检索结果-南通市图书馆

Generation of Eco-Friendly and Disease-Resistant channel catfish(Ictalurus punctatus)Harboring the Alligator cathelicidin Gene via cRISPR/cas9 Engineering

在线全文

学校读者我要写书评

暂无评论

Engineering 2024年第8期39卷 273-286页

作者： jinhai Wang Baofeng Su De Xing Timothy j.Bruce Shangjia Li Logan Bern Mei Shang Andrew johnson Rhoda Mae c.Simora Michael coogan Darshika U.Hettiarachchi Wenwen Wang Tasnuba Hasin jacob Al-Armanazi cuiyu Lu Rex A.Dunham School of Fisheries Aquacultureand Aquatic SciencesAuburn UniversityAuburnAL 36849USA

As a precise and versatile tool for genome manipulation,the clustered regularly interspaced short palindromic repeats(cRISPR)/cRISPR-associated protein 9(cas9)platform holds promise for modifying fish traits of *** the aim of reducing transgene introgression and controlling reproduction,upscaled disease resistance and reproductive intervention in catfish species have been studied to lower the potential environmental risks of the introgression of escapees as transgenic *** advantage of the cRISPR/cas9-mediated system,we succeeded in integrating the cathelicidin gene(As-cath)from an alligator(Alligator sinensis)into the target luteinizing hormone(lh)locus of channel catfish(Ictalurus punctatus)using two delivery systems assisted by double-stranded DNA(dsDNA)and single-stranded oligodeoxynucleotides(ssODNs),*** this study,high knock in(KI)efficiency(22.38%,64/286)but low ontarget events was achieved using the ssODN strategy,whereas adopting a dsDNA as the donor template led to an efficient on-target KI(10.80%,23/213).The on-target KI of As-cath was instrumental in establishing the lh knockout(LH^(–)_As-cath^(+))catfish line,which displayed heightened disease resistance and reduced fecundity compared with the wild-type(WT)sibling ***,administration of human chorionic gonadotropin(HcG)and luteinizing hormone-releasing hormone analogue(LHRHa)can restore the reproduction of the transgenic fish ***,we replaced the lh gene with an alligator cathelicidin transgene and then administered hormone therapy to move towards complete reproductive control of diseaseresistant transgenic catfish in an environmentally responsible *** strategy not only effectively improves consumer-valued traits but also guards against unwanted introgression,providing a breakthrough in aquaculture genetics to confine fish reproduction and prevent the establishment of transgenic or domestic genotypes in the natural environment.

关键词： Genome editing ssODN dsDNA Antimicrobial peptide Reproductive confinement Aquaculture

Further investigation on the fusion of ^(6)Li with ^(209)Bi target at near-barrier energies

在线全文

学校读者我要写书评

暂无评论

chinese Physics c 2024年第7期48卷 106-117页

作者：张高龙焦振威张广鑫 M.R.cortes 胡世鹏钱建强 D.Mengoni 屈卫卫李聪博郑云张焕乔孙慧斌王楠张春雷 j.j.Valiente-Dobón D.Testov M.Mazzocco A.Gozzelino c.Parascandolo D.Pierroutsakou M.La commara A.Goasduff D.Bazzacco D.R.Napoli F.Galtarossa F.Recchia A.Illana S.Bakes I.Zanon S.Aydin G.de Angelis M.Siciliano R.Menegazzo S.M.Lenzi j.L.Ferreira j.Rangel Serkan Akkoyun L.F.canto j.Lubian School of Physics Beihang UniversityBeijing 100191China Sino-French Institute of Nuclear Engineering and Technology Sun Yat-Sen UniversityZhuhai 519082China Instituto de Física Universidade Federal Fluminense24210-340NiteróiRio de JaneiroBrazil Institute for Advanced Study in Nuclear Energy&Safety Shenzhen UniversityShenzhen 518060China Shenzhen Key Laboratory of Research and Manufacture of High Purity Germanium Materials and Fetectors Shenzhen UniversityShenzhen 518060China INFN Sezione di PadovaPadovaItaly Dipartimento di fisica Astronomia dell'Universita di padova PadovaItaly State Key Laboratory of Radiation Medicine and Protection School of Radiation Medicine and ProtectionSoochow UniversitySuzhou 215123China china Institute of Atomic Energy Beijing 102413China The Key Laboratory of Beam Technology and Material Modification of Ministry of Education College of Nuclear Science and TechnologyBeijing Normal UniversityBeijing 100875China INFN Laboratori Nazionali di LegnaroI-35020 LegnaroItaly INFN Sezione di NapoliI-80126 NapoliItaly Dipartimento di Farmacia Universitàdi Napoli"Federico II"I-80131 NapoliItaly Department of Natural and Mathematical Sciences Faculty of EngineeringTarsus University33480 MersinTurkiye Physics Division Argonne National LaboratoryLemont-ILUSA Departamento de Matemaética Física e Computaç˜ao Universidade do Estado do Rio de JaneiroFaculdade de Tecnologia27537-000ResendeRio de JaneiroBrazil Department of Physics Sivas Cumhuriyet UniversitySivasTurkey Instituto de Física Universidade Federal do Rio de JaneiroCP 6852821941-972Rio de JaneiroBrazil

