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Phytochemical bioprospecting, antioxidant, antimicrobial and cytotoxicity activities of saline extract from Tithonia diversifolia(Hemsl) *** leaves

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Asian Pacific Journal of Tropical Biomedicine 2018年第5期8卷 245-253页

作者： bruno rafael barboza Bárbara rafaela da Silva Barros Bárbara de Azevedo Ramos Maiara Celine de Moura Thiago Henrique Napoleao Maria Tereza dos Santos Correia Luana Cassandra Breitenbach Barroso Coelho Iranildo José da Cruz Filho Ana Maria Souto Maior Túlio Diego da Silva Leylianne de Cássia Rodrigues Nerys Edson Renan Barros de Santana Cláudia Sampaio de Andrade Lima Virgínia Maria Barros de Lorena Cristiane Moutinho Lagos de Melo Department of Antibiotics Biosciences Centre Federal University of Pernambuco Department of Biochemistry Biosciences Centre Federal University of Pernambuco Center for Strategic Techmologies of the Northeast Department of Biophysics Biosciences Centre Federal University of Pernambuco Aggeu Magalhaes Institute IAM/FIOCRUZ

Objective:To evaluate antimicrobial and antioxidant properties of saline extract from Tithonia diversifolia leaves by phytochemical bioprospecting,and investigate its safety against animal ***:The saline extract was prepared,with NaCl(0.15 M),by constant stirring of the dried and pulverized leaves,followed by volume reduction by *** extract was phytochemical characterized using ultra-performance liquid chromatography,and total phenol and flavonoid analysis also was *** antioxidant capacity was determined through DPPH*radical,the antimicrobial property was evaluated against standard bacteria and fungi,and the viability assays were performed against mice ***:Fifteen compounds were identified belonging to two main classes terpenoids and *** extract showed 22.185 mg GAE/g of total phenolic compounds and 3.220 mg QE/g of ***,extract showed higher antioxidant ability similar to butylated hydroxytoluene a standard molecule[(3.042±0.019)mg AAE/g and(4.12±0.10)mg AAE/g to saline extract and butylated hydroxytoluene,respectively].The antimicrobial assays demonstrated that the extract had a significant antifungal potential against Candida species and could be used with safety against mice splenocytes,in concentrations lower than 50μg/mL,promoting higher proliferation in these ***:Saline extract from Tithonia diversifolia leaves presents potential antioxidant,antifungal properties and induces immunostimulation in mice splenocytes.

关键词： Tithonia diversifolia Extract Antioxidant Antifungal properties Immunostimulation

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Device performance and light characteristics stability of quantum-dot-based white-light-emitting diodes

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Nano Research 2018年第3期11卷 1575-1588页

作者： bruno Clasen Hames Ivan Mora-Sero rafael S. Sanchez Institute of Advanced Materials (INAM) Universitat Jaume 1 12071 Castello Spain

Advances in image quality in recent decades have made it necessary to develop new technologies for producing displays to meet remarkably stricter standards. The display market is governed mainly by liquid crystal display and light-emitting diode （LED） technology; however, it suffers from limitations that can be overcome by developing the next generation of electroluminescent displays. The introduction of colloidal quantum dots （QDs） as down-converters has enabled the production of displays with extremely high color purity and gamut. Therefore, colloidal nanocrystals are excellent candidates for the preparation of electroluminescent devices, which represent a straightforward approach to the development of unprecedented high-quality displays. We synthesized light-emitting QDs covering the entire visible spectrum with high fluorescence quantum yields and color purity, and produced high-brightness single-color LEDs with external quantum efficiencies of 0.39%, 1.04%, 2.10%, and 1.30% for red-, orange-, green-, and blue-emitting dots, respectively. Additional136 white LEDs were prepared by mixing QDs; they showed color temperatures of 5,300 K and color rendering indices exceeding 80%. Very importantly, we exhaustively characterized the LED performance, including the response time, stability, and evolution of the light characteristics, thus providing crucial information toward the development of high-quality electroluminescent displays.

