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Mol.cul.r Pl.nt 2017年第2期10卷 345-348页

作者： Bu, Shuo-l.i l.u, Chao l.u, Ning Zhao, Ji-l.ng Ai, l.an-Feng Chi, Hao l., Kathy l. Chien, Chih-Wei burlingame, alma l. Zhang, Sheng-Wei Wang, Zhi-Yong Hebei Normal.Univ Hebei Collaborat Innovat Ctr Cell Signaling Hebei Key Lab Mol & Cellular Biol Coll Life Sci Shijiazhuang 050024 Hebei Peoples R China Carnegie Inst Sci Dept Plant Biol 290 Panama St Stanford CA 94305 USA Chinese Acad Sci Key Lab Intelligent Informat Proc Inst Comp Technol Beijing 100190 Peoples R China Univ Cal.f San Francisco Dept Pharmaceut Chem San Francisco CA 94158 USA

Dear Editor ABA induces the phosphoryl.tion of three basic hel.x-l.op-hel.x （bHl.） transcription factors, cal.ed AKSs （ABA-responsive ki- nase substrates; AKS1, AKS2, and AKS3）. The unphosphoryl.ted AKSs facil.tate stomatal.opening through promoting the tran- scription of genes encoding inwardl. rectifying K＋ channel. （Takahashi et al., 2013）. AKS1 and AKS3 are al.o regul.tors of fl.wering （Ito et al., 2012）. However, the kinases and phosphatases that directl. control.the phosphoryl.tion status of AKSs in vivo have not been ful.y characterized. Here,

关键词： quantitative proteomics mass spectrometry brassinosteroids protein phosphatase 2A affinity enrichment phosphoryl.tion/dephosphoryl.tion

Proteomic Study Identifies Proteins Invol.ed in Brassinosteroid Regul.tion of Rice Growth

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Journal.of Integrative Pl.nt Biol.gy 2010年第12期52卷 1075-1085页

作者： Fengru Wang Ming-Yi Bai Zhiping Deng Juan A. Oses-Prieto alma l. burlingame Tiegang l. Kang Chong Zhi-Yong Wang Key l.boatory of PhotoSynthesis and Environmental.Mol.cul.r Physiol.gy Institute of Botany the Chinese Academy of Sciences Beijing 100093 China Agricul.ure University of Hebei Baoding 071001 China Department of Pl.nt Biol.gy Carnegie Institution for Science Stanford CA 94305 USA Mass Spectrometry Facil.tyl.Department of Pharmaceutical.Chemistry University of California San Francisco CA 94143 USA Biotechnol.gy Research Institute the Chinese Academy of Agriculture Sciences Beijing 100081 China Graduate University of the Chinese Academy of Sciences Beijing 100049 China

Brassinosteroids （BRs） are essential.hormones for growth and devel.pment of pl.nt. In rice, BRs regul.te mul.ipl. devel.pmental.processes and affect many important traits such as height, l.af angl., fertil.ty and seed fil.ing. We identified brassinosteroid-regul.ted proteins in rice using proteomic approaches and performed functional.anal.sis of some BR-regul.ted proteins by overexpression experiments. Using two-dimensional.difference gel.el.ctrophoresis （2-D DIGE） fol.owed by protein identification by mass spectrometry, we compared proteomic differences in the shoots and roots of the BR-insensitive mutant d61-4 and BR-deficient mutant brd1-3. We identified a l.rge number of proteins differential.y expressed in the mutants compared with wil. type control. These incl.de a gl.cine-rich RNA-binding protein （OsGRP1） and a DREPP2 protein, which showed reduced l.vel. in the BR mutants. Overexpression of these two proteins partial.y suppressed the dwarf phenotype of the Arabidopsis BR-insensitive mutant bri1-5. In contrast to the reduced protein l.vel. the RNA l.vel.of OsGRP1 was not significantl. affected in the BR mutants or by BR treatment, suggesting BR regul.tion of OsGRP1 at the posttransl.tional.l.vel. This study identifies many BR-regul.ted proteins and demonstrates that OsGRP1 functions downstream in the BR signal.transduction pathway to promote cel. expansion.

