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Study of Behavioral Changes and Photosynthetic Activity of <i>Euglenas gracilis</i>in the Presence of Effluents from the Laboratory of Clinical Analysis

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Journal of Environmental Protection 2020年第12期11卷 1015-1029页

作者： Luciano Henrique Pinto Suellen Zucco Bez Julia Carolina Soares bruna bonfim Qushmua Alzahrani Assistant Professor at the Department of Pharmacy/Nursing/Medicine in UNIVILLE Joinville SC Brazil Student at the Pharmacy Course in UNIVILLE Joinville SC Brazil Voluntary Researcher in UNIVILLE Joinville SC Brazil

Healthcare waste has now been increasingly studied in terms of the risks or dangers that can cause the environment and human health. Waste generated in clinical analysis laboratories (CALs) deserves attention, because, due to the advent of the concept of emergent pollution, it is doubtful if the materials or reagents are disposed in the sewage by CALs, which are currently considered non-contaminated or with low risk potential, under current legislation, may actually impact the environment with actions not yet understood. This study was experimental and conducted at the Environmental Laboratory of the University of the Region of Joinville. It was used Euglena gracilis (primary trophic level) algae exposed to effluents from five sectors of a CAL: Biochemistry, Hematology, Viral Load, Tuberculosis and Immunochemistry. Samples were collected from the siphons attached to the wash sinks of the CAL materials. To verify changes in algae that denote environmental danger, behavioral changes were analyzed via NGTOX, and chlorophyll concentration was calculated by chlorophyll extraction according to Mendel’s method. Viral Load (VL) and Hematology (HT) sectors were the ones that most affected algae (Tukey test). In both sectors, there was inhibition of algae mobility and gravitaxy: in VL, due to the presence of chaotropic agents that denature organic structures;and in HT, due to the change in membrane permeability attributed to methylene blue. Also in HT, there was a search for algae adaptation by increasing the rise to the surface in order to overcome the lower luminosity due to the coloration of the environment, which also affects photosynthesis. Regarding the concentration of a-chlorophyll, the VL and HT were the most affected as well, being the first one the one that had more concentration reduction because of the presence of chaotropic agents. Considering new parameters evaluated, the discarded compounds need to be better evaluated for risk, as they affect algal photosynthe

关键词： Ecotoxicity Emerging Pollutants Chaotropic Agent Methylene Blue Effluent from Clinical Analysis Laboratory Residues

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Study of Behavioral Changes and Photosynthetic Activity of Euglenas gracilis in the Presence of Effluents from the Laboratory of Clinical Analysis

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Journal of Environmental Protection（环境保护） 2020年第12期

作者： Luciano Henrique Pinto Suellen Zucco Bez Julia Carolina Soares bruna bonfim Qushmua Alzahrani

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Allelopathy of the Leaf Extract of Eucalyptus Genetic Material on the Physiological Performance of Millet Seeds

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American Journal of Plant Sciences 2018年第1期9卷 34-45页

作者： Marcos Vinicius de Sousa Séfora Gil Gomes de Farias Déborah Praciano de Castro Romário Bezerra e Silva Dandara Yasmim bonfim de Oliveira Silva bruna Anair Souto Dias Alexandre Faria da Silva Graziele Nunes Lopes dos Santos Daniele Cristina Pereira de Matos Caio Varonill de Almada Oliveira Universidade Federal do Piauí (UFPI) Bom Jesus Brazil Universidade Estadual do Ceará UECE Fortaleza Brazil Universidade Federal de Vicosa Vicosa Brazil

This study aims to evaluate the possible allelopathic effects of young and mature leaf extract from of two eucalyptus genetic materials on the physiological performance of millet seeds. The experiment was carried out in a completely randomized design, in a 5 × 2 × 2 factorial arrangement [five concentrations of the extract (0, 25%, 50%, 75%, and 100%) × two genetic materials (1 - Eucalyptus grandis × Eucalyptus urophylla and 2 - Eucalyptus grandis × Eucalyptus camaldulensis) × two maturation stages of leaves (young and mature leaves)] with four replicates, each one with 25 seeds. Seeding was performed on the germitest paper substrate distributed in Petri dishes, moistened with the extract according to the treatments, being allocated in biochemical oxygen demand (B.O.D) adjusted at 25°C and 12 h photoperiod. Percentage, speed index, mean germination time, aerial part and primary root length, and dry matter weight of aerial part and root system of seedlings were evaluated. The leaf extracts of the two eucalyptus clones had all the analyzed variables changed with effect more evident on young leaf extract of the genetic material 1. It can be concluded that the tested genetic materials have a detrimental effect on the physiological performance of millet seeds.

关键词： Allelopathic Germination Pennisetum glaucum Seedlings Germination Speed

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Hypertension Associated with Atherosclerosis Risk Factors in Patients of Family Health Strategy Highlighting the Framingham Risk Score

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Open Journal of Preventive Medicine 2018年第8期8卷 229-247页

作者： bruna Lais da Silva Coutinho Rosana Andrade Brito Ana Isabel Cezario de Carvalho Conceicao Fabiola de Jesus Cardoso Andreza Silva dos Santos Bruno Felipe Ferreira Lopes Rubson Dantas da Silva Polyane Medeiros Alves Raiane dos Santos Pereira Alvaro Luis Muller da Fonseca Department of Education(DEDC7) Universidade do Estado da Bahia(UNEB)Senhor do BonfimBrazil Immunology Laboratory(LAIM) Department of Education(DEDC7)Universidade do Estado da Bahia(UNEB)Senhor do BonfimBrazil

The Systemic Arterial Hypertension (SAH) stands out among the chronic non-transmissible pathologies that impact the cause and/or aggravation of cardiovascular diseases (CVD) on a global level, as the disease is an underestimated disorder due to non-perceptive symptoms and associated with factors and risk markers of another CVD. Therefore, establishing the risk of progression and aggravation of the SAH, according the Framingham Risk Score (FRS), allows to reducing morbidity and improving preventative measures for DCVs. This observational and transversal study approaches the data collection of patient records at the Health Family Strategy of Senhor do bonfim, BA, which established differences by descriptive and inferential statistical analysis (correlation and regression). The aspects of hypertension associated with risk factors for atherosclerosis were analyzed, determining the risk of developing cardiovascular events in 10 years by FRS. From 432 families, 746 patients were selected, of which 340 are hypertensive individuals (SAH = 45.57%) and 406 (NSAH = 54.42%) non-hypertensives. Among the SAH the majority (31.17%) was in the age range of 63 - 77, but, in both groups, women were in stronger number. There was greater prevalence in SAH for all the characteristics analyzed, smoking (13.20%), sedentary (29.41%) and cardiovascular accident (22.60%). The SAH group is more susceptive to the CVD progress in 10 years by FRS (P < 0.0001 ANOVA). In the NSAH group, there were significant associations among all the variables analyzed as was expected, without differences between the linear correlation and regression, indicating the physio-metabolic equilibrium of the factors and markers evaluated by FRS. Already in SAH group, despite the correlations have been significant too, the regression analysis revealed that only Total Cholesterol (P = 0.0086);LDL (P < 0.0001), Glucose (P < 0.0006) and Age (P < 0.0001) have significative association with FRS. So, these factors and markers

关键词： Hypertension Risk Factors Atherosclerosis Cardiovascular Diseases Framingham Risk Score Family Health Strategy

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