The complete and incomplete fusion cross sections for ^(6)Li+^(209)Bi were measured using the in-beamγ-ray method around the coulomb *** cross sections of(deuteron captured)incomplete fusion(IcF)products were re-quantified experimentally for this reaction *** results reveal that the IcF cross section is equivalent to that of complete fusion(cF)above the coulomb barrier and dominant near or below the coulomb barrier.A theoretical calculation based on the continuum discretized coupled channel(cDcc)method was performed for the aforementioned cF and IcF cross sections;the result is consistent with the *** universal fusion function(UFF)was also compared with the measured cF cross section for different barrier parameters,demonstrating that the cF suppression factor is significantly influenced by the choice of potential,which can reflect both dynamic and static effects of breakup on the fusion process.

关键词： cross section incomplete fusion suppression factor weakly bound nuclei

Adaptive neural network control of a 2-DOF helicopter system considering input constraints and global prescribed performance

在线全文

学校读者我要写书评

暂无评论

Science china(Information Sciences) 2024年第7期67卷 224-239页

作者： Zhijia ZHAO jiale WU Zhijie LIU We HE c.L.Philip cHEN School of Mechanical and Electrical Engineering Guangzhou University School of Intelligence Science and Technology and Key Laboratory of Intelligent Bionic Unmanned Systems of Ministry of Education University of Science and Technology Beijing School of computer Science and Engineering South China University of Technology Pazhou Lab

In this study, an adaptive neural network(NN) control is proposed for nonlinear two-degree-offreedom(2-DOF) helicopter systems considering the input constraints and global prescribed ***, radial basis function NN(RBFNN) is employed to estimate the unknown dynamics of the helicopter system. Second, a smooth nonaffine function is exploited to approximate and address nonlinear constraint functions. Subsequently, a new prescribed function is proposed, and an original constrained error is transformed into an equivalent unconstrained error using the error transformation and barrier function transformation methods. The analysis of the established Lyapunov function proves that the controlled system is globally uniformly bounded. Finally, the simulation and experimental results on a constructed Quanser's test platform verify the rationality and feasibility of the proposed control.

关键词： adaptive NN control 2-DOF helicopter global prescribed performance input constraints

Malargüe Site Remediation:A Successful Solution for Uranium Mill Tailings

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

原子能科学技术 2024年第10期58卷 2088-2094页

作者： BARARI E DíAZ j G GIOMIA KEMPF R A National Atomic Energy commission Av.Del Libertador 8250Ciudad Autónoma de Buenos AiresC1429BNPArgentina

comisión Nacional de Energía Atómica (cNEA) has the responsibility for restoring uranium mining facilities once the operations have ***,within its Environmental Program and in compliance with its legal responsibilities,decides to implement a restoration project for all sites related to the mining and processing of uranium *** Malargüe Site is located within the Province of Mendoza in the city of Malargü*** is the first site to successfully complete its *** activities consist of relocation of tailings to an engineering *** tailings management(encapsulation) and rehabilitation of the area was finished in june *** remediation alternative for the ore tailings was selected after conducting comparative studies and submitted the project to the society for *** objective of the encapsulation of the mineral tails is to isolate them from the environment,also proceeding with the decontamination and rehabilitation of the area (landscaping,post-closure monitoring and 20 years monitoring period).Encapsulation consisted of the construction of a containment cell for the mine tailings,to isolate them and prevent pollutants from entering the environment through the transfer *** clean the impacted areas,the soil was removed,it was incorporated into the encapsulation,and the filling was carried out with natural soils from the *** prevents radon transfer to the environment,as ^(222)Ra is an alpha emitter with a half-life of four days,which produces its own radioactive *** progeny are solids,and when a ^(222)Ra nucleus emits an alpha particle into the air,the resulting ^(218)Po nucleus,momentarily electrically charged,adheres to any dust *** prevents the discharge into the air containing radon and also containing dust particles charged with intensely radioactive radon *** tasks mentioned make it possible to decrease radon emanation,reduce radiological risks to the public and pre

