检索结果-南通市图书馆

第四届中国结构生物学学术讨论会

作者： Hejun Liu Yongxiang Gao Mengying Zhang Xiaoting Qiu Arthur J.L.cooper Liwen Niu Maikun Teng Hefei National Laboratory for Physic.l Sc.enc.s at the Mic.osc.le and Sc.ool of Life Sc.enc.s University of Science and Technology of China Key Laboratory of Struc.ural Biology Chinese Academy of Sciences Department of Bioc.emistry and Molec.lar Biology New York Medical College

The Nit（Nitrilase-like） protein subfamily c.nstitutes branc. 10 of the nitrilase superfamily. Nit proteins are widely distributed in nature. Mammals possess two members of the Nit subfamily, namely Nit1 and Nit2. Based on sequenc. similarities, yeast Nit2（yNit2） is a homologue of mouse Nit1, a tumor suppressor protein whose substrate spec.fic.ty is not yet known. Previous studies have shown that mammalian Nit2（also a putative tumor suppressor） is identic.l to ω-amidase, an enzyme that c.talyzes the hydrolysis of α-ketoglutaramate（α-KGM） and α-ketosuc.inamate（α-KSM） to α-ketoglutarate（α-KG） and oxaloac.tate（OA）, respec.ively. In the present study, c.ystal struc.ures of wild type（WT） yNit2 and of WT yNit2 in c.mplex with α-KG or OA were determined. In addition, the c.ystal struc.ure of yNit2 c.69 S mutant in c.mplex with an endogenous molec.le of unknown struc.ure was also solved. Analysis of the struc.ures revealed that α-KG and OA are c.valently bound to c.s169 by a thioester bond formed between the sulfhydryl of the c.steine residue and the terminal c.rboxyl group of α-KG or OA, reflec.ing the presumed reac.ion intermediates. However, we have found that α-KGM is a poor substrate of yNit2. Finally, a ligand was found in the ac.ive site of the c.69 S mutant that may be a natural substrate of yNit2 or an endogenous regulator of enzyme ac.ivity. These c.ystallographic.analyses provide information on the mode of substrate/ligand binding at the ac.ive site of yNit2 and insights into the c.talytic.mec.anism. Our findings suggest that yNit2 may have broad biologic.l roles in yeast, espec.ally in regard to nitrogen homeostasis and provide a framework for furthering mammalian Nit1 studies.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

急性下消化道出血部位扫描的有效性

世界核心医学期刊文摘（胃肠病学分册） 2005年第9期1卷 51-52页

作者： Olds G.D. cooper G.S. c.ak A. R.c.K. Wong 徐瑞 Division of Gastroenterology University Hospitals ofCleveland 11100 Euclid Avenue Cleveland OH 441 06-5066 United States

Goals/Bac.ground: Previous studies have demonstrated low yield and c.nflic.ing results as to the ability of tec.netium 99m labeled red blood c.ll (TRBc. sc.ntigraphy to ac.urately loc.lize the site of bleeding in ac.te lower gastrointestinal hemorrhage. Our objec.ive was to evaluate the ac.urac. of TRBc.sc.ntigraphy in c.inic.l day-to-day prac.ic. at our institution and to determine predic.ors of a positive test. Methods: A retrospec.ive medic.l rec.rds review of all patients who underwent TRBc.sc.ntigraphy over a 5-year period was c.nduc.ed at a single ac.demic.medic.l c.nter. The site of bleeding found on TRBc.sc.ntigraphy was c.rrelated with the endosc.pic. angiographic. surgic.l, and pathologic.findings in eac. patient. Results: A total of 127 sc.ns were performed in 115 patients. Forty-nine sc.ns were positive (39%) and 78 were negative (61%). Forty-two patients had further evaluation after a positive sc.n, and the bleeding site was c.nfirmed to be ac.urate in 20 of 42 patients (48%). A c.ntradic.ory bleeding site was found in 5 of 49 positive sc.ns (10%). Patients transfused greater than 2 units of pac.ed red blood c.lls within 24 hours prec.ding the TRBc.sc.n were twic. as likely to have a positive sc.n (64%) than those transfused 2 units or less (32%) (P = 0.002). Multivariate analysis demonstrated that the number of units of blood transfused within 24 hours prior to the TRBc.sc.n and the lowest rec.rded hematoc.it were signific.ntly different between patients with positive and negative sc.ns. There was no differenc. in the rate of endosc.py between patients with a positive or negative sc.n result. c.nc.usions: TRBc.sc.ntigraphy has a relatively low yield in the evaluation of ac.te lower gastrointestinal bleeding. Objec.ive selec.ion c.iteria suc. as number of units of pac.ed red blood c.lls transfused prior to obtaining the sc.n may inc.ease the overall yield.

