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Effects of La^(3+) and Gd^(3+) on ca^(2+) influx in rat hepatoma H-35 cells

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chinese Science Bulletin 1999年第4期44卷 331-335页

作者： WONG Patrick c. L. department of Biochemistry The University of Hong Kong

Effects of La93+) and Gd3+ on ca2+ influx were investigated in rat hepatoma H-35 cells by measuring the initial rate of45ca2+ uptake. It was found that the maximum initial rate of ca2+ uptake was increased six- to ten-fold at low concentrations of La3+ and Gd3+. Kinetic analyses by measuring the initial rate of ca2+ influx at different external ca2+ concentrations indicated the existence of two intracellular exchangeable components in the basal ca2+ system, with low and high affinities for ca2+,and only one class of ca2+ binding sites was observed in the La3+- or Gd3+-treated cells. For high affinity, La3+ and Gd3+ increased both kinetic parameters Km and V(max of basal ca2+ influx. La3+ and Gd3+ compete directly with ca2+ for ca2+ binding site for low affinity. The kinetics is competitive.

关键词： La3+ Gd3+ rat hepatoma H-35 cells ca2+ influx.

current protective strategies in liver surgery

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World Journal of Gastroenterology 2010年第48期16卷 6098-6103页

作者： Kurinchi S Gurusamy Hector d Gonzalez Brian R davidson HPB and Liver Transplantation Royal Free CampusUniversity College London Medical SchoolRoyal Free Hospital

during liver resection surgery for cancer or liver transplantation,the liver is subject to ischaemia (reduction in blood flow) followed by reperfusion (restoration of blood flow),which results in liver injury [ischemiareperfusion (IR) or IR injury]. Modulation of IR injury can be achieved in various ways. These include hypothermia,ischaemic preconditioning (IPc) (brief cycles of ischaemia followed by reperfusion of the organ before the prolonged period of ischaemia i.e. a conditioning response),ischaemic postconditioning (conditioning after the prolonged period of ischaemia but before the reperfusion),pharmacological agents to decrease IR injury,genetic modulation of IR injury,and machine perfusion (pulsatile perfusion). Hypothermia decreases the metabolic functions and the oxygen consumption of organs. Static cold storage in University of Wisconsin solution reduces IR injury and has prolonged organ storage and improved the function of transplanted grafts. There is currently no evidence for any clinical advantage in the use of alternate solutions for static cold storage. Although experimental data from animal models suggest that IPc,ischaemic postconditioning,various pharma-cological agents,gene therapy,and machine perfusion decrease IR injury,none of these interventions can be recommended in clinical practice. This is because of the lack of randomized controlled trials assessing the safety and efficacy of ischaemic postconditioning,gene therapy,and machine perfusion. Randomized controlled trials and systematic reviews of randomized controlled trials assessing the safety and efficacy of IPc and various pharmacological agents have demonstrated biochemical or histological improvements but this has not translated to clinical benefit. Further well designed randomized controlled trials are necessary to assess the various new protective strategies in liver resection.

关键词： Liver Hepatectomy Liver transplantation Ischemia-reperfusion injury Hypothermia Ischaemic preconditioning

The Origin and Tectonic Setting of Precambrian Greywacke of Ribandar-chimbel, Goa, India： Petrological and Geochemical Evidence

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Acta Geologica Sinica(English Edition) 2016年第6期90卷 2036-2048页

作者： Glancia Q FERNANdES Sridhar d. IYER Mahender KOTHA department of Earth Science Goa University Taleigao Plateau Goa 403206 India department of civil Engineering Don Bosco College of Engineering Fatorda Margao Goa 403602 India cSIR-National Institute of Oceanography Dona Paula Goa 403004 India

