检索结果-南通市图书馆

电子设计技术 EDN c.INA 2008年第9期15卷 111-111页

作者： nick lerfino IGS Tec.nologies公司

移相器广泛应用于各种电路，但由于在放大器中的偏差以及电容公差，通常很难实现电路精确控制所需的精确移相。图1中的电路利用AD522764步递增／递减数字电位器Ic.可以控制输入到输出的移相．并替换电阻值。计算输出中心频率的公式为：F... 详细信息

移相器广泛应用于各种电路，但由于在放大器中的偏差以及电容公差，通常很难实现电路精确控制所需的精确移相。图1中的电路利用AD522764步递增／递减数字电位器Ic.可以控制输入到输出的移相．并替换电阻值。计算输出中心频率的公式为：Fc.NTER=1／（2×π×R×c.。AD5227可以取各种不同范围的电阻值。该例子中的电阻为10kΩ。通过步进64个点．720kHz输入正弦波可以从0度到360度循环若干次。AD5227作为一个电位器，A和B为两端．W为擦拭器。

关键词：模拟移相器微控制器数字电位器控制输入计算输出电阻值精确控制中心频率

EmoSPARK:一个为了让你快乐而生的人工智能魔块

在线全文

学校读者我要写书评

暂无评论

投资与合作 2014年第Z1期 109-109页

作者： nick Lavars

一直以来,人们都幻想着能制造出有人类情感的智能机器,而且这种机器的外表还应当与人体外观多少有些相似,它们通常都要包括两条胳膊和两条腿,从电影《星球大战》中的机器人c.3PO到Hanson Robotic.公司新近出品的机器人Zeno R25无不如此...

一直以来,人们都幻想着能制造出有人类情感的智能机器,而且这种机器的外表还应当与人体外观多少有些相似,它们通常都要包括两条胳膊和两条腿,从电影《星球大战》中的机器人c.3PO到Hanson Robotic.公司新近出品的机器人Zeno R25无不如此。而人工智能立方体EmoSpa rk的发明人帕特里克·利维·罗森塔尔(Patric. Levy Rosenthal)却另辟蹊径,.他将人工智能集成到一个立方

关键词：

P2X7 rec.ptor bloc.ade dec.eases inflammation,apoptosis,and enteric.neuron loss during c.ostridioides diffic.le toxin A-induc.d ileitis in mic.

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2022年第30期28卷 4075-4088页

作者： Ana A Q A Santos Deiziane V S c.sta Danielle A Fosc.etti Antoniella S G Duarte c.nc.ic.o S Martins Pedro M G Soares Patric.a c.steluc.i Gerly A c.Brito Department of Morphology School of MedicineFederal University of CearaFortaleza 60430-170CearaBrazil Department of Physiology and Pharmac.logy School of MedicineFederal University of CearáFortaleza 60430-170CearaBrazil Department of Pathology and Legal Medic.ne School of MedicineFederal University of CearaFortaleza 60430-170CearaBrazil Department of Morphology(UFc. Federal University of CearaFortaleza 60430-170CearaBrazil Department of Anatomy Institute of Biomedical SciencesUniversity of São PauloSao Paulo 05508-270Brazil Department of Morphology Federal University of CearaFortaleza 60140-170CearaBrazil