关键词： colloidal quantum dots quantum dotlight-emitting diodes white light-emitting diode(LED) displays electroluminescence LED stability

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Air Basins of Rio de Janeiro State, Brazil

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Journal of Water Resource and Protection 2015年第10期7卷 781-791页

作者： rafael S. barboza Ricardo Valcarcel Ednaldo O. Santos Carlos R. Pereira Laboratory of Catchment Basins Management Department of Environmental Sciences Forestry Institute Federal Rural University of Rio de Janeiro Seropédica Brazil Department of Agricultural Engineering and Environment School of Engineering Fluminense Federal University Niteroi Brazil

The air basin is a three-dimensional space which conducts and distributes winds over the catchment basins. In the state of Rio de Janeiro (43,305 km2), 48% of the territory comprises the Atlantic slopes (the windward side) and 52% (the leeward side) suffers the influence of the Atlantic Ocean. On the windward side, 20% of the area includes 16 air basins, which influence the movement of humid air currents, affecting all of the windward regions and environmentally conditioning the leeward areas. The morphometric parameters of these air basins have been characterized: area of influence upwind (4 parameters), slope (5 parameters), and distribution zone (5 parameters), and they are grouped together in 5 units by similar functional identity, utilizing multivariable analysis, cluster method, with a Euclidian distance of 48%, significance level of 95%, and correlation coefficient of 0.7132. The management of environmental services within the hydro-graphic basins where 16 million people live is conditioned by these air basins, which could have their environmental planning perfected by this knowledge.

关键词： Landscape Fog Interception Water Resources Watershed

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Biomass-glycerol briquettes are not necessarily mechanically stable and energetically effective

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Waste Disposal and Sustainable Energy 2020年第4期2卷 291-303页

作者： bruno rafael de Almeida Moreira Ronaldo da Silva Viana Samuel Dias Moreira Monique dos Santos Lima Anderson Chagas Magalhães Celso Tadao Miasaki JoséClaudio Caraschi Department of Phytosanitary Rural Engineering and SoilsSchool of EngineeringSão Paulo State University(Unesp)Ilha SolteiraSão PauloBrazil Department of Plant Production College of Agricultural and Technological SciencesSão Paulo State University(Unesp)DracenaBrazil São Paulo State University(Unesp) ItapevaSão PauloBrazil

The re-use of glycerol from biodiesel industry as an alternative lubricant for making high-performance briquettes is ***,the technical performance of this agro-industrial residue is not *** study outlines,accordingly,the real risk of introducing glycerol into the co-briquetting of highly caloric by-products of *** production of hybrid briquettes consisted of pressing mixtures of residues of sugarcane and sorghum with the liquid additive at 10,20,and 30 wt.%in bench-scale hydraulic piston presser *** of the blend,briquettes containing the supplement at the highest level as part of their composition ended up being much more hygroscopic(20.10%)and less energetic(3.15 GJ m^(−3)).The explanation for the negative impact of glycerol on the thermomechanical behavior of briquettes would be overconcen-tration of additive capable of effectively defying compacting *** degree of compaction during co-briquetting has likely gone down quickly with a maximizing level of *** long as the additive is not able to lubricate the feedstock suitably,improbability of biomass particles to successfully bond together to form themselves into mechanically stable and energetically effective briquettes is *** lower density(273.80 kg m^(−3))and higher relaxation(22.75%),briquettes with glycerol at 30 wt.%generally were aesthetically ***,these products resisted no longer to handling,transportation,and *** lost their shape easily during emptying and shifting them,thus,releasing larger quanti-ties of biomass to the *** evidence of high-viscosity glycerol capable of limiting safe and effective production of high-performance briquettes for heating and power exists.