The Precambrian greywacke of Ribandar-chimbel belonging to the Sanvordem Formation of the Goa Group, India, has been studied for petrography and analyzed for major trace elements. The greywacke is characterized by angular to sub-round grains of quartz, feldspar, biotite, chlorite and clay minerals. The abundance of clay in the matrix seems to have influenced the Al2O3 content and the K20/Al2O3 ratio. The variation diagrams indicate a decreasing trend of TiO2, Al2O3, Fe2O3 and MgO; whereas Na2O and caO exhibit a scatter which could be a result of the variable presence of feldspar within the sediments. The immobile elements, vanadium （25 to 144 ppm）, nickel （up to 107 ppm） and chromium （up to 184 ppm）, reflect abundance of clay minerals. The greywacke shows strongly fractionated REE patterns with LaN/YbN = 8 to 26 and with higher total REE abundances （up to 245 ppm）. The low REE enrichment and depletion in heavier REE with prominent negative Eu anomaly （Eu/Eu^＊= 0.54 to 0.79） suggest a derivation of the greywacke from an old upper continental crust composed chiefly of felsic components. Petrological evidence and geochemical data suggest that the deposition of the greywacke largely took place in a deep to shallow basin that progressively chang- ed from that of a continental island arc to an active continental setting.

关键词： greywacke petrochemistry origin diagenesis active continental setting Goa dharwar craton

QFT preflter design for multivariable systems using interval constraint satisfaction technique

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控制理论与应用（英文版） 2013年第4期11卷 529-537页

作者： Mukesh d.PATIL P.S.V.NATARAJ department of Electronics Engineering Ramrao Adik Institute of Technology Systems and control Engineering Indian Institute of Technology Bombay

In this paper, a computationally efficient method is proposed for automated design of the prefilters for multivariable systems. In quantitative feedback theory （QFT） method, proposed by Horowitz, the prefilter is designed to achieve the desired tracking specifications. In the proposed approach, we pose the prefilter design problem as an interval constraint satisfaction problem and solve it using the well-established interval constraint satisfaction techniques. The proposed method finds optimal values of the parameters of fixed structure prefilter within the initial search domain. An approach based on prefilter synthesis for single-input single-output is already developed. The purpose of this paper is to extend this approach to QFT prefilter design for general multivariable systems. To validate the above design approach, we applied the method to a laboratory setup of magnetic levitation system.

关键词： Interval constraint satisfaction technique Prefilter design Quantitative feedback theory

Keratoconus in a patient with Alport syndrome: A case report

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World Journal of clinical cases 2019年第19期7卷 3012-3017页

作者： Majid Moshirfar david F Skanchy Aaron T Gomez Yasmyne c Ronquillo Benjamin Buckner Phillip c Hoopes John A.Moran Eye center Department of Ophthalmology and Visual SciencesUniversity of Utah School of MedicineSalt Lake CityUT 84101United States Utah Lions Eye Bank Salt Lake CityUT 84101United States Hoopes durrie Rivera Research center Hoopes VisionDraperUT 84020United States Kellogg Eye center Department of OphthalmologyUniversity of Michigan Medical SchoolAnn ArborMI 48103United States The University of Texas Rio Grande Valley School of Medicine EdinburgTX 78542United States

BAcKGROUNd Known ocular manifestations of Alport syndrome include features such as anterior lenticonus and fleck retinopathy. Reports of keratoconus in such patients are limited. We report tomographic findings consistent with keratoconus in a patient with Alport *** SUMMARY A 52-year-old female was referred to our ophthalmology clinic with decreased vision and increased tearing. She was diagnosed with stage Ⅲ Alport syndrome two years prior. Upon examination she was found to have average keratometries of 48d bilaterally with tomographic evidence of *** Although a rare presentation, concurrent Alport syndrome and keratoconus should be considered when reviewing the ocular health of Alport syndrome patients and appropriate management steps should be taken upon the diagnosis.

关键词： Alport syndrome,Keratoconus Type Ⅳ collagen cOL4A genes corneal ectasia case report

characterization of the Tomato Prosystemin Promoter:Organ-specific Expression,Hormone Specificity and Methyl Jasmonate Responsiveness by deletion Analysis in Transgenic Tobacco Plants

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Journal of Integrative Plant Biology 2012年第1期54卷 15-32页

作者： Hamlet Avilés-Arnaut John Paul délano-Frier center of Research and Advanced Studies (cinvestav) at Irapuato:Unit for Plant Biotechnology and Genetic Engineering

