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Separated Hydrolysis and Fermentation of Water Hyacinth Leaves for Ethanol Production

Journal of Life Sciences 2011年第3期5卷 212-219页

作者： buddhiporn sornvoraweat jirasak kongkiattikajorn School of Bioresources and Technology King Mongkut＇s University of Technology Thonburi Bangkok 10150 Thailand

Water hyacinth is a raw material for long-term sustainable production of cellulosics ethanol. In this study, the acid pretreatment and enzymatic hydrolysis were used to evaluate to produce more sugar, to be fermented to ethanol. Separated hydrolysis and fermentation （SHF） studies were carried out to produce ethanol from water hyacinth leaves. Dilute sulfuric acid pretreatment and enzymatic hydrolysis were conducted to select the optimum pretreatment conditions. The optimum pretreatment conditions included T = 135 ℃, t = 30 min, and sulfuric acid concentration = 0.1 M. The residue was enzymatically hydrolyzed using the mixture of enzymes cellulase, xylanase and pectinase. The maximum enzymatic saccharification of cellulosic material （76.8%） was achieved. SHF by mono-culture of Saccharomyces cerevisiae KM1195 achieved the highest yields of ethanol. Furthermore, ethanol production was accomplished with the co-culture ofS. cerevisiae TISTR5048 and Candida tropicalis TISTR5045 which produced the highest increase in ethanol Yield. In this case, the ethanol concentration of 3.42 （g/L）, percentage of the theoretical ethanol yield of 99.9%, the ethanol yield of 0.27 g/g and the productivity of 0.22 g/L/h were obtained. This suggested that mild acid pretreatment and co-cultureare promising methods to improve enzymatic hydrolysis and ethanol production from water hyacinth.

关键词： Ethanol water hyacinth leaves fermentation.

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Bioproduction of Ethanol in Separate Hydrolysis and Fermentation and Simultaneous Saccharification and Fermentation from Cassava Stalks

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Journal of Food Science and Engineering 2012年第2期2卷 80-87页

作者： buddhiporn Sovorawet jirasak Kongkiattikajom School of Bioresources and Technology King Mongkut＇s University of Technology Thonburi Bangkok 10150 Thailand

Cellulose biomass is being investigated as a potential substrate for bioethanol production. Cassava stalks were successfully converted to ethanol by fermentation using Saccharomyces cerevisiae TISTR5048, S. cerevisiae KM1195, S. cerevisiae KM7253 and co-culture of S. cerevisiae TISTR5048 and Candida tropicalis TISTR5045. The objective of this study was to assess the ethanol production from cassava stalks by dilute-acid pretreatment and enzymatic hydrolysis that were convertible into ethanol by mono-culture and co-culture of yeast strain. Cassava stalks 1.5% （w/v） in 0.1 M sulfuric acid was pretreated for 30 min at 135 ℃ under the pressure of 15 lb/inch2. The pretreated cassava stalk suspensions were neutralized to pH 5.5 for saccharification process. The enzyme solution （a-amylase, amyloglucosidase, cellulase, xylanase and pectinase solubilized in buffer pH 5.0） was used for hydrolysis ofpretreated cassava stalk at 50 ℃ for 24 h. The hydrolyaste was supplemented with additional nutrients. The culture was incubated at 30 ℃. The pretreatment of the stalk with dilute-acid resulted sugar yield of 0.57 g/g dry matter from enzymatic hydrolysis, which was higher than dilute-alkaline-pretreated and distilled water-pretreated stalk. The sugar hydrolysate was bioconverted to ethanol with separate hydrolysis and fermentation （SHF） and simultaneous saccharification and fermentation （SSF）. The highest ethanol yields of 98.43% and 95.29% were obtained in SHF and SSF, respectively by S. cerevisiae KM1195. The fermentation time of SSF process was 24-32 h shorter than that of the SHF （= 56 h）, but not significantly leading to difference in ethanol production （5.42 g/L-6.22 g/L for SSF; 5.9 g/L-6.23 g/L for SHF）.

关键词： Ethanol cassava stalk fermentation.

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