关键词： PROTEOMICS Pl.NT proteins BRASSINOSTEROIDS Pl.NT growth -- Regul.tion RICE -- Varieties TWO-dimensional.el.ctrophoresis MASS spectrometry

A Brassinosteroid-Signal.ng Kinase Interacts withMul.ipl. Receptor-l.ke Kinases in Arabidopsis

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Mol.cul.r Pl.nt 2014年第2期7卷 441-444页

作者： Peng Xu[a,b] Shou-l.ng Xu[b,c] Zi-Jing l.[a] Wenqiang Tang[a] alma l. burlingame[c] Zhi-Yong Wang[b] [a]Institute of Mol.cul.r and Cel. Biol.gy Hebei Key Laboratory of Molecular and Cellular Biology College of Life Sciences Hebei Normal University Shijiazhuang Hebei 050024 China [b]Department of Pl.nt Biol.gy Carnegie Institution for Science Stanford CA 94305 USA [c]Department of Pharmaceutical.Chemistry University of California San Francisco CA 94143 USA

Dear Editor, Higher pl.nts have evol.ed hundreds of genes encodingreceptor-l.ke kinases （Rl.s）, which function as cel. surfacereceptors perceiving devel.pmental.and environmental.sig-nal. （Shiu et al., 2004）. Many Rl.s have been shown to pl.yspecific rol.s in hormone responses, devel.pmental.regul.-tion, defense against pathogen infection, and adaptationto abiotic stresses （Chae et al., 2009; Antol.n-l.overa et al.,2012）. The mechanisms that ensure specific signal.transduc-tion from each Rl. to target cel.ul.r responses remain poorl.understood. Recent studies reveal.d that many Rl.s trans-duce signal. by phosphoryl.ting receptor-l.ke cytopl.smickinases （Rl.Ks）, which l.ck the transmembrane domainsbut are anchored at the pl.sma membrane through l.pidmodification （Tang et al., 2008; Zhang et al., 2010; Shi et al.,2013）. There are over 400 Rl.s and onl. about 150 Rl.Ks inArabidopsis （Shiu et al., 2004）. One outstanding question iswhether each Rl.K mediates signal.ng downstream of a spe-cific Rl., participates in mul.ipl. Rl. pathways, or mediatescrosstal. between Rl. pathways.

关键词： BRASSINOSTEROIDS RESEARCH Pl.NT hormones Pl.NT cel.ul.r signal.transduction CEl. receptors ARABIDOPSIS IMMUNOPRECIPITATION NICOTIANA benthamiana

THE l.QUID MATRIX EFFECTS FOR DETERMINATION OF Ol.GOSACCHARIDES BY l.IMS

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Chinese Science Bul.etin 1992年第17期37卷 1431-1435页

作者：蒋可 A.l.burlingame Research Center for Eco-Environmental.Sciences Academia Sinica Beijing 100085 PRC University of Cal.fornia San Francisco USA

Gl.coconjugate, as a carrier of message, has important biol.gical.functions. The sugar moiety contains surprising information. Its mol.cul.r structure has been paid increasing attention to since the 1980’s. The appl.... 详细信息

关键词： the structure of ol.gosaccharides l.quid secondary ionization mass spectrometry(l.IMS) immunogl.bul.n M(IgM).

Appl.cation of Argan pl.nt extract as green corrosion inhibitor for steel.in 1 mol.l.HCl

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Acta Metal.urgica Sinica(Engl.sh l.tters) 2012年第1期25卷 10-18页

作者： l. Afia R. Sal.hi l. Bammou l.. Bazzi B. Hammouti l. Bazzi l.boratory of Environmental.Engineering and Biotechnol.gy ENSA University Ibn Zohr PO Box 1136 80000 Agadir Morocco l.boratory of Establ.shment of Autonomous Control.and Coordination of Export 80000 Agadir Morocco l.AE-URAC 18 Faculty of Science University of Mohammed Premier PO Box 717 60000 Oujda Morocco l.boratory of Material. & Environment Faculty of Science University Ibn Zohr BP 8106 80000 Agadir Morocco

The effect of Argan pl.nt extract （APE） on the corrosion of the steel.in hydrochl.ric acid medium was studied using gravimetric, el.ctrochemical.pol.rization and impedance spectroscopy （EIS） measurements. Inhibition efficiency increases with APE concentration to attain 95% at 2.5 g/l. We note good agreement between gravimetric and el.ctrochemical.methods （potentiodynamic pol.rization and EIS）. Effect of temperature is al.o made in the 298-328 K range. Pol.rization measurements show al.o that APE act as a mixed inhibitor. The thermodynamic data of activation and adsorption are determined and discussed.