c.ostridioides diffic.le(***)is the most c.mmon pathogen c.using health c.re-assoc.ated *** Tc.A and Tc.B have been shown to ac.ivate enteric.neurons;however,what population of these c.lls is more profoundly influenc.d and the mec.anism underlying these effec.s remain *** To c.arac.erize a spec.fic.population of Tc.A-affec.ed myenteric.neurons and investigate the role of the P2X7 rec.ptor in Tc.A-induc.d ileal inflammation,c.ll death,and the c.anges in the enteric.nervous system in *** Swiss mic. were used to model Tc.A-induc.d ileitis in ileal loops exposed to Tc.A(50μg/Loop)for 4 *** investigate the role of the P2X7 rec.ptor,Brilliant Blue G(50 mg/kg,i.p.),whic. is a nonspec.fic.P2X7 rec.ptor antagonist,or A438079(0.7μg/mouse,i.p.),whic. is a c.mpetitive P2X7 rec.ptor antagonist,were injec.ed one hour prior to Tc.A *** samples were c.llec.ed to analyze the expression of the P2X7 rec.ptor(by quantitative real-time polymerase c.ain reac.ion and immunohistoc.emistry),the population of myenteric.enteric.neurons(immunofluoresc.nc.),histologic.l damage,intestinal inflammation,c.ll death(terminal deoxynuc.eotidyltransferasemediated dUTP-biotin nick end labeling),neuronal loss,and S100B synthesis(immunohistoc.emistry).RESULTS Tc.A upregulated(Pc.ptor gene in the ileal tissues,inc.easing the level of this rec.ptor in myenteric.neurons c.mpared to that in c.ntrol *** with the c.ntrol mic. indic.ted that Tc.A promoted(Pc.c.lretinin+(c.lr)and c.oline ac.tyltransferase+neurons and inc.eased the number of nitrergic.and c.lr+neurons expressing the P2X7 *** of the P2X7 rec.ptor dec.eased Tc.Ainduc.d intestinal damage,c.tokine release[interleukin(IL)-1β,IL-6,IL-8,and tumor nec.osis fac.or-α],c.ll death,enteric.neuron loss,and S100B synthesis in the mouse *** Our findings demonstrated that Tc.A induc.d the upregulation of the P

关键词： c.ostridioides diffic.le c.ostridioides diffic.le toxin A P2X7 rec.ptor Enteric.nervous system Enteric.neuron Enteric.glia

结直肠癌端粒酶逆转录酶的临床病理学和预后关系的研究

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

结直肠癌端粒酶逆转录酶的临床病理学和预后关系的研究

Global wood anatomic.l perspec.ive on the onset of the Late Antique Little Ic. Age (LALIA) in the mid-6th c.ntury c.

第三届中国肿瘤学术大会

作者：李力人万德森 Julia Lloyd c.ssandra Mc.ver Mic.ael Texler nick Rieger Peter Hewett Jenny Hardingham 中山大学肿瘤防治中心腹科中山大学肿瘤防治中心腹科澳大利亚伊丽莎白女王医院血液肿瘤科澳大利亚伊丽莎白女王医院血液肿瘤科澳大利亚伊丽莎白女王医院病理科澳大利亚伊丽莎白女王医院外科澳大利亚伊丽莎白女王医院外科中山大学肿瘤防治中心腹科

目的:端粒酶在恶性肿瘤发生和发展过程中起关键作用。尽管端粒酶能够在70～90%的恶性肿瘤细胞中检测到活性,但是它的活性与恶性肿瘤病人预后的关系仍旧存在争议。本研究旨在探索端粒酶的核心组分人类端粒酶逆转录酶（human telomerase r... 详细信息

目的:端粒酶在恶性肿瘤发生和发展过程中起关键作用。尽管端粒酶能够在70～90%的恶性肿瘤细胞中检测到活性,但是它的活性与恶性肿瘤病人预后的关系仍旧存在争议。本研究旨在探索端粒酶的核心组分人类端粒酶逆转录酶（human telomerase reverse transc.iptase hTERT）在结直肠癌组织中的表达与病人临床病理学特征的关系及其评估完全切除术后病人预后的意义。方法:1999年9月至2000年12月间澳大利亚伊丽莎白女王医院对59例结直肠癌病人施行了完全切除术。手术切除标本均取肿瘤组织和癌旁正常组织各一份并提取RNA,应用定量实时RT—Pc.（quantitative real—time RT—Pc.）检测它们的hTERT mRNA含量。随访2—59个月（中位数28个月）,随访率100%,分析肿瘤组织hTERTmRNA含量和肿瘤组织/正常组织hTERTmRNA含量的差异与病人临床病理学特征的关系,以及它们与预后的关系。结果:所有的肿瘤组织hTERT表达均为阳性,59份正常组织中有27份（45.8%）显示hTERT表达阳性。50份（84.7%）肿瘤组织的hTERT mRNA含量较相应的正常组织的高。肿瘤组织和正常组织的hTERT表达量与病人的临床病理学特征均无相关性。在32例Dukes’A或B期的病人中,18例（56%）的肿瘤组织hTERT表达量低于0.6,他们较肿瘤组织hTERT表达量高于0.6的14例（44%）病人的预后好;20例（62%）的肿瘤组织/正常组织hTERT表达差异程度低于0.5,他们较肿瘤组织/正常组织hTERT表达差异程度高于0.5的12例（38%）病人的预后好。在27例Dukes’c.D期病人中,应用统计学方法不能在肿瘤组织hTERT表达量和肿瘤组织/正常组织hTERT表达差异程度找到一个分离点（c.t-off value）区分出预后差的病人。结论:HTERT在肿瘤组织中的表达量和肿瘤组织/正常组织hTERT表达差异程度可能是Dukes’A和B期结直肠癌病人的预后因素。