关键词： Additive Agro-residues Densification Biosolids Waste-to-energy

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Investigations on the Thermodynamic Stability and Availability of Nutrients for Plants by Humic Substances Extracted from Peat Samples

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Journal of Environmental Science and Engineering(A) 2015年第7期4卷 367-377页

作者： Camila De Almeida Melo Lilian Karla De Oliveira bruno barboza Cunha Leonardo Femandes Fraceto Andre Henrique Rosa Department of Environmental Engineering Sao Paulo State University (UNESP) Sorocaba 18087-180 Sao Paulo State Brazil

This work evaluated the complexation capacity, exchange constants and availability of micronutrients for plants and humic substances extracted from peat samples. Samples of humic substances extracted from two tropical peats （HS-P1 and HS-P2） were enriched with the micronutrients Cu（II）, Co（II）, Fe（II）, Mn（II）, Ni（II） and Zn（II） and the parameters for formation of the complexes （HS-N） were evaluated at different pH. The Scatchard model was used to calculate the maximum complexation capacity and the nutrient availability was studied using exchange capacity experiments based on ultrafiltration procedure. The optimum pH for complexation was 4.5 and the order of affinity was： Fe（II）〉 Cu（II）〉 Co（II）〉 Mn（II） = Ni（II）〉 Zn（II）. The maximum complexation capacity reached 56.8 mg·g-1 Fe of HS-P1 （the highest） and 1.7 mg.g1 Zn of HS-P2 （the slightest）. The exchange experiments showed that HS-P-Fe complexes were formed preferentially. The least stable complex was formed with Zn, which was therefore, more easily available. The results contribute to understand the behavior and availability of some nutrients in soils.

关键词： Peat humic substances complexation nutrient availability exchange capacity soil

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Valorization of semi-solid by-product from distillation of cellulosic ethanol into blends for heating and power

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Waste Disposal and Sustainable Energy 2021年第1期3卷 49-61页

作者： bruno rafael de Almeida Moreira Victor Hugo Cruz Matheus Luís Oliveira Cunha Nathaly Proença Lopes Anderson Chagas Magalhães Celso Tadao Miasaki JoséClaudio Caraschi Ronaldo da Silva Viana Department of Phytosanitary Rural Engineering and SoilsSchool of EngineeringSão Paulo State University(Unesp)Ilha SolteiraSão PauloBrazil Department of Plant Production College of Agricultural and Technological SciencesSão Paulo State University(Unesp)DracenaSão PauloBrazil São Paulo State University(Unesp) ItapevaSão PauloBrazil

Burning down on semi-solid by-product from distillation of cellulosic ethanol to power upstream steps of its manufacturing is ***,this feedstock is homogenous in size and shape,hygroscopic and poorly *** disadvantages make its re-use in the industrial plant very *** of this agro-residue into high-performance blends for heating and power is,accordingly,the scientific point of this *** pilot-scale manufacturing of composite pellets consisted of systematically pressing the residue with sugarcane bagasse at the mass ratios of 1:4,2:4,and 3:4 on an automatic pelletizer machine at 200 MPa and 125℃.The process of compaction lasted for 90 *** and energy density,both increased significantly,from 95.85%to 99.55%and from 27.95 GJ m^(−3) to 32.20 GJ m^(−3) with blending at 3:***,the semi-solid by-product considerably improved the feeding,thus,enabling the layers of particles to go smoothly through the channel-forming die of the ***,blends became stiffer and denser than pellets purely consisting of sugarcane *** evidence of the process of pelleting capable of highly valorizing the semi-solid by-product from distillation of cellulosic bioethanol into mechanically stable and energetically effective hybrid fuel grade biosolids *** major findings of this paper should be of great relevance to ensure safe and effective transportation and storage of biomass in indoor facili-ties where the risk for the generation of dust and fines and the subsequent off-gassing and self-firing is ***,pelletization may optimize the co-generation of heat and steam at the large-scale bioethanol station.

关键词： Fuel grade biosolids Non-woody biomass Pelletization Second-generation biofuel Sustainable waste-to-energy technique

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Sublethal Doses of Insecticides Affect the Fecundity and Fertility of the <i>Chrysodeixis includens</i>?

