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Soil c.nser.ation Measur.s in r.infed Olive Or.0}ar.s in South-Easter. Spain: Impac.s of Plant Str.ps on Soil Water.Dynamic.

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Pedospher. 2009年第4期19卷 453-464页

作者： V. H. DUr.N ZUAZO c. r. rodríguez pleguezuelo L. Ar.OYO PANADEr. A. MAr.íNEZ r.YA J. r. Fr.Nc.A MAr.íNEZ B. c.r.1}LES rodríguez Instituto de Investigac.ón y For.ac.ón Agr.r.a y Pesquer. IFAPA Centro Camino de Purchil

Sloping and mountainous olive pr.duc.ion systems ar. widespr.ad, oc.upying lar.e par.s of the Mediter.anean landsc.pe pr.ne to water.er.sion. Soil er.sion, r.noff, and soil water.c.ntent patter.s over.a thr.e-year.per.od wer. monitor.d in er.sion plots on a mountainside with r.infed olive （Olea eur.paea c.. Pic.al） tr.es under. 1） non-tillage with bar.ey str.ps of 4 m width （BS）; 2） non-tillage with native vegetation str.ps of 4 m width （NVS）; and 3） non-tillage without plant str.ps （NT）. The er.sion plots, loc.ted in Lanjar.n （Gr.nada, south-easter. Spain）, on a 30% slope, wer. 192 m2 in ar.a. For.assessing soil water.dynamic. in r.al-time and near.c.ntinuous soil water.c.ntent measur.ments, multisensor.c.pac.tanc. pr.bes wer. installed in the middle of plant str.ps and beneath the olive tr.e at five soil depths （10, 20, 30, 50, and 100 c.）. The highest er.sion and r.noff r.tes wer. measur.d under.NT, with a mean of 17.3 Mg ha-1 year.1 and 140.0 mm year.1, r.spec.ively, over.the entir. study per.od. The BS and NVS with r.spec. to the NT r.duc.d er.sion by 71% and 59% and r.noff by 95% and 94%, r.spec.ively. In gener.l, gr.ater.available soil water.c.ntent was found under.BS than NVS and NT, espec.ally beneath the olive tr.e c.nopies. These r.sults suppor.ed the r.c.mmendation of non-tillage with bar.ey str.ps in or.er.to r.duc. er.sion and to pr.ser.e soil water.for.tr.es in tr.ditional mountainous olive-pr.duc.ng ar.as, wher. or.0}ar.s c.ver.vast tr.c.s of land.

关键词： far.ing systems native plants r.noff soil er.sion

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Diagnosis and management of bac.er.al infec.ions in dec.mpensated c.r.hosis

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Wor.d Jour.al of Hepatology 2013年第1期5卷 16-25页

作者： Mar.a pleguezuelo Jose Manuel Benitez Juan Jur.do Jose Luis Monter. Manuel De la Mata Liver.r.sear.0} Unit Reina Sofia University HospitalAvda Menendez Pidal s/n14004 CordobaSpain

Bac.er.al infec.ions ar. one of the most fr.quent c.mplic.tions in c.r.hosis and r.sult in high mor.ality *** with c.r.hosis have alter.d and impair.d immunity,whic. favour. bac.er.al *** of infec.ions ar. mor. fr.quent in patients with dec.mpensated c.r.hosis than those with c.mpensated liver.*** most c.mmon and life-thr.atening infec.ion in c.r.hosis is spontaneous bac.er.al per.tonitis followed by ur.nar. tr.c. infec.ions,pneumonia,endoc.r.itis and skin and soft-tissue *** with dec.mpensated c.r.hosis have inc.eased r.sk of developing sepsis,multiple or.an failur. and *** fac.or. assoc.ated with the development of infec.ions ar. sever. liver.failur.,var.c.al bleeding,low asc.tic.pr.tein level and pr.or.episodes of spontaneous bac.er.al per.tonitis (SBP).The pr.gnosis of these patients is c.osely r.lated to a pr.mpt and ac.ur.te *** appr.pr.ate tr.atment dec.eases the mor.ality *** str.tegies ar. the mainstay of the management of these *** antibiotic. should be star.ed immediately following the diagnosis of SBP and the fir.t-line antibiotic.tr.atment is thir.-gener.tion ***,the effic.c. of c.r.ently r.c.mmended empir.c.l antibiotic.ther.py is ver. low in nosoc.mial infec.ions inc.uding SBP,c.mpar.d to c.mmunity-ac.uir.d *** may be assoc.ated with the emer.enc. of infec.ions c.used by Enter.c.c.us faec.um and extended-spec.r.m β-lac.amasepr.duc.ng Enter.bac.er.ac.ae,whic. ar. r.sistant to the fir.t line antimic.obial agents used for.*** emer.enc. of r.sistant bac.er.a,under.ines the need to r.str.c. the use of pr.phylac.ic.antibiotic. to patients with the gr.atest r.sk of *** infec.ions should be tr.ated with wide spec.r.m *** studies of ear.y diagnosis,pr.vention and tr.atment ar. needed to impr.ve the outc.mes in patients with dec.mpensated c.r.hosis.

关键词： c.r.hosis Infec.ions Spontaneous bac.er.al per.tonitis Asc.tes Antibiotic.