关键词： remediation radionuclide transfer tailings encapsulation solution sustainable

Developing a new agenda for increased food and climate security

在线全文

学校读者我要写书评

暂无评论

Frontiers of Agricultural Science and Engineering 2024年第1期11卷 35-54页

作者： William j.DAVIES Lancaster Environment centre Lancaster UniversityLancasterLA14YQUK

In many countries, political and environmental pressures are currently combining to generate a perfect storm of circumstances that is reducing food availability, increasing food costs and thereby reducing the availability of food to many. The UK is currently considering new national food and land management policies, and attention is also being given to legislation to address diet-related health issues. Many now argue for a revolution in UK farming practices to reduce their impact on the natural environment. The UK is not alone in facing these and other challenges. Both the contribution of agriculture to greenhouse gas(GHG) emissions and the effects of climate change on food production are issues receiving worldwide *** agricultural practices can result in greater c capture, reduced GHG emissions, enhanced soil quality and enhanced biodiversity. However, it is questioned if such farming systems will be productive enough to feed a growing population with the food required for social and health benefits. To fully exploit the impact of new plant science in farmer fields, it is imperative to effectively link science to farming practices and conduct a broader conversation around the food revolution with social scientists and with the general public.

关键词： climate resilience food security human health planetary health regenerative agriculture

complexed amino acid minerals vs. bis-glycinate chelated minerals:Impact on the performance of old laying hens

在线全文

学校读者我要写书评

暂无评论

Animal Nutrition 2024年第1期16卷 395-408页

作者： Marcos j.B.Santos Maria c.M.M.Ludke Leandro M.Silva carlos B.V.Rabello Mércia R.Barros Fabiano S.costa clariana S.Santos jamille S.S.Wanderley Department of Animal Science Rural Federal University of Pernambuco Department of Veterinary Science Rural Federal University of Pernambuco

The present study was to evaluate the effect of trace minerals(Zn, Mn, and cu) from complexed amino acid minerals(ZMcAA) and bis-glycinate chelated minerals(ZMcGly) in laying hen diets on performance,internal and external egg quality, yolk mineral deposition, intestinal morphometry, and bone characteristics. From 78 to 98 weeks of age, 400 White LSL-Lite strain laying hens were distributed in a randomized design with 4 treatments with 10 replicates per treatment. Treatments were distributed in a 2 ×2 factorial arrangement using either Zn, Mn, and cu of ZMcAA or ZMcGly source at 2 levels: low(20, 20,and 3.5 mg/kg of Zn, Mn, and cu, respectively) or high(40, 40, and 7 mg/kg of Zn, Mn, and cu,respectively). The analysis of variance was performed, and in cases where differences were observed, the means were compared using Tukey?s test(P ce and level of trace mineral supplementation had a significant impact on the performance of laying hens. Hens fed ZMcAA had higher egg production(P = 0.01), egg weight(P = 0.02), egg mass(P = 0.01), and lower feed conversion ratio(P =0.05) compared to those fed ZMcGly. The ZMcAA supplementation showed higher albumen height(P =0.01), albumen weight(P = 0.01), and eggshell thickness(P cu(P ceived ZMcAA. Tibia weight(P = 0.04)and bone densitometry(P cAA supplementation. In the small intestine, ZMcAA resulted in longer villi(P = 0.02) and shorter crypt depth(P = 0.01) in the *** and ileum measurements were influenced by the level and source of trace minerals(P cAA exhibited superior performance, egg quality, deposition of trace minerals in the egg yolk, and bone density compared to hens fed ZMcGly. In this study, older laying hens supplemented with ZMcAA at lower levels demonstrated adequate levels of supplementation.

