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骨形态发生蛋白对鼠小脑浦肯野细胞发育的重要性

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中国临床康复 2006年第26期10卷 92-94,i0002页

作者：秦丽华 E. bryan Crenshaw Ⅲ 雷季良周长满北京大学医学部解剖学与组织胚胎学系北京市100083 费城儿童医院耳鼻喉科儿童感染中心神经遗传学组

目的:观察骨形态发生蛋白通过Ⅰ型受体传导信号在小脑浦肯野细胞发育中的作用。为揭示临床有关骨形态发生蛋白引起的遗传病发病及治疗提供有力的依据。方法:实验于2004-03/2005-08在美国费城儿童医院Crenshaw实验室和北京大学医学部解... 详细信息

目的:观察骨形态发生蛋白通过Ⅰ型受体传导信号在小脑浦肯野细胞发育中的作用。为揭示临床有关骨形态发生蛋白引起的遗传病发病及治疗提供有力的依据。方法:实验于2004-03/2005-08在美国费城儿童医院Crenshaw实验室和北京大学医学部解剖学神经研究实验室完成。小鼠种类为C57Bl/6j,敲除了骨形态发生蛋白Ⅰ型受体的Bmpr1a和Bmpr1b两个亚型基因,产生了条件性的BMPR-ⅠA和经典性的BMPR-ⅠB受体敲除鼠,破坏了在小脑内的骨形态发生蛋白信号转导。并使用免疫双标calbindin,Tuj1估计小鼠胚胎13.5d和新生幼鼠的浦肯野细胞发育分化情况。结果:①在小鼠刚出生时,正常组鼠的浦肯野细胞都迁移到了小脑,并形成浦肯野细胞层;但在Bmpr1a/Bmpr1b双敲除鼠的浦肯野细胞迁移明显异常,大部分Calbindin阳性的浦肯野细胞呈簇状位于小脑深部,另有部分细胞迁移到了中脑下丘。②免疫双标显示在正常组双标的细胞位于小脑的腹侧缘;但在Bmpr1a/Bmpr1b双敲除组,双标记的浦肯野细胞数量明显减少,并且浦肯野细胞排列紊乱,说明浦肯野细胞的分化受到影响。结论:①骨形态发生蛋白信号对浦肯野细胞的作用可能不是直接的,或许是经过一些中间环节来完成的。②只有两受体基因Bmpr1a和Bm-pr1b同时敲除才能影响骨形态发生蛋白信号对小脑浦肯野细胞发育的作用。

关键词：骨形态发生蛋白质类小鼠,基因敲除浦肯野细胞

Reimagining large river management using the Resist-Accept-Direct(RAD)framework in the Upper Mississippi River

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Ecological Processes 2023年第1期12卷 645-664页

作者： Nicole K.Ward Abigail j.Lynch Erik A.Beever joshua Booker Kristen L.Bouska Holly Embke jeffrey N.Houser john F.Kocik joshua Kocik David j.Lawrence Mary Grace Lemon Doug Limpinsel Madeline R.Magee bryan M.Maitland Owen McKenna Andrew Meier john M.Morton jeffrey D.Muehlbauer Robert Newman Devon C.Oliver Heidi M.Rantala Greg G.Sass Aaron Shultz Laura M.Thompson jennifer L.Wilkening Minnesota Department of Natural Resources Lake CityMN 55041USA U.S.Geological Survey National Climate Adaptation Science CenterRestonVA 22041USA U.S.Geological Survey Northern Rocky Mountain Science CenterBozemanMT 59715USA Department of Ecology Montana State UniversityBozemanMT 59715USA U.S.Fish and Wildlife Service National Wildlife Refuge SystemOak HarborOH 43449USA U.S.Geological Survey Upper Midwest Environmental Sciences CenterLa CrosseWI 54603USA U.S.Geological Survey Midwest Climate Adaptation Science CenterSt.PaulMN 55108USA NOAA NEFSC OronoME 04473USA University of Nebraska-Lincoln LincolnNE 68503USA National Park Service Climate Change Response ProgramFort CollinsCO 80525USA U.S.Fish and Wild-life Service National Wildlife Refuge SystemBloomingtonMN 55437USA Habitat Conservation Division NOAAAlaska RegionAnchorageAK 99513USA Wisconsin Department of Natural Resources MadisonWI 53703USA Aquatic Sciences Center Center for LimnologyUW-MadisonMadisonWI 53706USA U.S.Geological Survey Northern Prairie Wildlife Research Center and Midwest Climate Adaptation Science CenterJamestownND 58401USA U.S.Army Corps of Engineers La CrescentMN 55947USA Alaska Wildlife Alliance AnchorageAK 99520USA U.S.Geological Survey Alaska Cooperative Fish and Wildlife Research UnitUniversity of Alaska FairbanksFairbanksAK 99775USA University of North Dakota Grand ForksND 58202USA Minnesota Department of Natural Resources DuluthMN 55804USA Wisconsin Department of Natural Resources Boulder JunctionWI 54512USA Great Lakes Indian Fish and Wildlife Commission(GLIFWC) OdanahWI 54861USA U.S.Geological Survey National Climate Adaptation Science Centerand School of Natural ResourcesUniversity of TennesseeKnoxvilleTN 37996USA U.S.Fish and Wildlife Service National Wildlife Refuge SystemFort CollinsCO 80526USA

