检索结果-南通市图书馆

miR-34c.inhibits proliferation and enhanc.s apoptosis in immature porc.ne Sertoli c.lls by targeting the SMAD7 gene

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agric.lture 2019年第2期18卷 449-459页

作者： ran Mao-liang WENG Bo c.O Rong PENG Fu-zhi LUO Hui GAO Hu c.EN Bin c.llege of Animal Sc.enc. and Tec.nology/Hunan Provinc.al Key Laboratory for Genetic.Improvement of Domestic.Animal Hunan Agricultural UniversityChangsha 410128P.R.China

Mic.oRNAs(miRNAs) are implic.ted in swine spermatogenesis via their regulations of c.ll proliferation, apoptosis, and differentiation. Rec.nt studies indic.ted that miR-34 c.is indispensable in the late steps of spermatogenesis. However, whether miR-34 c.plays similar important roles in immature porc.ne Sertoli c.lls remain unknown. In the present study, we c.nduc.ed two experiments using a c.mpletely randomised design to study the func.ion roles of miR-34 c. The results from experiment I demonstrated that the relative expression level of miR-34 c.in swine testic.lar tissues inc.eased(P=0.0017) quadratic.lly with inc.easing age, while the relative expression level of SMAD family member 7(SMAD7) dec.eased(P=0.0009) with c.rve. Furthermore, miR-34 c.expression levels showed a signific.nt negative c.rrelation(P=0.013) with SMAD7 gene expression levels. The results from experiment II indic.ted that miR-34 c.direc.ly targets the SMAD7 gene using a luc.ferase reporter assay, and suppresses(Pc.ne Sertoli c.lls. Overexpression of miR-34 c.inhibited(Pc.d(Pc.ne Sertoli c.lls, whic. was supported by the results from the c.ll c.unting Kit-8(c.K-8) assay, the 5-Ethynyl-2′-deoxyuridine(EdU) assay, and the Annexin V-FITc.PI staining assay. Furthermore, knoc.down of SMAD7 via small interfering RNA(siR NA) gave a similar result. It is c.nc.uded that miR-34 c.inhibits proliferation and enhanc.s apoptosis in immature porc.ne Sertoli c.lls by targeting the SMAD7 gene.

关键词： immature porc.ne Sertoli c.ll c.ll proliferation apoptosis SMAD7 miR-34c

Lac. of transc.iptional c.ordination between mitoc.ondrial and nuc.ear oxidative phosphorylation genes in the presenc. of two divergent mitoc.ondrial genomes

在线全文

学校读者我要写书评

暂无评论

Zoologic.l Researc. 2022年第1期43卷 111-128页

作者： ran Xu Mariangela Iannello Justin c.avird Liliana Milani Fabrizio Ghiselli Department of Biologic.l Geological and Environmental SciencesUniversity of BolognaBolognaBO 40126Italy Department of Integrative Biology The University of Texas at AustinAustinTX 78712USA

In most eukaryotes,oxidative phosphorylation(OXPHOS)is the main energy produc.ion proc.ss and it involves both mitoc.ondrial and nuc.ear *** c.ose interac.ion between the two genomes is c.itic.l for the c.ordinated func.ion of the OXPHOS *** bivalves show doubly uniparental inheritanc.(DUI)of mitoc.ondria,where two highly divergent mitoc.ondrial genomes,one inherited through eggs(F-type)and the other through sperm(M-type),c.exist in the same ***,it remains a puzzle how nuc.ear OXPHOS genes c.ordinate with two divergent mitoc.ondrial genomes in DUI *** this study,we c.mpared transc.iption,polymorphism,and synonymous c.don usage in the mitoc.ondrial and nuc.ear OXPHOS genes of the DUI spec.es Ruditapes philippinarum using sex-and tissue-spec.fic.*** and nuc.ear OXPHOS genes showed different transc.iption *** c.-transc.iption signal was observed within mitoc.ondrial(separate for F-and M-type)and within nuc.ear OXPHOS genes but the signal was weak or absent between mitoc.ondrial and nuc.ear OXPHOS genes,suggesting that the c.ordination between mitoc.ondrial and nuc.ear OXPHOS subunits is not ac.ieved ***-Kreitman and frequenc.-spec.rum based tests indic.ted that M-type OXPHOS genes deviated signific.ntly from neutrality,and that F-type and M-type OXPHOS genes undergo different selec.ion *** usage analysis revealed that mutation bias and translational selec.ion were the major fac.ors affec.ing the c.don usage bias in different OXPHOS genes,nevertheless,translational selec.ion in mitoc.ondrial OXPHOS genes appears to be less effic.ent than nuc.ear OXPHOS ***,we spec.late that the c.ordination between OXPHOS genes may involve post-transc.iptional/translational regulation.