Tomato systemin is a bioactive peptide that regulates the systemic activation of wound-responsive genes. It is released from its 200 amino acid precursor called prosystemin. Initial tissue-localization and hormone-induced expression assays indicated that the tomato prosystemin gene （SIPS） accumulates mainly in floral tissues and in response to exogenous abscisic acid and methyl jasmonate （MeJA） treatments, respectively. Later, the promoter regions of the PS gene in tomato （Solanum lycopersicum L. cv. castlemart）, pepper （capsicum annuum） and potato （Solanum tuberosum） were isolated and an in silico analysis of the SIPS promoter revealed an over-representation of stress- and MeJA-responsive motifs. A subsequent 5＇ deletion analysis of the SIPS promoter fused to the/^-glucuronidase reporter （GUS） gene showed that the -221 to ＋40 bp proximal SIPS promoter region was sufficient to direct the stigma, vascular bundle-specific and MeJA-responsive expression of GUS in transgenic tobacco plants. Important ***-specific, light- and MeJA-responsive cis-elements were also present in this region. These findings provide relevant information regarding the transcriptional regulation mechanisms of the SIPS promoter operating in transgenic tobacco plants. They also suggest that its Ussue-specificity and inducible nature could have wide applicability in plant biotechnology.

关键词： cis-regulatory elements flower specific jasmonic acid methyl jasmonate promoter analysis prosystemin wound-responsive genes.

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染料敏化太阳电池光电极厚度的研究

电源技术 2006年第11期30卷 917-920,924页

作者：倪萌 Leung M.K.H. Leung d.Y.c. 香港大学机械工程系香港

根据电子在半导体中的扩散理论，建立了模拟染料敏化太阳电池（dSSc）J-V输出特性的数学模型。研究表明，随着光电极厚度（d）的增大，开路电压（VOc）下降：短路电流密度（JSc）先迅速增大。后缓慢减小：最大功率输出值MPP则先迅速增... 详细信息

根据电子在半导体中的扩散理论，建立了模拟染料敏化太阳电池（dSSc）J-V输出特性的数学模型。研究表明，随着光电极厚度（d）的增大，开路电压（VOc）下降：短路电流密度（JSc）先迅速增大。后缓慢减小：最大功率输出值MPP则先迅速增大，再迅速减小，表明d对dSSc光电转化效率影响显著。对dSSc的各参数进行了分析，发现操作参数，如光强、温度，对最佳电极厚度的确定没有影响，而物性参数，如光吸收系数、电子扩散系数及电子生命则决定了dSSc光电极厚度的大小。将模型结果与文献实验结果进行了对比，两者符合良好。说明模型结果可靠，可以为dSSc的优化设计提供理论依据。

关键词：染料敏化太阳电池(dSSc) 最大功率输出值MPP 光电极厚度

Nonintubated thoracic surgery: a lead role or just a walk on part?

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chinese Journal of cancer Research 2014年第5期26卷 507-510页

作者： Tommaso c.Mineo Federico Tacconi Thoracic Surgery division Research Unit on Non-General Anesthesia in Thoracic Surgery00133Tor Vergata University

With the advent of the patient-centered health care concept, thoracic surgeons of the modern era are called to minimize at most the overall invasiveness of their performances, while increasing safety and effectiveness as much as possible. This need has driven an outstanding development of minimallyinvasive surgical approaches, which are now the standard to perform a broad spectrum of thoracic operations. A recent and fascinating evolution in this field is represented by the increasing interest toward the use of so-called ＂nonintubated＂ anesthesia protocol, which are expected to provide a further improvement in postsurgical outcomes （1-8）.

关键词： a lead role or just a walk on part Nonintubated thoracic surgery

Base editing:a brief review and a practical example

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The Journal of Biomedical Research 2021年第2期35卷 107-114页

作者： dongwook c.choe Kiran Musunuru division of cardiology and cardiovascular Institute Department of MedicineDepartment of GeneticsPerelman School of Medicine at the University of PennsylvaniaPhiladelphiaPA 19104USA

Genome editing has undergone rapid development in recent years,yielding new approaches to make precise changes in *** this review,we discuss the development of various adenine and cytosine base-editing technologies,which share the ability to make specific base changes at specific sites in the *** also describe multiple applications of base editing in vitro and in ***,as a practical example,we demonstrate the use of a cytosine base editor and an adenine base editor in human cells to introduce and then correct a prevalent mutation responsible for hereditary tyrosinemia type 1.

关键词： base editing cRISPR genetics genome editing mutation