Background Large-river decision-makers are charged with maintaining diverse ecosystem services through unprec-edented social-ecological transformations as climate change and other global stressors *** interconnected,dendritic habitats of rivers,which often demarcate jurisdictional boundaries,generate complex management ***,we explore how the Resist–Accept–Direct(RAD)framework may enhance large-river management by promoting coordinated and deliberate responses to social-ecological trajectories of *** RAD frame-work identifies the full decision space of potential management approaches,wherein managers may resist change to maintain historical conditions,accept change toward different conditions,or direct change to a specified future with novel *** the Upper Mississippi River System,managers are facing social-ecological transformations from more frequent and extreme high-water *** illustrate how RAD-informed basin-,reach-,and site-scale decisions could:(1)provide cross-spatial scale framing;(2)open the entire decision space of potential management approaches;and(3)enhance coordinated inter-jurisdictional management in response to the trajectory of the Upper Mississippi River *** The RAD framework helps identify plausible long-term trajectories in different reaches(or subbasins)of the river and how the associated social-ecological transformations could be managed by altering site-scale *** reach-scale objectives may reprioritize how,where,and when site conditions could be altered to contribute to the basin goal,given the basin’s plausible trajectories of change(e.g.,by coordinating action across sites to alter habitat connectivity,diversity,and redundancy in the river mosaic).Conclusions When faced with long-term systemic transformations(e.g.,>50 years),the RAD framework helps explicitly consider whether or when the basin vision or goals may no longer be achievable,and direct options may open y

关键词： Ecosystem Management Transformation Social-ecological system Anthropocene Climate change Basin planning Cross-scale interactions

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硅的“第二春”

科技纵览 2014年第8期 34-41页

作者： Cheuk Chi Lo john j.L. Morton bryan Christie Design

宏大的工程挑战往往需要巨大的耐心。这对于量子计算来说是千真万确的。经过漫长的20年，如今我们已经从原理上得出“量子计算会无比强大”的结论。量子计算机在短短几分钟内就能解决普通计算机需要花费天文级的时间才能够计算出的问题... 详细信息

宏大的工程挑战往往需要巨大的耐心。这对于量子计算来说是千真万确的。经过漫长的20年，如今我们已经从原理上得出“量子计算会无比强大”的结论。量子计算机在短短几分钟内就能解决普通计算机需要花费天文级的时间才能够计算出的问题。但是，制造这种机器的工作几乎还没有越过起跑线。事实上，我们仍在试图寻求最佳的构建材料。

关键词：量子计算机硅宏

肯尼迪宇宙中心所用材料的高压液氧、气氧冲击敏感性评定

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导弹与航天运载技术(中英文) 1979年第6期 91-133页

作者： Coleman j bryan john F 徐雪英汪夭汉

本文对十二种材料在液氧、增压液氧及高压气氧中的反应活性进行了评述。这些材料包括;铝合金(6061-T6)、聚四氟乙烯、四种填充聚四氟乙烯、聚酰亚胺、聚三氟氯乙烯、两种氟弹性体、全氟醚基润滑脂及尼龙聚合物。