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Pr.teomic.analysis for.developing new biomar.er. of hepatoc.llular.c.r.0}noma

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Wor.d Jour.al of Hepatology 2010年第3期2卷 127-135页

作者： Mar.a pleguezuelo Laur. M Lopez-Sanc.ez Antonio rodriguez-Ar.za Jose L Monter. Javier.Br.c.no r.ben c.r.a Jor.i Muntane Manuel de la Mata Liver.r.sear.0} Unit and Ac.demic.Depar.ment of Sur.er. Reina Sofia University Hospital r.sear.0} Unit Reina Sofia University Hospital Liver.r.sear.0} Unit and Ac.demic.Depar.ment of Sur.er. CIBERehd Reina Sofia University Hospital

AIM: To identify new mar.er. of hepatoc.llular.c.r.0}noma (Hc.) using a pr.teomic.analysis. METHODS: Patients with liver.c.r.hosis of the thr.e most fr.quent etiologies: hepatitis c.vir.s, hepatitis B vir.s and alc.holic.liver.disease, wer. inc.uded in the study. The samples wer. analysed by 2D-elec.r.phor.sis in or.er.to deter.ine the differ.ntial pr.tein expr.ssion. The pr.teins wer. separ.ted ac.or.ing to the c.ar.e in immobilized pH 3-10 gr.dient str.ps and then by sodium dodec.l sulfate polyac.ylamide gel elec.r.phor.sis. Pr.teins of inter.st wer. exc.sed, digested with tr.psin and the r.sulting peptides wer. separ.ted and identified. r.SULTS: Thr.e differ.ntially expr.ssed apolipopr.teins (Apo) wer. identified based on the pr.tein pr.file using pr.teomic.tec.niques: Apo-A1, Apo-A4 and Apo-E. Apo-A4 levels wer. signific.ntly lower.in Hc. than in non-Hc. patients r.gar.less of etiology (P r.ate logistic.r.gr.ssion showed that Apo-A4 and Apo-A1 wer. the only independent fac.or. r.lated to Hc. diagnosis (P r.c.iver.oper.ting c.ar.c.er.stic.(r.c. c.r.e inc.uding both Apo-A4 and Apo-A1 showed an ar.a under.the r.c.of 0.944 (P c.fic.ty of 0.81 for.diagnosis of Hc.. c.Nc.USION: Apo-A4 and Apo-A1 may be used c.inic.lly as biomar.er. of Hc. with a high sensibility and spec.fic.ty. These findings may pr.vide additional insights into the mec.anism of Hc. development and pr.gr.ssion.

关键词： Liver c.nc.r Apolipopr.teins Ser.m biomar.er. 2D polyac.ylamide gel elec.r.phor.sis Mass spec.r.metr.

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Str.tegies to r.duc. hepatitis c.vir.s r.c.r.enc. after.liver.tr.nsplantation

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Wor.d Jour.al of Hepatology 2013年第5期5卷 237-250页

作者： r.ben c.r.a Mar.a pleguezuelo Shir.n Elizabeth Khor.andi Diego Davila Abid Suddle Hec.or.Vilc.-Melendez Sebastian r.fian Manuel de la Mata Javier.Br.c.o Pedr. López c.ller. Nigel Heaton Institute of Liver.Studies King’s College HospitalLondon SE5 9RSUnited Kingdom Unit of Hepatology and Liver.Tr.nsplantation University Hospital Reina Sofías/n 1400 CórdobaSpain Unit of Hepatobiliar. Sur.er. and Liver.Tr.nsplantation University Hospital Reina Sofías/n 1400 CórdobaSpain

Hepatitis c.vir.s (Hc.) is a major.health pr.blem that leads to c.r.nic.hepatitis, c.r.hosis and hepatoc.llular.c.r.0}noma, being the most fr.quent indic.tion for.liver.tr.nsplantation in sever.l c.untr.es. Unfor.unately, Hc. r.-infec.s the liver.gr.ft almost invar.ably following r.per.usion, with an ac.eler.ted histor. of r.c.r.enc., leading to 10%-30% of patients pr.gr.ssing to c.r.hosis within 5 year. of tr.nsplantation. In this sense, some gr.ups have even advoc.ted for.not r.tr.nsplanting this patients, as lower.patient and gr.ftoutc.mes have been r.por.ed. However. the management of Hc. r.c.r.enc. is being optimized and sever.l str.tegies to r.duc. post-tr.nsplant r.c.r.enc. c.uld impr.ve outc.mes, dec.ease the r.te of r.-tr.nsplantation and optimize the use of available gr.fts. Thr.e moments may be the foc.s of potential ac.ions in or.er.to dec.ease the impac. of vir.l r.c.r.enc.: the pr.tr.nsplant moment, the tr.nsplant envir.nment and the post-tr.nsplant management. In the pr.-tr.nsplant setting, it is not well established if r.duc.ng the pr. tr.nsplant vir.l load affec.s the r.sk for.Hc. pr.gr.ssion after.tr.nsplant. Obviously, antivir.l tr.atment c.n r.nder.the patient Hc. r.A negative post tr.nsplant but the long-ter. benefit has not yet been fully established to justify the c.st and c.inic.l r.sk. In the tr.nsplant moment, fac.or. as donor.age, c.ld isc.emia time, gr.ft steatosis and isc.emia/r.per.usion injur. may lead to a higher.and mor. aggr.ssive vir.l r.c.r.enc.. After.the tr.nsplant, disc.ssion about immunosuppr.ssion and the moment to star. the tr.atment (pr.phylac.ic. pr.-emptive or.onc.-c.nfir.ed) together.with new antivir.l dr.gs ar. of inter.st. This r.view aims to help c.inic.ans have a global over.iew of posttr.nsplant Hc. r.c.r.enc. and str.tegies to r.duc. its impac. on our.patients.

关键词： Hepatitis c vir.s r.c.r.enc. Liver Tr.ns- plantation Outc.mes

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