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Post cryopreservation Growth Kinetic and Photosynthetic Assessment of an Acid Tolerant Strain of Stichococcus bacillaris

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Natural Resources 2024年第5期15卷 130-148页

作者： Marzia licata Luigi Marra Nunzia Nappi Elena Aurino Feliciana Oliva Antonino De Natale Donato Giovannelli Antonino Pollio Department of Biology University of Naples &#8220Federico II&#8221 Naples Italy AcUF Algal Collection of University of Naples &#8220Federico II&#8221 Naples Italy Department of chemical Materials and Industrial Production Engineering University of Naples &#8220Federico II&#8221 P.le V. Tecchio Naples Italy Institute for Marine Biological Resources and Biotechnologies Italian National Research Council CNR-IRBIM Ancona Italy Earth-Life Science Institute ELSI Tokyo Institute of Technology Tokyo Japan Marine chemistry and Geochemistry Department Woods Hole Oceanographic Institution Woods Hole MA USA Department of Marine and coastal Science Rutgers University New Brunswick NJ USA

Preserving microbial diversity has become a strategic undertaking. Thus, ex situ microalgal culture conservation results in strategic and functional resource in both biodiversity protection and application domains. cryopreservation of microalgae has been practiced since the 1960s and is now considered the optimal preservation strategy. Furthermore, the overall monitoring during growth of cultures after freezing/thawing protocols was hardly investigated and there is poor evaluation related to preserve especially the photosystem apparatus. The present study focuses on Stichococcus bacillaris as case study for short-term cryopreservation at −80 °c storage. Various freezing pretreatments using cryoprotective agents, and two thawing methods were compared introducing a novel variable to evaluate viability recovery and assessing growth kinetics of cultures immediately after thawing and after a series batch cultivation. Photosynthetic rate and pigments assessment were proposed to evaluate hidden metabolic cell damage. Results underline cryoprotective agents can increase the kinetic recovery of preserved cells in terms of reduction of lag phase during batch cultivation tests: the use of dimethyl sulfoxide and glycerol granted a growth comparable to unpreserved cells when sudden thawing occurs after 24 hours of storage, but recovery after preservation is less sensitive to cryoprotective agents when gradual thawing and 1 month of storage is considered. However, cells are always able to restore their physiological pathways even without agents, so their kinetic effect has been proved and quantified. Interestingly, both the photosynthetic efficiency and the ratio between total chlorophyll and carotenoids are comparable (0.75 Fv/Fm, 2.2 ± 0.25 g/g) to unpreserved cells and they are unsensitive to chosen agents, but the ratio between chlorophyll a and chlorophyll b was clearly altered (up to 10 times), suggesting that photoactive pigments relative proportions

关键词： Microalgae Stichococcus bacillaris cryopreservation Growth Kinetic Photosynthetic Rate

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Measurement of the integrated luminosity of the Phase 2 data of the Belle Ⅱ experiment

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chinese Physics c 2020年第2期44卷 1-12页

