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Effects of selective estrogen receptor agonists on estrogen receptor expression in the uterus of ovariectomized rats

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Open Journal of Molecular and Integrative Physiology 2012年第2期2卷 35-43页

作者： C. S. Blesson britt masironi Lena Sahlin Division for Reproductive Endocrinology and the Pediatric Endocrinology Unit Department of Women’s and Children’s Health Karolinska Institutet Stockholm Sweden

The aim of the present study was to investigate effects of short time treatment with estrogen receptor (ER) selective agonists on ERα, ERβ and G-protein coupled estrogen receptor-1 (GPER) expression in the uterus of ovariectomized rats. The rats were treated with either estradiol (E2), the specific ERα agonist PPT or the specific ERβ agonist DPN for 18 hrs. Uterine weights were higher after E2 or PPT treatment than after DPN or no treatment. ERα mRNA levels decreased significantly in PPT and DPN treated animals as compared to controls. Stromal ERα immunostaining was higher after E2 treatment than in controls. The ERβ mRNA level was lower in the E2 and PPT groups compared with controls. ERβ immunoreactivity was higher in the myometrium after DPN treatment than in controls. The GPER mRNA level was lower in the E2 and DPN groups as compared to the controls, whereas total protein levels did not display any change. The proliferation marker Ki-67 increased after PPT treatment in stroma and myometrium, as compared to controls. Thus, uterine growth and proliferation are predominately regulated via ERα. Also ERβ expression showed regulation via ERα, while GPER expression indicated control via ERβ. This short term treatment did not result in any regulation of the total protein level as determined by Western blot. However, treatment by E2 increased ERα immunostaining in the stroma and DPN augmented ERβ immunostaining in the myometrium. Thus, the estrogen receptors in the rat uterus are differently regulated depending on the ligand and tissue type.

关键词： PPT DPN ERα ERβ GPER

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Expression and localization of prostaglandin receptors and stromal factors in human cervix—Variations in pregnant and non-pregnant states

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Open Journal of Molecular and Integrative Physiology 2013年第4期3卷 147-157页

作者： Chellakkan S. Blesson Nathalie Roos Olof Stephansson britt masironi Susann Reinert Ylva Vladic Stjernholm Gunvor Ekman-Ordeberg Lena Sahlin Division for Reproductive Endocrinology and the Pediatric Endocrinology Unit Department of Women’s and Children’s Health Karolinska University Hospital-Solna Karolinska Institutet Stockholm Sweden Division for Reproductive Endocrinology and the Pediatric Endocrinology Unit Department of Women’s and Children’s Health Karolinska University Hospital-Solna Karolinska Institutet Stockholm Sweden Division of Obstetrics and Gynecology Department of Women’s and Children’s Health Karolinska University Hospital-Solna Karolinska Institutet Stockholm Sweden Division of Obstetrics and Gynecology Department of Women’s and Children’s Health Karolinska University Hospital-Solna Karolinska Institutet Stockholm Sweden Division of Obstetrics and Gynecology Department of Women’s and Children’s Health Karolinska University Hospital-Solna Karolinska Institutet Stockholm Sweden Clinical Epidemiology Unit Department of Medicine Karolinska Institutet Stockholm Sweden

Prostaglandins (PGs) mediate cervical ripening leading to parturition. PGs are used successfully to induce cervical ripening. However, the cell type specific expression of PG receptor subtypes and various stromal factors important for cervical ripening in human cervix is not known. Our objective was to investigate the expression and localization of PG receptors EP1-4 and FP and localization of stromal factors CTGF (connective tissue growth factor), furin, calgranulin ?B, ALOX12 (arachidonate 12-lipooxy-genase) and ALOX15 in human cervical tissue from pregnant and non-pregnant women. Cervical biopsies were obtained from non-pregnant (NP), term pregnant (TP) and post-partum (PP) women. The mRNA expression was determined with real-time PCR, protein expression and localization with immunohistochemistry. Our results show that the EP2 mRNA level was higher in the PP group as compared to TP, whereas the EP4 mRNA level was lower in the TP group as compared to NP. Concomitantly stromal EP2 and epithelial EP3 immunoreactivity was higher in the TP as compared to the NP group, while the EP4 immunostaining in glands was lower in the TP as compared to the PP group. Immunostaining of endothelial CTGF, smooth muscle furin and ALOX12, were all lower in the TP group as compared to NP, for CTGF also the PP group was lower than NP. Endothelial calgranulin B immunoreactivity was higher in the PP group than the NP group. PG receptors and stromal factors exhibit differential expression in the cervix from women in non-pregnant and pregnant states, implying their involvement in the process of cervical ripening.