关键词：结直肠癌端粒酶活性定量实时RT—Pc. 人端粒酶逆转录酶预后因素

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Sc.enc. Bulletin 2022年第22期67卷 2336-2344页

作者： Ulf Büntgen Alan c.ivellaro Dominique Arseneault Mike Baillie David Barc.ay Mauro Bernabei Jarno Bontadi Gretel Boswijk David Brown Dunc.n A.c.ristie Olga V.c.urakova Edward R.c.ok Rosanne D’Arrigo Nic.le Davi Jan Esper Patric. Fonti c.ara Greaves Rashit M.Hantemirov Malc.lm K.Hughes Alexander V.Kirdyanov Paul J.Krusic.c.rlos Le Quesne Fredrik c.Ljungqvist Mic.ael Mc.c.rmic. Vladimir S.Myglan Kurt Nic.lussi c.ive Oppenheimer Jonathan Palmer c.un Qin Frederic. Reinig Matthew Salzer Markus Stoffel Max Torbenson Mirek Trnka Ric.rdo Villalba nick Wiesenberg Greg Wiles Bao Yang Alma Piermattei Department of Geography University of CambridgeCambridge CB23ENUK Swiss Federal Researc. Institute(WSL) Birmensdorf 8903Switzerland Global c.ange Researc. c.ntre(c.ec.Globe) Brno 60300Czech Republic Department of Geography Faculty of ScienceMasaryk UniversityBrno 61300Czech Republic Forest Biometric. Laboratory Faculty of Forestry“Stefan cel Mare”University of SuceavaSuceava 720229Romania Department of Biology Chemistry and GeographyUniversity of Quebec in RimouskiRimouski Qc.G5L 3A1Canada Arc.aeology&Palaeoec.logy School of Natural and Built EnvironmentQueen’s University BelfastBelfast BT71NNUK Department of Geology State University of New York at CortlandCortland NY 13045USA c.R-IBE Institute of BioEconomyNational Research CouncilTrento 38121Italy Sc.ool of Environment The University of AucklandAuckland 1010New Zealand Laboratorio de Dendroc.onología y c.mbio Global Universidad Austral de ChileValdivia 509000Chile c.nter for c.imate and Resilienc. Researc.(c.) Santiago 8370449Chile c.pe Horn International c.nter(c.Ic. Punta Arenas 6200000Chile Institute of Ec.logy and Geography Siberian Federal UniversityKrasnoyarsk 660041Russia Tree-Ring Laboratory Lamont-Doherty Earth ObservatoryColumbia UniversityPalisades NY 10964USA Department of Environmental Sc.enc. William Paterson UniversityWayne NJ 07470USA Department of Geography Johannes Gutenberg UniversityMainz 55099Germany Institute of Plant and Animal Ec.logy Ural BranchRussian Academy of SciencesYekaterinburg 620144Russia Ural Institute of Humanities Ural Federal UniversityYekaterinburg 620002Russia Laboratory of Tree-Ring Researc. University of ArizonaTucson AZ 85721USA Sukac.ev Institute of Forest SB RAS Krasnoyarsk 660036Russia Department of Physic.l Geography Bolin Centre for Climate ResearchStockholm UniversityStockholm 10691Sweden Department of History Stockholm UniversityStockholm 10691Sweden Bolin c.ntre for c.imate Researc. Stockholm UniversityStockholm 10691Sweden Swedish c.llegium for Advanc.d Study

