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Establishment and characterization of two new human embryonic stem cell lines,SYSU-1 and SYSU-2

Chinese Medical Journal 2007年第7期120卷 589-594页

作者： HUANG Guo LI Wei-qiang CHEN Rui CHEN Zhen-guang ZHANG Xiu-ming MAO Fu-xiang HUANG Shao-liang LI Shu-nong bruce t.lahn Andy Peng Xiang Centre for Stem Cell Biology and tissue Engineering Sun Yat-sen University Guangzhou 510080 China Fourth People＇s Hospital of Shenzhen Shenzhen 518033 China Department of thoracic Surgery First Affiliated Hospital Sun Yat-sen University Guangzhou 510080 China

Background Human embryonic stem cells can propagate indefinitely in vitro and are able to differentiate into derivatives of all three embryonic germ layers. the excitement surrounding human embryonic stem cells lies largely in their potential to produce specialized cells that can be used for transplant therapies. However, further investigation requires additional cell lines with varying genetic background. therefore, efforts to derive and establish more human embryonic stern cell lines are highly warranted. Methods Surplus embryos （blastocysts） from donors were used to isolate the inner cell mass by immunosurgery. All cells were cultured continuously on irradiated murine embryonic fibroblasts feed layer and likely human embryonic stem cell colonies were subsequently characterized by cell surface marker staining, karyotyping and teratoma formation. Results two human embryonic stern cell lines （SYSU-1 and SYSU-2） were established from surplus embryos. the two lines express several pluripotency markers including alkaline phosphatase, SSEA- 4, tra-1-60, Oct-4, Nanog and Rex-1. they remain in undifferentiated state with normal karyotype after prolonged passages and can form embryoid bodies in vitro and teratoma in vivo. Conclusion two new human embryonic stem cell lines have been established from surplus embryos. they can be used to understand selfrenewal and differentiating mechanisms and provide more choices for regenerative medicine.

关键词： embryonic stem cells blastocysts inner cell mass cell differentiation

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Assessment of embryotoxicity of compounds in cosmetics by the embryonic stem cell test

Assessment of embryotoxicity of compounds in cosmetics by th...

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第八届中国化妆品学术研讨会

作者： bruce t.lahn Andy Peng Xiang Center for Stem Cell Biology and tissue Engineering Sun Yat-sen University

the new EU legislations for cosmetics(Seventh Amendment) have laid down deadlines for the replacement of animal tests in cosmetics.this policy stimulates the acceptance of in vitro approaches to test embryotoxic potentials of compounds in cosmetics products.the embryonic stem cell test(ESt) designed by the European Centre for the Validation of Alternative Methods(ECVAM) is currently the most promising in vitro assay to predict the embryotoxic potential of compounds.In this study,six selected compounds (hydroquinone,eugenol,dibutyl phthalate,antimony(Ⅲ) oxide,neodymium(Ⅲ) nitrate hydrate,melamine) formerly involved in cosmetic products were selected to test their embryotoxic potentials by the ESt. 5-Fluorouracil and penicillin G were separately set as positive and negative control.the embryotoxic potential was determined by the prediction model(PM),which was calculated from three endpoints,the IC50 3t3,IC50 ES,and ID50.Hydroquinone,eugenol,and antimony(Ⅲ) oxide indicated with strong embryotoxicity,while dibutyl phthalate,neodymium(Ⅲ) nitrate hydrate,and melamine exhibited a weak embryotoxicity.these results may provide a valuable attempt to expand the application of ESt to the cosmetics field.

关键词： Cosmetics embryotoxicity embryonic stem cell test

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Nestin is a critical prosurvival factor in neural stem/progenitor cells

Nestin is a critical prosurvival factor in neural stem/proge...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Donghyun Park Andy Peng Xiang Frank Fuxiang Mao Noah Walton bruce t.lahn Howard Hughes Medical Institute Department of Human GeneticsUniversity of ChicagoChicago IL 60637USA Center for Stem Cell Biology and tissue Engineering Sun Yat-sen University

＜正＞the intermediate filament protein,nestin,is a widely employed marker of multipotent neural stem/progenitor cells（NSCs）.Recent in vitro studies have implicated nestin in a number of cellular processes,but there is no evidence yet on its in vivo function.Here,we report the construction and functional characterization of Nestin knockout mice.We found mat these mice show embryonic lethality,with neuroepithelia of the developing neural tube exhibiting significantly increased apoptosis.Consistent with this in vivo observation,NSC cultures derived from knockout embryos show dramatically elevated apoptosis but no defects in proliferation or differentiation.Unexpectedly,nestin deficiency has no deflectable effect on the integrity of the cytoskeleton.Furthermore,the knockout of vimentin,while abolishing nestin’s ability to polymerize into intermediate filaments in NSCs,does not lead to any apoptotic phenotype.these data demonstrate that nestin is a critical prosurvival factor in NSCs,and that nestin’s prosurvival function is surprisingly uncoupled from its structural involvement in the cytoskeleton.