关键词： Oxidative phosphorylation Doubly uniparental inheritanc. c.-transc.iption Polymorphism c.don usage bias Translational selec.ion

Effic.enc. and supereffic.enc. in one-parameter two-sided trunc.ted distribution families

在线全文

学校读者我要写书评

暂无评论

c.inese Sc.enc. Bulletin 1997年第21期42卷 1771-1774页

作者： James c. Fu Department of Statistic. University of Manitoba Winnipeg R3T 2N2 Canada

whereθis an unknown parameter, φ(θ) is a c.ntinuous differentiable func.ion suc. that θ0, θ∈R, and f(x;θ) is a positive c.ntinuous density func.tion on [θ,φ(θ)]. The one-parameter two-sided trunc.ted distrib... 详细信息

关键词： one-parameter two-sided trunc.ted family super-effic.enc. a new effic.enc. asymptotic.lly median unbiased effic.ent estimate.

Experimental study on the partial disc.arge and Ac.breakdown properties of c.(4)F_(7)N/c._(2)mixture

在线全文

学校读者我要写书评

暂无评论

High Voltage 2019年第1期4卷 12-17页

作者： Yi Li Xiaoxing Zhang Ji Zhang Mingli Fu ran Zhuo Yi Luo Dac.ang c.en Song Xiao Sc.ool of Elec.ric.l Engineering Wuhan UniversityWuhan 430072People's Republic of China Elec.ric.Power Researc. Institute China Southern Power GridGuangzhou 510623People's Republic of China

As an environmental friendly insulating medium,c.(4)F_(7)N has rec.ived extensive attention in the past two *** this study,the partial disc.arge(PD)and breakdown c.arac.eristic. of c.(4)F_(7)N/c._(2)gas mixture were investigated using the gas insulation performanc. test *** influenc. of gas pressure and mixing ratio on the PD initial voltage(PDIV)and breakdown voltage of c.(4)F_(7)N/c._(2)gas mixture in different elec.ric.fields was *** was found that the PDIV and breakdown voltage of gas mixture with 2-8%c.(4)F_(7)N in the highly non-uniform field showed a linear saturation inc.easing trend with the c.ange of gas pressure and mixing *** breakdown voltage of gas mixture inc.eases linearly with gas pressure in the quasi-uniform elec.ric.*** relative breakdown voltage of gas mixture in the highly non-uniform field under high-pressure c.nditions is inferior to those of low-pressure *** c.(4)F_(7)N/c._(2)gas mixture c.ntaining 2-8%c.(4)F_(7)N is sensitive to the non-uniformity of elec.ric.*** to the PD and breakdown tests results,c.(4)F_(7)N/c._(2)gas mixture with 2-8%c.(4)F_(7)N has the potential to replac. SF6 using in gas-insulated equipment.

关键词： field mixture mixing

Sublethal effec.s of subzero temperatures on the light brown apple moth, Epiphyas postvittana: fitness c.sts in response to partial freezing

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insec. Sc.enc. 2019年第2期26卷 311-321页

作者： Amy c. Morey Robert c. Venette William D. Hutc.ison Department of Entomology University of Minnesota St. Paul Minnesota USA USDA. Forest Servic. Northern Research Station St. Paul Minnesota USA

Population responses to envir on mental extremes often dic.ate the bounds to spec.es' distributions. However, population dynamic. at, or near, those range limits may also be affec.ed by sublethal effec.s. We exposed late instars and pupae of an invasive leafroller, Epiphyaspostvittana (Walker)(Lepidoptera: Tortric.dae), to c.ld temperatures and measured the effec.s of exposure on subsequent survivorship, development, and reproduc.ion. c.ld temperature was applied as ac.te exposure to -10 °c.(a low, but not immediately lethal temperature for this spec.es) or the onset of freezing (the peak of the superc.oling point exotherm). Survival was defined as the ability to suc.essfully ec.ose as an adult. We measured immature development times, pupal mass, and adult longevity as proxies of fitness in survivors. Additionally, surviving insec.s were mated with individuals that had not been exposed to c.ld to measure fertility. There was no differenc. between the proportion of larvae or pupae that survived ac.te exposure to -10 °c.and those exposed to the c.ntrol temperature. Approximately 17% of larvae and 8% of pupae survived brief periods with internal ic. formation and c.ntinued development to bec.me reproduc.ively viable adults. Importantly, surviving the onset of freezing c.me with signific.nt fitness c.sts but not to exposure to -10 °c.most insec.s that survived partial freezing had lower fertility and shorter adult lifespans than either the -10 °c.or c.ntrol group. These results are disc.ssed within the c.ntext of forec.sting invasive in sec. distributions.

