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Identity and distribution of weedy Pedicularis kansuensis Maxim. (Orobanchaceae) in Tianshan Mountains of Xinjiang：morphological, anatomical and molecular evidence

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Journal of Arid Land 2016年第3期8卷 453-461页

作者： SUI Xiaolin Patrick kuss LI Wenjun YANG Meiqing GUAN Kaiyun LI Airong Yunnan Key Laboratory for Research and Development of Wild Plant Resources Kunming Institute of Botany Chinese Academy of Sciences Kunming 650201 China Institute of Systematic Botany University of Zurich Zurich 8008 Switzerland Key Laboratory of Biogeography and Bioresource in Arid Land Xinjiang Institute of Ecology and Geography Chinese Academy of Sciences Urumqi 830011 China University of Chinese Academy of Sciences Beijing 100049 China World Agroforestry Centre East and Central Asia Region Kunming 650201 China

Weedy plants affect the biodiversity and ecosystem function as well as the crop and fodder plant production. However, adequate management requires detailed knowledge of the taxonomic identity of these plants. Here, we focused on a hemiparasitic Pedicularis species（Orobanchaceae）, which occurs at high densities and results in significant biomass reductions in forage grasses in Bayanbulak Grassland of Xinjiang. The identity of this target species is not clear, with conflicting reports in publications and in herbarium collections. Hence, clear and management-relevant information on demography and reproductive ecology is difficult to be obtained from the literature. Therefore, we analyzed field and archival materials collected from Xinjiang in order to clarify the identity and distribution of the target species. Morphological analyses suggested that the populations at Bayanbulak Grassland should be Pedicularis kansuensis Maxim. rather than P. verticillata L. which has been accepted in the available literature. Phylogenetic analysis with a combination of three barcodes（mat K, rbc L and trn H-psb A） uniting a clade of P. kansuensis and individuals from Bayanbulak Grassland populations with 100% bootstrap support, confirmed the target species to be P. kansuensis. Anatomical investigations and field observations showed that the target species is an annual or biennial herb, which also fits with the life cycle as P. kansuensis. Based on archive material and field observations, we verified that the distribution of P. kansuensis is mainly concentrated in the Tianshan Mountains of Xinjiang.

关键词： DNA barcode herb-chronology morphological characters root hemiparasite

Anisotropic scaffolds for peripheral nerve and spinal cord regeneration

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Bioactive Materials 2021年第11期6卷 4141-4160页

作者： Wen Xue Wen Shi Yunfan Kong Mitchell kuss Bin Duan Mary&Dick Holland Regenerative Medicine Program University of Nebraska Medical CenterOmahaNEUSA Division of Cardiology Department of Internal MedicineUniversity of Nebraska Medical CenterOmahaNEUSA Department of Surgery University of Nebraska Medical CenterOmahaNEUSA Department of Mechanical Engineering University of Nebraska-LincolnLincolnNEUSA

The treatment of long-gap(>10 mm)peripheral nerve injury(PNI)and spinal cord injury(SCI)remains a continuous challenge due to limited native tissue regeneration *** current clinical strategy of using autografts for PNI suffers from a source shortage,while the pharmacological treatment for SCI presents dissatisfactory *** engineering,as an alternative,is a promising approach for regenerating peripheral nerves and spinal *** providing a beneficial environment,a scaffold is the primary element in tissue *** particular,scaffolds with anisotropic structures resembling the native extracellular matrix(ECM)can effectively guide neural outgrowth and *** this review,the anatomy of peripheral nerves and spinal cords,as well as current clinical treatments for PNI and SCI,is first *** overview of the critical components in peripheral nerve and spinal cord tissue engineering and the current status of regeneration approaches are also *** advances in the fabrication of anisotropic surface patterns,aligned fibrous substrates,and 3D hydrogel scaffolds,as well as their in vitro and in vivo effects are ***,we summarize potential mechanisms underlying the anisotropic architectures in orienting axonal and glial cell growth,along with their challenges and prospects.