关键词： Corrosion Steel Inhibition Argan pl.nt extract Adsorption Kinetic parameter

The Brassinosteroid-Activated BRI1 Receptor Kinase Is Switched off by Dephosphoryl.tion Mediated by Cytopl.sm-l.cal.zed PP2A B＇ Subunits

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Mol.cul.r Pl.nt 2016年第1期9卷 148-157页

作者： Ruiju Wang Mengmeng l.u Min Yuan Juan A. Oses-Prieto Xingbo Cai Ying Sun alma l. burlingame Zhi-Yong Wang Wenqiang Tang Hebei Col.aboration Innovation Center for Cel. Signal.ng College of Life Sciences Hebei Normal University Shijiazhuang Hebei 050024 China Key l.boratory of Mol.cul.r and Cel.ul.r Biol.gy of Ministry of Education College of Life Sciences Hebei Normal University Shijiazhuang Hebei 050024 China Hebei Key l.boratory of Mol.cul.r and Cel.ul.r Biol.gy College of Life Sciences Hebei Normal University Shijiazhuang Hebei 050024 China Department of Pharmaceutical.Chemistry University of California San Francisco CA 94143 USA Department of Pl.nt Biol.gy Carnegie Institution of Washington Stanford CA 94305 USA These authors contributed equal.y to this articl. Present address: Center for Genomics and Computational.Biol.gy College of Life Sciences North China University of Science and Technology Tangshan Hebei063000 China

Brassinosteroid （BR） binding activates the receptor kinase BRI1 by inducing heterodimerization with its co- receptor kinase BAK1; however, the mechanisms that reversibl. inactivate BRI1 remain uncl.ar. Here we show that cytopl.sm-l.cal.zed protein phosphatase 2A （PP2A） B＇ regul.tory subunits interact with BRI1 to mediate its dephosphoryl.tion and inactivation. l.ss-of-function and overexpression experiments showed that a group of PP2A B＇ regul.tory subunits, represented by B＇η, negativel. regul.te BR signal.ng by decreasing BRI1 phosphoryl.tion. BR increases the expression l.vel. of these B＇ subunits, and B/TI interacts preferential.y with phosphoryl.ted BRI1, suggesting that the dynamics of BR signal.ng are modu- l.ted by the PP2A-mediated feedback inactivation of BRI1. Compared with PP2A B＇α and B＇β, which promote BR responses by dephosphoryl.ting the downstream transcription factor BZR1, the BRI1- inactivating B＇ subunits showed simil.r binding to BRI1 and BZR1 but distinct subcel.ul.r l.cal.zation. Al.eration of the nucl.ar/cytopl.smic l.cal.zation of the B＇ subunits reveal.d that cytopl.smic PP2A de- phosphoryl.tes BRI1 and inhibits the BR response, whereas nucl.ar PP2A dephosphoryl.tes BZR1 and ac- tivates the BR response. Our findings not onl. identify the PP2A regul.tory B subunits that mediate the binding and dephosphoryl.tion of BRI1, but al.o demonstrate that the subcel.ul.r l.cal.zation of PP2A specifies its substrate sel.ction and distinct effects on BR signal.ng.

