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PCR-HRM for Genomic Surveillance of SARS-CoV-2: A Variant Detection Tool in Côte d’Ivoire, West Africa

American Journal of Molecular Biology 2024年第3期14卷 166-185页

作者： Aboubacar Sylla Solange Kakou-Ngazoa Tata Gniré Safiatou Coulibaly Yakoura Karidja Ouattara Mireille Sylvie Kouamé-Sina Zeinab Ouattara David Ngolo Coulibaly brice kouakou bla Mireille Dosso Molecular Biology Platform Pasteur Institute Cte dIvoire Abidjan Cte dIvoire Laboratory of Biology and Health UFR Biosciences University Flix Houphout Boigny Abidjan Cte dIvoire Genetic and Cancer Laboratory Molecular Genetics Platform Pasteur Institute Cte dIvoire Abidjan Cte dIvoire Epidemic Virus Department Pasteur Institute Cte dIvoire Abidjan Cte dIvoire

The rise of new viruses, like SARS-CoV-2 causing the COVID-19 outbreak, along with the return of antibiotic resistance in harmful bacteria, demands a swift and efficient reaction to safeguard the health and welfare of the global population. It is crucial to have effective measures for prevention, intervention, and monitoring in place to address these evolving and recurring risks, ensuring public health and international security. In countries with limited resources, utilizing recombinant mutation plasmid technology in conjunction with PCR-HRM could help differentiate the existence of novel variants. cDNA synthesis was carried out on 8 nasopharyngeal samples following viral RNA extraction. The P1 segment of the SARS-CoV-2 Spike S protein was amplified via conventional PCR. Subsequently, PCR products were ligated with the pGEM-T Easy vector to generate eight recombinant SARS-CoV-2 plasmids. Clones containing mutations were sequenced using Sanger sequencing and analyzed through PCR-HRM. The P1 segment of the S gene from SARS-CoV-2 was successfully amplified, resulting in 8 recombinant plasmids generated from the 231 bp fragment. PCR-HRM analysis of these recombinant plasmids differentiated three variations within the SARS-CoV-2 plasmid population, each displaying distinct melting temperatures. Sanger sequencing identified mutations A112C, G113T, A114G, G214T, and G216C on the P1 segment, validating the PCR-HRM findings of the variations. These mutations led to the detection of L452R or L452M and F486V protein mutations within the protein sequence of the Omicron variant of SARS-CoV-2. In summary, PCR-HRM is a vital and affordable tool for distinguishing SARS-CoV-2 variants utilizing recombinant plasmids as controls.

关键词： Genomic Surveillance SARS-CoV-2 PCR-HRM Variants Côte d’Ivoire

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Post-exercise heart rate variability recovery after 800-m endurance run load among Cameroonian adolescent's males

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Sports Medicine and Health Science 2023年第4期5卷 283-289页

作者： Jerson Mekoulou Ndongo Elysee Claude Bika Lele Wiliam Richard Guessogo Wiliam Mbang Mbian Clarisse Noel Ayina Ayina Jessica Guyot Christian Ngongang Ouankou Abdou Temfemo Loick Pradel Kojom Foko Ernest Tchoudjin Louis-Georges Gassina Bienvenu Bongue Frederic Roche Samuel Honore Mandengue Peguy brice Assomo-Ndemba Physical Activities and Sport Physiology&Medicine Unit Faculty of ScienceUniversity of DoualaCameroon Faculty of Sciences University of DoualaCameroon National Institute of Youth and Sports Yaounde University of Yaounde ICameroon Mines Saint-Etienne INSERMU1059 Sainbiose42023Universit Jean Monnet Saint-EtienneFrancee Faculty of Medicine and Pharmaceutical SciencesUniversity of DschangCameroon Faculty of Medicine and Pharmaceutical Sciences The University of DoualaCameroon Faculty of Medicine and Biomedical Science University of Yaounde ICameroon

The 800-m(m)run is part of Physical Education classes in Cameroon,after which arrhythmias may occur during ***,this study aimed at determining relationship between 800-m run loads on cardiac autonomic recovery among school ***-two male adolescents(aged[17-1]years)performed ***-exercise heart rate variability(HRV)was recorded during 5-min(min)(HRV5-min)and 15-min(HRV15-min)in time:Standard deviation of normal to normal(SDNN);Root mean square of successive differences(RMSSD)and frequency domain(LH:Low frequency,HF:High frequency,TP:Total power).Rating of Perceived Exertion(RPE)and blood lactate concentration(bla)were measured after *** HRV5-min,RPE was associated with SDNN(r=-0.44,pbla was correlated with SDNN(r=-0.38,pbla was correlated with RMSSD(r=-0.53,pbla was associated with HF(r=-0.55,p<0.001),TP(r=-0.50,p<0.01)and RPE with LF(r=-0.51,p<0.01),HF(r=-0.50,p<0.01),TP(r=-0.49,p<0.01).In addition,exercise duration was negatively linked to HF(r=-0.36,p<0.05).This study outlined that in untrained adolescents an increase of 800-m loads is associated with a slow vagal indexes of HRV during the recovery.

关键词： Heart rate variability Recovery 800-m load Adolescents Physical education classes

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