关键词： Cervical Ripening Pregnancy Extracellular Matrix Prostaglandin Receptors

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Techno-economical evaluation of membrane based biogas upgrading system:A comparison between polymeric membrane and carbon membrane technology

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Green Energy & Environment 2016年第3期1卷 222-234页

作者： Shamim Haider Arne Lindbrathen May-britt Hagg Norwegian University of Science and Technology NTNU Department of Chemical Engineering

A shift to renewable energy sources will reduce emissions of greenhouse gases and secure future energy supplies. In this context, utilization of biogas will play a prominent role. Focus of this work is upgrading of biogas to fuel quality by membrane separation using a carbon hollow fibre(CHF) membrane and compare with a commercially available polymeric membrane(polyimide) through economical assessment. CHF membrane modules were prepared for pilot plant testing and performance measured using CO_2, O_2, N_2. The CHF membrane was modified through oxidation, chemical vapour deposition(CVD) and reduction process thus tailoring pores for separation and increased performance. The post oxidized and reduced carbon hollow fibres(PORCHFs) significantly exceeded CHF performance showing higher CO_2 permeance(0.021 m^3(STP)/m^2 h bar) and CO_2/CH_4 selectivity of 246(5 bar feed vs 50 mbar permeate pressure). The highest performance recorded through experiments(CHF and PORCHF) was used as simulation basis. A membrane simulation model was used and interfaced to 8.6 V Aspen HYSYS.A 300 Nm^3/h mixture of CO_2/CH_4 containing 30-50% CO_2 at feed pressures 6, 8 and 10 bar, was simulated and process designed to recover99.5% CH_4 with 97.5% purity. Net present value(NPV) was calculated for base case and optimal pressure(50 bar for CHF and PORCHF). The results indicated that recycle ratio(recycle/feed) ranged from 0.2 to 10, specific energy from 0.15 to 0.8(kW/Nm^3 feed) and specific membrane area from 45 to 4700(m^2/Nm^3 feed). The high recycle ratio can create problems during start-up, as it would take long to adjust volumetric flow ratio towards 10. The best membrane separation system employs a three-stage system with polyimide at 10 bar, and a two-stage membrane system with PORCHF membranes at 50 bar with recycle. Considering biomethane price of 0.78 $/Nm^3 and a lifetime of 15 years, the technoeconomic analysis showed that payback time for the best cascade is 1.6 months.

关键词： Carbon membrane Biogas upgrading Techno-economical analysis NPV calculations

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Numerical Simulation of Time-Harmonic Waves in Inhomogeneous Media using Compact High Order Schemes

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Communications in Computational Physics 2011年第3期9卷 520-541页

作者： Steven britt Semyon Tsynkov Eli Turkel Department of Mathematics North Carolina State UniversityBox 8205RaleighNC 27695USA School of Mathematical Sciences Tel Aviv UniversityRamat AvivTel Aviv 69978Israel

In many problems,one wishes to solve the Helmholtz equation with variable coefficients within the Laplacian-like term and use a high order accurate method(e.g.,fourth order accurate)to alleviate the points-per-wavelength constraint by reducing the dispersion *** variation of coefficients in the equation may be due to an inhomogeneous medium and/or non-Cartesian *** renders existing fourth order finite difference methods *** develop a new compact scheme that is provably fourth order accurate even for these *** present numerical results that corroborate the fourth order convergence rate for several model problems.

关键词： Helmholtz equation variable coefficients high order accuracy compact finite differences

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Synthesis and properties of SrFe_(12)O_(19) obtained by solid waste recycling of oily cold rolling mill sludge

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International Journal of Minerals,Metallurgy and Materials 2019年第5期26卷 642-648页

作者： Bo Liu Shen-gen Zhang britt-Marie Steenari Christian Ekberg Institute for Advanced Materials and Technology University of Science and Technology Beijing Nuclear Chemistry and Industrial Material Recycling Department of Chemistry and Chemical Engineering Chalmers University of Technology

The aim of this study was to develop a new approach for the preparation of environmentally friendly, high-value products from oily cold rolling mill(CRM) sludge. Utilizing oily CRM sludge as a source of iron, strontium hexaferrite(SrFe_(12)O_(19)) powders were prepared by multi-step processes involving acid leaching, chemical conversion treatment, and synthesis by a citrate precursor. The influences of citric acid dosage and the pH of the sol system on the formation, crystallite size, and magnetic properties of the obtained SrFe_(12)O_(19) powders were investigated. High saturation magnetization(74.8 mA·m^2·g^(–1)) and intrinsic coercivities(614.46 mT) were achieved for pH 7.0 of the sol system, for which the molar ratio of citric acid dosage to the total dosage of Fe^(3+) and Sr^(2+) was 1.5. This study presents a new approach to utilizing oily CRM sludge, and even refractory iron-containing solid waste.