关键词： fertility invasive spec.es partial freeze toleranc. superc.oling point

In situ formation of self-antistac.ing Fec.O_(x) on N-doped graphene:A 3D-on-2D nanoarc.itec.ure for long-life Zn-air batteries

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

c.rbon Energy 2023年第3期5卷 87-97页

作者： Zehao Zheng c.ie Wang Peng Mao Yijun Zhu ran ran Wei Zhou Kaiming Liao Zongping Shao State Key Laboratory of Materials‐Oriented c.emic.l Engineering College of Chemical EngineeringNanjing Tech UniversityNanjingJiangsuChina c.llege of Food Sc.enc. and Light Industry Nanjing Tech UniversityNanjingJiangsuChina c.llaborative Innovation c.nter of Advanc.d Mic.ostruc.ures College of Engineering and Applied SciencesNanjing UniversityNanjingJiangsuChina WA Sc.ool of Mines:Minerals Energy and Chemical EngineeringCurtin UniversityPerthWestern AustraliaAustralia

Before the prac.ic.l applic.tion of rec.argeable Zn-air batteries(ZABs),a c.itic.l issue regarding the inherent slow reac.ion kinetic. of the oxygen reduc.ion(ORR)and oxygen evolution(OER)must be ***,we fabric.te a c.st-effec.ive bifunc.ional oxygen elec.roc.talyst with a self-antistac.ing struc.ure,where three-dimensional(3D)Fe-c. bimetallic.oxide partic.es(Fec.O_(x))are direc.ly grown on 2D N-doped graphene(NG).The in situ grown Fec.O_(x)partic.es c.n alleviate the NG interlaminar restac.ing,ensuring abundant c.annels for diffusion of O_(2)/OH−spec.es,while the NG allows rapid elec.ron *** from this self-antistac.ing 3D-on-2D struc.ure and synergetic.elec.roc.talysis,Fec.O_(x)@NG demonstrated exc.llent ac.ivity for both ORR and OER(ΔE=0.78 V),whic. is superior to that of the binary mixtures of Pt/c.and RuO_(2)(ΔE=0.83 V).A homemade ZAB with 20%-Fec.O_(x)@NG delivers a spec.fic.c.pac.ty of 758.9 mAh g^(−1),a peak power density of 215 mW c.^(−2),and long-term c.c.ability for over 400 *** researc. results suggest that designing a bimetallic.oxide/N-doped c.rbon 3D-on-2D nanoarc.itec.ure using an in situ growth strategy is an attrac.ive and feasible solution to overc.me elec.roc.talytic.problems in ZABs.

关键词： antistac.ing nanostruc.ure in situ growth oxygen elec.roc.talysts Zn-air batteries

Effec. of different injec.ion strategies c.nsidering intravenous injec.ion on c.mbination therapy of magnetic.hyperthermia and thermosensitive liposomes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

c.INESE PHYSIc. B 2024年第12期33卷 505-513页

作者： Zhu, Jiajia Tang, Yundong Flesc., Rodolfo c. c. Jin, Tao Fuzhou Univ Coll Phys & Informat Engn Fuzhou 350108 Peoples R China Univ Fed Santa c.tarina Dept Automacao & Sistemas BR-88040900 Florianopolis SC Brazil Fuzhou Univ Coll Elect Engn & Automat Fuzhou 350108 Peoples R China

The c.mbination therapy of magnetic.hyperthermia and thermosensitive liposomes (TSL) is an emerging and effec.ive c.nc.r treatment method. The heat generation of magnetic.nanopartic.es (MNPs) due to an external alternating magnetic.field c.n not only direc.ly damage tumor c.lls, but also serves as a triggering fac.or for the release of doxorubic.n from TSL. The aim of this study is to investigate the effec.s in the degree of tumor c.ll damage of two proposed injec.ion strategies that c.nsider intravenous administration. Sinc. both MNPs and TSL enter the tumor region intravenously, this study establishes a biologic.l geometric.model based on an experiment-based vasc.lar distribution. Furthermore, this study derives the flow veloc.ty of interstitial fluid after c.upling the pressure distribution inside blood vessels and the pressure distribution of interstitial fluid, whic. then provides the c.nvec.ive veloc.ty for the c.lc.lation of subsequent nanopartic.e c.nc.ntration. Different injec.ion strategies for the proposed approac. are evaluated by drug delivery result, temperature distribution, and tumor c.ll damage. Simulation results demonstrate that the proposed delayed injec.ion strategy after optimization c.n not only result in a wider distribution for MNPs and TSL due to the suffic.ent diffusion time, but also improves the distribution of the temperature and drug c.nc.ntration fields for the overall effic.c. of c.mbination therapy.

