检索结果-南通市图书馆

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第1期9卷 22-33页

作者： Si-yuan LI Jun-ling CAO Zhong-li SHI Jing-hong CHEN Zeng-tie ZHANG Clare E. HUGHES bruce.caterson Institute of Endemic Diseases College of Medicine Xi＇an Jiaotong University Key Laboratory of Environment and Genes Related to Diseases （Xi＇an Jiaotong University） Ministry of Education Kev Laboratory of Microelement and Endemic Disease （Xi＇an Jiaotong University） Ministr） of Health Xi＇an 710061 China Laboratory of Connective Tissue Biology School of Biosciences Cardiff University Cardiff CF10 3US UK

Objective: To identify the relationship between T-2 toxin and Kashin-Beck disease (KBD),the effects of T-2 toxin on aggrecan metabolism in human chondrocytes and cartilage were investigated in vitro. Methods: Chondrocytes were isolated from human articular cartilage and cultured in vitro. Hyaluronic acid (HA),soluble CD44 (sCD44),IL-1β and TNF-α levels in super-natants were measured by enzyme-linked immunosorbent assay (ELISA). CD44 content in chondrocyte membrane was deter-mined by flow cytometry (FCM). CD44,hyaluronic acid synthetase-2 (HAS-2) and aggrecanases mRNA levels in chondrocytes were determined using reverse transcription polymerase chain reaction (RT-PCR). Immunocytochemical method was used to investigate expressions of BC-13,3-B-3(-) and 2-B-6 epitopes in the cartilage reconstructed in vitro. Results: T-2 toxin inhibited CD44,HAS-2,and aggrecan mRNA expressions,but promoted aggrecanase-2 mRNA expression. Meanwhile,CD44 expression was found to be the lowest in the chondrocytes cultured with T-2 toxin and the highest in control plus selenium group. In addition,ELISA results indicated that there were higher sCD44,IL-1β and TNF-α levels in T-2 toxin group. Similarly,higher HA levels were also observed in T-2 toxin group using radioimmunoprecipitation assay (RIPA). Furthermore,using monoclonal antibodies BC-13,3-B-3 and 2-B-6,strong positive immunostaining was found in the reconstructed cartilage cultured with T-2 toxin,whereas no positive staining or very weak staining was observed in the cartilage cultured without T-2 toxin. Selenium could partly inhibit the effects of T-2 toxin above. Conclusion: T-2 toxin could inhibit aggrecan synthesis,promote aggrecanases and pro-inflammatory cytokines production,and consequently induce aggrecan degradation in chondrocytes. These will perturb metabolism balance between aggrecan synthesis and degradation in cartilage,inducing aggrecan loss in the end,which may be the initiation of the cartilage degradation.

关键词： T-2 toxin Kashin-Beck disease (KBD) Aggrecan IL-1β TNF-α Aggrecanase Hyaluronic acid (HA) CD44

Effects of moniliformin and selenium on human articular cartilage metabolism and their potential relationships to the pathogenesis of Kashin-Beck disease

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第3期11卷 200-208页

作者： An ZHANG Jun-ling CAO Bo YANG Jing-hong CHEN Zeng-tie ZHANG Si-yuan LI Qiang FU Clare E. HUGNES bruce.caterson Institute of Endemic Diseases School of Medicine Xi 'an Jiaotong University Xi 'an 710061 China Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education Xi 'an Jiaotong University Xi 'an 710061 China Key Laboratory of Microelement and Endemic Disease of Ministry of Health Xi 'an Jiaotong University Xi'an 710061 China Laboratory of Connective Tissue Biology School of Biosciences Cardiff University Cardiff CF10 3US UK

Objective： To investigate the effects of mycotoxin moniliformin （MON） on the metabolism of aggrecan and type 11 collagen in human chondrocytes in vitro and the relationship between MON and Kashin-Beck disease （KBD）. Methods： Human chondrocytes were isolated and cultured on bone matrix gelatin to form an artificial cartilage model in vitro with or without MON toxin. Cell viability was determined by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） assay. The expression of aggrecan and type II collagen in the cartilage was determined using immunocytochemical staining. Results： MON toxin inhibited chondrocyte viability in dose-dependent and time-dependent manners. MON reduced aggrecan and type Ⅱ collagen syntheses in the tissue-engineered cartilage. MON also increased the expression of matrix metalloproteinase-1 （MMP-1）, MMP-13, BC4 epitopes, and CD44 in cartilages. However, the expression of 3B3（-） epitopes in cartilages was inhibited by MON. Selenium partially alleviated the damage of aggrecan induced by MON toxin. Conclusion： MON toxin promoted the catabolism of aggrecan and type II collagen in human chondrocytes.