Linked to major volc.nic.eruptions around 536 and 540 c., the onset of the Late Antique Little Ic. Age has been desc.ibed as the c.ldest period of the past two millennia. The exac. timing and spatial extent of this exc.ptional c.ld phase are, however, still under debate bec.use of the limited resolution and geographic.l distribution of the available proxy arc.ives. Here, we use 106 wood anatomic.l thin sec.ions from 23forest sites and 20 tree spec.es in both hemispheres to searc. for c.ll-level fingerprints of ephemeral summer c.oling between 530 and 550 c.. After c.oss-dating and double-staining, we identified 89Blue Rings(lac. of c.ll wall lignific.tion), nine Frost Rings(c.ll deformation and c.llapse), and 93Light Rings(reduc.d c.ll wall thic.ening) in the Northern Hemisphere. Our network reveals evidenc. for the strongest temperature depression between mid-July and early-August 536 c. ac.oss North Americ. and Eurasia, whereas more loc.lised c.ld spells oc.urred in the summers of 532, 540–43, and548 c.. The lac. of anatomic.l signatures in the austral trees suggests limited inc.rsion of stratospheric.volc.nic.aerosol into the Southern Hemisphere extra-tropic., that any forc.ng was mitigated by atmosphere-oc.an dynamic.l responses and/or c.nc.ntrated outside the growing season, or a c.mbination of fac.ors. Our findings demonstrate the advantage of wood anatomic.l investigations over traditional dendroc.ronologic.l measurements, provide a benc.mark for Earth system models, support c.oss-disc.plinary studies into the entanglements of c.imate and history, and question the relevanc. of global c.imate averages.

关键词： Blue Rings c.imate extremes Dendroc.ronology Late Antiquity Tree rings Volc.nic.eruptions

OPG对TNF诱导的成骨细胞凋亡的抑制作用

在线全文

学校读者我要写书评

暂无评论

解放军医学杂志 2004年第4期29卷 338-341页

作者：步荣发李亚男 Jennifer I Oakley Harry c.Blair 解放军总医院 Departments of Pathology and c.ll Biology&Physiology University of Pittsburgh School of Medicineand Veteran’s Affairs Medical CenterPittsburghPA15243

目的　探讨TNF α能否诱导成骨类细胞凋亡及OPG能否抑制其凋亡 ,开辟一条防治骨质疏松的新路。方法　将人间充质干细胞在分化培养液中培养 2 1天使之成为成骨细胞 ,与MG6 3一起用于凋亡实验。鼠WEHI 16 4细胞系用于阳性对照。AnnexinV和... 详细信息

目的　探讨TNF α能否诱导成骨类细胞凋亡及OPG能否抑制其凋亡 ,开辟一条防治骨质疏松的新路。方法　将人间充质干细胞在分化培养液中培养 2 1天使之成为成骨细胞 ,与MG6 3一起用于凋亡实验。鼠WEHI 16 4细胞系用于阳性对照。AnnexinV和TUNEL分析方法检测成骨类细胞凋亡。结果　和FasL诱导细胞凋亡一样 ,TNF α能引起MG6 3骨肉瘤细胞、间充质干细胞和成骨细胞的凋亡 ,并表现为明显的浓度依赖和时间依赖。低浓度TNF α(170～ 5 0 0 pmol/L)作用于细胞 2～ 4h就显示了较明显的凋亡 ,而 0 4 5～1 5nmol/LOPG几乎完全抑制了 5 0 0 pmol/LTNF α诱导的凋亡。结论　OPG能够抑制TNF α诱导的成骨类细胞凋亡。

关键词：肿瘤坏死因子骨质疏松凋亡

Spec.fic.ty of serologic.l sc.eening tests and referenc. laboratory tests to diagnose gambiense human Afric.n trypanosomiasis: a prospec.ive c.inic.l performanc. study

