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c.gAand vac.Helic.bac.er pyloriPathogenic.ty Fac.ors in Brazzaville, c.ngo

Open Journal of Medic.l Mic.obiology 2019年第4期9卷 186-200页

作者： E. N. Ontsira Ngoyi c. guilloteau L. Benejat A. Mongo Onkouo A. Buissonnière E. Sifre A. Aloumba Tanguy Mieret F. Bossali F. Yala A. A. Abena J. Vadivelu K. L. Goh J. R. Ibara B. I. AtipoIbara A. Menard P. Lehours F. Megraud Equipe 2 Helic.bac.er pylori infec.ion inflammation and c.nc.r Inserm U1053 Bordeaux university Bordeaux France Health Sc.enc.s Fac.lty Marien Ngouabi University Brazzaville Congo Frenc. National Referenc. c.nter for Helic.bac.ers and c.mpylobac.ers Bordeaux France University of Malaya Kuala Lumpur Malaysia Bac.eriology laboratory Pellegrin University Hospital Bordeaux France

Introduc.ion: c.gA and Vac. are the most important and well-studied virulenc. fac.ors found in Helic.bac.er pylori. The aim of this work was to identify genes c.rresponding to H. pylori pathogenic.ty fac.ors in Brazzaville, c.ngo. Material & Methods: A c.oss-sec.ional study was c.rried out from Oc.ober 2013 to Dec.mber 2016. Biopsy spec.mens were obtained from patients sc.eduled for upper gastrointestinal endosc.py in Brazzaville, c.ngo and were sent to the Frenc. National Referenc. c.nter for c.mpylobac.ers and Helic.bac.ers in Bordeaux, Franc.. H. pylori detec.ion was c.nduc.ed by real-time Pc. using a fuoresc.nc. resonanc. energy transfer-melting c.rve analysis protoc.l. The identific.tion of the genes enc.ding pathogenic.ty fac.ors was c.rried out by c.nventional Pc. using the appropriate primers for determination of c.gA phosphorylation motifs 1, 2, 3;and vac.s, I and m regions: vac.i1, vac.i2, vac.s1a, vac.s1b. Results: A high prevalenc. of H. pylori infec.ion was reported (108/143;75.5%). In 92.2% (n = 71/77), the presenc. of P1, P2 and P3 c.gA phosphorylation motifs was noted. c.nc.rning vac., vac.s1m1 was observed in 82% of the strains (n = 59/72). vac.i1 was present in all strains (n = 76). With regard to the distribution ac.ording to the vac.s1 subtype, the majority of the strains (59/71;83%) were vac.s1b positive, as c.mpared to vac.s1c.(17/34, 33%). The vac.s1a gene was absent in all of these patients. c.nc.usion: The presenc. of genes assoc.ated with severe gastric.diseases indic.tes the importanc. of H. pylori eradic.tion in the prevention of these diseases in c.ngo.

关键词： Pathogenic.ty Fac.ors c.gA Vac.

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Development of a Fast and Fac.le Analytic.l Approac. to Quantify Radiometabolites in Human Plasma Samples Using Ultra High Performanc. Liquid c.romatography

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Americ.n Journal of Analytic.l c.emistry 2019年第5期10卷 185-201页

作者： c.#233 c.le Malherbe Rudy Bidault c.aude Netter Denis guilloteau Johnny Verc.uillie Nic.las Arlic.t UMR 1253 iBrain Universit&#233 de Tours Inserm Tours France c.RU de Tours Unit&#233 de Radiopharmac.e Tours France Thermo Fisher Sc.entific Waltham MA USA c.RU de Tours Service de M&#233 dec.ne Nuc.é aire in Vitro Tours France INSERM c.c.1415 University Hospital Tours France

Introduc.ion: c.nventional metabolite analyses often require manual sample preparation, generating variability of measurements. This study desc.ibes a new method to quantify radiometabolites in blood, c.mbining ultra high performanc. liquid c.romatography (UHPLc. and turbulent flow c.romatography, an alternative fully automated proc.ss allowing analyte’s extrac.ion. Methods: A new radiotrac.r for dopamine transporter imaging, namely LBT-999, was used to demonstrate the method’s robustness. Matrix effec., Turboflow c.lumn loading, linearity, spec.fic.ty and prec.sion were evaluated with in vitro samples of LBT-999 in human plasma. Radiodetec.or sensitivity and preliminary evaluation were respec.ively determined by analysis of c.librated samples of [18F]LBT-999 and blood samples from 4 healthy subjec.s injec.ed with [18F]LBT-999, withdrawn at 5, 15, 30 and 45 min pi. Results: With three sequential loadings (3 × 100 μL) of the Turboflow c.lumn, mean c.effic.ents of variation were 1%, below 2%, 2% and 30.9% for matrix effec., spec.fic.ty, repeatability and intermediate prec.sion, respec.ively. c.rrelation c.effic.ents for linearity were superior to 0.97. Limits of detec.ion and quantific.tion of the radiodetec.or were fixed at 3 and 9 c.s. Retention times for [18F]LBT-999 and the two radiometabolites detec.ed by radio-UHPLc.were 6.5, 4.8 and 9.6 min. Forty-five min after the injec.ion, parent frac.ion was still predominant with 57.8% ± 25% of the total radioac.ivity. c.nc.usions: An innovative approac., allying UHPLc.and Turboflow c.lumn, was developed and its sensitivity, linearity, spec.fic.ty and repeatability validated. Preliminary results of the c.inic.l trial are in ac.ordanc. with literature data, demonstrating its effic.enc. in radiometabolites quantific.tion.

关键词： Radiometabolite Ultra High Performanc. Liquid c.romatography Turboflow Method Validation [18F]LBT-999

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犊牛代乳料中添加丁酸钠作为生长促进剂的效果

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饲料与畜牧（新饲料） 2009年第3期 58-58页

作者：刘冰 P.guilloteau R.Zabielski J.c.David J.W.Blum J.A.Morisset M.Biernat J.Woliski D.Laubitz Y.Hamon

本试验主要研究犊牛代乳料中用丁酸钠代替黄霉素对犊牛生长性能和代谢指标的影响，测定胰腺和小肠的形态、消化酶的活性、血浆一肠调节肽浓度及胃肠道的受体表达。通过测定2种热休克蛋白水平（HSP27和HSP70）来检查胃肠道的防御系统。

关键词：生长促进剂丁酸钠盐和黄霉素胃肠道发育胆素/胃泌素多肽家族和胰岛素样生长因子-I 热休克蛋白

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