关键词： Chondrocytes Moniliformin Selenium Aggrecan Collagen Matrix metalloproteinases (MMPs) CD44 Kashin-Beck disease

The Etiology of Kashin-Beck Disease (KBD):The Effects of Fungal Mycotoxins on Chondrocyte and Cartilage Matrix Metabolism

在线全文

学校读者我要写书评

暂无评论

The Etiology of Kashin-Beck Disease (KBD):The Effects of Fun...

The effect of Deoxynivalenol,Nivalenol,T-2 toxin and Selenium on the metabolism of tissue engineered cartilage in vitro

2008年全国生物化学与分子生物学学术大会

作者： Clare E.Hughes bruce.caterson School of Biosciences Cardiff UniversityCardiff UK

Purpose:KBD is an endemic osteoarthropathy which is mainly characterised by the degeneration and necrosis of hyaline articular *** present the etiology and pathogenesis of KBD is *** aim of this study was to investiga...

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

The effect of Deoxynivalenol,Nivalenol,T-2 toxin and Seleniu...

Fracturing,caving propagation and influence of mining on groundwater above longwall panels–a review of predictive models

中国环境诱变剂学会第14届学术交流会议

作者： Clare E.Hughes bruce.caterson Laboratory of Connective Tissue Biology School of BiosciencesCardiff UniversityCardiffCF10 3USUK

Purpose:To investigate the effects of Nivalenol(NIV),Deoxynivalenol(DON) and T-2 toxin,in the presence and absence of Selenium(Se),on the metabolism of tissue engineered *** in vitro cartilage cultures mimic those found in Kashin-Beck Disease(KBD) environments that cause diarthrodial joint degeneration in development and ***:Bone matrix gelatin(BMG) was prepared from cancellous bones of adult

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

International Journal of Mining Science and Technology 2020年第1期30卷 49-54页

作者： bruce Hebblewhite School of Minerals and Energy Resources Engineering University of New South WalesSydney 2052Australia

Historically there have been a number of different hypotheses and empirical models developed in an attempt to describe the nature of fracturing above longwall panels in underground coal *** motivation for suchmodels varies,ranging fromunderstanding the impact ofmining on surface subsidence,to back-analysis of caving behaviour in the immediate roof behind the longwall *** of the most critical motivating factors that is taking on increased importance in many coalfields,is the need for better understanding,and hence prediction of the impact of mining on overlying strata,particularly strata units acting as aquifers for different groundwater *** paper reviews some of the major prediction models in the context of observed behaviour of strata displacement and fracturing above longwall panels in the southern coalfields of New South Wales,south of *** paper discusses the parameter often referred to as"height of fracturing"in terms of the critical parameters that influence it,and the relevance and appropriateness of this terminology in the context of overlying sub-surface subsidence and groundwater *** paper proposes an alternative terminology for this parameter that better reflects what it is and how it is *** paper also addresses the potential role of major bedding shear planes mobilised by mining and their potential influence on overlying subsidence and groundwater interference.

关键词： Longwall mining Subsidence Groundwater Caving Underground coal mining

Study of Electron Beam Surfi-sculpt during Composite Materials Joining

在线全文

学校读者我要写书评

暂无评论

稀有金属材料与工程 2011年第S4期40卷 292-296页

作者： bruce Dance The Welding Institute(TWI) Cambridge CB1 6ALUK

Electron beam surfi-sculpt is a new metal surface processing technology,which is widely used in the areas of materials surface treatment,composite materials manufacturing,and so *** this paper,comeld technology,which was an application technology of electron beam surfi-sculpt during composite materials area,was *** tensile experiments results and failure forms of Ti6Al4V/Carbon fibre single step joints were compared with different joining methods,such as slick embedding,adhesive bonding,riveting and *** was found that comeld joint had more advantage of tensile property than other joining *** the same time,tensile process of comeld joint was analyzed with numerical simulation,and key factors of protrusions influenced tensile property were obtained,which was of importance to structure design and joint property improvement.