关键词： Tissue engineering Topography Alignment Surface pattern Hydrogel

Spatial isolation and genetic differentiation in naturally fragmented plant populations of the Swiss Alps

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Journal of Plant Ecology 2008年第3期1卷 149-159页

作者： Patrick kuss AndreaRPluess Hafdı´s HannaÆgisdottir Jurg Stocklin Institute of Botany University of BaselSchonbeinstrasse 6CH-4056BaselSwitzerland Institute of Plant Sciences University of BernAltenbergrain21CH-3013BernSwitzerland Institute for Terrestrial Ecosystems Ecosystem ManagementETH ZurichUniversitatstrasse 168092 ZurichSwitzerland

The effect of anthropogenic landscape fragmentation on the genetic diversity and adaptive potential of plant populations is a major issue in conservation ***,little is known about the partitioning of genetic diversity in alpine species,which occur in naturally fragmented ***,we investigate molecular patterns of three alpine plants(Epilobium fleischeri,Geum reptans and Campanula thyrsoides)across Switzerland and ask whether spatial isolation has led to high levels of population differentiation,increasing over distance,and a decrease of within-population *** further hypothesize that the contrasting potential for long-distance dispersal(LDD)of seed in these species will considerably influence and explain diversity *** For each study species,we sampled 20–23 individuals from each of 20–32 populations across entire *** applied Random Amplified Polymorphic Dimorphism markers to assess genetic diversity within(Nei’s expected heterozygosity,He;percentage of polymorphic bands,Pp)and among(analysis of molecular variance,Ust)populations and correlated population size and altitude with within-population *** patterns of genetic relatedness were investigated using Mantel tests and standardized major axis regression as well as unweighted pair group method with arithmetic mean cluster analyses and Monmonier’s *** avoid known biases,we standardized the numbers of populations,individuals and markers using multiple random *** modelled LDD with a high alpine wind data set using the terminal velocity and height of seed release as key ***,we assessed a number of important life-history traits and factors that potentially influence genetic diversity partitioning(*** system,longevity and population size).Important findings For all three species,we found a significant isolation-by-distance relationship but only a moderately high differentiation among populations(Ust:22.7

关键词： Campanula thyrsoides Epilobium fleischeri Geum reptans isolation by distance molecular diversity

Risk of second primary cancers after testicular cancer in East and West Germany： a focus on contralateral testicular cancers

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Asian Journal of Andrology 2014年第2期16卷 285-289,I0010,I0011页

作者： Carsten Rusner Brigitte Streller Christa Stegmaier Pietro Trocchi Oliver kuss Katherine A McGlynn Britton Traberts Andreas Stang Institute of Clinical Epidemiology Medical Faculty Martin-Luther-University of Halle-Wittenberg Halle (Saale) Common Cancer Registry of Berlin Berlin Saarland Cancer Registry Saarbrucken Institute of Medical Epidemiology Biostatistics and Informatics Medical Faculty Martin-Luther-University of Halle-Wittenberg Halle (Saale) Germany Division of Cancer Epidemiology and Genetics National Cancer Institute National Institutes of Health Bethesda MD Department of Epidemiology Scfloof of Public Heaftfl Boston University Boston MA USA.

Testicular cancer survival rates improved dramatically after cisplatin-based therapy was introduced in the 1970s. However, chemotherapy and radiation therapy are potentially carcinogenic. The purpose of this study was to estimate the risk of developing second primary cancers including the risk associated with primary histologic type （seminoma and non-seminoma） among testicular cancer survivors in Germany. We identified 16 990 and 1401 cases of testicular cancer in population-based cancer registries of East Germany （1961-1989 and 1996-2008） and Saarland （a federal state in West Germany; 1970-2008）, respectively. We estimated the risk of a second primary cancer using standardized incidence ratios （SIRs） with 95% confidence intervals （95% Cls）. To determine trends, we plotted model-based estimated annual SIRs. In East Germany, a total of 301 second primary cancers of any location were observed between 1961 and 1989 （SIR： 1.9; 95% Ch 1.7-2.1）, and 159 cancers （any location） were observed between 1996 and 2008 （SIR： 1.7; 95% Ch 1.4-2.0）. The SIRs for contralateral testicular cancer were increased in the registries with a range from 6.0 in Saarland to 13.9 in East Germany. The SIR for seminoma, in particular, was higher in East Germany compared to the other registries. We observed constant trends in the model-based SIRs for contralateral testicular cancers. The majority of reported SIRs of other cancer sites including histology-specific risks showed low precisions of estimated effects, likely due to small sample sizes. Testicular cancer patients are at increased risk especially for cancers of the contralateral testis and should receive intensive follow-ups.