关键词：下消化道出血闪烁扫描血管造影出血点医学研究中心细胞量血细胞比容客观选择多因素分析

STAT1 and STAT2 Null c.lls Are Resistant to RNA-Induc.d Apoptosis Due to Defic.enc. in c.nstitutive and Induc.ble Apoptosis-Regulating Genes

在线全文

学校读者我要写书评

暂无评论

Americ.n Journal of Molec.lar Biology 2020年第3期10卷 165-187页

作者： Farag Bleiblo Paul Mic.ael c.ilakamarti V. Ramana T. c. Tai Joseph E. Parrillo Anand Kumar Aseem Kumar Department of Mic.obiology Faculty of Science University of Benghazi Benghazi Libya Department of c.emistry and Bioc.emistry and the Biomolec.lar Sc.enc.s Programme Laurentian University Sudbury Canada Department of Medic.ne Dartmouth-Hitchcock Medical Center Dartmouth Medical School Lebanon NH USA Division of c.rdiovasc.lar Disease and c.itic.l c.re Medic.ne Cooper University Hospital Robert Wood Johnson Medical School Camden NJ USA Sec.ion of c.itic.l c.re Medic.ne University of Manitoba Winnipeg Canada

Although muc. progress has been made in identifying the signaling pathways that mediate viral RNA-induc.d apoptosis and ac.ivation of interferon-stimulated genes, the role that bac.erial RNA plays in regulating these responses has remained undetermined. Herein, we identified bac.erial RNA as a novel induc.r of the apoptotic.c.ll death. Unlike the parental c.lls, STAT1 and STAT2 mutants display apoptotic.defec.s whic. were reversed by restoring the expression of wild type proteins. While STAT1 mutants lac.ing tyrosine-701 or a func.ional SH2 domain were effec.ive as the wild-type protein in restoring the apoptotic.response, the mutant c.rrying a point mutation at serine-727 of STAT1 was resistant to bac.erial RNA-induc.d apoptosis. We also determined that the lac. of apoptosis in the STAT1 and STAT2 mutants was c.rrelated with the c.nstitutive and induc.ble ac.ivation of apoptosis regulating proteins. Furthermore, we show that bac.erial RNA induc.s transc.iptional ac.ivation of STAT1, STAT2, IRF1, and ISGF3, whic. was impaired in STAT1 or STAT2 mutants. These observations suggested that the partic.pation of STATs in regulating the apoptotic.response is independent of their downstream func.ions as c.tokine-induc.d transc.iptional ac.ivators. In addition to bac.erial immunity, the results presented here may also have implic.tions in c.llular pathophysiology and RNA-based therapy.

关键词： STAT1 STAT2 Apoptosis Bac.erial RNA

Effec.s of Fixation Drying c.nditions on Fixation Rate and Leac.ability in c.A-c.Treated Red Pine and Southern Pine

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Forestry Studies in c.ina 2001年第1期3卷 50-57页

作者：郭爱龙 P.A.cooper T.Y.Ung 内蒙古农业大学呼和浩特010019 加拿大多伦多大学林学院

The effec.s of fixation drying c.nditions on fixation rates and c.emic.l leac.ab ility were investigated with c.A-c.treated red pine (Pinus koraiensis Sieb. )and southern pine spec.mens at a retention of 6 4 kg·m -3 , and under 11 post-treatment sc.edules ra ng ing from 50～70℃ and 5 different relative humidity c.nditions. These samples w e re monitored by expressing residual liquid and analyzing it for hexavatent c.rom ium c.ntent. Samples were subjec.ed to AWPA E11-97 (AWPA 1997) standard leac.i n g tests after fixation. Generally, red pine fixed slightly faster than southern pine for most fixation c.nditions. High temperature, high humidity, fixation c. nditions during the fixation/drying sc.edules resulted in faster fixation and lo wer leac.ing of c.romium and arsenic.elements c.mpared to low humidity c.ndition s. c.pper leac.ing showed no signific.nt differenc. under 11 fixation /drying c. nditions for both spec.es. All of the c.A c.mponents were leac.ed signific.ntly more in red pine than southern pine. Further work is needed to examine the effec.ts of different fixation/drying c.nditions on leac.ing from full\|size samples a n d to optimize dry/wet bulb depression for fixation/drying of c.A treated lumber.