关键词： strontium ferrite oily cold rolling mill sludge magnetic properties citrate precursor method recycling

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FunHoP:Enhanced Visualization and Analysis of Functionally Homologous Proteins in Complex Metabolic Networks

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Genomics, Proteomics & Bioinformatics 2021年第5期19卷 848-859页

作者： Kjersti Rise May-britt Tessem Finn Drablos Morten B.Rye Department of Clinical and Molecular Medicine NTNU-Norwegian University of Science and TechnologyTrondheim NO-7491Norway Department of Circulation and Medical Imaging NTNU-Norwegian University of Science and TechnologyTrondheim NO-7491Norway Clinic of Surgery St.Olavs HospitalTrondheim University HospitalTrondheim NO-7491Norway

Cytoscape is often used for visualization and analysis of metabolic *** example,based on KEGG data,a reader for KEGG Markup Language(KGML)is used to load files into ***,although multiple genes can be responsible for the same reaction,the KGMLreader KEGGScape only presents the first listed gene in a network node for a given *** can lead to incorrect interpretations of the *** new method,FunHoP,shows all possible genes in each node,making the pathways more *** collapses all genes in a node into one measurement using read counts from *** that activity for an enzymatic reaction mainly depends upon the gene with the highest number of reads,and weighting the reads on gene length and ratio,a new expression value is calculated for the node as a *** expression at node level is then applied to the *** prostate cancer as model,we integrate RNA-seq data from two patient cohorts with metabolism data from *** we show that FunHoP gives more consistent pathways that are easier to interpret *** and documentation for running FunHoP can be found at https://***/kjerstirise/FunHoP.

关键词： Homologous proteins Metabolic network Pathway visualization and analysis RNA-seq KEGG Cytoscape

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Identification and substrate prediction of new Fragaria x ananassa aquaporins and expression in different tissues and during strawberry fruit development

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Horticulture Research 2018年第1期5卷 737-750页

作者： britt Merlaen Ellen De Keyser Marie-Christine Van Labeke Plant Production Faculty of Bioscience EngineeringGhent UniversityCoupure Links 6539000GentBelgium Flanders Research Institute for Agriculture Fisheries and Food(ILVO) Plant Sciences UnitCaritasstraat 399090MelleBelgium

The newly identified aquaporin coding sequences presented here pave the way for further insights into the plant–water relations in the commercial strawberry(Fragaria x ananassa).Aquaporins are water channel proteins that allow water to cross(intra)cellular *** Fragaria x ananassa,few of them have been identified hitherto,hampering the exploration of the water transport regulation at cellular ***,we present new aquaporin coding sequences belonging to different subclasses:plasma membrane intrinsic proteins subtype 1 and subtype 2(PIP1 and PIP2)and tonoplast intrinsic proteins(TIP).The classification is based on phylogenetic analysis and is confirmed by the presence of conserved ***-specific signature sequences(SSSSs)and specificity-determining positions(SDPs)predict the substrate specificity of each new *** profiling in leaves,petioles and developing fruits reveals distinct patterns,even within the same(sub)*** profiles range from leaf-specific expression over constitutive expression to fruit-specific *** upregulation and downregulation during fruit ripening *** specificity and expression profiles suggest that functional specialization exists among aquaporins belonging to a different but also to the same(sub)class.

关键词： Fragaria straw fir

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miRNA expression profiles in cerebrospinal fluid and blood of patients with Alzheimer’s disease and other types of dementia-an exploratory study

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Translational Neurodegeneration 2016年第1期5卷 40-51页

作者： Sofie Sølvsten Sørensen Ann-britt Nygaard Thomas Christensen Department of Neurology Copenhagen University HospitalNordsjællands HospitalDyrehavevej 293400 HillerødDenmark Department of Clinical Biochemistry Copenhagen University HospitalNordsjællands HospitalDyrehavevej 293400 HillerødDenmark

Background:MicroRNAs(miRNAs)are small non-coding RNA molecules that function as posttranscriptional regulators of gene *** of miRNAs in cerebrospinal fluid(CSF)and blood have just started gaining attention as a novel diagnostic tool for various neurological *** purpose of this exploratory investigation was to analyze the expression of miRNAs in CSF and blood of patients with Alzheimer’s disease(AD)and other neurodegenerative disorders in order to identify potential miRNA biomarker candidates able to separate AD from other types of ***:CSF was collected by lumbar puncture performed on 10 patients diagnosed with AD and 10 patients diagnosed with either vascular dementia,frontotemporal dementia or dementia with Lewy *** samples were taken immediately *** RNA was extracted from cell free fractions of CSF and plasma,and a screening for 372 known miRNA sequences was carried out by real time quantitative polymerase chain reactions(miRCURY LNA™Universal RT miRNA PCR,Polyadenylation and cDNA synthesis kit,Exiqon).Results:Fifty-two miRNAs were detected in CSF in at least nine out of ten patients in both *** these,two miRNAs(let-7i-5p and miR-15a-5p)were found significantly up-regulated and one miRNA(miR-29c-3p)was found significantly down-regulated in patients with AD compared to *** hundred and sixty-eight miRNAs were frequently detected in the blood,among which miR-590-5p and miR-142-5p were significantly up-regulated and miR-194-5p was significantly down-regulated in AD patients compared to ***:Detection of miRNA expression profiles in blood and in particular CSF of patients diagnosed with different types of dementia is feasible and it seems that several expressional differences between AD and other dementia types do exist when measured in a clinically relevant *** this explorative pilot study,the deregulated miRNAs in CSF of AD patients may be associated with relevant targ