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American Journal of Plant Sciences 2018年第3期9卷 483-494页

作者： Raimundo Henrique Ferreira Rodrigues Luciana barboza da Silva Thiago Ferreira Rodrigues Gleidyane Novais Lopes Eliane Carneiro bruno Ettore Pavan Post-Graduation Program in Plant Science Agronomy Universidade Federal do Piauí Bom Jesus Brazil Faculty of Engineering UNESP Ilha Solteira Universidade Estadual Paulista Ilha Solteira Brazil

The objective was to determine lethal concentrations and to evaluate the effects of sublethal doses of insecticides on Chrysodeixis includens under laboratory conditions. The standard population of C. includens was kept on artificial diet under controlled conditions (25°C ± 5°C, 60% ± 10 RU, 12:12 h). The active principles used were B. thuringiensis, Indoxacarb and Teflubenzuron. The CL25 and LC50 obtained from dose response curve (estimated from eight concentrations of each active principle) were diluted in water and applied to the back of third instar caterpillars and compared to the control (water). The insecticides used showed lethal and sublethal effects in C. includens. Bacillus thuringiensis was 6.21 and 2.79 times more toxic to soybean looper when compared to the products Indoxacarb and Teflubenzuron, respectively. Insecticides applied to the caterpillars reduced survival and affected the biomass gain with increased larvae and pupae longevity, with a significant reduction in fertility (40%) and fecundity (94.97%) in adults. In the present study it was observed that both survival and larval biomass gain have a direct effect on fecundity and fertility, respectively. Therefore the active ingredients Indoxacarb, Teflubenzuron and B. thuringiensis can be used in rotation as a tool in the C. includens.

关键词： Bacillus thuringiensis Chemical Control Indoxacarb Soybean Looper Teflubenzuron

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Resistance of Two Maize Landraces in Breeding Stage to the Attack of <i>Sitophilus zeamais</i>

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American Journal of Plant Sciences 2014年第20期5卷 2929-2934页

作者： Rejane Teixeira do Nascimento bruno Ettore Pavan Luciana barboza Silva Gabriel dos Santos Carvalho Alexandre Faria da Silva Kellen Maggioni Graduate Program in Agronomy-Crop Science Federal University of Piauí Bom Jesus Brazil College of Engineering of Ilha Solteira—DFTASE—UNESP S&atildeo Paulo Brasil

This study aimed to assess the resistance of grain from two varieties of maize landraces in initial breeding in Bom Jesus-PI, with respect to S. zeamais attack. Seeds of two maize landraces were used: purple straw maize from two different origins (São Paulo (SP) and Espírito Santo (ES)) and Peruvian purple maize. The experimental design was completely randomized with four replications. The data for each response variable were subjected to analysis of variance, applying the F-test (p ≤ 0.05), and when significant differences were found, there was a comparison of means by Tukey test (p ≤ 0.05). The statistical analyses were performed using the software SAS®. In a free-choice test and bioassay for evaluating the nutritional index (no choice test), maize varieties differed significantly by Tukey test and the cultivar SP was considered resistant, while the cultivars purple and ES were considered susceptible.

关键词： Selection Genetic Variability Susceptibility

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Trace metal dynamics in an industrialized Brazilian river:A combined application of Zn isotopes,geochemical partitioning,and multivariate statistics

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Journal of Environmental Sciences 2021年第3期33卷 313-325页

作者： Myller S.Tonhá Daniel F.Araújo rafael Araújo bruno C.A.Cunha Wilson Machado Joelma F.Portela João PR Souza Hikari K.Carvalho Elton L.Dantas Henrique L.Roig Patrick Seyler Jeremie Garnier Universidade de Brasília Instituto de GeociênciasCampus Darcy RibeiroL2Asa Norte70910900 BrasíliaDistrito FederalBrazil Laboratoire de Biogéochimie des Contaminants Métalliques IfremerCentre AtlantiqueF44311 Nantes Cedex 3France Institute of Geosciences University of São PauloRua do Lago562São Paulo 05508-080Brazil Universidade Federal Fluminense Programa de GeoquímicaCampus do ValonguinhoNiteróiRio de JaneiroBrazil Analytical and Environmental Chemistry Laboratory Instituto de QuímicaUniversity of BrasiliaBrasiliaFederal District 70919-970Brazil Laboratoire Mixte International“Observatoire des Changements Environnementaux”(LMI OCE) Institut de Recherche pour le Développement/University of BrasiliaCampus Darcy RibeiroBrasiliaBrazil Hydrosciences Montpellier Universitéde MontpellierInstitut de Recherche pour le développementCentre National de la Recherche ScientifiqueMontpellierFrance