关键词： nestin intermediate filament apoptosis neural stem cells neural progenitor cells vimentin

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韩国生活用纸行业——依赖进口,专注维持生产技术能力

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生活用纸 2024年第3期24卷 38-41页

作者： bruce Janda 不详

韩国作为一个出口驱动型经济强国,其生活用纸行业是个罕见的例外,为净进口。但是,相比大多数发达经济体,韩国受到失业率和通货膨胀的影响较小,没有太多阻碍消费增长的问题,因此,生产商正在集中精力提高技术能力,而不是扩大规模。2023—2... 详细信息

韩国作为一个出口驱动型经济强国,其生活用纸行业是个罕见的例外,为净进口。但是,相比大多数发达经济体,韩国受到失业率和通货膨胀的影响较小,没有太多阻碍消费增长的问题,因此,生产商正在集中精力提高技术能力,而不是扩大规模。2023—2028年期间,韩国纸浆和纸板市场预计将以5.20%的复合年均增长率增长。一个重要因素是当地消费者对环保产品和包装的认识不断增强。2020年,韩国的纸张产量大约为1,163万t。

关键词：发达经济体失业率环保产品经济强国净进口通货膨胀生产技术能力年均增长率

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Prophylaxis with intraocular pressure lowering medication and glaucomatous progression in patients receiving intravitreal anti-VEGF therapy

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International Journal of Ophthalmology(English edition) 2022年第10期15卷 1611-1618页

作者： Jeanette Du James t Patrie Xiao-Yu Cai bruce E Prum Yevgeniy Shildkrot New York Eye and Ear Infirmary of Mount Sinai 310 East 14 StreetNew YorkNY 10003USA Department of Public Health Sciences University of VirginiaP.0.Box 800717CharlottesvilleVirginia 22908USA Department of Ophthalmology University of Virginia School of Medicine1300 Jefferson Park AveCharlottesvilleVirginia 22908USA

AIM: to investigate whether pretreatment with pressurelowering medication prior to anti-vascular endothelial factor(VEGF) injections had an effect on glaucomatous progression in patients with preexisting glaucoma or ocular hypertension(OHt).MEtHODS: A total of 66 eyes from 54 patients with a preexisting diagnosis of glaucoma or OHt, treated with six or more anti-VEGF injections were selected for chart review. Primary outcome measures were rate of visual field loss in d B/year, rate of change in retinal nerve fiber layer(RNFL) thickness in microns/year, and need for additional glaucoma intervention.RESULtS: the number of eyes requiring additional glaucoma medication was 5 of 20(25.0%) and 14 of 46(30.4%) for the pretreated and non-pretreated groups, respectively. the number of eyes requiring glaucoma laser or surgery was 4 of 20(20.0%) and 13 of 46(28.3%) for the pretreated and non-pretreated groups, respectively. Estimated mean rate of pattern standard deviation decline was not significant in either group(P>0.073), with no difference between groups(P=0.332). Although both groups showed significant RNFL change from baseline(Ptected between groups(P=0.467).CONCLUSION: Pretreatment has no detectable effect on structural or functional glaucomatous progression. Patients receiving repeated injections may be at risk for glaucomatous complications requiring invasive intervention.

关键词： anti-vascular endothelial growth factor therapy pretreatment glaucomatous progression

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Contemporary,national patterns of surgery after preoperative therapy for stageⅡ/Ⅲrectal adenocarcinoma

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World Journal of Gastrointestinal Oncology 2022年第6期14卷 1148-1161页

作者： Celine Soriano Henry t Bahnson Jennifer A Kaplan bruce Lin Ravi Moonka Huong t Pham Hagen F Kennecke Vlad Simianu Department of Surgery Virginia Mason Franciscan HealthSeattleWA 98101United States Benaroya Research Institute SeattleWA 98101United States Department of Hematology Oncology Virginia Mason Franciscan HealthSeattleWA 98101United States Department of Radiation Oncology Virginia Mason Franciscan HealthSeattleWA 98101United States Department of Medical Oncology Providence Cancer InstititutePortlandOR 97213United States Section of Colon and Rectal Surgery Department of SurgeryVirginia Mason Medical CenterSeattleWA 98101United States