关键词：气氧靶场白沙试验场机械冲击试验反应次数牛顿试样直径试验次数泰氟隆液化气体液氧肯尼迪宇宙敏感性

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无先前已知心脏病儿童的电轴左偏

中国循证儿科杂志 2019年第4期14卷 319-320页

作者： Andrew E. Schneider bryan C. Cannon jonathan N. johnson Michael j. Ackerman Philip L. Wackel 赵趣鸣(译) 刘芳(校) Division of Pediatric Cardiology Department of Pediatric and Adolescent MedicineMayo ClinicRochesterMinnesota Division of Heart Rhythm Services Department of Cardiovascular DiseasesMayo ClinicRochesterMinnesota Department of Molecular Pharmacology and Experimental Therapeutics and Windland Smith Rice Sudden Death Genomics Laboratory Mayo ClinicRochesterMinnesota 不详

研究背景在儿童中,电轴左偏与一些特殊类型的心脏病理有关,许多患儿在婴儿期得到确诊;但目前尚不清楚无症状的电轴左偏儿童的心脏病发生率,也不清楚这类儿童需要做哪些其他检查。研究新知本研究确认,无先前已知心脏病的电轴左偏儿童可... 详细信息

研究背景在儿童中,电轴左偏与一些特殊类型的心脏病理有关,许多患儿在婴儿期得到确诊;但目前尚不清楚无症状的电轴左偏儿童的心脏病发生率,也不清楚这类儿童需要做哪些其他检查。研究新知本研究确认,无先前已知心脏病的电轴左偏儿童可能存在合并心脏病的危险因素,这些危险因素可以帮助临床医师在偶然发现电轴左偏时,决定哪些患儿应做进一步的检查。

关键词：电轴左偏心脏病理儿童危险因素临床医师婴儿期发生率无症状

Development and Application of a Validated UHPLC Method for the Determination of Atropine and Its Major Impurities in Antidote Treatment Nerve Agent Auto-Injectors (ATNAA) Stored in the Strategic National Stockpiles

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Pharmacology & Pharmacy 2017年第1期8卷 15-31页

作者： Cheng H.Yen Adil Mohammad Miah Schneider Salwa K.Poole bryan Lowry Brenda W.Mc Curdy Patrick j.Faustino Saeed R.Khan Office of Regulatory Affairs Detroit LaboratoryFood and Drug AdministrationDetroitMIUSA Division of Product Quality Research Office of Testing and ResearchOffice of Pharmaceutical QualityCenter for Drug Evaluation and ResearchFood and Drug AdministrationSilver SpringMDUSA

The development and implementation of advanced analytical technologies is essential for extending the expiry for complex drug products stored in the Strategic National Stockpiles. Consequently, a novel Ultra High-Performance Liquid Chromatographic (UHPLC) method has been developed for the analysis of atropine and its respective impurities to support the analytical research platform for auto-injectors. This study is part of a larger research effort to improve the efficiency and broaden the applicability of advanced analytical methods for medical counter-measure medications. The current HPLC compendial methodology for atropine sulfate injection requires an analysis time of 40 minutes for atropine. In comparison, the novel gradient UHPLC method required only 8 minutes to evaluate both atropine and its major pharmaceutical impurities. Improved separation was achieved on a Waters Acquity UHPLC BEH C18 1.7 μm, 2.1 × 100 mm column employing gradient elution of mobile phase solvent A (0.1% H3PO4) and solvent B (0.1% H3PO4, 90% ACN, and 10% H2O). The method was validated according to USP Category I requirements for assay. The daily standard calibration curves were linear over a concentration range from 50 μg/mL to 250 μg/mL with a correlation coefficient of >0.999. The detection limit (LOD) and quantitation limit (LOQ) were 3.9 μg/ml and 13.1 μg/ml, respectively. Resolution results indicate that atropine and the following impurities, degradants and a preservative can also be separated and analyzed using this proposed method: noratropine, 4,4’-di-hy-droxydiphenyl ether, 2,4’-dihydroxydiphenyl ether, 4-bromophenol, 4-hydro-xyatropine, tropic acid, apoatropine HCl, atropic acid, hydroquinone, nitroethane, phenol and catechol. The UHPLC method demonstrated enhanced selectivity and significantly reduced the analysis time when compared with the traditional USP compendial HPLC method. The method was successfully applied to the evaluation of atropine in ATNAA auto-injectors lots fro

关键词： Atropine Sulfate Atropine ATNAA SLEP UHPLC Impurities

INCREASING COVERAGE AND CONFIDENCE IN PROTEIN IDENTIFICATIONS FROM COMPLEX SAMPLES USING MICROFLUIDIC NANO LC/MS/MS

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INCREASING COVERAGE AND CONFIDENCE IN PROTEIN IDENTIFICATION...