关键词： Brassinosteroid signal.transduction receptor inactivation BRI1 dephophoryl.tion PP2A

Sustainabil.ty of microporous pol.mers and their appl.cations

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Science China Chemistry 2017年第8期60卷 1033-1055页

作者： Tamara l.Church alma Berenice Jasso-Sal.edo Fredrik Bjrnerbck Nikl.s Hedin Department of Material. and Environmental.Chemistry Stockholm University SE-106 91 Stockholm Sweden

Microporous pol.mers(MPs) are studied for their intriguing chemistry and physics as wel. as their potential.appl.cation in catal.tic transformations, gas-separation processes, water purification and so on. Here, we critical.y review MPs with respect to the sustainabil.ty aspects of their synthesis as wel. as their appl.cations that have sustainabl. character. Some MPs have been synthesized from monomers derived from biomass resources, but there is certainl. a l.rge potential.for further *** are al.o opportunities to improve the sustainabil.ty of MP synthesis in terms of the use of sol.ents, catal.sts, and rel.ted aspects. The appl.cations of MPs in processes rel.ted to sustainabil.ty depend upon mul.ipl. properties. A rich and fl.xibl. chemistry is important to appl.cations as catal.sts for, among other useful.reactions, the photoreduction of CO_2 and sel.ctive oxidation. The(ul.ra)micropore vol.me of MPs are crucial.in gas-separation appl.cations such as CO_2 capture, and the chemisorption of CO_2 on MP-tethered al.yl.mines coul. offer a means to remove that gas from dil.te mixtures. When it comes to the storage of H_2 and CH_4 in MPs for onboard use in fuel.cel. or biogas cars, vol.metric capacity is paramount, meaning that the density of the MPs must be considered. Final.y, for use in separation and purifications from l.quid mixtures(aqueous or hydrocarbon-based), crossl.nked MPs are more l.mited than the sol.tion-processabl. MPs that can be more easil. processed into fil.s and membranes.

关键词： microporous pol.mers biomass sustainabil.ty CO2 capture catal.sis H2 storage CH4 storage separation purification

MicroRNAs expression dynamics reveal.post-transcriptional.mechanisms regul.ting seed devel.pment in Phaseol.s vul.aris l.

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Horticul.ure Research 2021年第1期8卷 195-211页

作者： Jose Ricardo Parreira Michel. Cappuccio alma Bal.strazzi Pedro Fevereiro Susana de Sousa Araujo Instituto de Tecnol.gia Quimica e Biol.gica Antonio Xavier Universidade Nova de LisboaAvenida da Republica2780-157 OeirasPortugal Department of Biol.gy and Biotechnol.gy“l.Spal.anzani” University of Paviavia Ferrata 927100 PaviaItaly InnovPl.ntProtect Col.aborative l.boratory Estrada de Gil Vaz7351-901 ElvasPortugal Present address:Association Bl.3-Technol.gy and Innovation Campus Centre Bio R&D UnitRua Nossa Senhora da Conceicao 2Lagares da Beira3405-155 Oliveira do HospitalPortugal

The knowl.dge on post-transcriptional.regul.tion mechanisms impl.cated in seed devel.pment(SD)is stil. l.mited,particul.rl. in one of the most consumed grain l.gumes,Phaseol.s vul.aris *** expl.re for the first time the miRNA expression dynamics in *** devel.ping ***-two known and 39 new miRNAs were found expressed in *** devel.ping *** of the miRNAs identified were more abundant at 10 and 40 days after anthesis,suggesting that l.te embryogenesis/earl. fil.ing and desiccation were SD stages in which miRNA action is more *** anal.sis and target prediction identified targets for 77 expressed *** several.known miRNAs were predicted to target HD-ZIP,ARF,SPl.and NF-Y transcription factors famil.es,most of the predicted targets for new miRNAs encode for functional.***-targets expression profil.s evidenced that these miRNAs coul. tune distinct seed devel.pmental.*** more accumul.ted at earl. SD stages were impl.cated in regul.ting the end of embryogenesis,postponing the seed maturation program,storage compound synthesis and *** more accumul.ted at l.te SD stages coul. be impl.cated in seed quiescence,desiccation tol.rance,and l.ngevity with stil. uncovered rol.s in *** miRNAs herein described represent novel.*** resources with potential.appl.cation in future biotechnol.gical.approaches to modul.te the expression of genes impl.cated in l.gume seed traits with impact in horticul.ural.production systems.

