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Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells

World Journal of Stem Cells 2020年第12期12卷 1640-1651页

作者： Cintia DS Horinouchi María Julia Barisón Anny W Robert Crisciele Kuligovski Alessandra M Aguiar brunodallagiovanna Stem Cells Basic Biology Laboratory Instituto Carlos ChagasCuritiba 81350010ParanáBrazil

BACKGROUND Human adipose-derived stromal/stem cells(hASCs)are one of the most useful types of mesenchymal stromal/stem cells,which are adult multipotent cells with great therapeutic potential for the treatment of several ***,for successful clinical application,it is critical that high-quality cells can be *** factors seem to be able to influence cell quality and performance,especially factors related to donors’intrinsic characteristics,such as ***,there is no consensus regarding this characteristic,and there is conflicting information in the *** To investigate the growth kinetics and differentiation potential of adipose-derived stem cells isolated from the lipoaspirates of elderly and young *** hASCs were harvested from liposuctioned adipose tissue obtained from female donors(aged 20-70 years).Cells were distributed into two groups according to age range:old hASCs(oASCs,≥55 years,n=9)and young hASCs(yASCs,≤35 years,n=9).For each group,immunophenotypic characterization was performed by flow *** doubling time was assessed over seven *** adipogenic potential evaluation,lipid deposits were assessed after 7 d,14 d and 21 d of adipogenic *** potential was verified by analyzing cell mineralization after 14 d,21 d and 28 d of osteogenic *** expression of PPARγ2,CEBPA and Runx2 were detected by quantitative reverse transcription polymerase chain *** hASCs were successfully obtained,cultured,and grouped according to their age:yASCs(26.33±4.66 years old)and oASCs(64.78±4.58 years old).After maintenance of the cells in culture,there were no differences in morphology between cells from the young and old ***,both groups showed classical immunophenotypic characteristics of mesenchymal stem/stromal *** average doubling time indicated that yASCs(4.09±0.94 d)did not significantly differ from oASCs(4.19±1.29 d).Concerning differentiation pot

关键词： Adipose-derived stem cells Stem cells Adipogenesis Osteogenesis Cell differentiation Donor age

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