关键词： cancer registry incidence neoplasms second primary testicular neoplasms

3D printing of silk fibroin-based hybrid scaffold treated with platelet rich plasma for bone tissue engineering

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Bioactive Materials 2019年第1期4卷 256-260页

作者： Liang Wei Shaohua Wu Mitchell kuss Xiping Jiang Runjun Sun Reid Patrick Xiaohong Qin Duan Bin School of Textile Science and Engineering Xi'an Polytechnic UniversityXi'an710048PR China Mary&Dick Holland Regenerative Medicine Program Division of CardiologyDepartment of Internal MedicineUniversity of Nebraska Medical CenterOmahaNE68198USA Key Laboratory of Textile Science&Technology Ministry of EducationCollege of TextilesDonghua UniversityShanghai201620PR China College of Textiles&Clothing Qingdao UniversityQingdao266071PR China Department of Pathology&Microbiology College of MedicineUniversity of Nebraska Medical CenterOmahaNE68198USA Department of Surgery College of MedicineUniversity of Nebraska Medical CenterOmahaNE68198USA Department of Mechanical and Materials Engineering University of Nebraska-LincolnLincolnNE68516USA

3D printing/bioprinting are promising techniques to fabricate scaffolds with well controlled and patient-specific structures and architectures for bone tissue *** this study,we developed a composite bioink consisting of silk fibroin(SF),gelatin(GEL),hyaluronic acid(HA),and tricalcium phosphate(TCP)and 3D bioprinted the silk fibroin-based hybrid *** 3D bioprinted scaffolds with dual crosslinking were further treated with human platelet-rich plasma(PRP)to generate PRP coated ***/Dead and MTT assays demonstrated that PRP treatment could obviously promote the cell growth and proliferation of human adipose derived mesenchymal stem cells(HADMSC).In addition,the treatment of PRP did not significantly affect alkaline phosphatase(ALP)activity and expression,but significantly upregulated the gene expression levels of late osteogenic *** study demonstrated that the 3D printing of silk fibroin-based hybrid scaffolds,in combination with PRP post-treatment,might be a more efficient strategy to promote osteogenic differentiation of adult stem cells and has significant potential to be used for bone tissue engineering.

关键词： 3D bioprinting Hybrid scaffold Coating Growth factor cocktail Tissue engineering

3D printing of multilayered scaffolds for rotator cuff tendon regeneration

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Bioactive Materials 2020年第3期5卷 636-643页

作者： Xiping Jiang Shaohua Wu Mitchell kuss Yunfan Kong Wen Shi Philipp N.Streubel Tieshi Li Bin Duan Mary&Dick Holland Regenerative Medicine Program Division of CardiologyDepartment of Internal MedicineUniversity of Nebraska Medical CenterOmahaNE68198USA Molecular Genetics and Cell Biology Program Department of GeneticsCell Biology and AnatomyUniversity of Nebraska Medical CenterOmahaNE68198USA College of Textiles&Clothing Collaborative Innovation Center of Marine Biomass FibersQingdao UniversityQingdao266071China Department of Orthopedic Surgery and Rehabilitation University of Nebraska Medical CenterOmahaNE68198USA Department of Pediatrics University of Nebraska Medical CenterOmahaNE68198USA Department of Surgery College of MedicineUniversity of Nebraska Medical CenterOmahaNE68198USA Department of Mechanical and Materials Engineering University of Nebraska-LincolnLincolnNE68516USA

Repairing massive rotator cuff tendon defects remains a challenge due to the high retear rate after surgical intervention.3D printing has emerged as a promising technique that enables the fabrication of engineered tissues with heterogeneous structures and mechanical properties,as well as controllable microenvironments for tendon *** this study,we developed a new strategy for rotator cuff tendon repair by combining a 3D printed scaffold of polylactic-co-glycolic acid(PLGA)with cell-laden collagen-fibrin *** designed and fabricated two types of scaffolds:one featuring a separate layer-by-layer structure and another with a tri-layered structure as a *** tensile tests showed that both types of scaffolds had improved mechanical properties compared to single-layered PLGA *** printed scaffold with collagen-fibrin hydrogels effectively supported the growth,proliferation,and tenogenic differentiation of human adipose-derived mesenchymal stem *** implantation of the multilayered scaffolds demonstrated their excellent in vivo *** study demonstrates the feasibility of 3D printing multilayered scaffolds for application in rotator cuff tendon regeneration.