关键词： Bis-glycinates Metal-amino acids Radiodensity Trace minerals

A Study of the TPS Based Beam-Matching concept for Medical Linear Accelerators at a Tertiary Hospital

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

International journal of Medical Physics, clinical Engineering and Radiation Oncology 2024年第1期13卷 16-25页

作者： Ntombela N. Lethukuthula Rovetto j. Nicolas Nethwadzi c. Lutendo Mpumelelo Nyathi Department of Radiation Oncology Steve Biko Academic Hospital Pretoria South Africa Department of Medical Physics University of Pretoria Pretoria South Africa Department of Medical Physics Sefako Makgatho Health Sciences University Ga-Rankuwa South Africa

The flexibility in radiotherapy can be improved if patients can be moved between any one of the department’s medical linear accelerators (LINAcs) without the need to change anything in the patient’s treatment plan. For this to be possible, the dosimetric characteristics of the various accelerators must be the same, or nearly the same. The purpose of this work is to describe further and compare measurements and parameters after the initial vendor-recommended beam matching of the five LINAcs. Deviations related to dose calculations and to beam matched accelerators may compromise treatment accuracy. The safest and most practical way to ensure that all accelerators are within clinical acceptable accuracy is to include TPS calculations in the LINAcs matching evaluation. Treatment planning system (TPS) was used to create three photons plans with different field sizes 3 × 3 cm, 10 × 10 cm and 25 × 25 cm at a depth of 4.5 cm in Perspex. calculated TPS plans were sent to Mosaiq to be delivered by five LINAcs. TPS plans were compared with five LINAcs measurements data using Gamma analyses of 2% and 2 mm. The results suggest that for four out of the five LINAcs, there was generally good agreement, less than a 2% deviation between the planned dose distribution and the measured dose distribution. However, one specific LINAc named “Asterix” exhibited a deviation of 2.121% from the planned dose. The results show that all of the LINAcs’ performance were within the acceptable deviation and delivering radiation dose consistently and accurately.

关键词： Radiotherapy Beam-Matching Linear Accelerator Dosimetry

Important step towards an understanding of the exotic Zc（3900）

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science china(Physics,Mechanics & Astronomy) 2024年第9期Mechanics & Astronomy) .卷 203-204页

作者： c.Hanhart Institute for Advanced Simulation Forschungszentrum Jülich

The Standard Model of particle physics, which contains the field theories for the electro-weak interaction(QED) and the strong interaction(QcD), is an extremely successful theory describing a huge variety of phenomena at the atomic and subatomic level with incredible accuracy. However, the inner workings of the strong interaction for systems characterised by small momentum transfers(of the order of ΛQcDor smaller) are still not fully understood.

关键词：

Plant triterpenoid saponins function as susceptibility factors to promote the pathogenicity of Botrytis cinerea

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2024年第7期17卷 1073-1089页

作者： Francisco j.Escaray Amelia Felipo-Benavent cristian j.Antonelli Begona Balaguer Maria Pilar Lopez-Gresa Pablo Vera Instituto de Biologia Molecular y celular de Plantas Universidad Politecnica de Valencia-C.S.l.CCiudad Politecnica de la InnovacionEdificio 8Eacceso GIngeniero Fausto Elios/n46022ValenciaSpain

The gray mold fungus Botrytis cinerea is a necrotrophic pathogen that causes diseases in hundreds of plant species,including high-value *** polyxenous nature and pathogenic success are due to its ability to perceive host signals in its *** this study,we found that laticifer cells of Euphorbia lathyris are a source of susceptibility factors required by *** to cause ***,poor-in-latex(pil)mutants,which lack laticifer cells,show full resistance to this pathogen,whereas lot-of-latex mutants,which produce more laticifer cells,are *** S factors are triterpenoid saponins,which are widely distributed natural products of vast structural *** downregulation of laticifer-specific oxydosqualene cyclase genes,which encode the first committed step enzymes for triterpene and,therefore,saponin biosynthesis,conferred disease resistance to ***,the Medicago truncatula Iha-1 mutant,compromised in triterpenoid saponin biosynthesis,showed enhanced ***,the application of different purified triterpenoid saponins pharmacologically complemented the diseaseresistant phenotype ofpil and hla-1 mutants and enhanced disease susceptibility in different plant *** found that triterpenoid saponins function as plant cues that signal transcriptional reprogramming in ***,leading to a change in its growth habit and infection strategy,culminating in the abundant formation of infection cushions,the multicellular appressoria apparatus dedicated to plant penetration and biomass destruction in *** together,these results provide an explanation for how plant triterpenoid saponins function as disease susceptibility factors to promote *** pathogenicity.

关键词： Euphorbia lathyris laticifer cell latex appressorium infection cushion necrotrophic fungi