作者： F.Abudinén I.Adachi P.Ahlburg H.Aihara N.Akopov A.Aloisio F.Ameli L.Andricek N.Anh Ky D.M.Asner H.Atmacan T.Aushev V.Aushev T.Aziz K.Azmi V.Babu S.Baehr S.Bahinipati A.M.Bakich P.Bambade Sw.Banerjee S.Bansal V.Bansal M.Barrett J.Baudot A.Beaulieu J.Becker P.K.Behera J.V.Bennett E.Bernieri F.U.Bernlochner M.Bertemes M.Bessner S.Bettarini V.Bhardwaj F.Bianchi T.Bilka S.Bilokin D.Biswas G.Bonvicini A.Bozek M.Bračko P.Branchini N.Braun T.E.Browder A.Budano S.Bussino M.campajola L.cao G.casarosa c.cecchi D.Červenkov M.-c.chang P.chang R.cheaib V.chekelian Y.Q.chen Y.-T.chen B.G.cheon K.chilikin H.-E.cho K.cho S.cho S.-K.choi S.choudhury D.cinabro L.corona L.M.cremaldi S.cunliffe T.czank F.Dattola E.De la cruz-Burelo G.De Nardo M.De Nuccio G.De Pietro R.de Sangro M.Destefanis S.Dey A.De Yta-Hernandez F.Di capua S.Di carlo J.Dingfelder Z.Doležal I.Domínguez Jiménez T.V.Dong K.Dort S.Dubey S.Duell S.Eidelman M.Eliachevitch T.Ferber D.Ferlewicz G.Finocchiaro S.Fiore A.Fodor F.Forti A.Frey B.G.Fulsom M.Gabriel E.Ganiev M.Garcia-Hernandez R.Garg A.Garmash V.Gaur A.Gaz U.Gebauer A.Gellrich J.Gemmler T.Geßler R.Giordano A.Giri B.Gobbo R.Godang P.Goldenzweig B.Golob P.Gomis P.Grace W.Gradl E.Graziani D.Greenwald c.Hadjivasiliou S.Halder K.Hara T.Hara O.Hartbrich K.Hayasaka H.Hayashii c.Hearty M.T.Hedges I.Heredia de la cruz M.Hernández Villanueva A.Hershenhorn T.Higuchi E.c.Hill H.Hirata M.Hoek S.Hollitt T.Hotta c.-L.Hsu Y.Hu K.Huang T.Iijima K.Inami G.Inguglia J.Irakkathil Jabbar A.Ishikawa R.Itoh M.Iwasaki Y.Iwasaki S.Iwata P.Jackson W.W.Jacobs D.E.Jaffe E.-J.Jang H.B.Jeon S.Jia Y.Jin c.Joo J.Kahn H.Kakuno A.B.Kaliyar G.Karyan Y.Kato T.Kawasaki H.Kichimi c.Kiesling B.H.Kim c.-H.Kim D.Y.Kim S.-H.Kim Y.K.Kim Y.Kim T.D.Kimmel K.Kinoshita c.Kleinwort B.Knysh P.Kodyš T.Koga I.Komarov T.Konno S.Korpar D.Kotchetkov N.Kovalchuk T.M.G.Kraetzschmar P.Križan R.Kroeger J.F.Krohn P.Krokovny W.Kuehn T.Kuhr M.Kumar R.Kumar K.Kumara S.Kurz A.Kuzmin Y.-J.Kwon S.lacaprara Y.-T.lai c.la licata K.lalwani L.lanceri J.S.lange K.lautenbach I.-S.Le Beihang University Beijing 100191 Brookhaven National laboratory UptonNew York 11973 Budker Institute of Nuclear Physics SB RAS Novosibirsk 630090 centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional Mexico City 07360 Faculty of Mathematics and Physics Charles University12116 Prague chiba University Chiba 263-8522 consejo Nacional de ciencia y Tecnología Mexico City 03940 Deutsches Elektronen–Synchrotron 22607 Hamburg Duke University DurhamNorth Carolina 27708 Earthquake Research Institute University of TokyoTokyo 113-0032 Forschungszentrum Jülich 52425 Jülich Department of Physics Fu Jen Catholic UniversityTaipei 24205 Key laboratory of Nuclear Physics and Ion-beam Application(MOE)and Institute of Modern Physics Fudan UniversityShanghai 200443 II.Physikalisches Institut Georg-August-Universität Göttingen37073 Göttingen Gifu University Gifu 501-1193 The Graduate University for Advanced Studies(SOKENDAI) Hayama 240-0193 Gyeongsang National University Jinju 52828 Department of Physics and Institute of Natural Sciences Hanyang UniversitySeoul 04763 High Energy Accelerator Research Organization(KEK) Tsukuba 305-0801 J-PARc Branch KEK Theory CenterHigh Energy Accelerator Research Organization(KEK)Tsukuba 305-0801 Indian Institute of Science Education and Research Mohali SAS Nagar140306 Indian Institute of Technology Bhubaneswar Satya Nagar 751007 Indian Institute of Technology Hyderabad Telangana 502285 Indian Institute of Technology Madras Chennai 600036 Indiana University BloomingtonIndiana 47408 Institute for High Energy Physics Protvino 142281 Institute of High Energy Physics Vienna 1050 Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049 Institute of Particle Physics(canada) VictoriaBritish Columbia V8W 2Y2 Institute of Physics Hanoi Instituto de Fisica corpusc.lar Paterna 46980 INFN laboratori Nazionali di Frascati I-00044 Frascati INFN Sezione di Napoli I-80126 Napoli INFN Sezione di Padova I-35131 Padova INFN Sezione di Perugia I-06123 Perugia

From April to July 2018,a data sample at the peak energy of the T(4 S) resonance was collected with the Belle Ⅱ detector at the SuperKEKB electron-positron *** is the first data sample of the Belle Ⅱ *** Bhabha and digamma events,we measure the integrated luminosity of the data sample to be(496.3±0.3±3.0) pb-1,where the first uncertainty is statistical and the second is *** work provides a basis for future luminosity measurements at Belle Ⅱ.