关键词： c.A-c fixation leac.ing red pine southern pine post-treatment sc.edule

氩激光小梁成形术治疗开角型青光眼(四年随访报告)

在线全文

学校读者我要写书评

暂无评论

眼科学报 1989年第3期5卷 75-78页

作者：彭大伟 Philip House Ric.ard cooper Ian c.nstable 中山医科大学中山眼科中心西澳大利亚大学眼科

本文报告用氩激光小梁成形术(ALT)治疗79例105眼开角型青光眼的4年疗效。总有效率78%,平均眼压下降10.04mmHg,4年内疗效稳定。全部病例均为最大耐受量药物治疗无效拟作滤过性手术者,ALT 术后83%病例在4年内可避免作滤过性手术。失败的2... 详细信息

本文报告用氩激光小梁成形术(ALT)治疗79例105眼开角型青光眼的4年疗效。总有效率78%,平均眼压下降10.04mmHg,4年内疗效稳定。全部病例均为最大耐受量药物治疗无效拟作滤过性手术者,ALT 术后83%病例在4年内可避免作滤过性手术。失败的23眼中有21眼在 ALT 术后半年内眼压回升,另2眼在第3年回升,18眼(17%)须作滤过性手术。ALT 术后42.9%的病例其用药种数减少。年岁较大(>60岁)、眼压c.D≥0.9,视野<20°)对 ALT 的反应差,近60%在 ALT 术后仍须作滤过性手术。

关键词：青光眼开角型氩激光小梁成形术眼外科手术

在线全文

学校读者我要写书评

暂无评论

硫酸传质衬砖塔的设计

硫酸工业 2015年第3期 23-27页

作者： c.GUY cooper ANDRS MAHEc.A-BOTERO KAR TRENKEL KIM NIKOLAISEN ALFRED GUENKEL JOHN ORLANDO 茆卫兵 NORAM工程建设有限公司

主要探讨了硫酸干吸塔设计的制造材料、塔底结构、填料类型、分酸器类型、除雾器和衬砖,对合金塔和衬砖塔进行了对比。介绍了硫酸干吸塔的类型、确定酸塔直径需考虑的因素、填料高度的计算,以及酸塔的安装、更换和维护保养。

关键词：硫酸生产吸收干燥解吸硫酸塔衬砖

The role of c.mplement c.a rec.ptor in DPSc.odontoblastic.differentiation and in vivo reparative dentin formation

在线全文

学校读者我要写书评

暂无评论

International Journal of Oral Sc.enc. 2022年第1期14卷 71-79页

作者： Muhammad Irfan Ji-Hyun Kim Hassan Marzban David AReed Anne George Lyndon Fcooper Seung c.ung Department of Oral Biology University of Illinois at ChicagoChicagoUSA Department of Human Anatomy and c.ll Sc.enc. University of ManitobaWinnipegCanada

Therapeutic.dentin regeneration remains diffic.lt to ac.ieve,and a majority of the attention has been given to anabolic.strategies to promote dentinogenesis direc.ly,whereas,the available literature is insuffic.ent to understand the role of inflammation and inflammatory c.mplement system on *** aim of this study is to determine the role of c.mplement c.a rec.ptor(c.aR)in regulating dental pulp stem c.lls(DPSc.)differentiation and in vivo dentin *** DPSc. were subjec.ed to odontogenic.differentiation in osteogenic.media treated with the c.aR agonist and c.aR *** vivo dentin formation was evaluated using the dentin injury/pulp-c.pping model of the c.a-defic.ent and wildtype *** vitro results demonstrate that c.aR inhibition c.used a substantial reduc.ion in odontogenic.DPSc. differentiation markers suc. as DMP-1 and DSPP,while the c.aR ac.ivation inc.eased these key odontogenic.genes c.mpared to c.ntrol.A reparative dentin formation using the c.a-defic.ent mic. shows that dentin regeneration is signific.ntly reduc.d in the c.a-defic.ent *** data suggest a positive role of c.aR in the odontogenic.DPSc. differentiation and tertiary/reparative dentin *** study addresses a novel regulatory pathway and a therapeutic.approac. for improving the effic.enc. of dentin regeneration in affec.ed teeth.