关键词： electron beam surfi-sculpt comeld joining methods tensile experiments numerical simulation

Increased expression of chondroitin sulphate proteoglycans in rat hepatocellular carcinoma tissues

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2012年第30期18卷 3962-3976页

作者： Xiao-Li Jia Si-Yuan Li Shuang-Suo Dang Yan-An Cheng Xin Zhang Wen-Jun Wang Clare E Hughes bruce.caterson Department of Infectious Diseases The Second Affiliated Hospital of Medical School of Xi'an Jiaotong University Xi'an 710004 Shaanxi Province China Connective Tissue Biology Laboratories Division of Pathophysiology and Repair School of Biosciences Cardiff University Cardiff Wales CF10 3AX United Kingdom The Institute of Endemic Disease Medical School of Xi'an Jiaotong University Xi'an 710061 Shaanxi Province China

AIM:To investigate the expression of chondroitin sulphate proteoglycans(CSPGs)in rat liver tissues of hepatocellular carcinoma(HCC).METHODS:Thirty male Sprague Dawley rats were randomly divided into two groups:control group(n=10) and HCC model group(n=20).Rats in the HCC model groups were intragastrically administrated with 0.2%(w/v)N-diethylnitrosamine(DEN)every 5 d for 16 wk,whereas 0.9%(w/v)normal saline was administered to rats in the control *** 16 wk from the initiation of experiment,all rats were killed and livers were collected and fixed in 4%(w/v)*** tissues were embedded in paraffin and *** staining(hematoxylin and eosin and Toluidine blue)was performed to demonstrate the onset of HCC and the content of sulphated glycosaminoglycan(sGAG).Immunohistochemical staining was performed to investigate the expression of chondroitin sulphate(CS)/dermatan sulphate(DS)-GAG,heparan sulphate(HS)-GAG,keratan sulphate(KS)-GAG in liver ***,expression and distribution of CSPG family members,including aggrecan,versican,biglycan and decorin in liver tissues,were also immunohistochemically ***:After 16 wk administration of DEN,malignant nodules were observed on the surface of livers from the HCC model group,and their hepatic lobule structures appeared largely disrupted under *** blue staining demonstrated that there was an significant increase in sGAG content in HCC tissues when compared with that in the normal liver tissues from the control group[0.37±0.05 integrated optical density per stained area(IOD/area)and 0.21± 0.01 IOD/area,P<0.05].Immunohistochemical studies demonstrated that this increased sGAG in HCC tissues was induced by an elevated expression of CS/DS(0.28±0.02 IOD/area and 0.18±0.02 IOD/area,P< 0.05)and HS(0.30±0.03 IOD/area and 0.17±0.02 IOD/area,P<0.01)but not KS GAGs in HCC *** studies thereby were performed to investigate the expression and distribution o

关键词： Hepatocellular carcinoma Proteoglycan Chondroitin sulphate Heparan sulphate Keratan sulphate

A review of the geomechanics aspects of a double fatality coal burst at Austar Colliery in NSW,Australia in April 2014

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Mining Science and Technology 2017年第1期27卷 3-7页

作者： Hebblewhite bruce Calvin Jim School of Mining Engineering UNSWSydney 2052Australia

A coal burst occurred on 15 April, 2014 at the Austar Coal Mine, located west of Newcastle, NSW,Australia. The burst resulted in fatal injuries to two men working as part of the mining crew at the development face. At the time, a continuous miner was being used to mine a longwall development gate road through heavily structured coal, at a depth of approximately 550 m. A number of pre-cursor bumps had occurred on previous shifts, emanating from the coal ribs of the roadway, in proximity to the coal *** paper reviews the geological, geotechnical and mining conditions and circumstances leading up to the coal burst event; and presents and discusses the available evidence and possible interpretations relating to the geomechanical behaviour mechanisms that may have been critical factors in this incident. The paper also discusses some key technical and operational considerations of ground support systems and mining practices and strategies needed for operating in such conditions in the future.

关键词： Underground coal mining Roadway development Strata controlCoal burst Coal geology Mine safety

Biology Past and Biology Future:Where have we been and where are we going?

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2013年第25期8卷 2309-2316页

作者： bruce Alberts Department of Biochemistry and Biophysics the University of California San Francisco

bruce Alberts, a prominent biochemist with a strong commitment to the improvement of science and mathematics education, serves as Editor-in-Chief of Science and as one of President Obama＇s first three Science Envoys. Alberts is also Professor Emeritus in the Department of Biochemistry and Biophysics at the University of California, San Francisco, to which he returned after serving two six-year terms as the president of the National Academy of Sciences （NAS）.

关键词： Biology Past and Biology Future