第十一届北京分析测试学术报告会暨展览会(BCEIA 2005)

作者： shuolin SONG Christine A.MILLER bryan D.MILLER David M.HORN Frank E.KUHLMANN Michael j.MANFREDI Agilent Technologies Inc.

It is generally acknowledged that the Mud PIT approach to proteomic profiling of complex samples provides confident indication of only a subset of the actual proteins present in the *** number of confident protein ide...

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环境监测—一个实例研究

产业与环境 1992年第1期14卷 3-7页

作者：方兆珩 C.W.bryan j.D.Cargill Clayton Bostock Hill and Rigby

一家公司在现有工厂中引进一种新制造工艺时,也需要进行新的化学品贮存和分配、水处理、废水处理和废气处理。该公司有强有力的法人环境政策,虽然并没有义务这样做,但该公司仍然实施了环境影响评价和环境监测计划。该监测计划始于新工... 详细信息

一家公司在现有工厂中引进一种新制造工艺时,也需要进行新的化学品贮存和分配、水处理、废水处理和废气处理。该公司有强有力的法人环境政策,虽然并没有义务这样做,但该公司仍然实施了环境影响评价和环境监测计划。该监测计划始于新工艺开工前九个多月,提供了有关大气、土壤、地下水和地表水的质量的基线数据。监测继续进行,以便评价该工艺的影响,并为废水和大气排放物治理装置的设计提供有用信息。自从原工艺采用以来,制造技术上已经有了二个重大变化。这些变化也需要进一步监测,主要是废水水质和大气排放方面的监测,以便改进其处理工艺。

关键词：环境监测

Effect of Biochar Type and Size on<i>in Vitro </i>Rumen Fermentation of Orchard Grass Hay

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Agricultural Sciences 2017年第4期8卷 316-325页

作者： Zach D. McFarlane Phillip R. Myer Emily R. Cope Neil D. Evans T. Carson Bone bryan E. Biss j. Travis Mulliniks Department of Animal Science University of Tennessee Knoxville TN USA Proton Power Lenoir City TN USA

Biochar, a byproduct from the biofuels industry, may be a potential feed additive in ruminant diets due to possible improvements in microbial fermentation. Therefore, the objective of this study was to determine the nutritive value, in vitro digestibility, volatile fatty acid (VFA) production, and gas production of biochar inclusion to an orchard grass (Dactylis glomerata) basal diet. The study was designed as a 3 × 2 factorial arrangement with 3 different biochar sources and 2 biochar processed sizes as the main effects factors. Experimental treatments were biochar from 3 different tree types: 1) Chestnut Oak (Quercus prinus L.;CO), 2) Yellow Poplar (Liriodendron tulipifera;YP), or 3) White Pine (Pinus strobus L.;WP), and processed at 2 different biochar particle sizes: a) 178 μm (Coarse). Biochar was added to the basal diet of orchard grass hay (872.35 g/kg of DM, 98.31 g/kg of CP, and 704.02 g/kg of aNDF, DM basis) at a rate of 81 g/kg DM. Biochar residual ash content was greater (P P P = 0.01) with lower aNDF content in both WP sizes compared with their respective biochar type and size. Gas production was not influenced (P = 0.23) by biochar tree type;however, gas production was increased (P = 0.05) by Fine particle size compared with Coarse biochar. The in vitro true digestibility (IVTD) of orchard grass hay was increased (P = 0.01) by the inclusion of Fine biochar particle size compared with Coarse particle size. Additionally, in vitro CP true digestibility (DCP) exhibited a type × size interaction (P = 0.01). Crude protein digestibility was lower for Fine particle-sized CO and WP biochar sources compared with Coarse particle-sized CO and WP (P ≤ 0.004). However, DCP was not different between Coarse and Fine particle-sized YP biochar (P = 0.70). Volatile fatty acids (acetate, propionate, and butyrate) were not altered by biochar type (P ≥ 0.66) or particle size (P ≥ 0.19). These results indicate that both tree type and particle size of biochar may need to be c

关键词： Biochar Forage Digestibility Gas Production Rumen Fermentation