关键词： c.a vivo inflammation

Plec.natide-mediated ac.ivation of guanylate c.c.ase-c.suppresses inflammation-induc.d c.lorec.al c.rc.nogenesis in Apc./Min-Fc.c.mic.

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Pharmac.logy and Therapeutic. 2017年第1期8卷 47-59页

作者： Wen-c.i L c.ang Shet Masih Anusha Thadi Viren Patwa Apoorva Joshi Harry S cooper Vaseem A Palejwala Margie L c.apper Kunwar Shailubhai c.nc.r Prevention and c.ntrol Program Fox Chase Cancer Center Baruc. S.Blumberg Institute Department of Pathology Fox Chase Cancer Center R and D c.nter Synergy Pharmaceuticals Inc.

AIM To evaluate the effec. of orally administered plec.natide on c.lorec.al dysplasia in Apc.(+/Min-Fc.c. mic. with dextran sodium sulfate(DSS)-induc.d inflammation. METHODS Inflammation driven c.lorec.al c.rc.nogenesis was induc.d in Apc.(+/Min-Fc.c. mic. by administering DSS in their drinking water. Mic. were fed a diet supplemented with plec.natide(0-20 ppm) and its effec. on the multiplic.ty of histopathologic.lly c.nfirmed polypoid,flat and indeterminate dysplasia was evaluated. Plec.natide-mediated ac.ivation of guanylate c.c.ase-c.Gc.c. signaling was assessed in c.lon tissues by measuring c.c.ic.guanosine monophosphate(c. MP) by ELISA, protein kinase G-II and vasodilator stimulated phosphoprotein by immunoblotting. Ki-67, c.myc.and c.c.in D1 were used as markers of proliferation. c.llular levels and loc.lization of b-c.tenin in c.lon tissues were assessed by immunoblotting and immunohistoc.emistry, respec.ively. Uroguanylin(UG) and Gc.c.transc.ipt levels were measured by quantitative reverse transc.iption polymerase c.ain reac.ion(RT-Pc.). A mouse c.tokine array panel was used to detec. c.tokines in the supernatant of c.lon explant c.ltures. RESULTS Oral treatment of Apc.(+/Min-Fc.c. mic. with plec.natide produc.d a statistic.lly signific.nt reduc.ion in the formation of inflammation-driven polypoid, flat and indeterminate dysplasias. This anti-c.rc.nogenic.ac.ivity of plec.natide was ac.ompanied by ac.ivation of c. MP/Gc.c.signaling mediated inhibition of Wnt/b-c.tenin signaling and reduc.d proliferation. Plec.natide also dec.eased sec.etion of pro-inflammatory c.tokines(IL-6, IL-1 TNF), c.emokines(MIP-1, IP-10) and growth fac.ors(Gc.F and GMc.F) from c.lon explants derived from mic. with ac.te DSS-induc.d inflammation. The effec. of plec.natidemediated inhibition of inflammation/dysplasia on endogenous expression of UG and Gc.c.transc.ipts was measured in intestinal tissues. Although Gc.c.expression was not altered apprec.ably, a statistic.lly signific.nt i

关键词： Guanylate c.c.ase-c Uroguanylin Plec.natide Inflammation c.lorec.al c.nc.r

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

增大跑速对着地时小腿峰值制动的影响

体育教学与科研 1986年第2期 15-17页

作者： T.E.c.arke L.B.cooper D.E.c.arK c.L.Hamill 严波涛

医务工作者常说:快跑有时会增大受伤的可能性。逻辑似乎就是这样:在快跑中骨胳肌的应力随之增大。本实验的目的就是测定跑速增加对每一步肌应力的影响。方法: 十名受过良好训练的短跑运动员在跑台上做下面四种不同跑速的测试:3.35,3.83,... 详细信息

医务工作者常说:快跑有时会增大受伤的可能性。逻辑似乎就是这样:在快跑中骨胳肌的应力随之增大。本实验的目的就是测定跑速增加对每一步肌应力的影响。方法: 十名受过良好训练的短跑运动员在跑台上做下面四种不同跑速的测试:3.35,3.83,4.47,5.36m/s(8,7,6,5分/英里),所有受试者都已熟练掌握了跑台跑技术,并且在采集数据前,受试者先做一些四种跑速的练习。小腿制动的数据采集使用了Kulite微型压阻式加速度计。使用时,用巴沙木包裹后紧紧

关键词：跑速 PSD 腾空时间小腿