关键词： luminosity Bhabha digamma Belle II

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Genetic association of interleukin-6 polymorphism (-174 G/c) with chronic liver diseases and hepatocellular carcinoma

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World Journal of Gastroenterology 2013年第16期19卷 2449-2455页

作者： Lydia Giannitrapani Maurizio Soresi Daniele Balasus Anna licata Giuseppe Montalto Unit of Internal Medicine Biomedical Department of Internal Medicine and Specialties DiBiMIS University of Palermo

Interleukin-6 (IL-6) is a pleiotropic cytokine which is expressed in many inflammatory cells in response to different types of stimuli, regulating a number of biological processes. The IL-6 gene is polymorphic in both the 5' and 3' flanking regions and more than 150 single nucleotide polymorphisms have been identified so far. Genetic polymorphisms of IL-6 may affect the outcomes of several diseases, where the presence of high levels of circ.lating IL-6 have been correlated to the stage and/or the progression of the disease itself. The -174 G/c polymorphism is a frequent polymorphism, that is located in the upstream regulatory region of the IL-6 gene and affects IL-6 production. However, the data in the literature on the genetic association between the -174 G/c polymorphism and some specific liver diseases characterized by different etiologies are still controversial. In partic.lar, most of the studies are quite unanimous in describing a correlation between the presence of the high-producer genotype and a worse evolution of the chronic liver disease. This is valid for patients with hepatitis c virus (HcV)-related chronic hepatitis and liver cirrhosis and hepatocellu-lar carcinoma (Hcc) whatever the etiology. Studies in hepatitis B virus-related chronic liver diseases are not conclusive, while specific populations like non alcoholic fatty liver disease/non-alcoholic steatohepatitis, autoimmune and human immunodeficiency virus/HcV coinfected patients show a higher prevalence of the lowproducer genotype, probably due to the complexity of these clinical pictures. In this direction, a systematic revision of these data should shed more light on the role of this polymorphism in chronic liver diseases and Hcc.

关键词： Single nucleotide polymorphisms Interleukin-6 chronic hepatitis Liver cirrhosis Hepatocellular carcinoma

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clinical course and prognostic factors of hepatorenal syndrome:a retrospective single-center cohort study

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World Journal of Hepatology 2013年第12期5卷 685-691页

作者： Anna licata Marcello Maida Ambra Bonaccorso Fabio Salvatore Macaluso Maria cappello Antonio craxì Piero Luigi Almasio Section of Gastroenterology Dipartimento Biomedicodi Medicina Interna e SpecialisticaUniversity of Palermo

AIM: To investigate clinical and biochemical features of hepatorenal syndrome(HRS), to assess short and long- term survival evaluating potential predictors of early mortality. METHODS: Sixty-two patients with liver cirrhosis and renal failure, defined as a serum creatinine value > 1.5 mg/dL on at least two measurements within 48 h, admitted to our tertiary referral Unit from 2001 to 201, were retrospectively reviewed. Among them, 33 patients(53.2%) fulfilled the revised criteria of the International Ascites club for the diagnosis of HRS. Twenty-eight patients were treated with combinations of terlipressin and albumin, two with dopamine and al- bumin, and three with albumin alone. No patients were suitable for liver transplantation. complete response was defined as normalization of creatinine levels to less than 1.5 mg/dL, partial response as a decrease of at least 50% but not to less than 1.5 mg/dL, no response as no reduction in creatinine or a decrease of less 50% compared to pre-treatment values. All of the patients were followed up for at least 1 year until January 2013. RESULTS: HRS type 1 was diagnosed in 15 patients(45.5%). Hepatitis c virus infection was the primary etiology(69.6%), followed by alcohol(15.2%), and cryptogenesis(15.2%). complete response to therapy was obtained in only 3 cases(9.1%) and partial re- sponse in 7 patients(21.2%). Median survival was 30 d(range: 10-274) without significant differences be- tween type 1 and type 2 HRS. By univariate analysis, child-Pugh class c(P = 0.009), presence of hepatocel- lular carcinoma(P = 0.04), low serum sodium(P = 0.02), high bilirubin values(P = 0.009) and high Model for End-stage Liver Disease(MELD) score(P = 0.03) were predictive factors of 30-d mortality. By multivari- ate analysis, only serum sodium core > 27(OR = 18.72; P = 0.01) were independently associated with a survival of less than one month. cONcLUSION: HRS still has a poor prognosis, even when va

关键词： Hepatorenal syndrome Liver cirrhosis Hepatitis c virus vasoactive drugs Mortality

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比索洛尔与氯沙坦服药一年后对新诊断高血压患者心肾功能的影响

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中华高血压杂志 2011年第12期19卷 1126-1128页

作者： Parrinello G Paterna S Torres D Di Pasquale P Mezzero M la Rocca G cardillo M Trapanese c caradonna M licata G One-year renal and cardiac effects of bisoprolol versus losartan in recently diagnosed hypertensive patients~ a randomized double-blind study.