BACKGROUND Contemporary treatment of stageⅡ/Ⅲrectal cancer combines chemotherapy,chemoradiation,and surgery,though the sequence of surgery with neoadjuvant treatments and benefits of minimally-invasive surgery(MIS)is debated.AIM to describe patterns of surgical approach for stageⅡ/Ⅲrectal cancer in relation to neoadjuvant therapies.MEtHODS A retrospective cohort was created using the National Cancer Database.Primary outcome was rate of sphincter-sparing surgery after neoadjuvant therapy.Secondary outcomes were surgical approach(open,laparoscopic,or robotic),surgical quality(R0 resection and 12+lymph nodes),and overall survival.RESULtS A total of 38927 patients with clinical stageⅡorⅢrectal adenocarcinoma underwent surgical resection from 2010-2016.Clinical stageⅡpatients had neoadjuvant chemoradiation less frequently compared to stageⅢ(75.8%vs 84.7%,Pt had similar rates of total neoadjuvant therapy(tNt)(27.0%vs 27.2%,P=0.697).Overall rates of total mesorectal excision without sphincter preservation were similar between clinical stageⅡandⅢ(30.0%vs 30.3%)and similar if preoperative treatment was chemoradiation(31.3%)or tNt(30.2%).Over the study period,proportion of cases approached laparoscopically increased from 24.9%to 32.5%and robotically 5.6%to 30.7%(Pthis cohort showed improved survival for MIS approaches compared to open surgery(laparoscopy HR 0.85,95%CI 0.78-0.93,and robotic HR 0.82,95%CI 0.73-0.92).CONCLUSION Sphincter preservation rates are similar across stageⅡandⅢrectal cancer,regardless of delivery of preoperative chemotherapy,chemoradiation,or both.At a national level,there is a shift to predominantly MIS approaches for rectal cancer,regardless of whether sphincter sparing procedure is performed.

关键词： Rectal cancer total neoadjuvant therapy Colorectal surgery Minimally-invasive surgery Chemotherapy Radiation

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Moving towards the chemo-free treatment of lymphoma:hype or reality?

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Journal of Cancer Metastasis and treatment 2022年第1期8卷 631-636页

作者： bruce D.Cheson Scientific Advisor Lymphoma Research FoundationNew YorkNY 10005USA

A new generation of novel,effective targeted drugs and cellular therapies include monoclonal antibodies directed at the cell surface,such as the anti-CD-19 tafasitamab which,combined with lenalidomide,is the first therapy approved by the Food and Drug Administration for second-line treatment of diffuse large B-cell lymphoma.Other agents interfere with pro-survival intracellular signaling pathways including drugs that inhibit Bruton tyrosine kinase,phosphatidylinositol-3 kinase(PI3-kinase),and bcl-2.An increasing number of therapies impact the microenvironment,notably checkpoint inhibitors and bispecific antibodies.Chimeric antigen receptor-t cell therapy has improved the outcome of patients with a variety of histologies of lymphoma.Whereas in the past,such therapies would be used inrelapsed and refractory settings,they are now being evaluated as initial treatment in selected patients.With an improved ability to individualize treatment approaches,chemo-free will be a reality for lymphoma patients.

关键词： Lymphoma targeted therapies chemo-free bispecifics BtK inhibitors PI3k inhibitors CAR-t cell

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A Systematic Review of the Genetic Predisposition to Injury in Football

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Journal of Science in Sport and Exercise 2023年第2期5卷 97-115页

作者： Alexander B.t.McAuley David C.Hughes Loukia G.tsaprouni Ian Varley bruce Suraci thomas R.Roos Adam J.Herbert Daniel t.Jackson Adam L.Kelly Faculty of Health Education and Life SciencesBirmingham City UniversityBirminghamWest MidlandsUK Department of Sport Science Nottingham Trent UniversityNottinghamUK Academy Coaching Department AFC BournemouthBournemouthUK the International Academy of Sports Science and technology(AIStS) University of LausanneLausanneSwitzerland Department of Life Sciences Birmingham City UniversityCity South CampusWestbourne RoadEdgbaston B153TNUK

Purpose Synthesise genetic association studies investigating injury involving football players to identify which genetic variants have the most empirical evidence to date.Methods A comprehensive search of the PubMed,SPORtDiscus,and MEDLINE databases until March 11th 2022 identi-fied 34 studies.Inclusion criteria:primary investigations,included football players,examined the association of a genetic variant with injury,and were published in English.Risk of bias was assessed using the Newcastle–Ottawa Scale.A narrative synthesis summarised results.Results there were 33 candidate gene studies and one genome-wide study,with 9642 participants across all studies(range=43–1311;median=227).Ninety-nine polymorphisms were assessed within 63 genes.Forty-one polymorphisms were associated with injury once.three polymorphisms had their specific allelic associations with injury replicated twice in independent cohorts:ACtN3(rs1815739)XX genotype was associated with an increased susceptibility to non-contact muscle injuries,ACAN(rs1516797)G allele was associated with increased susceptibility to anterior cruciate ligament(ACL)injuries,and VEGFA(rs2010963)CC genotype was associated with an increased susceptibility to ACL and ligament or tendon injuries.However,several methodological issues(e.g.,small sample sizes,cohort heterogeneity,and population stratification)are prevalent that limit the reliability and external validity of findings.Conclusion At present,the evidence base supporting the integration of genetic information as a prognostic or diagnosis tool for injury risk in football is weak.Future participation of organisations in international consortia is suggested to combat the current methodological issues and subsequently improve clarity concerning the underlying genetic contribution to injury susceptibility.