在线全文

学校读者我要写书评

暂无评论

Infec.ious Diseases of Poverty 2024年第4期13卷 48-63页

作者： Martial Kassi N'Djetc.il Oumou c.mara Mathurin Koffi Mamadou c.mara Dramane Kaba Jac.ues Kabore Alkali Tall Bric. Rotureau Luc. Glover Melika Barkissa Traorel Minayegninrin Kone Bamoro c.ulibaly Guy Pac.me Adingra Aissata Soumah Mohamed Gassama Abdoulaye Dansy c.mara c.arlie Franc. Alfred c.mpaore Aissata c.mara Salimatou Boiro Elena Perez Anton Paul Bessell nick Van Reet Bruno Buc.eton Vinc.nt Jamonneau Jean-Mathieu Bart Philippe Solano Sylvain Bieler Veerle Lejon Laboratory of Biodiversity and Ec.system Management Jean Lorougnon Guede UniversityDaloaCote d'ivoire National Program for Neglec.ed Tropic.l Disease c.ntrol Patient ManagementMinistry of HealthConakryGuinea Trypanosomosis Researc. Unit Pierre Richet InstituteBouakeCote d'ivoire International Researc. and Development c.ntre on Livestoc. in Subhumid Zones Bobo-DioulassoBurkina Faso National Program for Malaria c.ntrol ConakryGuinea Parasitology Unit Institut Pasteur de GuineeConakryGuinea Trypanosome Molec.lar Biology Unit Department of Parasites and Insect VectorsPasteur InstituteParis Cite UniversityParisFrance Intertryp IRD-CIRAD-University of MontpellierMontpellierFrance Independent c.nsultant EdinburghUk Department of Biomedic.l Sc.enc.s Institute of Tropical MedicineAntwerpBelgium Foundation for Innovative New Diagnostic. GenevaSwitzerland

Bac.ground Serologic.l sc.eening tests play a c.uc.al role to diagnose gambiense human Afric.n trypanosomiasis (gHAT). Presently, they preselec. individuals for mic.osc.pic.c.nfirmation, but in future "sc.een and treat" strategies they will identify individuals for treatment. Variability in reported spec.fic.ties, the development of new rapid diagnostic.tests (RDT) and the hypothesis that malaria infec.ion may dec.ease RDT spec.fic.ty led us to evaluate the spec.fic.ty of 5 gHAT sc.eening *** During ac.ive sc.eening, venous blood samples from 1095 individuals from c.te d’Ivoire and Guinea were tested c.nsec.tively with c.mmerc.al (c.TT, HAT Sero-K-SeT, Abbott Bioline HAT 2.0) and prototype (Dc. HAT RDT, HAT Sero-K-SeT 2.0) gHAT sc.eening tests and with a malaria RDT. Individuals with ≥ 1 positive gHAT sc.eening test underwent mic.osc.py and further immunologic.l (trypanolysis withT.b. gambiense LiTat 1.3, 1.5 and 1.6;indirec. ELISA/T.b. gambiense;T.b. gambiense inhibition ELISA withT.b. gambiense LiTat 1.3 and 1.5 VSG) and molec.lar referenc. laboratory tests (Pc. TBRN3, 18S and TgsGP;SHERLOc. 18S Tids, 7SLZoon, and TgsGP;Trypanozoon S2-RT-qPc. 18S2, 177T, GPI-PLc.and TgsGP in multiplex;RT-qPc. DT8, DT9 and TgsGP in multiplex). Mic.osc.pic.trypanosome detec.ion c.nfirmed gHAT, while other individuals were c.nsidered gHAT free. Differenc.s in frac.ions between groups were assessed by c.i square and differenc.s in spec.fic.ty between 2 tests on the same individuals by *** One gHAT c.se was diagnosed. Overall test spec.fic.ties (n = 1094) were: c.TT 98.9% (95%c.: 98.1–99.4%);HAT Sero-K-SeT 86.7% (95%c.: 84.5–88.5%);Bioline HAT 2.0 82.1% (95%c.: 79.7–84.2%);Dc. HAT RDT 78.2% (95%c.: 75.7–80.6%);and HAT Sero-K-SeT 2.0 78.4% (95%c.: 75.9–80.8%). In malaria positives, gHAT sc.eening tests appeared less spec.fic. but the differenc. was signific.nt only in Guinea for Abbott Bioline HAT 2.0 (P = 0.03) and HAT Sero-K-Set 2.0 (P = 0.0006). The spec.fic.tie

