscience Frontiers 2022年第5期13卷 286-308页

作者： Marco Aurélio Piacentini Pinheiro George L.Guice Joana reis Magalhães Geological Survey of Brazil–cPRM Av Brazil 1731Belo Horizonte 30140-002Brazil Department of Mineral Sciences National Museum of Natural HistorySmithsonian Institution10th Street and Constitution Avenue NWWashington D.C.20560USA

The campos Gerais Domain(cGD)in southeastern Brazil is an approximately 180 km×35 km area of Archean–Proterozoic rocks located southwest of the São Francisco craton(SFc).The Archean–Paleopro terozoic evolution of the cGD—alongside its potential correlation with the SFc or other cratonic blocks in the region-is currently *** present the results of systematic petrography,bulkrock geochemistry,mineral chemistry and geochronology for a suite of scarcely studied mafic–ultramafic rocks from the *** also provide a compilation of previously reported bulk-rock geochemical and spinel group mineral chemical data for mafic–ultramafic rocks throughout the cGD,and geochronological information for various lithotypes in the *** cGD records a protracted Mesoarchean to Statherian(3.1–1.7 Ga)crustal evolution,which we interpret to share a common history with the southern SFc and their related reworked segments,suggesting that it is a westward extension of this cratonic *** metavolcano-sedimentary rocks of the Fortaleza de Minas and Alpinópolis segments represent a Mesoarchean greenstone belt that is stratigraphically and chemically comparable to Archean greenstone belts worldwide,and that is broadly coeval with a local suite of tonalite-trondhjemite-grano diorite(TTG)gneisses and migmatites.U-Pb SHRIMP zircon data from a subalkaline metagabbro yielded a concordia age of ca.2.96 Ga,revealing a previously unrecognized phase of Archean magmatism in the cGD that can be chrono-correlated with metakomatiite and TTG generation elsewhere in the São Francisco *** data contradict a hypothesis whereby the metavolcano-sedimentary rocks of the Jacuí-Bom Jesus da Penha and Petúnia segments represent an ophiolite,as previously suggested,instead presenting features that point to formation in association with a continental *** with a U-Pb(SHRIMP)crystallization age of ca.2.13 Ga recorded by zircon grains from a metaultramafic rock,these data h

关键词： Komatiites São Francisco craton Southern Brasília Orogen Archean geodynamics Rhyacian orogeny

Shielding AZ91D-1%ca from corrosion through ultrasound melt treatment:A study for stent design

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Journal of Magnesium and Alloys 2023年第8期11卷 2901-2915页

作者： I.V.Gomes M.Pacheco M.Nienaber S.c.Neves D.Mei A.Barros R.L.reis J.L.Alves H.Puga cMEMS–UMinho University of MinhoGuimarãesPortugal LABBELS–Associate Laboratory BragaGuimarãesPortugal B's Research Group-Research Institute on Biomaterials Biodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineAvepark-Parque de Ciência e TecnologiaBarco GuimarãesPortugal IcVS/3B's-PT Government Associate Laboratory BragaGuimarãesPortugal Institute of Material and Process Design Helmholtz-Zentrum HereonGeesthacht 21502Germany School of Materials Science and Engineering&Henan Key Laboratory of Advanced Magnesium Alloy Zhengzhou UniversityZhengzhou 450001China

Magnesium-based materials show great potential for producing biodegradable stents,but their high corrosion rates are a *** study investigates whether ultrasound melt treatment can change the corrosion response of an extruded AZ91D-1.0%ca(wt.%)in Earle's Balanced Salt Solution by tailoring the intermetallics'morphology in the as-extruded *** results showed that the wires from ultrasound-treated ingots corroded faster than non-treated ones in immersion for up to 6 *** trend shifted for longer periods,and ultrasound-treated material showed lower corrosion rates and uniform corrosion,while the non-treated material displayed localized corrosion *** testing of the wires demonstrated that immersion in EBSS lowered the tensile strength and elongation at fracture due to material degradation,regardless of the processing ***,this decline was sharper in the non-treated *** findings suggest that ultrasound melt processing can be a promising method for improving the corrosion resistance of magnesium-based materials,paving the way for their use in manufacturing biodegradable stents.