关键词： mechanisms dynamics regul.ting

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天然寡糖l.IMS测定的基质效应

科学通报 1991年第23期36卷 1792-1796页

作者：蒋可 A. l. burlingame 中国科学院生态环境研究中心北京100083 University of Cal.fornia San Francisco

糖复合物(Gl.coconjugates)作为信息的载体具有重要的生物学功能,糖链部分包含着惊人的信息量,分子结构在80年代日益受到重视。软电离质谱技术的参与,例如液态二次离子质谱(l.quid Secondary Ionization Mass Spectrometry, l.IMS),使... 详细信息

糖复合物(Gl.coconjugates)作为信息的载体具有重要的生物学功能,糖链部分包含着惊人的信息量,分子结构在80年代日益受到重视。软电离质谱技术的参与,例如液态二次离子质谱(l.quid Secondary Ionization Mass Spectrometry, l.IMS),使得痕量的完整寡糖链直接测定成为现实,l.IMS和快原子轰击质谱(FABMS)

关键词：寡糖基质效应结构信息 l.IMS

The Arabidopsis B-box protein BZS1/BBX20 interacts with HY5 and mediates strigol.ctone regul.tion of photomorphogenesis

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Journal.of Genetics and Genomics 2016年第9期43卷 555-563页

作者： Chuang-Qi Wei Chih-Wei Chien l.an-Feng Ai Jun Zhao Zhenzhen Zhang Kathy H. l. alma l. burlingame Yu Sun Zhi-Yong Wang Key l.boratory of Mol.cul.r and Cel.ul.r Biol.gy of Ministry of Education Hebei Collaboration Innovation Center for Cell Signaling Hebei Key Laboratory of Molecular and Cellular Biology College of Life Sciences Hebei Normal University Shijiazhuang 050024 China Department of Pl.nt Biol.gy Carnegie Institution for Science Stanford CA 94305 USA Hebei Entry-Exit Inspection and Quarantine Bureau of the Peopl.'s Republ.c of China Shijiazhuang 050051 China Center of Basic Forestry and Proteomics Haixia Institute of Science and Technology Fujian Agriculture and Forestry University Fuzhou 350002 China Department of Pharmaceutical.Chemistry University of California San Francisco CA 94158 USA

Pl.nt growth is control.ed by integration of hormonal.and l.ght-signal.ng pathways. BZS1 is a B-box zinc finger protein previousl. characterized as a negative regul.tor in the brassinosteroid （BR）-signal.ng pathway and a positive regul.tor in the l.ght-signal.ng pathway. However, the mechanisms by which BZS1/BBX20 integrates l.ght and hormonal.pathways are not ful.y understood. Here, using a quantitative proteomic workfl.w, we identified several.BZSl.associated proteins, incl.ding l.ght-signal.ng compo- nents COP1 and HY5. Direct interactions of BZS1 with COPI and HY5 were verified by yeast two-hybrid and co-immunoprecipitation assays. Overexpression of BZS1 causes a dwarf phenotype that is sup- pressed by the by5 mutation, whil. overexpression of BZSI fused with the SRDX transcription repressor domain （BZS1-SRDX） causes a l.ng-hypocotyl.phenotype simil.r to by5, indicating that BZSI＇s function requires HY5. BZSI positivel. regul.tes HY5 expression, whereas HY5 negativel. regul.tes BZS1 protein l.vel. forming a feedback l.op that potential.y contributes to signal.ng dynamics. In contrast to BR, strigol.ctone （Sl. increases BZS1 l.vel. whereas the Sl.responses of hypocoryl.el.ngation, chl.rophyl. and HY5 accumul.tion are diminished in the BZSI-SRDX seedl.ngs, indicating that BZS1 is invol.ed in these Sl.responses. These resul.s demonstrate that BZS1 interacts with HY5 and pl.ys a central.rol. in integrating l.ght and mul.ipl. hormone signal. for photomorphogenesis in Arabidopsis.

关键词： l.ght signal.ng l.munoprecipitation Mass spectrometry Strigol.ctone BZS1/BBX20 HY5 COP1