The Paraiba do Sul(PSR)and Guandu Rivers(GR)water diversion system(120 km long)is located in the main industrial pole of Brazil and supplies drinking water for 9.4 million people in the metropolitan region of Rio de *** study aims to discern the trace metals dynamics in this complex aquatic *** used a combined approach of geochemical tools such as geochemical partitioning,Zn isotopes signatures,and multivariate *** and Pb concentrations in Suspended Particulate Matter(SPM)and sediments were considerably higher in some *** sediment partition of As,Cr,and Cu revealed the residual fraction(F4)as the main fraction for these elements,indicating low *** and Pb were mostly associated with the exchangeable/carbonate(F1)and the reducible(F2)fractions,respectively,implying a higher susceptibility of these elements to being released from *** isotopic compositions of sediments and SPM fell in a binary mixing source process between lithogenic(δ^(66/64)Zn_(JMC)≈+0.30‰)and anthropogenic(δ^(66/64)Zn_(JMC)≈+0.15‰)end *** lighterδ^(66/64)Zn_(JMC)values accompanied by high Zn concentrations in exchangeable/carbonate fraction(ZnF1)enable the tracking of Zn anthropogenic sources in the studied ***,the results indicated that Hg,Pb,and Zn had a dominant anthropogenic origin linked to the industrial activities,while As,Cr,and Cu were mainly associated with lithogenic *** work demonstrates how integrating geochemical tools is valuable for assessing geochemical processes and mixing source effects in anthropized river watersheds.

关键词： Metal pollution Isotope tracer Sequential extraction Anthropogenic activity

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Evaluation of Insecticides Targeting Control of Helicoverpa armigera(Hubner)(Lepidoptera:Noctuidae)

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American Journal of Plant Sciences 2014年第18期5卷 2823-2828页

作者： Eliane Carneiro Luciana barboza Silva Kellen Maggioni Vilmar Buenos dos Santos Thiago Ferreira Rodrigues Soislan Souza Reis bruno Ettore Pavan Programa de Pos Graduacao em Agronomia Fitotecnia Universidade Federal do PiauíCampus Prof.CinobelinaElvasBom JesusBrazil Graduacao em Agronomia Universidade Federal do Piauí Campus Prof.CinobelinaElvasBom JesusBrazil Faculdade de Engenharia UNESP Ilha Solteira Universidade Estadual PaulistaIlha SolteiraBrazil

Insecticides from different chemical groups were tested by laboratory bioassay to verify the percentage mortality of Helicoverpa armigera (Hubner 1808) (Lepidoptera: Noctuidae). The experiment was conducted in the Crop Science laboratory—Prof. CinobelinaElvas Campus—UFPI, Bom Jesus, PI, from January to June, 2013. The populations utilized came from the University’s own insect breeding laboratories. Third instar larvae of H. armigera were used to conduct the bioassay. The experimental design was fully randomized, with 13 treatments and four replications. Five larvae were used per replication, with 12 insecticides from 9 different chemical groups and a control. Each treatment consisted of three doses. The methods of application used were topical contact and ingestion in artificial diet. According to the results the percentage mortality of H. armigera larvae varied among the treatments. The results demonstrated that chlorpyrifos and spinosad were effective against third instar H. armigera larvae both on contact and by ingestion. Flubendiamide, acephate, methomyl, Bacillus thuringiensis, dimethoate, chlorantraniliprole and fipronil had good responses to control of H. armigera.

关键词： Bollworm Bioassay Chemical Control Insecticides Soybeans

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