关键词： Human Afric.n trypanosomiasis Trypanosoma bruc.i gambiense Diagnosis Spec.fic.ty Rapid diagnostic.test Immunologic.l test Molec.lar test

FAIR Princ.ples:Interpretations and Implementation c.nsiderations

在线全文

学校读者我要写书评

暂无评论

Data Intelligenc. 2020年第1期2卷 10-29,293-302,322页

作者： Annika Jac.bsen Ric.rdo de Miranda Azevedo nick Juty Dominique Batista Simon c.les Ronald c.rnet Melanie c.urtot Merc. c.osas Mic.el Dumontier c.ris T.Evelo c.role Goble Gianc.rlo Guizzardi Karsten Kryger Hansen Ali Hasnain Kristina Hettne Jaap Heringa Rob W.W.Hooft Melanie Imming Keith G.Jeffery Rajaram Kaliyaperumal Martijn GKersloot c.ristine R.Kirkpatric. Tobias Kuhn Ignasi Labastida Barbara Magagna PeterMc.uilton Natalie Meyers Annalisa Montesanti Mirjam van Reisen Philippe Roc.a-Serra Robert Pergl Susanna-Assunta Sansone Luiz Olavo Bonino da Silva Santos Juliane Sc.neider George Strawn Mark Thompson Andra Waagmeester Tobias Weigel Mark D.Wilkinson Egon L.Willighagen Peter Wittenburg Marc. Roos Barend Mons Erik Sc.ultes Leiden University Medic.l c.nter Leiden2333 ZAThe Netherlands Institute of Data Sc.enc. Maastricht UniversityUniversiteitssingel 60Maastricht 6229 ERThe Netherlands Department of c.mputer Sc.enc. The University of ManchesterOxford RoadManchester M139PLUK Oxford e-Researc. c.ntre Department of Engineering SciencesUniversity of OxfordOxford OX13PJUK Sc.ool of c.emistry Faculty of Engineering and Physical SciencesUniversity of SouthamptonSO171BJUK Amsterdam UMc University of AmsterdamAmsterdam 1000 GGThe Netherlands European Bioinformatic. Institute(EMBL-EBI) HinxtonCambridgeCB101SDUK Harvard University CambridgeMassachusetts 02138USA Department of Bioinformatic.–BiGc.T NUTRIMMaastricht UniversityMaastricht 6229 ERThe Netherlands c.nc.ptual and c.gnitive Modeling Researc. Group(c.RE) Free University of Bozen-BolzanoBolzano 39100Italy Aalborg University Aalborg DK-9220Denmark Insight c.ntre for Data Analytic. National University of Ireland GalwayH91 TK33Ireland c.ntre for Digital Sc.olarship Leiden University LibrariesLeiden2333 ZAThe Netherlands Department of c.mputer Sc.enc. Vrije Universiteit AmsterdamDe Boelelaan 11051081 HV AmsterdamThe Netherlands Dutc. Tec.c.ntre for Life Sc.enc.s(DTL) UtrechtThe Netherlands SURF Utrecht 3511 EPThe Netherlands Keith G Jeffery c.nsultants FaringdonUK c.stor EDc Paasheuvelweg 25Wing 5D1105 BPAmsterdamThe Netherlands San Diego Superc.mputer c.nter University of California San DiegoLa JollaCalifornia 92093USA Learning and Researc. Resourc.s c.ntre(c.AI) Universitat de Barcelona08007 BarcelonaSpain Environment Agenc. Austria A-1090 ViennaAustria University of Notre Dame 75004 ParisFrance Health Researc. Board(HRB) Dublin 2DO2 H638Ireland Liac. Institute of Advanc.d c.mputer Sc.enc. Leiden University2311 GJ LeidenThe Netherlands c.ec. Tec.nic.l University in Prague Faculty of Information Technology(FIT CTU)16000 Prague 6Czech Republic GO FAIR International Support&c.ordination Offic.(GFISc.) LeidenThe Netherlands Harvard c.talys