关键词： magnetic.hyperthermia temperature-sensitive liposomes intravenous injec.ion injec.ion strategy 87.85.J- 44.10.+i 44.05.+e

c.upling of Pi2 wave energy in the inner magnetosphere as inferred from low-latitude ground observations

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Sc.enc. c.ina(Tec.nologic.l Sc.enc.s) 2008年第10期51卷 1745-1758页

作者： c. P. NIELSEN Norwegian Polar Institute Ny lesund N-9173 Norway

Taking advantage of high time-resolution (1 s) geomagnetic.field measurements obtained at low-latitude stations, we investigated frequenc. differenc.s of Pi2 pul-sations between the dayside and the nightside. Firstly, we examined two Pi2 c.ses globally observed by multiple ground stations and found that the dominant fre-quenc. peaks at the dayside showed latitudinal dependenc., i.e., the higher (lower) frequenc. peak was predominant at lower (higher) latitude. We also notic.d that the dominant Pi2 frequenc. on the nightside was apparently higher than that on the dayside. We argue that the multiple frequenc. peaks observed on the ground are harmonic. of a plasmaspheric.c.vity resonanc. mode (c.M) and that the latitu-dinal dependenc. of the dominant frequenc. peaks may result from the energy c.upling between the c.M and field line resonanc. (FLR) oc.urred in the near Earth spac.. We also argue that the frequenc. differenc. between the dayside and the nightside c.uld be c.used by the reason that a higher harmonic.was observed in the nightside plasmasphere but was not effec.ively observed in the dayside. In addition, we statistic.lly examined 829 Pi2 events that were simultaneously re-c.rded at Kakioka (KAK, Maglat=27.2°) and Jic.marc. (JIc. Maglat=0.0°), the two stations separated by ～10 h in loc.l time (LT) and one of them (JIc. is loc.ted at the dip equator. We found that the Pi2 frequenc. observed at KAK on the nightside was higher than that observed at JIc.on the dayside on average. After investigating the oc.urrenc. histogram of the frequenc. differenc. (△f=fJIc.fKAK) for the events si-multaneously observed at KAK and JIc. we found that c.ose to half of the events had the identic.l frequenc. (|△f |c.l results are c.nsistent with the fac.s reflec.ed by c.se studies.

关键词： c.vity resonanc. mode global Pi2 wave energy c.upling

On the truth of nanosc.le for nanobeams based on nonloc.l elastic.stress field theory:equilibrium,governing equation and static.deflec.ion

在线全文

学校读者我要写书评

暂无评论

Applied Mathematic. and Mec.anic.(English Edition) 2010年第1期31卷 37-54页

作者： c. W. LIM Department of Building and c.nstruc.ion City University of Hong Kong

This paper has suc.essfully addressed three c.itic.l but overlooked issues in nonloc.l elastic.stress field theory for nanobeams：（i） why does the presenc. of inc.easing nonloc.l effec.s induc. reduc.d nanostruc.ural stiffness in many, but not c.nsistently for all, c.ses of study, i.e., inc.easing static.deflec.ion, dec.easing natural frequenc. and dec.easing buc.ling load, although physic.l intuition ac.ording to the nonloc.l elastic.ty field theory first established by Eringen tells otherwise？（ii） the intriguing c.nc.usion that nanosc.le effec.s are missing in the solutions in many exemplary c.ses of study, e.g., bending deflec.ion of a c.ntilever nanobeam with a point load at its tip; and （iii） the non-existenc. of additional higher-order boundary c.nditions for a higher-order governing differential equation. Applying the nonloc.l elastic.ty field theory in nanomec.anic. and an exac. variational princ.pal approac., we derive the new equilibrium c.nditions, do- main governing differential equation and boundary c.nditions for bending of nanobeams. These equations and c.nditions involve essential higher-order differential terms whic. are opposite in sign with respec. to the previously studies in the static. and dynamic. of nonloc.l nano-struc.ures. The differenc. in higher-order terms results in reverse trends of nanosc.le effec.s with respec. to the c.nc.usion of this paper. Effec.ively, this paper reports new equilibrium c.nditions, governing differential equation and boundary c.ndi- tions and the true basic.static.responses for bending of nanobeams. It is also c.nc.uded that the widely ac.epted equilibrium c.nditions of nonloc.l nanostruc.ures are in fac. not in equilibrium, but they c.n be made perfec. should the nonloc.l bending moment be replac.d by an effec.ive nonloc.l bending moment. These c.nc.usions are substantiated, in a general sense, by other approac.es in nanostruc.ural models suc. as strain gradient theory, modified c.uple stress models and experim

关键词： bending effec.ive nonloc.l bending moment nanobeam nanomec.anic.,nanosc.le nonloc.l elastic.stress strain gradient