关键词： Collagen-fibrin hydrogel Tenogenic differentiation Tendon repair Tissue engineering

Exosomes derived from differentiated human ADMSC with the Schwann cell phenotype modulate peripheral nerve-related cellular functions

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Bioactive Materials 2022年第8期7卷 61-75页

作者： Bo Liu Yunfan Kong Wen Shi Mitchell kuss Ke Liao Guoku Hu Peng Xiao Jagadesan Sankarasubramanian Chittibabu Guda Xinglong Wang Yuguo Lei Bin Duan Mary&Dick Holland Regenerative Medicine Program and Division of Cardiology Department of Internal MedicineUniversity of Nebraska Medical CenterOmahaNEUSA Department of Pharmacology and Experimental Neuroscience University of Nebraska Medical CenterOmahaNEUSA Department of Genetics Cell Biology&AnatomyCollege of MedicineUniversity of Nebraska Medical CenterOmahaNEUSA Department of Pharmacology&Experimental Neuroscience College of MedicineUniversity of Nebraska Medical CenterOmahaNEUSA Department of Biomedical Engineering Huck Institutes of the Life SciencesSartorius Mammalian Cell Culture FacilityPennsylvania State UniversityUniversity ParkPAUSA Department of Surgery College of MedicineUniversity of Nebraska Medical CenterOmahaNEUSA Department of Mechanical and Materials Engineering University of Nebraska-LincolnLincolnNEUSA

Peripheral nerve regeneration remains a significant clinical challenge due to the unsatisfactory functional recovery and public health ***,especially those derived from mesenchymal stem cells(MSCs),are promising as potential cell-free therapeutics and gene therapy vehicles for promoting neural *** this study,we reported the differentiation of human adipose derived MSCs(hADMSCs)towards the Schwann cell(SC)phenotype(hADMSC-SCs)and then isolated exosomes from hADMSCs with and without differentiation(i.e.,dExo vs uExo).We assessed and compared the effects of uExo and dExo on antioxidative,angiogenic,anti-inflammatory,and axon growth promoting properties by using various peripheral nerve-related *** results demonstrated that hADMSC-SCs secreted more neurotrophic factors and other growth factors,compared to hADMSCs without *** dExo isolated from hADMSC-SCs protected rat SCs from oxidative stress and enhanced HUVEC migration and *** to uExo,dExo also had improved performances in downregulating pro-inflammatory gene expressions and cytokine secretions and promoting axonal growth of sensory neurons differentiated from human induced pluripotent stem ***,microRNA(miRNA)sequencing analysis revealed that exosomes and their parent cells shared some similarities in their miRNA profiles and exosomes displayed a distinct miRNA *** more miRNAs were identified in dExo than in *** upregulated miRNAs,like miRNA-132-3p and miRNA-199b-5p,were highly related to neuroprotection,anti-inflammation,and *** dExo can effectively modulate various peripheral nerve-related cellular functions and is promising for cell-free biological therapeutics to enhance neural regeneration.

关键词： Anti-oxidation Anti-inflammatory Axon growth Neural regeneration microRNA

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采用直觉式编程能便捷焊接机器人

现代制造 2018年第31期 22-23页

作者： Alexander kuss 不详

由于编写程序是一项代价非常昂贵的工作,因此许多中小企业都放弃使用机器人了。所以.弗劳恩霍夫IPA生产技术和自动化研究所的研究人员开发设计了一款专门用于编辑焊接程序的软件.在这一软件的第一阶段测试中.将原来90min的编程任务缩短... 详细信息

由于编写程序是一项代价非常昂贵的工作,因此许多中小企业都放弃使用机器人了。所以.弗劳恩霍夫IPA生产技术和自动化研究所的研究人员开发设计了一款专门用于编辑焊接程序的软件.在这一软件的第一阶段测试中.将原来90min的编程任务缩短到7min内就完成了。

关键词：焊接机器人编程直觉自动化研究所编写程序中小企业焊接程序研究人员

Quantification in LC and GC a practical guide to good chromatographic data edited by Hans-Joachim kuss and Stavros Kromidas.

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