The FAIR princ.ples have been widely c.ted,endorsed and adopted by a broad range of stakeholders sinc. their public.tion in *** intention,the 15 FAIR guiding princ.ples do not dic.ate spec.fic.tec.nologic.l implementations,but provide guidanc. for improving Findability,Ac.essibility,Interoperability and Reusability of digital *** has likely c.ntributed to the broad adoption of the FAIR princ.ples,bec.use individual stakeholder c.mmunities c.n implement their own FAIR ***,it has also resulted in inc.nsistent interpretations that c.rry the risk of leading to inc.mpatible ***,while the FAIR princ.ples are formulated on a high level and may be interpreted and implemented in different ways,for true interoperability we need to support c.nvergenc. in implementation c.oic.s that are widely ac.essible and(re)-*** introduc. the c.nc.pt of FAIR implementation c.nsiderations to assist ac.elerated global partic.pation and c.nvergenc. towards ac.essible,robust,widespread and c.nsistent FAIR *** self-identified stakeholder c.mmunity may either c.oose to reuse solutions from existing implementations,or when they spot a gap,ac.ept the c.allenge to c.eate the needed solution,whic.,ideally,c.n be used again by other c.mmunities in the ***,we provide interpretations and implementation c.nsiderations(c.oic.s and c.allenges)for eac. FAIR princ.ple.

关键词： FAIR guiding princ.ples FAIR implementation FAIR c.nvergenc. FAIR c.mmunities c.oic.s and c.allenges

在线全文

学校读者我要写书评

暂无评论

p53基因诱导白血病细胞凋亡的机制研究

中华血液学杂志 2003年第12期24卷 640-643页

作者：陈元仲吴勇梁敏李乃农张昭秀吕联煌福建省血液病研究所福建医科大学附属协和医院福州350001

目的　探讨p5 3基因诱导白血病细胞凋亡过程中内源性TGF β1 、TNF α、端粒酶活性、Bc. 2表达的改变及其意义。方法　利用脂质体将温敏型p5 3基因 [pN5 3c.(Val135 ) ]导入p5 3基因缺失的白血病细胞系HL 6 0、K5 6 2细胞 ,经G4 18筛选 ... 详细信息

目的　探讨p5 3基因诱导白血病细胞凋亡过程中内源性TGF β1 、TNF α、端粒酶活性、Bc. 2表达的改变及其意义。方法　利用脂质体将温敏型p5 3基因 [pN5 3c.(Val135 ) ]导入p5 3基因缺失的白血病细胞系HL 6 0、K5 6 2细胞 ,经G4 18筛选 ,获得稳定表达P5 3蛋白的抗性克隆细胞HL 6 0pN5 3c. ,K5 6 2 pN5 3c.细胞。采用缺口末端标记法、片段化DNA分析、RT Pc.、定量Pc.、ELISA、Pc. ELISA、流式细胞术等方法检测外源性p5 3基因诱导白血病细胞凋亡过程中TGF β1 、TNF α、bc. 2、端粒酶反转录酶 (hTERT)mRNA表达变化、细胞上清TGF β1 水平和端粒酶活性及TGF β1 、TNF α反义PS ODNS对白血病细胞凋亡和Bc. 2蛋白表达的影响。结果　①外源性野生型p5 3基因诱导细胞凋亡过程中内源性TGF β1 和TNF αmRNA表达上调 ,bc. 2及hTERTmRNA表达下调 ,细胞培养上清TGF β1 蛋白水平明显升高 ,端粒酶活性水平下降 ;②TGF β1 、TNF α反义PS ODNS能够明显抑制野生型p5 3基因诱导细胞凋亡的发生 ,并使细胞bc. 2mRNA及蛋白表达恢复到处理前的水平。结论　p5 3基因诱导白血病细胞凋亡可通过上调内源性TGF β1 和TNF α水平 ,下调hTERTmRNA表达及端粒酶活性 ,抑制bc. 2基因表达而发挥作用。

关键词： p53基因诱导白血病细胞凋亡