关键词： Soccer Genomics Polymorphism Snp Susceptibility

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Wind farm potential is higher in prime habitat for uncommon soil crust lichens

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Ecological Processes 2013年第1期2卷 108-115页

作者： Heather t Root Myrica McCune bruce McCune Department of Botany and Plant Pathology Oregon State University2082 Cordley HallCorvallisOR 97330USA Institute for Natural Resources Oregon State University210 Strand Agricultural HallCorvallisOR97331USA Department of Forest Ecosystems and Society Oregon State University321 Richardson HallCorvallisOR97331USA

Introduction:Biotic soil crust communities contribute valuable ecosystem services and biodiversity in steppe ecosystems.the uncommon crust lichens Acarospora schleicheri,Fuscopannaria cyanolepra,Rhizocarpon diploschistidina,and texosporium sancti-jacobi are associated with fine-textured soils along rivers of the Columbia Basin.A.schleicheri and R.diploschistidina indicate late-successional habitat and may serve as indicators for other rare or cryptic species associated with similar habitats.Much of the most favorable habitat for these species has been lost to urban and agricultural development.We sought to overlay favorable habitats with wind farm development potential to assess whether these species are likely to be affected by renewable energy development.Methods:We overlaid habitat models for four lichen species on land use and wind farm potential maps.Using a sample of 5,000 points,we determined whether there were differences in probability of occurrence among wind farm potential classes within developed and natural lands using Multi-Response Permutation Procedures.Sites with modeled probability of occurrence greater than 60%were considered“favorable”habitats;for these,aχ2 test allowed us to determine whether favorable habitats were associated with wind farm potential categories.Results:Sites that are developed for agriculture or have higher wind farm potential coincide with more favorable habitats for uncommon soil crust lichens.Of the favorable habitats for the four focal lichens,28–42%are already affected by development or agriculture;5–14%of favorable habitats remain in natural vegetation and are considered sites with fair or good potential for wind farms.Conclusions:Development of wind energy has the potential to negatively impact uncommon soil crust lichen species because favorable sites coincide with especially good habitat for these species.However,as these renewable energy resources are developed,we have the opportunity to ensure that valuable soil crust functions and diversity are maintained by surv

关键词： Acarospora schleicheri Agriculture Biotic soil crust Columbia river Fuscopannaria cyanolepra Land use Rhizocarpon diploschistidina Steppe texosporium sancti-jacobi

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Physiology of in-situ arterial revascularization in coronary artery bypass grafting: Preoperative,intraoperative and postoperative factors and influences

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World Journal of Cardiology 2016年第11期8卷 623-637页

作者： t bruce Ferguson Jr CV Sciences East Carolina Heart InstituteEast Carolina Diabetes and Obesity InstituteBrody School of Medicine at East Carolina University

Surgical revascularization with coronary artery bypass grafting(CABG) has become established as the most effective interventional therapy for patients with moderately severe and severe stable ischemic heart disease(SIHD). this recommendation is based on traditional 5-year outcomes of mortality and avoidance of myocardial infarction leading to reintervention and/or cardiac death. However, these results are confounded in that they challenge the traditional CABG surgical tenets of completeness of anatomic revascularization, the impact of arterial revascularization on late survival, and the lesser impact of secondary prevention following CABG on late outcomes. Moreover, the emergence of physiologic-based revascularization with percutaneous cardiovascular intervention as an alternative strategy for revascularization in SIHD raises the question of whether there are similar physiologic effects in CABG. Finally, the ongoing ISCHEMIA trial is specifically addressing the importance of the physiology of moderate or severe ischemia in optimizing therapeutic interventions in SIHD. So it is time to address the role that physiology plays in surgical revascularization. the long-standing anatomic framework for surgical revascularization is no longer sufficient to explain the mechanisms for short-term and long-term outcomes in CABG. Novel intraoperative imaging technologies have generated important new data on the physiologic blood flow and myocardial perfusion responses to revascularization on an individual graft and global basis. Long-standing assumptions about technical issues such as competitive flow are brought into question by real-time visualization of the physiology of revascularization. Our underestimation of the impact of Guideline Directed Medical therapy, or Optimal Medical therapy, on the physiology of preoperative SIHD, and the full impact of secondary prevention on post-intervention SIHD, must be better understood. In this review, these issues are addressed through the perspective of multi-arterial revascularization

关键词：冠的动脉绕过 grafting 动脉的 revascularization 心肌的灌注外科的结果 Intraoperative 成像

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