关键词： Alzheimer’s disease Dementia Neurodegenerative disease miRNA expression Diagnostic biomarker Cerebrospinal fluid Blood

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Follicular dendritic cell sarcoma of the liver:unusual presentation of a rare tumor and literature review

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Hepatobiliary & Pancreatic Diseases International 2011年第4期10卷 443-445页

作者： Paulo N Martins Sanjay Reddy Ann-britt Martins Marcelo Facciuto Department of Surgery Division of Hepatobiliary Surgery and TransplantationNew York Medical CollegeNew YorkUSA Department of Surgery New York Medical CollegeSt.Vincent's Catholic Medical CenterNew YorkUSA

BACKGROUND:Hepatic follicular dendritic cell (FDC) sarcoma is an extremely rare *** commonly,FDC sarcoma presents as a solitary mass in lymph nodes,however,several extra-nodal locations have been ***:We report a case of a 53-year-old female who presented with symptoms of abdominal pain,fever,anemia,and *** an extensive review of the literature,we have found only 12 cases of hepatic FDC ***:The tumor was 11.5 cm in diameter and composed of spindle and epithelioid cells with ovoid nuclei and associated with mixed inflammatory *** stains were positive for CD35 and *** patient underwent a left hepatic ***:Liver follicular dendritic cell sarcoma is a very rare *** cases present with abdominal pain and weight loss,and most of them can be managed by hepatic resection with excellent short-term outcomes.

关键词： follicular dendritic cell tumor liver neoplasms sarcoma jaundice

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Antibiotic-loaded amphora-shaped pores on a titanium implant surface enhance osteointegration and prevent infections

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Bioactive Materials 2021年第8期6卷 2331-2345页

作者： Viviane Ständert Kai Borcherding Nicole Bormann Gerhard Schmidmaier Ingo Grunwald britt Wildemann Julius Wolff Institute BIH Center for Regenerative TherapiesCharitéUniversitätsmedizin BerlinCorporate Member of Freie Universität BerlinHumboldt-Universität zu BerlinBerlin Institute of Health13353BerlinGermany Department of Adhesive Bonding Technology and Surfaces Fraunhofer Institute for Manufacturing Technology and Advanced Materials(IFAM)28359BremenGermany Center for Orthopedics Trauma Surgery and Spinal Cord InjuryHTRG-Heidelberg Trauma Research GroupHeidelberg University Hospital69118HeidelbergGermany Industrial and Environmental Biology Hochschule Bremen-City University of Applied Sciences28199BremenGermany Experimental Trauma Surgery Department of TraumaHand and Reconstructive SurgeryJena University HospitalFriedrich Schiller University Jena07747JenaGermany

Artificial prostheses for joint replacement are indispensable in orthopedic ***,the implanted surface is attractive to not only host cells but also *** enable better osteointegration,a mechanically stable porous structure was created on a titanium surface using laser treatment and metallic silver particles were embedded in a hydrophilic titanium oxide layer on *** laser structuring resulted in unique amphora-shaped *** to their hydrophilic surface conditions and capillary forces,the pores can be loaded preoperative with the antibiotic of choice/need,such as *** and differentiation assays with primary human osteoblast-like cells revealed no negative effect of the surface modification with or without gentamicin *** in vivo biocompatibility study showed significantly enhanced osteointegration as measured by push-out testing and histomorphometry 56 days after the implantation of the K-wires into rat *** a *** infection model,the porous,silver-coated K-wires slightly reduced the signs of bone destruction,while the wires were still colonized after 28 *** the amphora-shaped pores with gentamicin significantly reduced the histopathological signs of bone destruction and no bacteria were detected on the *** together,this novel surface modification can be applied to new or established orthopedic *** enables preoperative loading with the antibiotic of choice/need without further equipment or post-coating,and supports osteointegration without a negative effect of the released dug,such as gentamicin.

关键词： Titanium implants Laser structuring Amphora-shaped pores Gentamicin loading Antimicrobial silver particles

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