高血压是慢性肾损伤的重要原因,抗高血压治疗能够降低肾功能衰竭的发生率。曾有报道认为β受体阻断剂可能诱发肾功能恶化,同时也有数据表明血管紧张素受体拮抗剂氯沙坦有肾脏保护作用。此前在比索洛尔与肾功能之间相互关系的研究只进行... 详细信息

高血压是慢性肾损伤的重要原因,抗高血压治疗能够降低肾功能衰竭的发生率。曾有报道认为β受体阻断剂可能诱发肾功能恶化,同时也有数据表明血管紧张素受体拮抗剂氯沙坦有肾脏保护作用。此前在比索洛尔与肾功能之间相互关系的研究只进行了短期或中期给药。这项研究的目的是比较比索洛尔与氯沙坦在高血压患者中用药一年后抗高血压的疗效及对肾脏与心脏血流动力学的影响。

关键词：高血压患者比索洛尔心肾功能氯沙坦血管紧张素受体拮抗剂抗高血压治疗肾脏保护作用心脏血流动力学

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current Status of Biogas Production, Upgrading Potential and Impact of Biomethane Use for Transport in Italy

Current Status of Biogas Production, Upgrading Potential and...

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The 18th European Biomass conference and Exhibition

作者： cocchi M. capaccioli S. Bazzoffi F. Grassi A. Sagnelli F. la licata B. laitinen T.

This report is set in the framework of the Intelligent Energy for Europe program project called "Gas Highway", launched in 2009 with the purpose of the promotion of the "Uptake of Gaseous Vehicle Fuels, Biogas and Natural Gas, in Europe". The project has started in May 2009 and will run for 3 years under the coordination of Jyv?skyl? Innovation,（Finland） and the participation of 14 other partners from Italy, Sweden, Estonia, Poland, czech Republic, Germany, Austria, Spain, latvia, and Lithuania. Biogas production has been found to be among the most environmentally benign and energy efficient means of producing vehicle biofuel from waste resources, whereas the use of natural gas as vehicle fuel also decreases the carbon dioxide emissions of vehicles by about one fourth compared to those of traditional petrol fuelled cars. The production of biogas as a sustainable alternative for methane fuel is getting large consideration in Italy. One of the possibilities, in addition to the power generation sector, is the upgrading of biogas to the quality of natural gas for a subsequent injection into the grid.

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Hyperferritinemia is a risk factor for steatosis in chronic liver disease

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World Journal of Gastroenterology 2009年第17期15卷 2132-2138页

作者： Anna licata Maria Elena Nebbia Giuseppe cabibbo Giovanna Lo Iacono Francesco Barbaria Virna Brucato Nic.la Alessi Salvatore Porrovecchio Vito Di Marco Antonio craxì calogero cammà Gastroenterology and Hepatology Unit Department of Internal Medicine University of Palermo 90127 Palermo Italy

AIM: To investigate the relationship between ferritin and steatosis in patients with chronically abnormal liver function tests (LFTs) and high ferritin level. METHODS: One hundred and twenty-four consecutive patients with hyperferritinemia (male > 300 ng/mL, female > 200 ng/mL) were evaluated; clinical, biochemical and serological data, iron status parameters, HFE gene mutations and homeostasis model assessment score were obtained. Steatosis was graded by ultrasound as absent or present. Histology was available in 53 patients only. RESULTS: Mean level of ferritin was 881 ± 77 ng/mL in men and 549 ± 82 ng/mL in women. The diagnosis was chronic hepatitis c in 53 (42.7%), non-alcoholic fatty liver disease/non-alcoholic steatohepatitis in 57 (45.9%), and cryptogenic liver damage in 14 (11.3%). None was diagnosed as hereditary hemochromatosis (HH). Hepatic siderosis on liver biopsy was present in 17 of 54 (32%) patients; grade 1 in eight and grade 2 in nine. Overall, 92 patients (74.2%) had steatosis. By logistic regression, ferritin and γ-glutamyltransferase were independent predictors of steatosis. Ferritin levels were signifi cantly related to low platelet count, steatosis and hepatitis c virus infection. cONcLUSION: In a non-obese cohort of non-alcoholic patients with chronically abnormal LFTs without HH, high serum ferritin level is a risk factor for steatosis.

关键词： Steatosis Serum ferritin chronic liverdisease Hepatitis c γ-glutamyltransferase

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