关键词： Magnesium Ultrasound treatment Stent corrosion Mechanical properties

Adsorption of yttrium(Y^(3+)) and concentration of rare earth elements from phosphogypsum using chitin and chitin aerogel

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Journal of Rare Earths 2024年第4期42卷 775-782页

作者： Glaydson S.dos reis Diana Pinto Sabrina F.Lütke Eder c.Lima Luis F.O.Silva Irineu A.S.De Brum Guilherme L.Dotto Department of Forest Biomaterials and Technology Biomass Technology centre Swedish University of Agricultural SciencesUmeaE13Swedn Universidad De La costa Calle 58#55-66080002BarranquillaAtlanticoColombia Research Group on Adsorptive and catalytic Process Engineering(ENGEPAc) Federal University of Santa MariaAv.Roraima100079705900Santa MariaRSBrazil Institute of chemistry Federal University of Rio Grande do Sul90040060Porto AlegreBrazil

chitin powder and chitin-aerogel were prepared from shrimp wastes and used to uptake Y^(3+)from aqueous solutions and concentrate this rare earth element from phosphogypsum(PG).chitin aerogel displays a specific surface area of 945 m^(2)/g,while chitin powder is 3.6 m^(2)/g,which largely influences its adsorption *** the adsorption in synthetic solutions,the effect of pH on Y^(3+)removal is strong for chitin powder *** contrast,no big pH influence was detected for chitin *** interactions and chelation can highlight the proposed mechanism of Y^(3+)on chitin adsorbents for the powder and ***,in addition to these interactions,pore filling/pore diffusion is the main mechanism of Y^(3+)removal in the chitin *** aerogel is efficient in concentrating 8 times the Y^(3+)from PG,a very complex *** complex chitin aerogel-Y^(3+)can be a secondary source of rare earth elements for other applications.

关键词： Rare earths Sustainable material chitin powder chitin porous aerogel Yttrium adsorption and recovery

Significance of endothelial dysfunction in the pathogenesis of early and delayed radiation enteropathy

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World Journal of Gastroenterology 2007年第22期13卷 3047-3055页

作者： Junru Wang Marian Boerma Qiang Fu Martin hauer-Jensen Department of Surgery University of Arkansas for Medical Sciences United States Department of Pharmaceutical Sciences University of Arkansas for Medical Sciences United States Department of Microbiology and Immunology University of Arkansas for Medical Sciences United States Department of Surgery University of Arkansas for Medical Sciences Surgical Service Central Arkansas Veterans Healthcare System4 Little Rock AR United States

This review summarizes the current state of knowledge regarding the role of endothelial dysfunction in the pathogenesis of early and delayed intestinal radiation toxicity and discusses various endothelial-oriented interventions aimed at reducing the risk of radiation enteropathy. Studies published in the biomedical literature during the past four decades and cited in PubMed, as well as clinical and laboratory data from our own research program are reviewed. The risk of injury to normal tissues limits the cancer cure rates that can be achieved with radiation therapy. During treatment of abdominal and pelvic tumors, the intestine is frequently a major close-limiting factor. Microvascular injury is a prominent feature of both early （inflammatory）, as well as delayed （fibroproliferative） radiation injuries in the intestine and in many other normal tissues. Evidence from our and other laboratories suggests that endothelial dysfunction, notably a deficiency of endothelial thrombomodulin, plays a key role in the pathogenesis of these radiation responses. Deficient levels of thrombomodulin cause loss of vascular thromboresistance, excessive activation of cellular thrombin receptors by thrombin, and insufficient activation of protein c, a plasma protein with anticoagulant, anti-inflammatory, and cytoprotective properties. These changes are presumed to be critically involved in many aspects of early intestinal radiation toxicity and may sustain the fibroproliferative processes that lead to delayed intestinal dysfunction, fibrosis, and clinical complications. In conclusion, injury of vascular endothelium is important in the pathogenesis of the intestinal radiation response. Endothelial-oriented interventions are appealing strategies to prevent or treat normal tissue toxicity associated with radiation treatment of cancer.

关键词： Endothelial cells Thrombomodulin Proteinase-activated receptors Radiation injuries Radiation enteropathy